标签归档：xp

Whatman 沃特曼 GD/XP针头式滤器6970-2504、6994-2504

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Whatman 沃特曼 GD/XP针头式滤器

简要描述：

Whatman 沃特曼 GD/XP针头式滤器适合于过滤要求无机离子分析的样品，因为离子提取水平减到非常低的程度；对于要求蛋白吸附力低的使用者，它是一个很好的选择。GD/XP针头式滤器有2层由20 μm和5 μm聚丙烯组成的预滤层。Z后一层膜可供选择，位于预滤层下面。

GD/XP针头式滤器

Whatman GD/XP一次性针头式滤器适合于过滤要求无机离子分析的样品，因为离子提取水平减到非常低的程度；对于要求蛋白吸附力低的使用者，它是一个很好的选择。
GD/XP针头式滤器有2层由20 μm和5 μm聚丙烯组成的预滤层。zui后一层膜可供选择，位于预滤层下面。

应用

  • HPLC样品制备
  • 食品和饮料痕量金属元素样品制备
  • 环境污染物重金属样品制备

 

CST Anti-GFP （D5.1） XP抗体2956

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CST Anti-GFP （D5.1） XP抗体

  • 产品型号：  2956
  • 简单描述
  • CST Anti-GFP （D5.1） XP抗体Isotype：Rabbit IgGApplications：W IHC-P IF-IC FReactivity：AllSensitivity：Transfected Only
详细介绍

CST Anti-GFP （D5.1） XP抗体

Specificity / Sensitivity

GFP （D5.1） XP® Rabbit mAb detects GFP-tagged proteins exogenously expressed in cells.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of GFP.

Western Blotting

CST Anti-GFP （D5.1） XP抗体2956

Western blot analysis of extracts from HCC827 cells, untransfected or GFP-transfected, using GFP （D5.1） XP® Rabbit mAb.

IHC-P （paraffin）

CST Anti-GFP （D5.1） XP抗体2956

Immunohistochemical analysis of paraffin-embedded HCC827 cells, untransfected （left） or GFP-transfected （right）, using GFP （D5.1） XP® Rabbit mAb.

Flow Cytometry

CST Anti-GFP （D5.1） XP抗体2956

Flow cytometric analysis of HCC827 cells, untransfected （blue） or GFP-transfected （green）, using GFP （D5.1） XP® Rabbit mAb.

 

IF-IC

CST Anti-GFP （D5.1） XP抗体2956

Confocal immunofluorescent analysis of COS-7 cells expressing a GFP-tagged fusion protein （green） using GFP （D5.1） XP® Rabbit mAb （red）. Blue pseudocolor = DRAQ5® #4084 （fluorescent DNA dye）.

CST Anti-GFP （D5.1） XP抗体

Background

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties.

The green-fluorescent protein （GFP） functions as a bioluminescence energy transfer acceptor in the jellyfish Aequorea that maximally absorbs light at 395 nm and has an emission spectrum that peaks at 509 nm. GFP has become a very useful tool as a fusion protein that reports gene expression, traces cell lineage and defines subcellular protein localization （1）.

  1. Miyawaki, A. et al. （2003） Curr Opin Chem Biol 7, 557-62.

Application References

  • Matheny, R.W. and Nindl, B.C. （2011） Endocrinology 152, 1923-34. Applications: Western Blotting

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

  • 2955 GFP （4B10） Mouse mAb
  • 2372 β-Gal （14B7） Mouse mAb
  • 2367 HA-Tag （6E2） Mouse mAb
  • 2365 His-Tag Antibody
  • 2276 Myc-Tag （9B11） Mouse mAb
  • 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
  • 7074 Anti-rabbit IgG, HRP-linked Antibody
  • 7720 Prestained Protein Marker, Broad Range （Premixed Format）
  • 7727 Biotinylated Protein Ladder Detection Pack
  • 7003 20X LumiGLO® Reagent and 20X Peroxide

For Research Use Only. Not For Use In Diagnostic Procedures.