invitrogen MPK1096 Neon Transfection System 内容及储存• 3 × 1 ml Resuspension Buffer R• 3 × 1 ml Resuspension Buffer T• 2 × 150 ml Electrolytic Buffer E• 96 × 10 µl Neon® tips• 20 Neon® ele
详细介绍
invitrogen MPK1096 Neon Transfection System 10 µL Kit
描述
The Neon® Transfection System 10 µL Kit is designed specifically for use with the Neon® Transfection System. Use this kit for transfection volumes of 10 µL, containing 5 × 104–2 × 105adherent cells or 1 × 105–5 × 105 suspension cells. Cells that have been transfected using the included 10 µL Neon® Tips are ready to be washed and plated into a multi-well culture dish
invitrogen MPK1096 Neon Transfection System 10 µL Kit
规格
Cell Type:
Established Cell Lines, Hard-to-Transfect Cells, Primary Cells, Stem Cells
Effectene Transfection Reagent effectene转染试剂对各种类型的真核细胞DNA转染，尤其适用于原代细胞可在血清存在的情况下进行转染适合用于原代细胞或敏感的细胞系高转染效率而且较低的DNA使用量Low endotoxin level with <10 eu>
详细介绍
Effectene Transfection Reagent effectene转染试剂
Principle
Effectene Transfection Reagent is an innovative non-liposomal lipid formulation that is used in conjunction with a special DNA-condensing enhancer and optimized buffer to achieve high transfection efficiencies. The enhancer first condenses the DNA molecules and Effectene Reagent subsequently coats them with cationic lipids （see figure “Model of Effectene Principle”）, providing a particularly efficient way of transferring DNA into eukaryotic cells. Effectene Reagent offers significant advantages over many liposome reagents and other transfection methods （1, 2）.
Procedure
The Effectene procedure has two steps. DNA is first mixed with Enhancer and a buffer that provides optimal salt conditions for efficient DNA condensation. This step requires just 2–5 minutes. Effectene Reagent is then added and the mixture is incubated for 5–10 minutes to allow Effectene–DNA complexes to form. The complexes are mixed with growth medium （which can contain serum and antibiotics）, and added directly to the cells. The cells are then incubated until harvested and analyzed for gene expression （see flowchart “Effectene Transfection Reagent”）.
Applications
Effectene Reagent is suitable for transient and stable transfection of a broad range of cell types （see figures “High Transfection Efficiencies Using Effectene Reagent” and “Transfection of Oligonucleotides Using Effectene Reagent”）. Because transfection can be performed in the presence of serum and requires low amounts of DNA （see figure “Serum and DNA Quantity vs. Transfection Efficiency”）, cytotoxicity is minimal. This makes Effectene Reagent particularly effective for primary cells （see figure “Effectene Reagent with Primary Cells”）. A searchable list of cell lines and primary cells successfully transfected using Effectene Reagent, as well as customer-developed transfection protocols, is available at the Transfection Tools web site — www.qiagen.com/TransfectionTools. View cell lines successfully transfected with this product.
Effectene Transfection Reagent effectene转染试剂
High-throughput transfection
The application of recombinant DNA technology to fields such as drug discovery and development has led to an increased need for high-throughput transfection. Transfection using Effectene Reagent requires low amounts of DNA and minimal handling, and in addition, removal of transfection complexes is not required, making this reagent highly suitable for high-throughput screening. Effectene Reagent provides outstanding transfection efficiencies, excellent reproducibility, and minimal cytotoxicity in high-throughput transfection, and is available in bulk quantities.
Cited References
1. Ausubel, F.M., et al. eds. （1991） Current Protocols in Molecular Biology, Wiley Interscience, New York. 2. Sambrook, J., Fritsch, E.F., and Maniatis, T., eds. （1989） Molecular cloning — a laboratory manual, 2nd Ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor.
Images
Model of Effectene Principle High Transfection Efficiencies Using Effectene Reagent Transfection of Oligonucleotides Using Effectene Reagent Effectene Transfection Procedure Serum and DNA Quantity vs. Transfection Efficiency Effectene Reagent with Primary Cells
提高转染效率的一个简单方法就是提高质粒DNA和转染试剂混合物的用量。然而，由于大多数的转染试剂在转染期间会破坏细胞，因此仅仅提高用量并不是一个明智的选择。而Xfect Transfection Reagent是理想的选择。Xfect是可生物降解的转染聚合物试剂，以毒性低和转染效率高的特性从2,300种侯选物中脱颖而出。Xfect可以提高您的转染效率，同时不会杀死细胞。点击链接观看操作视频Xfect Single Shots Video
Model of Effectene Principle 
High Transfection Efficiencies Using Effectene Reagent 
Transfection of Oligonucleotides Using Effectene Reagent 
Effectene Transfection Procedure 
Serum and DNA Quantity vs. Transfection Efficiency 
Effectene Reagent with Primary Cells
