qiagen QIAamp DNA FFPE Tissue Kit(石蜡组织DNA提取试剂盒)货号56404从石蜡包埋组织中纯化基因组DNA快速纯化高品质、即用型DNA重复性好,产量高*去除污染物和抑制剂
详细介绍
qiagen QIAamp DNA FFPE Tissue Kit(石蜡组织DNA提取试剂盒)货号56404
QIAamp DNA FFPE Tissue Kit专为从福尔马林固定、石蜡包埋组织中纯化DNA而设计。该试剂盒应用特殊的裂解方式脱离组织切片中的DNA,克服了福尔马林交联造成的抑制效应。试剂盒通过QIAamp MinElute离心柱纯化高品质DNA,洗脱体积小。QIAamp DNA FFPE Tissue Kit可在QIAcube全自动核酸纯化仪上自动纯化DNA。
qiagen QIAamp DNA FFPE Tissue Kit(石蜡组织DNA提取试剂盒)货号56404
对从FFPE组织样本中纯化的DNA进行PCR分析。
使用QIAamp DNA FFPE Tissue Kit,从不同的FFPE组织样本中纯化基因组DNA。采用real-time定量PCR扩增prnp基因(78 bp、464 bp和727 bp)产物。
Efficient: no background effects like cell activation or apoptosis
Flexible: tested for variety of tissue types
The MACS® Tissue Storage Solution has been developed to allow an optimized storage of fresh organ and tissue samples without showing background effects like cell activation or apoptosis induction. It has been tested and validated on a variety of human and murine tissues including tumors, skin, heart, spleen, brain, and skeletal muscle.
可靠的硅胶膜技术,利用便捷的离心柱或96孔板形式,确保了快速可重复的DNA纯化,不再需要有机抽提和乙醇沉淀(参见”DNeasy Mini and 96 procedures”)。样本首先用蛋白酶K裂解。调整缓冲条件,以提供的DNA结合条件并将裂解物上样到DNeasy Mini离心柱或DNeasy 96孔板上。在离心过程中,DNA选择性地结合到DNeasy膜上,污染物通过。余下的污染物和酶抑制剂将在两个高效的洗涤步骤中被去除。然后,用水或缓冲液将DNA洗脱,待用。
Tissue DNA Kit(50)组织DNA提取试剂盒规格:50次现货提供一个简单、快速、有效的从多种动物组织样品提取基因组DNA的方法。 在组织样品经消化裂解后可在30分钟内完成提取工作。 每次可处理小于30mg的样品,提取过程无需用到有毒的酚氯仿等有机溶剂抽提,也不需用到耗时的醇类(异丙醇/乙醇)沉淀。纯化的DNA可直接用于PCR、酶切、杂交等下游实验
he E.Z.N.A.® Tissue DNA Kit offers a simple, rapid, and cost-effective method for the isolation of DNA from a wide variety of sample sources include fresh or frozen animal cells and tissues. After cell lysis, the DNA purification process can be completed in fewer than 30 minutes. Up to 30 mg of tissue at a time can be easily processed using the simple E.Z.N.A.® Tissue DNA protocol. With the spin-column based kit, multiple samples can be processed in parallel. There is no need for phenol/chloroform extractions or time-consuming steps such as precipitation with isopropanol or ethanol. DNA purified using the E.Z.N.A.® Tissue DNA Kit is ready for most downstream applications such as PCR, Southern blots, and restriction enzyme digestion.
Rapid – DNA isolation in under 20 minutes (after lysis)
Reliable – Optimized buffers guarantee pure DNA every time
Safe – No organic extractions
High-quality – Purified DNA suitable for most applications