Streptavidin, Horseradish Peroxidase辣根过氧化物酶标记的链亲和素VECTOR SA-5704

Streptavidin, Horseradish Peroxidase辣根过氧化物酶标记的链亲和素

  • 产品型号:  VECTOR SA-5704
  • 简单描述
  • Using biotinylated primary or secondary intermediates and peroxidase-labeled streptavidin, antigens can be localized in histological sections, cytospin preparations, or smears
详细介绍

Avidin and streptavidin reagents are powerful tools to detect or purify biotinylated proteins, nucleic acids, and other macromolecules.

Vector Laboratories’ enzyme-conjugated avidin and streptavidin are produced with the highest specific activity enzymes in optimal ratios. Specific covalent linkages are chosen to provide stable, highly active conjugates.

These enzyme conjugates are suitable for use in solid-phase assays, tissue/cell staining systems, and blotting applications.

Horseradish Peroxidase Streptavidin is produced by our own coupling procedure which preserves the high specific activity of the peroxidase. Using biotinylated primary or secondary intermediates and peroxidase-labeled streptavidin, antigens can be localized in histological sections, cytospin preparations, or smears. In addition, sensitive detection systems for a variety of solid-phase assays utilizing microtitration plates or nitrocellulose have been developed using this conjugate. Horseradish Peroxidase Streptavidin is supplied as a concentrate (1mg/ml) or as a ready-to-use (R.T.U.) stabilized solution (100 ml, 1µg/ml) in a bottle fitted with a drop dispenser top.

 

CERTIFICATE OF ANALYSIS
Product R.T.U HORSERADISH PEROXIDASE STREPTAVIDIN
Catalog No. SA-5704
Amount 100 ml
Lot No. X1103
Concentration 1.0 μg/ml active conjugate*
1.5
Molar ratio of active conjugate
Storage Conditions Refrigerate; DO NOT FREEZE
* This product is provided at a ready-to-use concentration in a solution which
preserves the activity on the conjugate when stored as recommended.
For most applications the recommended incubation time is 30 minutes.
Do not use sodium azide with this product since it is an inhibitor of horseradish
peroxidase.
FOR LABORATORY USE.

 

 

Streptavidin, Horseradish Peroxidase, R.T.U.VECTOR试剂代理SA-5704

Streptavidin, Horseradish Peroxidase, R.T.U.

  • 产品型号:  VECTOR试剂代理SA-5704
  • 简单描述
  • Streptavidin, Horseradish Peroxidase, R.T.U. (Ready-to-Use) VECTOR试剂供应,*,咨询
详细介绍

Avidin and streptavidin reagents are powerful tools to detect or purify biotinylated proteins, nucleic acids, and other macromolecules.

Vector Laboratories’ enzyme-conjugated avidin and streptavidin are produced with the highest specific activity enzymes in optimal ratios. Specific covalent linkages are chosen to provide stable, highly active conjugates.

These enzyme conjugates are suitable for use in solid-phase assays, tissue/cell staining systems, and blotting applications.

Horseradish Peroxidase Streptavidin is produced by our own coupling procedure which preserves the high specific activity of the peroxidase. Using biotinylated primary or secondary intermediates and peroxidase-labeled streptavidin, antigens can be localized in histological sections, cytospin preparations, or smears. In addition, sensitive detection systems for a variety of solid-phase assays utilizing microtitration plates or nitrocellulose have been developed using this conjugate. Horseradish Peroxidase Streptavidin is supplied as a concentrate (1mg/ml) or as a ready-to-use (R.T.U.) stabilized solution (100 ml, 1µg/ml) in a bottle fitted with a drop dispenser top.

 

Streptavidin标记物快速纯化柱—Capturem™ 膜技术酶试剂盒Takara


Streptavidin标记物快速纯化柱—Capturem 膜技术
品牌 Code No. 产品名称 包装量 价格(元) 说明书 数量
Clontech 635733 Capturem Streptavidin Miniprep Columns 20 Columns ¥4,638 Streptavidin标记物快速纯化柱—Capturem™ 膜技术 Streptavidin标记物快速纯化柱—Capturem™ 膜技术 Streptavidin标记物快速纯化柱—Capturem™ 膜技术
Clontech 635734 Capturem Streptavidin 96-Well Plate 1 × 96 well plate ¥8,935 Streptavidin标记物快速纯化柱—Capturem™ 膜技术 Streptavidin标记物快速纯化柱—Capturem™ 膜技术 Streptavidin标记物快速纯化柱—Capturem™ 膜技术
收藏产品 加入购物车
 
■ 产品简介
Capturem Streptavidin——利用链霉亲和素快速富集和纯化生物素化物质。
链霉亲和素是一种来源于链霉菌Streptomyces avidinii的细胞壁蛋白质,与生物素有非常强的亲和力,这一互作特性在生命科学领域被广泛应用于富集等相关研究。Capturem Streptavidin mini 纯化柱和96-well 纯化板是将链霉亲和素固定在高容量的Capturem膜上,从而在室温条件下就可以对生物素化的靶标实现快速、高质量的纯化和富集,整个操作从上样到洗脱仅需5~15分钟。此外,还可以利用Capturem Streptavidin来捕获或pull down靶标分子或复合物。
 
■ 产品特点
· 简单、快速的操作流程:室温下操作即可,小量纯化仅需5 min,96-well板仅需15 min
· 产物浓度高:少量的洗脱液即可实现高浓度洗脱
· 高质量:样品停留时间短,从而有效地防止了样品聚集、失活,或者样品中复合物解离
· 高重复性:每个纯化柱的纯化结果有高度的一致性
· 一次性使用:新型膜技术搭配一次性离心柱有效降低了污染和残留的风险
 
■ 应用
· 生物素化蛋白质的纯化或富集
· Pull down与生物素化的配体相结合的蛋白质或oligo
 
Capturem Streptavidin对不同样品的载量和操作时间
  生物素标记的抗体 生物素标记的BSA 生物素标记的ssDNA 游离的生物素 纯化时间
Capturem Streptavidin mini纯化柱和96-well 纯化板 20-40 μg >15 μg 1,000-1,500 ng >4,000 pmol 15 分钟
 
实验例
例1
Streptavidin标记物快速纯化柱—Capturem™ 膜技术
使用Capturem Streptavidin连续三次捕获抗体。图A–C.首先将含48 μg生物素化兔IgG的Binding Buffer上样至平衡好的Capturem Streptavidin spin column中,这时32.0 ± 1.4 μg (图B)的抗体被成功固定在膜上。然后洗涤一次,再将使用Binding Buffer稀释的含20%小鼠血清且同时加入spiked-in靶抗体(约100 μg山羊抗兔抗体)的杂交瘤培养基加入到Capturem Streptavidin spin column中,分别使用Binding Buffer和PBS先后洗涤两次,最后使用1.0 M甘氨酸洗脱3次,从生物素化的捕捉抗体上洗脱获得高纯度的靶抗体,收量约为42 ± 5 μg (图C)。
 
例2
Streptavidin标记物快速纯化柱—Capturem™ 膜技术
Capturem Streptavidin 96-well纯化板具有高度重复性。分别对生物素化oligo(图A)和生物素化BSA(图B)进行8次技术重复上样,并使用离心的方法进行靶标捕获。通过测定样品上样前后的OD值来对结合的分子进行定量。捕获操作按照标准的流程进行,15 min之内完成。
 
 
 
更多产品信息请点击:Streptavidin标记物快速纯化柱—Capturem™ 膜技术
 
 

页面更新:2020-04-02 14:10:48