上海金畔生物科技有限公司代理日本同仁化学 DOJINDO代理商全线产品，欢迎访问官网了解更多信息
特点：
·日本原装进口试剂盒
·固定和不固定都可使用
关联产品
BIOVISION Live-Dead Cell Staining Kit
BIOVISION Live-Dead Cell Staining Kit
BioVision的活-死细胞染色试剂盒提供了即用型试剂来方便地区分死细胞和活细胞。本染色试剂盒利用Live-Dye染料来对活细胞染色，这是一种能穿透细胞的绿色荧光染料（Ex/Em = 488/518 nm）。死细胞可以简单的用碘化吡啶（PI）染色，一种不能透过细胞膜的红色荧光染料（Ex/Em = 488/615）。染上色的活细胞和死细胞通过使用了滤光片（检测FITC和罗丹明）的荧光显微镜可以直接从视觉上区分开来。本试剂盒提供的试剂可以在24孔板上染色100次。
BIOVISION Live-Dead Cell Staining Kit
现货*！
Kit Summary:
? Detection method- Fluorescent microscopy (Ex/Em 488/518 nm) to detect staines live cells; (Ex/Em 488/615) to detect stained dead cells
? Sample type- Cell culture (adherent and suspension cells)
? Species reactivity- Mammalian
? Applications- Stained live and dead cells can easily be visualized by fluorescence microscopy.
Features & Benefits:
? Simple procedure; takes less than 20 minutes
? Fast and convenient
Kit components:
? Solution A (1 mM Live-Dye)
? Solution B (2.5 mg/ml PI)
? Staining Buffer
Description:
Distinguishing between live and dead cells is very important for investigation of growth control and cell death. The Live-Dead Cell Staining Kit provides the ready-to-use reagents for convenient discrimination between live and dead cells. The kit utilizes Live-Dye?, a cell-permeable green fluorescent dye (Ex/Em = 488/518 nm), to stain live cells. Dead cells can be easily stained by propidium iodide (PI), a cell non-permeable red fluorescent dye (Ex/Em = 488/615). Stained live and dead cells can be visualized by fluorescence microscopy using a band-pass filter (detects FITC and rhodamine). The kit provides sufficient reagents for 100 stainings using 24-well plate.
Storage Conditions:
-20°C
Shipping Conditions:
gel pack
USAGE: For Research Use Only! Not For Use in Humans.
Biovision活死细胞双染试剂盒Live-Dead Cell Staining Kit
上海金畔生物科技有限公司代理日本同仁化学试剂盒全线产品，欢迎访问日本同仁化学dojindo官网了解更多信息。
-Bacstain- CTC Rapid Staining Kit (for Flow cytometry)| 容 量 | メーカー希望 小売価格 |
富士フイルム 和光純薬 |
|---|---|---|
| 100 Assays | ￥25,700 | 348-91301 |
| 100 Assays | ･CTC ･Enhancing reagent A |
10 mg x3 100 μl x1 |
|---|
細菌を蛍光染色したい 1)従来のCTC染色に比べ高感度な検出が可能である。
2)少量小分け品であるため、試薬の調製が容易である。
※本品は、福岡県工業技術センター生物食品研究所との共同開発製品である。
※使用方法は、プロトコルをご覧ください。
1) A. W. Coleman, "Enhanced Detection of Bacteria in Natural Environments by Fluorochrome Staining of DNA", Limnol. Oceanogr., 1980, 25, 948.
2) E. Severin, J. Stellmach and H. -M. Nachtigal, "Fluorimetric Assay of Redox Activity in Cells", Anal. Chim. Acta, 1985, 170, 341.
3) G. G. Rodriguez, D. Phipps, K. Ishiguro and H. F. Ridgway, "Use of a Fluorescent Redox Probe for Direct Visualization of Actively Respiring Bacteria", Appl. Environ. Microbiol., 1992, 58(6), 1801.
4) G. Schaule, H. C. Flemming and H. F. Ridgway, "Use of 5-Cyano-2,3-ditolyl Tetrazolium Chloride for Quantifying Planktonic and Sessile Respiring Bacteria in Drinking Water", Appl. Environ. Microbiol., 1993, 59(11), 3850.
5) R. A. Bovill, J. A. Shalloross and B. M. Markey, "Comparison of the Fluorescent Redox Dye 5-Cyano-2,3-ditolyltetrazolium Chloride with p-Iodonitrotetrazolium Violet to Detect Metabolic Activity in Heat-stressed Listeria monocytogenes Cells", J. Appl. Bacteriol., 1994, 77(4), 353.
6) M. T. E. Suller and D. Lloyd, "Flow Cytometric Assesment of the Postantibiotic Effect of Methicillin on Staphylococcus aureus", Antimicrob. Agents Chemother., 1998, 42(5), 1195.
7) M. Kawai, N. Yamaguchi and M. Nasu "Rapid Enumeration of Physiologically Active Bacteria in Purified Water Used in the Pharmaceutical Manufacuturing Process", J. Appl. Microbiol., 1999, 86, 496.
8) N. Yamaguchi, M. Sasada, M. Yamanaka and M. Nasu, "Rapid Detection of Respiring Escherichia coli O157:H7 in Apple Juice, Milk and Ground Beef by Flow Cytometry", Cytometry , 2003, 54A, 27.
9) A. Kitaguchi, N. Yamagauchi and M. Nasu, "Enumeration of Respiring Pseudomonas spp. in Milk within 6 Hours by Fluorescence In Situ Hybridization Following Formazan Reduction", Appl. Environ. Microbiol., 2005, 71(5), 2748.
10)A. Hiraishi and N. Yoshida, "An Improved Redox Dye-Staining Method Using 5-Cyano-2,3-Ditoryl Tetrazolium Chloride for Detection of Metabolically Active Bacteria in Activated Sludge", Microbes Environ., 2004, 19(1), 61.
11)平石 明, 吉田 奈央子, “活性汚泥における培養不能な細菌の検出”, 月刊 海洋 培養不能細菌 -VNC研究の現状と課題–, 2003, 33, 48．
12)染谷 孝, “蛍光染色による土壌微生物の検出法”, 月刊 海洋 培養不能細菌 –VNC研究の現状と課題–, 2003, 33, 14．
| 保存条件: 冷蔵,遮光 | |
| 危険・有害 シンボルマーク |
 |
|---|---|
上海金畔生物科技有限公司代理日本同仁化学试剂盒全线产品，欢迎访问日本同仁化学dojindo官网了解更多信息。
–Bacstain– CTC Rapid Staining Kit (for Microscopy)| 容 量 | メーカー希望 小売価格 |
富士フイルム 和光純薬 |
|---|---|---|
| 100 Assays | ￥25,100 | 345-91311 |
| 100 Assays | ・CTC ・Enhancing Reagent B |
10 mg × 3 500 μl × 1 |
|---|
細菌を蛍光染色したい ・20 μL, 1000 μLマイクロピペット ・インキュベーター
・蛍光顕微鏡（blue excitation filter, red emission filter)
1) A. W. Coleman, "Enhanced Detection of Bacteria in Natural Environments by Fluorochrome Staining of DNA", Limnol. Oceanogr., 1980, 25, 948.
2) E. Severin, J. Stellmach and H. -M. Nachtigal, "Fluorimetric Assay of Redox Activity in Cells", Anal. Chim. Acta, 1985, 170, 341.
3) G. G. Rodriguez, D. Phipps, K. Ishiguro and H. F. Ridgway, "Use of a Fluorescent Redox Probe for Direct Visualization of Actively Respiring Bacteria", Appl. Environ. Microbiol., 1992, 58(6), 1801.
4) G. Schaule, H. C. Flemming and H. F. Ridgway, "Use of 5-Cyano-2,3-ditolyl Tetrazolium Chloride for Quantifying Planktonic and Sessile Respiring Bacteria in Drinking Water", Appl. Environ. Microbiol., 1993, 59(11), 3850.
5) R. A. Bovill, J. A. Shalloross and B. M. Markey, "Comparison of the Fluorescent Redox Dye 5-Cyano-2,3-ditolyltetrazolium Chloride with p-Iodonitrotetrazolium Violet to Detect Metabolic Activity in Heat-stressed Listeria monocytogenes Cells", J. Appl. Bacteriol., 1994, 77(4), 353.
6) M. T. E. Suller and D. Lloyd, "Flow Cytometric Assesment of the Postantibiotic Effect of Methicillin on Staphylococcus aureus", Antimicrob. Agents Chemother., 1998, 42(5), 1195.
7) M. Kawai, N. Yamaguchi and M. Nasu "Rapid Enumeration of Physiologically Active Bacteria in Purified Water Used in the Pharmaceutical Manufacuturing Process", J. Appl. Microbiol., 1999, 86, 496.
8) N. Yamaguchi, M. Sasada, M. Yamanaka and M. Nasu, "Rapid Detection of Respiring Escherichia coli O157:H7 in Apple Juice, Milk and Ground Beef by Flow Cytometry", Cytometry, 2003, 54A, 27.
9) A. Kitaguchi, N. Yamagauchi and M. Nasu, "Enumeration of Respiring Pseudomonas spp. in Milk within 6 Hours by Fluorescence In Situ Hybridization Following Formazan Reduction", Appl. Environ. Microbiol., 2005, 71(5), 2748.
10)A. Hiraishi and N. Yoshida, "An Improved Redox Dye-Staining Method Using 5-Cyano-2,3-Ditoryl Tetrazolium Chloride for Detection of Metabolically Active Bacteria in Activated Sludge", Microbes Environ., 2004, 19(1), 61.
11)平石 明，吉田 奈央子，“活性汚泥における培養不能な細菌の検出”，月刊 海洋 培養不能細菌 -VNC研究の現状と課題-，2003, 33, 48．
12)染谷 孝， “蛍光染色による土壌微生物の検出法”， 月刊 海洋 培養不能細菌 -VNC研究の現状と課題-， 2003, 33, 14．
| 保存条件: 冷蔵,遮光 |