标签归档：recombinant

人肝细胞生长因子 Recombinant Human HGF100-39

发表于由

人肝细胞生长因子 Recombinant Human HGF

  • 产品型号：  100-39
  • 简单描述
  • Peprotech Recombinant Human HGF Synonyms:Scatter Factor (SF), Hepatopoietin (HPTA)
详细介绍

 

Recombinant Human HGF

Catalog Number:
100-39
Synonyms:
Scatter Factor （SF）, Hepatopoietin （HPTA）
Description:
HGF is a mesenchymally derived potent mitogen for mature parenchymal hepatocyte cells and acts as a growth factor for a broad spectrum of tissues and cell types. HGF signals through a transmembrane tyrosine kinase receptor known as MET. Activities of HGF include induction of cell proliferation, motility, morphogenesis, inhibition of cell growth, and enhancement of neuron survival. HGF is a crucial mitogen for liver regeneration processes, especially after partial hepatectomy and other liver injuries. Human and murine HGF are cross-reactive. Human HGF is expressed as a linear 697 amino acid polypeptide precursor glycoprotein. Proteolytic processing of this precursor generates the biologically active form of HGF, which consists of two polypeptide chains （α-chain and β-chain） held by a single disulfide bond resulting in formation of a biologically active heterodimer. The α-chain consists of 463 amino acid residues and four kringle domains. The β-chain consists of 234 amino acid residues.*Manufactured using （BTI-Tn-5B1-4） cells under license from Boyce Thompson Institute for Plant Research, Inc.
AA Sequence:

alpha chain: QRKRRNTIHE FKKSAKTTLI KIDPALKIKT KKVNTADQCA NRCTRNKGLP FTCKAFVFDK ARKQCLWFPF NSMSSGVKKE FGHEFDLYEN KDYIRNCIIG KGRSYKGTVS ITKSGIKCQP WSSMIPHEHS FLPSSYRGKD LQENYCRNPR GEEGGPWCFT SNPEVRYEVC DIPQCSEVEC MTCNGESYRG LMDHTESGKI CQRWDHQTPH RHKFLPERYP DKGFDDNYCR NPDGQPRPWC YTLDPHTRWE YCAIKTCADN TMNDTDVPLE TTECIQGQGE GYRGTVNTIW NGIPCQRWDS QYPHEHDMTP ENFKCKDLRE NYCRNPDGSE SPWCFTTDPN IRVGYCSQIP NCDMSHGQDC YRGNGKNYMG NLSQTRSGLT CSMWDKNMED LHRHIFWEPD ASKLNENYCR NPDDDAHGPW CYTGNPLIPW DYCPISRCEG DTTPTIVNLD HPVISCAKTK QLR

beta chain: VVNGIP TRTNIGWMVS LRYRNKHICG GSLIKESWVL TARQCFPSRD LKDYEAWLGI HDVHGRGDEK CKQVLNVSQL VYGPEGSDLV LMKLARPAVL DDFVSTIDLP NYGCTIPEKT SCSVYGWGYT GLINYDGLLR VAHLYIMGNE KCSQHHRGKV TLNESEICAG AEKIGSGPCE GDYGGPLVCE QHKMRMVLGV IVPGRGCAIP NRPGIFVRVA YYAKWIHKII LTYKVPQS

Source:
（BTI‐Tn‐5B1‐4） Hi‐5 Insect cells*
Purity:
Greater than 98% by SDS-PAGE gel and HPLC analyses.
Biological Activity:
The ED50 was determined by the dose-dependent stimulation of the proliferation of monkey 4MBr-5 cells was found to be in the range of 20.0-40.0 ng/ml.
 
 
 
 
Cross Reactivity:
Dog, Monkey, Mouse, Rabbit, Rat, Human + Mouse, Human, Leech
Research Interests:
人肝细胞生长因子 Recombinant Human HGF100-39 Angiogenesis/ Cardiovascular 人肝细胞生长因子 Recombinant Human HGF100-39 Cancer
人肝细胞生长因子 Recombinant Human HGF100-39 Neurobiology 人肝细胞生长因子 Recombinant Human HGF100-39 Proliferation
人肝细胞生长因子 Recombinant Human HGF100-39 Stem Cells & Differentiation 人肝细胞生长因子 Recombinant Human HGF100-39 Wound Healing
 
 
Country of Origin:
USA
Research Use Only. Not for use in diagnostic or therapeutic procedures.
*Manufactured using （BTI-Tn-5B1-4） cells under license from the Boyce Thompson Institute for Plant Research, Inc.

FGF-4人细胞生长因子Recombinant Human FGF-4100-31

发表于由

FGF-4人细胞生长因子Recombinant Human FGF-4

  • 产品型号：  100-31
  • 简单描述
  • Recombinant Human FGF-4 Synonyms:Fibroblast Growth Factor-4, HST-1, transforming protein KS3, HBGF-4
详细介绍

 Description:

FGF-4 is a heparin binding growth factor that is a member of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-4 signals through the FGFR 1c, 2c, 3c, and 4. Recombinant human FGF-4 is a 19.7 kDa protein consisting of 182 amino acid residues.
AA Sequence:
GRGGAAAPTA PNGTLEAELE RRWESLVALS LARLPVAAQP KEAAVQSGAG DYLLGIKRLR RLYCNVGIGF HLQALPDGRI GGAHADTRDS LLELSPVERG VVSIFGVASR FFVAMSSKGK LYGSPFFTDE CTFKEILLPN NYNAYESYKY PGMFIALSKN GKTKKGNRVS PTMKVTHFLP RL
Source:
E.coli
Purity:
Greater than 95% by SDS-PAGE gel and HPLC analyses.
Biological Activity:
The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF-receptors is ≤ 0.5 ng/ml, corresponding to a specific activity of ≥ 2 x 106units/mg.
 
 
 
 
Cross Reactivity:
Mouse, Rat, Human, N/A, Pig
Research Interests:
FGF-4人细胞生长因子Recombinant Human FGF-4100-31 Angiogenesis/ Cardiovascular FGF-4人细胞生长因子Recombinant Human FGF-4100-31 FGF Superfamily
FGF-4人细胞生长因子Recombinant Human FGF-4100-31 Proliferation FGF-4人细胞生长因子Recombinant Human FGF-4100-31 Stem Cells & Differentiation
 
 
Country of Origin:
USA
Research Use Only. Not for use in diagnostic or therapeutic procedures.

FGF-4人细胞生长因子Recombinant Human FGF-4100-31

发表于由

FGF-4人细胞生长因子Recombinant Human FGF-4

  • 产品型号：  100-31
  • 简单描述
  • Recombinant Human FGF-4 Synonyms:Fibroblast Growth Factor-4, HST-1, transforming protein KS3, HBGF-4
详细介绍

 Description:

FGF-4 is a heparin binding growth factor that is a member of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-4 signals through the FGFR 1c, 2c, 3c, and 4. Recombinant human FGF-4 is a 19.7 kDa protein consisting of 182 amino acid residues.
AA Sequence:
GRGGAAAPTA PNGTLEAELE RRWESLVALS LARLPVAAQP KEAAVQSGAG DYLLGIKRLR RLYCNVGIGF HLQALPDGRI GGAHADTRDS LLELSPVERG VVSIFGVASR FFVAMSSKGK LYGSPFFTDE CTFKEILLPN NYNAYESYKY PGMFIALSKN GKTKKGNRVS PTMKVTHFLP RL
Source:
E.coli
Purity:
Greater than 95% by SDS-PAGE gel and HPLC analyses.
Biological Activity:
The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF-receptors is ≤ 0.5 ng/ml, corresponding to a specific activity of ≥ 2 x 106units/mg.
 
 
 
 
Cross Reactivity:
Mouse, Rat, Human, N/A, Pig
Research Interests:
FGF-4人细胞生长因子Recombinant Human FGF-4100-31 Angiogenesis/ Cardiovascular FGF-4人细胞生长因子Recombinant Human FGF-4100-31 FGF Superfamily
FGF-4人细胞生长因子Recombinant Human FGF-4100-31 Proliferation FGF-4人细胞生长因子Recombinant Human FGF-4100-31 Stem Cells & Differentiation
 
 
Country of Origin:
USA
Research Use Only. Not for use in diagnostic or therapeutic procedures.

Peprotech重组人TGF-β1细胞因子Recombinant Human TGF-β1 （mammalian derived）100-21

发表于由

Peprotech重组人TGF-β1细胞因子Recombinant Human TGF-β1 （mammalian derived）

  • 产品型号：  100-21
  • 简单描述
  • Recombinant Human TGF-β1 (mammalian derived)重组人TGF-β1细胞因子，详细产品信息请
详细介绍

 

Recombinant Human TGF-β1 （mammalian derived）

Catalog Number:
100-21
Synonyms:
Transforming Growth Factor-β1, Differentiation inhibiting factor, Cartilage-inducing factor
Description:
The three mammalian isoforms of TGF-β, TGF-β1, β2, β3, signal through the same receptor and elicit similar biological responses. They are multifunctional cytokines that regulate cell proliferation, growth, differentiation and motility as well as synthesis and deposition of the extracellular matrix. They are involved in various physiological processes including embryogenesis, tissue remodeling and wound healing. They are secreted predominantly as latent complexes which are stored at the cell surface and in the extracellular matrix. The release of biologically active TGF-β isoform from a latent complex involves proteolytic processing of the complex and /or induction of conformational changes by proteins such as thrombospondin-1. TGF-β1 is the most abundant isoform secreted by almost every cell type. It was originally identified for its ability to induce phenotypic transformation of fibroblasts and recently it has been implicated in the formation of skin tumors. Human TGF-β1 is a 25.0 kDa protein with each subunit containing 112 amino acid residues, linked by a single disulfide bond.
AA Sequence:
ALDTNYCFSS TEKNCCVRQL YIDFRKDLGW KWIHEPKGYH ANFCLGPCPY IWSLDTQYSK VLALYNQHNP GASAAPCCVP QALEPLPIVY YVGRKPKVEQ LSNMIVRSCK CS
Source:
HEK293 cells
Purity:
Greater than 98% by SDS-PAGE gel and HPLC analyses.
Biological Activity:
The ED50 was determined by TGF-beta1’s ability to inhibit the mouse IL-4-dependent proliferation of mouse HT-2 cells is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 107 units/mg.
 
 
 
 
Cross Reactivity:
Chicken, Cow, Dog, Frog, Monkey, Mouse, Pig, Rabbit, Rat, Human, Mink, Donkey, N/A
Research Interests:
Peprotech重组人TGF-β1细胞因子Recombinant Human TGF-β1 （mammalian derived）100-21 Angiogenesis/ Cardiovascular Peprotech重组人TGF-β1细胞因子Recombinant Human TGF-β1 （mammalian derived）100-21 Apoptosis
Peprotech重组人TGF-β1细胞因子Recombinant Human TGF-β1 （mammalian derived）100-21 Cancer Peprotech重组人TGF-β1细胞因子Recombinant Human TGF-β1 （mammalian derived）100-21 Immune System
Peprotech重组人TGF-β1细胞因子Recombinant Human TGF-β1 （mammalian derived）100-21 Inflammation Peprotech重组人TGF-β1细胞因子Recombinant Human TGF-β1 （mammalian derived）100-21 Proliferation
Peprotech重组人TGF-β1细胞因子Recombinant Human TGF-β1 （mammalian derived）100-21 Stem Cells & Differentiation Peprotech重组人TGF-β1细胞因子Recombinant Human TGF-β1 （mammalian derived）100-21 TGF-β Superfamily
Peprotech重组人TGF-β1细胞因子Recombinant Human TGF-β1 （mammalian derived）100-21 Wound Healing Peprotech重组人TGF-β1细胞因子Recombinant Human TGF-β1 （mammalian derived）100-21 Allergy
 
 
Country of Origin:
USA

Peprotech重组人TGF-β1细胞因子Recombinant Human TGF-β1 （mammalian derived）100-21

发表于由

Peprotech重组人TGF-β1细胞因子Recombinant Human TGF-β1 （mammalian derived）

  • 产品型号：  100-21
  • 简单描述
  • Recombinant Human TGF-β1 (mammalian derived)重组人TGF-β1细胞因子，详细产品信息请
详细介绍

 

Recombinant Human TGF-β1 （mammalian derived）

Catalog Number:
100-21
Synonyms:
Transforming Growth Factor-β1, Differentiation inhibiting factor, Cartilage-inducing factor
Description:
The three mammalian isoforms of TGF-β, TGF-β1, β2, β3, signal through the same receptor and elicit similar biological responses. They are multifunctional cytokines that regulate cell proliferation, growth, differentiation and motility as well as synthesis and deposition of the extracellular matrix. They are involved in various physiological processes including embryogenesis, tissue remodeling and wound healing. They are secreted predominantly as latent complexes which are stored at the cell surface and in the extracellular matrix. The release of biologically active TGF-β isoform from a latent complex involves proteolytic processing of the complex and /or induction of conformational changes by proteins such as thrombospondin-1. TGF-β1 is the most abundant isoform secreted by almost every cell type. It was originally identified for its ability to induce phenotypic transformation of fibroblasts and recently it has been implicated in the formation of skin tumors. Human TGF-β1 is a 25.0 kDa protein with each subunit containing 112 amino acid residues, linked by a single disulfide bond.
AA Sequence:
ALDTNYCFSS TEKNCCVRQL YIDFRKDLGW KWIHEPKGYH ANFCLGPCPY IWSLDTQYSK VLALYNQHNP GASAAPCCVP QALEPLPIVY YVGRKPKVEQ LSNMIVRSCK CS
Source:
HEK293 cells
Purity:
Greater than 98% by SDS-PAGE gel and HPLC analyses.
Biological Activity:
The ED50 was determined by TGF-beta1’s ability to inhibit the mouse IL-4-dependent proliferation of mouse HT-2 cells is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 107 units/mg.
 
 
 
 
Cross Reactivity:
Chicken, Cow, Dog, Frog, Monkey, Mouse, Pig, Rabbit, Rat, Human, Mink, Donkey, N/A
Research Interests:
Peprotech重组人TGF-β1细胞因子Recombinant Human TGF-β1 （mammalian derived）100-21 Angiogenesis/ Cardiovascular Peprotech重组人TGF-β1细胞因子Recombinant Human TGF-β1 （mammalian derived）100-21 Apoptosis
Peprotech重组人TGF-β1细胞因子Recombinant Human TGF-β1 （mammalian derived）100-21 Cancer Peprotech重组人TGF-β1细胞因子Recombinant Human TGF-β1 （mammalian derived）100-21 Immune System
Peprotech重组人TGF-β1细胞因子Recombinant Human TGF-β1 （mammalian derived）100-21 Inflammation Peprotech重组人TGF-β1细胞因子Recombinant Human TGF-β1 （mammalian derived）100-21 Proliferation
Peprotech重组人TGF-β1细胞因子Recombinant Human TGF-β1 （mammalian derived）100-21 Stem Cells & Differentiation Peprotech重组人TGF-β1细胞因子Recombinant Human TGF-β1 （mammalian derived）100-21 TGF-β Superfamily
Peprotech重组人TGF-β1细胞因子Recombinant Human TGF-β1 （mammalian derived）100-21 Wound Healing Peprotech重组人TGF-β1细胞因子Recombinant Human TGF-β1 （mammalian derived）100-21 Allergy
 
 
Country of Origin:
USA

R&D Recombinant Human/mouse IL-17A重组人/小鼠白介17细胞因子RD 317-ILB-050

发表于由

R&D Recombinant Human/mouse IL-17A重组人/小鼠白介17细胞因子

  • 产品型号：  RD 317-ILB-050
  • 简单描述
  • 产品详细信息请
详细介绍

Activity Measured by its ability to induce IL6
secretion by NHDF human normal dermal fibroblasts.
The ED50 for this effect is typically 26
ng/mL.
Measured by its ability to induce IL6
secretion by NIH-3T3 mouse embryonic fibroblast cells. Yao, Z. et al. （1995） Immunity 3:811.
The ED50 for this effect is typically 1.57.5
ng/mL.
Endotoxin Level <1.0 EU per 1 μg of the protein by the LAL method.
Purity >97%, by SDSPAGE
under reducing conditions and visualized by silver stain.
Formulation Lyophilized from a 0.2 μm filtered solution in HCl. See Certificate of Analysis for details.
PREPARATION AND STORAGE
Reconstitution Reconstitute at 100 μg/mL in 4 mM HCI.
Shipping The product is shipped with polar packs. Upon receipt, store it immediay at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freezethaw
cycles.
l 12 months from date of receipt, 20
to 70
°C as supplied.
l 1 month, 2 to 8 °C under sterile conditions after reconstitution.
l 3 months, 20
to 70
°C under sterile conditions after reconstitution.
BACKGROUND
Interleukin 17 （also known as CTLA8）
is a T cellexpressed
pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of
herpesvirus Saimiri. cDNA clones encoding IL17
have been isolated from activated rat, mouse and human T cells. Human IL17
cDNA encodes a 155 amino acid （aa）
residue precursor protein with a 19 amino acid residue signal peptide that is cleaved to yield the 136 aa residue mature IL17
containing one potential Nlinked
glycosylation site. Both recombinant and natural IL17
have been shown to exist as disulfidelinked
homodimers. At the amino acid level, human IL17
shows 72% and
63% sequence identity with herpesvirus and rat IL17,
respectively. An IL17
specific mouse cell surface receptor （IL17
R） has been cloned. While the expression of
IL17
mRNA is restricted to activated T cells, the expression of mIL17
R mRNA has been detected in virtually all cells and tissues tested. IL17
exhibits multiple
biological activities on a variety of cells including the induction of IL6
and IL8
production in fibroblasts, the enhancement of surface expression of ICAM1
in
fibroblasts, activation of NFκB
and costimulation of T cell proliferation. The nonglycosylated,
E. coliexpressed
rhIL17
has been shown to be active on human as
well as mouse cell lines.
References:
1. Yao, Z. et al. （1995） J. Immunol. 155:5483.
2. Yao, Z. et al. （1995） Immunity 3:811.
3. Rouvier, E. et al. （1993） J. Immunol. 150:5445

 

R&D供应商Recombinant Rat FGF basic 重组大鼠碱性成纤维细胞生长因子RD 3339-FB-025

发表于由

R&D供应商Recombinant Rat FGF basic 重组大鼠碱性成纤维细胞生长因子

  • 产品型号：  RD 3339-FB-025
  • 简单描述
  • Recombinant Rat FGF basic ，Recombinant Human FGF basic ，Recombinant Human KGF/FGF-7，Recombinant Human/Mouse FGF-8b
详细介绍

DESCRIPTION
Source E. coliderived
Ala11Ser154
Accession # P13109
Nterminal
Sequence
Analysis
Ala11
Predicted Molecular
Mass
16.2 kDa
SPECIFICATIONS
Activity Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells. Rizzino, A. et al. （1988） Cancer Res. 48:4266; Thomas, K. et
al. （1987） Methods Enzymol. 147:120.
The ED50 for this effect is typically 0.31.8
ng/mL.
Endotoxin Level <1.0 EU per 1 μg of the protein by the LAL method.
Purity >95%, by SDSPAGE
under reducing conditions and visualized by silver stain.
Formulation Lyophilized from a 0.2 μm filtered solution in MOPS, Na2SO4, EDTA and DTT with BSA as a carrier protein. See Certificate of Analysis for
details.
PREPARATION AND STORAGE
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediay at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freezethaw
cycles.
l 12 months from date of receipt, 20
to 70
°C as supplied.
l 1 month, 2 to 8 °C under sterile conditions after reconstitution.
l 3 months, 20
to 70
°C under sterile conditions after reconstitution.
BACKGROUND
FGF basic （FGF2,
HBGF2）
is one of at least 22 mitogenic proteins of the FGF family, which show 35 60%
amino acid conservation （1 3）.
Unlike other FGFs,
FGF acidic and basic lack signal peptides and are secreted by an alternate pathway. Storage pools within the cell or on cell surface heparan sulfate proteoglycans
（HSPG） are likely. The predicted 18 kDa FGF basic isoform can be located in both the cytoplasm and the nucleus and is presumed to be the form secreted （4）.
Transcription from alternate start sites produces 21 23
kDa forms found only in the nucleus. High and low molecular weight human FGF basic targets the expression
of different genes when expressed in NIH3T3
cells （5）. The 18 kDa rat sequence has 98% amino acid （aa） identity with mouse, and 96 97%
aa identity with human,
bovine and sheep FGF basic （6, 7）. Autocrine, intracrine and paracrine actions of FGF basic have been identified. Binding of FGF to heparin or cell surface HSPG is
necessary for binding, dimerization and activation of tyrosine kinase FGF receptors. FGF basic binds other proteins, polysaccharides and lipids with lower affinity （3）.
Expression of FGF basic is nearly ubiquitous but disruption of the mouse FGF basic gene gives a relatively mild phenotype, suggesting compensation by other FGF
family members. FGF basic modulates such normal processes as angiogenesis, wound healing and tissue repair, embryonic development and differentiation, neuronal
function and neural degeneration. Transgenic overexpression of FGF basic results in excessive proliferation and angiogenesis reminiscent of a variety of pathological
conditions （1 3）.
References:
1. Coulier, F. et al. （1997） J. Mol. Evol. 44:43.
2. Fernig, D. et al. （1994） Prog. Growth Factor Res. 5:353.
3. Presta, M. et al. （2005） Cytokine Growth Factor Rev. 16:159.
4. Claus, P. et al. （2003） J. Biol. Chem. 278:479.
5. Quarto, N. et al. （2005） Gene 356:49.
6. Tsuneto, M. et al. （2005） Biochem. Biophys. Res. Commun. 335:1239.
7. Shimasaki, S. et al. （1988） Biochem. Biophys. Res. Commun. 157:256.
Recombinant Rat FGF basic
Catalog Number: 3339-FB
 

 

R&D代理Recombinant Rat EGF重组鼠表皮生长因子RD供应*3214-EG-100

发表于由

R&D代理Recombinant Rat EGF重组鼠表皮生长因子RD供应*

  • 产品型号：  3214-EG-100
  • 简单描述
  • 此类产品还有人的表皮细胞生长因子，上海利民实业有限公司是华东区RD代理，咨询产品 纤细说明书见详细介绍
详细介绍

DESCRIPTION
Source E. coliderived
Asn974Arg1026,
with an Nterminal
Met
Accession # P07522
Nterminal
Sequence
Analysis
Met
Predicted Molecular
Mass
6.3 kDa
SPECIFICATIONS
Activity Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. Rubin, J.S. et al. （1991） Proc. Natl. Acad. Sci. USA
88:415.
The ED50 for this effect is typically 1060
pg/mL.
Endotoxin Level <1.0 EU per 1 μg of the protein by the LAL method.
Purity >97%, by SDSPAGE
under reducing conditions and visualized by silver stain.
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein. See Certificate of Analysis for details.
PREPARATION AND STORAGE
Reconstitution Reconstitute at 100 μg/mL in sterile 10 mM Acetic Acid containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediay at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freezethaw
cycles.
l 12 months from date of receipt, 20
to 70
°C as supplied.
l 1 month, 2 to 8 °C under sterile conditions after reconstitution.
l 3 months, 20
to 70
°C under sterile conditions after reconstitution.
BACKGROUND
Epidermal growth factor （EGF） is the founding member of the EGF family that also includes TGFα,
amphiregulin （AR）, betacellulin （BTC）, epiregulin （EPR）, heparinbinding
EGFlike
growth factor（HBEGF）,
epigen, and the neuregulins （NRG）1
through 6
（1）. Members of the EGF family share a structural motif, the EGFlike
domain, which is characterized by three intramolecular disulfide bonds that are formed by six similarly spaced conserved cysteine residues （2）. All EGF family
members are synthesized as type I transmembrane precursor proteins that may contain several EGF domains in the extracellular region. The mature proteins are
released from the cell surface by regulated proteolysis （1）. The 1133 amino acid （aa） rat EGF precursor contains nine EGF domains and nine LDLR class B repeats.
The mature protein consists of 53 aa and is generated by proteolytic excision of the EGF domain proximal to the transmembrane region （3）. Mature rat EGF shares
70% and 77% aa sequence identity with mature human and mouse EGF, respectively. EGF is present in various body fluids, including blood, milk, urine, saliva,
seminal fluid, pancreatic juice, cerebrospinal fluid, and amniotic fluid （4）. Four ErbB （HER） family receptor tyrosine kinases including EGFR/ErbB1, ErbB2, ErbB3 and
ErbB4, mediate responses to EGF family members （5）. These receptors undergo a complex pattern of ligand induced homoor
heterodimerization
to transduce EGF
family signals （6, 7）. EGF binds ErbB1 and depending on the context, induces the formation of homodimers or heterodimers containing ErbB2. Dimerization results in
autophosphorylation of the receptor at specific tyrosine residues to create docking sites for a variety of signaling molecules （5, 8）. Biological activities ascribed to
EGF include epithelial development, angiogenesis, inhibition of gastric acid secretion, fibroblast proliferation, and colony formation of epidermal cells in culture.
References:
1. Harris, R.C. et al. （2003） Exp. Cell Res. 284:2.
2. Carpenter, G. and Cohen, S. （1990） J. Biol. Chem. 265:7709.
3. Saggi, S.J. et al. （1992） DNA Cell Biol. 11:481.
4. Carpenter, G. and Zendegui, J.G. （1986） Exp. Cell Res. 164:1.
5. Jorissen, R.N. et al. （2003） Exp. Cell Res. 284:31.
6. Gamett, D.C. et al. （1997） J. Biol. Chem. 272:12052.
7. Qian, X. et al. （1994） Proc. Natl. Acad. Sci. 91:1500.
8. Qian, X. et al. （1999） J. Biol. Chem. 274:574.
Recombinant Rat EGF
Catalog Number: 3214-EG
www.

M-CSF的重组小鼠细胞因子Recombinant Mouse M-CSFRD 416-ml-010

发表于由

M-CSF的重组小鼠细胞因子Recombinant Mouse M-CSF

  • 产品型号：  RD 416-ml-010
  • 简单描述
  • M-CSF的重组小鼠细胞因子Recombinant Mouse M-CSF也被称为CSF1细胞因子，巨噬细胞的存活，增殖和分化（1）主要监管机构纤细说明书在产品详细介绍中
详细介绍

M-CSF的重组小鼠细胞因子Recombinant Mouse M-CSF

DESCRIPTION
Source E. coliderived
Lys33Glu262,
with an Nterminal
Met
Accession # Q3U4F9
Nterminal
Sequence
Analysis
Met
Structure / Form Disulfidelinked
homodimer
Predicted Molecular
Mass
26 kDa （monomer）
SPECIFICATIONS
SDSPAGE
29 kDa, reducing conditions
Activity Measured in a cell proliferation assay using M-NFS-60 mouse myelogenous leukemia lymphoblast cells. Halenbeck, R. et al. （1989）
Biotechnology 7:710.
The ED50 for this effect is typically 0.5-3 ng/mL.
Endotoxin Level <1.0 EU per 1 μg of the protein by the LAL method.
Purity >97%, by SDSPAGE
under reducing conditions and visualized by silver stain.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. See Certificate of Analysis for details.
PREPARATION AND STORAGE
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediay at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freezethaw
cycles.
l 12 months from date of receipt, 20
to 70
°C as supplied.
l 1 month, 2 to 8 °C under sterile conditions after reconstitution.
l 3 months, 20
to 70
°C under sterile conditions after reconstitution.
BACKGROUND
MCSF,
also known as CSF1,
is a fourαhelicalbundle
cytokine that is the primary regulator of macrophage survival, proliferation and differentiation （1 3）.
MCSF
is also essential for the survival and proliferation of osteoclast progenitors （1, 4）. MCSF
also primes and enhances macrophage killing of tumor cells and
microorganisms, regulates the release of cytokines and other inflammatory modulators from macrophages, and stimulates pinocytosis （2, 3）. MCSF
increases during
pregnancy to support implantation and growth of the decidua and placenta （5）. Sources of MCSF
include fibroblasts, activated macrophages, endometrial secretory
epithelium, bone marrow stromal cells and activated endothelial cells （1 5）.
The MCSF
receptor （cfms）
transduces its pleotropic effects and mediates its
endocytosis. MCSF
mRNAs of various sizes occur （3 9）.
Full length mouse MCSF
transcripts encode a 520 amino acid （aa） type I transmembrane （TM） protein with
a 462 aa extracellular region, a 21 aa TM domain, and a 37 aa cytoplasmic tail that forms a 140 kDa covalent dimer. Differential processing produces two
proteolytically cleaved, secreted dimers. One is an Nand
Oglycosylated
86 kDa dimer, while the other is modified by both glycosylation and chondroitinsulfate
proteoglycan （PG） to generate a 200 kDa subunit. Although PGmodified
MCSF
can circulate, it may be immobilized by attachment to type V collagen （8）. Shorter
transcripts encode M CSF
that lacks cleavage and PG sites and produces an Nglycosylated
68 kDa TM dimer and a slowly produced 44 kDa secreted dimer （7）.
Although forms may vary in activity and halflife,
all contain the Nterminal
150 aa portion that is necessary and sufficient for interaction with the M CSF
receptor
（10, 11）. The first 229 aa of mature mouse MCSF
shares 87%, 83%, 82% and 81% aa identity with corresponding regions of rat, dog, cow and human MCSF,
respectively （12, 13）. Human MCSF
is active in the mouse, but mouse MCSF
is reported to be speciesspecific.
M-CSF的重组小鼠细胞因子Recombinant Mouse M-CSF

References:
1. Pixley, F.J. and E.R. Stanley （2004） Trends Cell Biol. 14:628.
2. Chitu, V. and E.R. Stanley （2006） Curr. Opin. Immunol. 18:39.
3. Fixe, P. and V. Praloran （1997） Eur. Cytokine Netw. 8:125.
4. Ryan, G.R. et al. （2001） Blood 98:74.
5. Makrigiannakis, A. et al. （2006） Trends Endocrinol. Metab. 17:178.
6. Nandi, S. et al. （2006） Blood 107:786.
7. Rettenmier, C.W. and M.F. Roussel （1988） Mol. Cell Biol. 8:5026.
8. Suzu, S. et al. （1992） J. Biol. Chem. 267:16812.
9. Manos, M.M. （1988） Mol. Cell. Biol. 8:5035.
10. Koths, K. （1997） Mol. Reprod. Dev. 46:31.
11. Jang, MH.
et al. （2006） J. Immunol. 177:4055.
12. DeLamarter, J.F. et al. （1987） Nucleic Acids Res. 15:2389.
13. Ladner, M.B. et al. （1988） Proc. Natl. Acad. Sci. USA 85:6706.

 

RD Recombinant human IL-17A人白介17因子314-ml-025

发表于由

RD Recombinant human IL-17A人白介17因子

  • 产品型号：  314-ml-025
  • 简单描述
  • RD Recombinant human IL-17A人白介17因子Source E. coliderived此产品还有小鼠IL-17A
详细介绍

RD Recombinant human IL-17A人白介17因子

DESCRIPTION
Source E. coliderived
Thr22Ala158
Accession # Q62386.1
Nterminal
Sequence
Analysis
Thr22
Structure / Form Disulfidelinked
homodimer
Predicted Molecular
Mass
15.5 kDa （monomer）
SPECIFICATIONS
Activity Measured by its ability to induce IL6
secretion by NIH-3T3 mouse embryonic fibroblast cells. Yao, Z. et al. （1995） Immunity 3:811.
The ED50 for this effect is typically 0.251.25
ng/mL.
Endotoxin Level <1.0 EU per 1 μg of the protein by the LAL method.
Purity >97%, by SDSPAGE
under reducing conditions and visualized by silver stain.
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein. See Certificate of Analysis for details.
PREPARATION AND STORAGE
Reconstitution Reconstitute at 25 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediay at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freezethaw
cycles.
l 12 months from date of receipt, 20
to 70
°C as supplied.
l 1 month, 2 to 8 °C under sterile conditions after reconstitution.
l 3 months, 20
to 70
°C under sterile conditions after reconstitution.
BACKGROUND
Interleukin 17 （also known as CTLA8）
is a T cellexpressed
pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of
herpesvirus Saimiri. cDNA clones encoding IL17
have been isolated from activated rat, mouse and human T cells. Mouse IL17
cDNA encodes a 158 amino acid （aa）
residue precursor protein with a 21 amino acid residue signal peptide that is cleaved to yield the 137 aa residue mature IL17.
Both recombinant and natural IL17
have
been shown to exist as disulfide linked homodimers. At the amino acid level, mIL17
shows 57% and 87% sequence identity with herpesvirus and rat IL17,
respectively. An IL17
specific mouse cell surface receptor （IL17
R） has been cloned. While the expression of IL17
mRNA is restricted to activated alpha beta
TCR+CD4CD8T
cells, the expression of mIL17
R mRNA has been detected in virtually all cells and tissues tested. IL17
exhibits multiple biological activities on a
variety of cells including: the induction of IL6
and IL8
production in fibroblasts? the enhancement of surface expression of ICAM1
in fibroblasts? activation of NFκB
and costimulation of T cell proliferation.
References:
1. Kennedy, J. et al., （1996） J. Interferon Cytokine Res. 16:611.
2. Yao, Z. et al., （1995） J. Immunol. 155:5483.
3. Yao, Z. et al., （1995） Immunity 3:811.
4. Rouvier, E. et al., （1993） J. Immunol. 150:5445.

RD Recombinant human IL-17A人白介17因子 详细产品信息可和选购

 

Recombinant Mouse M-CSF巨噬细胞集落刺激因子R&D 416-ml

发表于由

Recombinant Mouse M-CSF巨噬细胞集落刺激因子

  • 产品型号：  R&D 416-ml
  • 简单描述
  • Recombinant Mouse M-CSF巨噬细胞集落刺激因子上海金畔代理R&D产品，美国*Recombinant Mouse M-CSF，Recombinant human M-CSF，规格分别是10ug和50ug 咨询还有人的Human M-CSF
详细介绍

Recombinant Mouse M-CSF巨噬细胞集落刺激因子

MCSF,
also known as CSF1,
is a fourαhelicalbundle
cytokine that is the primary regulator of macrophage survival, proliferation and differentiation （1 3）.
MCSF
is also essential for the survival and proliferation of osteoclast progenitors （1, 4）. MCSF
also primes and enhances macrophage killing of tumor cells and
microorganisms, regulates the release of cytokines and other inflammatory modulators from macrophages, and stimulates pinocytosis （2, 3）. MCSF
increases during
pregnancy to support implantation and growth of the decidua and placenta （5）. Sources of MCSF
include fibroblasts, activated macrophages, endometrial secretory
epithelium, bone marrow stromal cells and activated endothelial cells （1 5）.
The MCSF
receptor （cfms）
transduces its pleotropic effects and mediates its
endocytosis. MCSF
mRNAs of various sizes occur （3 9）.
Full length mouse MCSF
transcripts encode a 520 amino acid （aa） type I transmembrane （TM） protein with
a 462 aa extracellular region, a 21 aa TM domain, and a 37 aa cytoplasmic tail that forms a 140 kDa covalent dimer. Differential processing produces two
proteolytically cleaved, secreted dimers. One is an Nand
Oglycosylated
86 kDa dimer, while the other is modified by both glycosylation and chondroitinsulfate
proteoglycan （PG） to generate a 200 kDa subunit. Although PGmodified
MCSF
can circulate, it may be immobilized by attachment to type V collagen （8）. Shorter
transcripts encode M CSF
that lacks cleavage and PG sites and produces an Nglycosylated
68 kDa TM dimer and a slowly produced 44 kDa secreted dimer （7）.
Although forms may vary in activity and halflife,
all contain the Nterminal
150 aa portion that is necessary and sufficient for interaction with the M CSF
receptor
（10, 11）. The first 229 aa of mature mouse MCSF
shares 87%, 83%, 82% and 81% aa identity with corresponding regions of rat, dog, cow and human MCSF,
respectively （12, 13）. Human MCSF
is active in the mouse, but mouse MCSF
is reported to be speciesspecific

Recombinant Mouse M-CSF巨噬细胞集落刺激因子

 

上海金畔R&D一级代理咨询 

重组Cas9蛋白质 (3 μg/μl)酶试剂盒Takara

发表于由

重组Cas9蛋白质 (3 μg/μl)
品牌 Code No. 产品名称 包装量 价格（元） 说明书 数量
Clontech 632640 Guide-it Recombinant Cas9 (3 μg/μl) 3 × 100 μg ￥6,845 重组Cas9蛋白质 (3 μg/μl) 重组Cas9蛋白质 (3 μg/μl) 重组Cas9蛋白质 (3 μg/μl)
Clontech 632641 Guide-it Recombinant Cas9 (3 μg/μl) 100 μg ￥2,701 重组Cas9蛋白质 (3 μg/μl) 重组Cas9蛋白质 (3 μg/μl) 重组Cas9蛋白质 (3 μg/μl)
收藏产品 加入购物车

无标题文档

 
Guide-it Recombinant Cas9 (Electroporation Ready)（Code No. 632641/632640）产品名称变更为Guide-it Recombinant Cas9 (3 μg/μl) ，产品内容没有发生变化。（2021年5月）
 
 
          基因敲除    
          ↗ 重组Cas9蛋白质 (3 μg/μl) → 重组Cas9蛋白质 (3 μg/μl) → 重组Cas9蛋白质 (3 μg/μl)
重组Cas9蛋白质 (3 μg/μl) → 重组Cas9蛋白质 (3 μg/μl) → 重组Cas9蛋白质 (3 μg/μl)            
  基因敲入        
          ↘ 重组Cas9蛋白质 (3 μg/μl)        
 
 
降低脱靶效应，可用于in vivo基因编辑实验
Guide-it™ Recombinant Cas9 (3 μg/μl)
· 适用于电穿孔方法的低甘油含量、高浓度的Cas9蛋白质溶液
· 与质粒导入系统相比较，脱靶效应受到明显抑制
· NLS（核定位信号）有助于实现快速高效突变导入
· 与Guide-it Complete sgRNA Screening System组合使用实现高效率的基因编辑
 
■ 产品说明：
Guide-it Recombinant Cas9（3 μg/μl）是来源于野生型酿脓链球菌Streptococcus pyogenes，适用于CRISPR/Cas9基因编辑实验的重组Cas9蛋白质溶液。
高浓度Cas9蛋白质溶液中甘油浓度比其他公司低，特别适用于电穿孔导入方法。与借助质粒导入Cas9基因序列方法相比较，避免了残留Cas9基因的持续表达，有效抑制了脱靶效应。
无需经过蛋白质转录·翻译·表达过程，C末端的NLS（核定位信号）可以实现快速有效的突变导入。
Cas9导入细胞首先需要形成Cas9-sgRNA核糖核蛋白复合物，sgRNA制备推荐使用Guide-it sgRNA In VitroTranscription Kit（Code No. 632635）或者Guide-it Complete sgRNA Screening System（Code No. 632636）。
 
■ 实验例：
重组Cas9蛋白质 (3 μg/μl)
图1. AAVS1或者CXCR4基因同源重组修复（HDR）效率
Guide-it Recombinant Cas9（3 μg/μl）和修复模板复合物通过电穿孔的方式导入至CD34＋造血干细胞中检测同源重组修复（HDR）效率。将包含Hind III酶切位点的修复模板插入至AAVS1 基因中，包含Hind III和BamH I酶切位点的修复模板插入至CXCR4基因中。之后通过对细胞内靶基因进行PCR扩增和限制酶剪切分析，确认突变导入效率。基因编辑效率标注在凝胶阳性孔处。在基因编辑前野生型CXCR4基因已包含1处BamH I位点，因此BamH I剪切CXCR4基因后额外多显示一条条带。
 
重组Cas9蛋白质 (3 μg/μl)
图2. 不同公司Cas9产品在hiPS細胞中基因敲除效率比较
剪切后电泳条带的强弱可以半定量估计发生基因编辑细胞的百分数。Guide-it Recombinant Cas9（3 μg/μl）与Guide-it sgRNA In Vitro Transcription Kit制备的sgRNA相结合，通过电穿孔的方法导入Cellartis hiPSC-18细胞，采用Guide-it Mutation Detection Kit（Code No. 631448）确认突变导入效率，％即表示基因编辑效率。如图所示，Guide-it Recombinant Cas9（3 μg/μl）在多数靶基因位点均显示有效的基因编辑。
  * 比较结果来自于Takara Bio USA, Inc.
 
 
■ 浓度
3 μg/μl
 
■ 保存
－70℃
（注意）避免反复冻融。首次溶解之后，建议按照使用量分装保存。
 
■ 所需要的其它产品（主要产品）
· 电穿孔装置：
Neon Transfection System（Thermo Fisher Scientific）或者 4-D Nucleofector System（Lonza）
*各装置的导入条件请阅读使用说明书。

· sgRNA制备及筛选
Guide-it sgRNA In Vitro Transcription Kit（Code No. 632635）：用于sgRNA制备及纯化
Guide-it Complete sgRNA Screening System（Code No. 632636）：用于确认制备的sgRNA的有效性
 
 
产品详情请点击：重组Cas9蛋白质 (3 μg/μl)
 
 
■ 关联资料
相关资料：
点击图片下载		 重组Cas9蛋白质 (3 μg/μl) 重组Cas9蛋白质 (3 μg/μl)
 
 

页面更新：2021-07-19 12:52:26

Recombinant RNase Inhibitor酶试剂盒Takara

发表于由

Recombinant RNase Inhibitor
品牌 Code No. 产品名称 包装量 价格（元） 说明书 数量
Takara 2313Q Recombinant RNase Inhibitor 500 U ￥95 Recombinant RNase Inhibitor Recombinant RNase Inhibitor Recombinant RNase Inhibitor
Takara 2313A Recombinant RNase Inhibitor 5,000 U ￥471
￥400 		Recombinant RNase Inhibitor Recombinant RNase Inhibitor Recombinant RNase Inhibitor
Takara 2313B (A × 5) Recombinant RNase Inhibitor 5,000 U × 5 ￥2,113
￥1,796 		Recombinant RNase Inhibitor Recombinant RNase Inhibitor Recombinant RNase Inhibitor

*红字为促销价格，促销时间: 2022年9月1日-2022年10月31日

收藏产品 加入购物车
 
■ 制品内容 (Code No.: 2313A)
Recombinant RNase Inhibitor (40 U/μl) 5,000 U
 
■ 制品说明
本制品是通过亲和色谱等方法从大肠杆菌中纯化的猪肝RNA酶抑制剂得到的重组体。该酶的特征与猪肝和人胎盘来源的此类酶相似，与RNase A形成1：1复合体，抑制RNase活性。该反应是可逆的，通过尿素及巯基类试剂能够解离复合体，使RNase复活而抑制剂不可逆失活。使用时可直接加入含有RNA的反应液中。本品属蛋白质性质，与其它竞争性抑制剂 (核酸类、无机磷酸类) 不同，可以很容易地通过苯酚处理将其从反应体系中除去。但不能抑制反转录酶的RNase H的活性。本品具有与猪肝和人胎盘来源的酶相同的应用。
 
■ 保存
-20℃。
 
■ 起源
E. coli containing a plasmid that carries the porcine liver RNase Inhibitor gene.
 
■ 活性定义
抑制5 ng RNase A活性的50%所需要的酶量定义为1个活性单位 (U)。(抑制活性通过抑制RNase A水解Cyclic2’,3’-CMP的能力确定) 。
 
■ 性质
分子量：约52,000
等电点：5.0
最适pH：pH6～9
最适温度：25℃～50℃
激活剂：还原剂DTT（贮存Buffer中至少含有1 mM DTT，以保护处于游离状态的-SH）。
稳定性：起泡或剧烈搅拌 (Vortex等) 可能会引起失活。65℃热处理15分钟可使其失活。
 
■ 使用注意
1. 抑制RNase活性的pH值范围较广，在pH7～8时表现最适范围。
2. RNase Inhibitor发挥活性作用需要至少1 mM的DTT。
 
■ 用途
1. cDNA合成反应 (Ribonuclease Inhibitor, 反应量0.5 U/μl) 。
2. 体外翻译 (Ribonuclease Inhibitor, 反应量1 U/μl) 。
3. 体外无细胞系统转录 (Ribonuclease Inhibitor, 反应量20 U/μl) 。
4. SP6或T7 RNA聚合酶的体外转录 (Ribonuclease Inhibitor,反应量1 U/μl) 。
5. 多核糖体分离(RNase Inhibitor，反应量1 U/μl)。
 
 

页面更新：2020-07-27 08:49:33

Recombinant RNase Inhibitor, GPR酶试剂盒Takara

发表于由

Recombinant RNase Inhibitor, GPR
品牌 Code No. 产品名称 包装量 价格（元） 说明书 数量
Takara 2314A Recombinant RNase Inhibitor, GPR 5,000 U ￥994 Recombinant RNase Inhibitor, GPR Recombinant RNase Inhibitor, GPR Recombinant RNase Inhibitor, GPR
Takara 2314B (A × 5) Recombinant RNase Inhibitor, GPR 5,000 U × 5 ￥4,723 Recombinant RNase Inhibitor, GPR Recombinant RNase Inhibitor, GPR Recombinant RNase Inhibitor, GPR
收藏产品 加入购物车
 
■ 制品内容 (Code No.: 2314A)
Recombinant RNase Inhibitor，GPR (40 U/μl) 5,000 U
 
■ 制品说明
Recombinant RNase Inhibitor, GPR是注册的general purpose reagent (GPR)级别试剂，可用于常规实验应用，包括分子诊断的开发和检测，该产品是通过亲和层析从E. coli中分离的重组蛋白质。该产品与猪肝或人胎盘来源的RNase抑制剂性质类似，可与RNase A形成1:1复合物以抑制RNase活性。该反应是可逆的，使用尿素或巯基试剂溶解复合物可使抑制剂不可逆的失活而恢复RNA酶活性。RNase抑制剂可直接加入到含RNA的反应液中。而且，与其他非蛋白质的竞争性抑制剂（如核酸或无机磷酸盐）不同，该抑制剂可方便地通过苯酚抽提从体系中去除。该产品不能抑制反转录酶的RNase H活性，与人胎盘或猪肝来源的RNase抑制剂用途相同。
 
■ 保存
-20℃。
 
■ 起源
E. coli containing a plasmid that carries the porcine RNase Inhibitor gene.
 
 
 

页面更新：2017-05-19 13:39:42

Recombinant DNase I (RNase-free)酶试剂盒Takara

发表于由

Recombinant DNase I (RNase-free)
品牌 Code No. 产品名称 包装量 价格（元） 说明书 数量
Takara 2270A Recombinant DNase I (RNase-free) 1,000 U ￥501 Recombinant DNase I (RNase-free) Recombinant DNase I (RNase-free) Recombinant DNase I (RNase-free)
Takara 2270B (A × 5) Recombinant DNase I (RNase-free) 1,000 U × 5 ￥2,253 Recombinant DNase I (RNase-free) Recombinant DNase I (RNase-free) Recombinant DNase I (RNase-free)
收藏产品 加入购物车
 
■ 制品内容 (Code No.: 2270A)
Recombinant DNase I (RNase-free；5 U/μl) 1,000 U
10X DNase I Buffer 1 ml
 
■ 制品说明
本酶是将单链或双链DNA同等程度的随机分解，生成具有5’-P末端寡核苷酸的脱氧核糖核酸内切酶。
由于本酶已除去了蛋白酶和核酸酶，从而提高了在pH中性区域的稳定性，适用于RNA的制备。
 
■ 保存
-20℃。
 
■ 起源
Recombinant enzyme derived from non-animal host.
 
■ 活性定义
以小牛胸腺DNA为底物，在25℃、pH5.0的条件下，1分钟内使反应液的260 nm吸光度增加0.001所需要的酶量定义为1个活性单位 (Kunitz Unit)。
 
■ 用途
1. 用T7或SP6 RNA Polymerase进行体外转录 (In vitro transcription) 时，合成RNA后，不必更换Buffer，使用Recombinant DNase I (RNase-free) 即可将模板DNA降解。
2. 与DNA Polymerase I (Code No. : 2130) 一起用于切口平移。
3. 在Mn2+存在的条件下，为鸟枪法测序 (Shotgun sequencing) 制作DNA文库。
4. 用于足迹法 (Foot-printing) 分析DNA-蛋白质相互作用。
5. 在进行RT-PCR反应前，对基因组DNA进行降解。
 
■ 使用注意
本酶已除去蛋白酶，在最适中性pH范围内稳定。反应时需要二价金属离子参与，Mg2+存在时，能随机使双链DNA产生切口；Mn2+存在时，双链DNA的两条链被分解为片段。加入EDTA可使酶可逆性失活，75℃加热10分钟则不可逆性失活。
 
 

页面更新：2019-03-07 16:14:11

重组Cas9蛋白质 (3 μg/μl)酶试剂盒Takara

发表于由

重组Cas9蛋白质 (3 μg/μl)
品牌 Code No. 产品名称 包装量 价格（元） 说明书 数量
Clontech 632640 Guide-it Recombinant Cas9 (3 μg/μ) 3 × 100 μg ￥6,845 重组Cas9蛋白质 (3 μg/μl) 重组Cas9蛋白质 (3 μg/μl) 重组Cas9蛋白质 (3 μg/μl)
Clontech 632641 Guide-it Recombinant Cas9 (3 μg/μ) 100 μg ￥2,701 重组Cas9蛋白质 (3 μg/μl) 重组Cas9蛋白质 (3 μg/μl) 重组Cas9蛋白质 (3 μg/μl)
收藏产品 加入购物车

无标题文档

 
Guide-it Recombinant Cas9 (Electroporation Ready)（Code No. 632641/632640）产品名称变更为Guide-it Recombinant Cas9 (3 μg/μl) ，产品内容没有发生变化。（2021年5月）
 
 
          基因敲除    
          ↗ 重组Cas9蛋白质 (3 μg/μl) → 重组Cas9蛋白质 (3 μg/μl) → 重组Cas9蛋白质 (3 μg/μl)
重组Cas9蛋白质 (3 μg/μl) → 重组Cas9蛋白质 (3 μg/μl) → 重组Cas9蛋白质 (3 μg/μl)            
  基因敲入        
          ↘ 重组Cas9蛋白质 (3 μg/μl)        
 
 
降低脱靶效应，可用于in vivo基因编辑实验
Guide-it™ Recombinant Cas9 (3 μg/μl)
· 适用于电穿孔方法的低甘油含量、高浓度的Cas9蛋白质溶液
· 与质粒导入系统相比较，脱靶效应受到明显抑制
· NLS（核定位信号）有助于实现快速高效突变导入
· 与Guide-it Complete sgRNA Screening System组合使用实现高效率的基因编辑
 
■ 产品说明：
Guide-it Recombinant Cas9（3 μg/μl）是来源于野生型酿脓链球菌Streptococcus pyogenes，适用于CRISPR/Cas9基因编辑实验的重组Cas9蛋白质溶液。
高浓度Cas9蛋白质溶液中甘油浓度比其他公司低，特别适用于电穿孔导入方法。与借助质粒导入Cas9基因序列方法相比较，避免了残留Cas9基因的持续表达，有效抑制了脱靶效应。
无需经过蛋白质转录·翻译·表达过程，C末端的NLS（核定位信号）可以实现快速有效的突变导入。
Cas9导入细胞首先需要形成Cas9-sgRNA核糖核蛋白复合物，sgRNA制备推荐使用Guide-it sgRNA In VitroTranscription Kit（Code No. 632635）或者Guide-it Complete sgRNA Screening System（Code No. 632636）。
 
■ 实验例：
重组Cas9蛋白质 (3 μg/μl)
图1. AAVS1或者CXCR4基因同源重组修复（HDR）效率
Guide-it Recombinant Cas9（3 μg/μl）和修复模板复合物通过电穿孔的方式导入至CD34＋造血干细胞中检测同源重组修复（HDR）效率。将包含Hind III酶切位点的修复模板插入至AAVS1 基因中，包含Hind III和BamH I酶切位点的修复模板插入至CXCR4基因中。之后通过对细胞内靶基因进行PCR扩增和限制酶剪切分析，确认突变导入效率。基因编辑效率标注在凝胶阳性孔处。在基因编辑前野生型CXCR4基因已包含1处BamH I位点，因此BamH I剪切CXCR4基因后额外多显示一条条带。
 
重组Cas9蛋白质 (3 μg/μl)
图2. 不同公司Cas9产品在hiPS細胞中基因敲除效率比较
剪切后电泳条带的强弱可以半定量估计发生基因编辑细胞的百分数。Guide-it Recombinant Cas9（3 μg/μl）与Guide-it sgRNA In Vitro Transcription Kit制备的sgRNA相结合，通过电穿孔的方法导入Cellartis hiPSC-18细胞，采用Guide-it Mutation Detection Kit（Code No. 631448）确认突变导入效率，％即表示基因编辑效率。如图所示，Guide-it Recombinant Cas9（3 μg/μl）在多数靶基因位点均显示有效的基因编辑。
  * 比较结果来自于Takara Bio USA, Inc.
 
 
■ 浓度
3 μg/μl
 
■ 保存
－70℃
（注意）避免反复冻融。首次溶解之后，建议按照使用量分装保存。
 
■ 所需要的其它产品（主要产品）
· 电穿孔装置：
Neon Transfection System（Thermo Fisher Scientific）或者 4-D Nucleofector System（Lonza）
*各装置的导入条件请阅读使用说明书。

· sgRNA制备及筛选
Guide-it sgRNA In Vitro Transcription Kit（Code No. 632635）：用于sgRNA制备及纯化
Guide-it Complete sgRNA Screening System（Code No. 632636）：用于确认制备的sgRNA的有效性
 
 
产品详情请点击：重组Cas9蛋白质 (3 μg/μl)
 
 
■ 关联资料
相关资料：
点击图片下载		 重组Cas9蛋白质 (3 μg/μl) 重组Cas9蛋白质 (3 μg/μl)
 
 

页面更新：2021-07-20 09:32:43