Takara反转录试剂RT reagent Kit （Perfect Real Time）本制品是Real Time RT-PCR用的理想反转录反应试剂。使用具有较强延伸能力的PrimeScript RTase可以在较短时间内高效合成Real Time PCR用cDNA。操作简单，适合进行高通量分析货号：RR037A体系：20ul
详细介绍
Takara反转录试剂RT reagent Kit (Perfect Real Time)
试剂盒成分：
5×PrimeScript Buffer (for Real Time)*1
400 μl
PrimeScript RT Enzyme Mix I*2
100 μl
Oligo dT Primer (50 μM)
100 μl
Random 6 mers (100 μM)
400 μl
RNase Free dH2O
1 ml
EASY Dilution (for Real Time PCR)*3
1 ml
Takara反转录试剂RT reagent Kit (Perfect Real Time)
本制品是Real Time RT-PCR用的理想反转录反应试剂。使用具有较强延伸能力的PrimeScript RTase可以在较短时间内高效合成Real Time PCR用cDNA。操作简单，适合进行高通量分析。进行2 Step Real Time RT-PCR反应时，与SYBR® Premix Ex Taq™ II (Tli RNaseH Plus) (Code No. RR820Q/A/B) 、SYBR Fast qPCR Mix (Code No. RR430S/A/B) ， SYBR Premix Ex Taq (Tli RNaseH Plus) (Code No. RR420Q/A/B) 、 或Probe qPCR Mix (Code No. RR391S/A/B) 试剂配合使用，能进行高性能的基因表达分析。 本制品提供了SYBR Green分析法和探针分析法各自适合的反应体系，可以根据分析方法选择体系。
Takara反转录试剂RT reagent Kit (Perfect Real Time)
1. 可以快速、高效合成Real Time PCR用cDNA，是进行2 Step Real Time RT-PCR反应的理想试剂。 2. 含有Oligo dT Primer和Random 6 mers两种反转录引物，可根据实际情况区别使用。反转录反应可以使用Random 6 mers或Oligo dT Primer，也可以Oligo dT Primer和Random 6 mers同时使用。只扩增一种目的基因时，也可以使用Specific Primer作为反转录引物。 3. 本制品提供了SYBR Green qPCR分析法和探针qPCR分析法各自适合的反应体系，可以根据分析方法选择体系。 注意：以下是SYBR Green qPCR分析法和探针qPCR分析法进行反转录实验的区别 ① 用于反转录实验RT Primer Mix添加量。 ② 用于反转录实验total RNA的添加量。 4. Real Time RT PCR定量需要建立标准曲线，建立标准曲线的条件就是需要将总RNA和反转录cDNA稀释到较低的浓度。如果用水或TE Buffer稀释时，由于模板浓度低不稳定，因而会缩小曲线范围，结果精度降低。本制品中附加了标准曲线制作用稀释液EASY Dilution (for Real Time PCR)，将Total RNA或cDNA稀释至低浓度时也能够进行准确稀释，容易在宽广范围内获得准确定量的标准曲线。
Invitrogen TRIzol LS Reagent 100ml货号10296-010规格：100mlTRIzol® LS Reagent 特点：• 设计用于液体样品，例如血清和病毒制品• 使用 TRIzol® LS 试剂与液体样品的比值为 3:1• 有助于多种大分子或小分子 RNA 的分离
详细介绍
Invitrogen TRIzol LS Reagent 100ml货号10296-010
TRIzol® LS Reagent 是苯酚和异硫氰酸胍的即用型单相溶液，设计用于从人、动物、植物、酵母、细菌和病毒来源的液体样品中分离总 RNA。 在样品匀浆或裂解过程中，TRIzol® LS Reagent 一方面保持 RNA 的完整性，另一方面破坏细胞并裂解细胞组分。
TRIzol® LS 试剂分离的总 RNA 不含蛋白质和 DNA 杂质。 可用于 Northern blot 分析，点印迹杂交，多聚 (A)+ 选择，体外翻译，RNA 酶保护分析，RT-PCR 和分子克隆。 对于 RT-PCR，当一个外显子中存在两个引物时， 建议用扩增级 DNase I（商品目录号 18068-015）处理分离的 RNA
描述
TRIzol® LS Reagent is a complete, ready-to-use reagent optimized for the isolation of high-quality total RNA, or the simultaneous isolation of RNA, DNA, and protein from a variety of liquid samples. This monophasic solution of phenol and guanidine isothiocyanate is designed to isolate separate fractions of RNA, DNA, and proteins from liquid samples of human, animal, plant, yeast, bacterial, and viral origin, typically within one hour.
• Formulated for use with liquid samples such as serum and virus preparations • Facilitates recovery of RNA, DNA, and protein from a single liquid sample • Offers excellent lysis capability, even with difficult biological fluids
Effectene Transfection Reagent effectene转染试剂对各种类型的真核细胞DNA转染，尤其适用于原代细胞可在血清存在的情况下进行转染适合用于原代细胞或敏感的细胞系高转染效率而且较低的DNA使用量Low endotoxin level with <10 eu>
详细介绍
Effectene Transfection Reagent effectene转染试剂
Principle
Effectene Transfection Reagent is an innovative non-liposomal lipid formulation that is used in conjunction with a special DNA-condensing enhancer and optimized buffer to achieve high transfection efficiencies. The enhancer first condenses the DNA molecules and Effectene Reagent subsequently coats them with cationic lipids （see figure “Model of Effectene Principle”）, providing a particularly efficient way of transferring DNA into eukaryotic cells. Effectene Reagent offers significant advantages over many liposome reagents and other transfection methods （1, 2）.
Procedure
The Effectene procedure has two steps. DNA is first mixed with Enhancer and a buffer that provides optimal salt conditions for efficient DNA condensation. This step requires just 2–5 minutes. Effectene Reagent is then added and the mixture is incubated for 5–10 minutes to allow Effectene–DNA complexes to form. The complexes are mixed with growth medium （which can contain serum and antibiotics）, and added directly to the cells. The cells are then incubated until harvested and analyzed for gene expression （see flowchart “Effectene Transfection Reagent”）.
Applications
Effectene Reagent is suitable for transient and stable transfection of a broad range of cell types （see figures “High Transfection Efficiencies Using Effectene Reagent” and “Transfection of Oligonucleotides Using Effectene Reagent”）. Because transfection can be performed in the presence of serum and requires low amounts of DNA （see figure “Serum and DNA Quantity vs. Transfection Efficiency”）, cytotoxicity is minimal. This makes Effectene Reagent particularly effective for primary cells （see figure “Effectene Reagent with Primary Cells”）. A searchable list of cell lines and primary cells successfully transfected using Effectene Reagent, as well as customer-developed transfection protocols, is available at the Transfection Tools web site — www.qiagen.com/TransfectionTools. View cell lines successfully transfected with this product.
Effectene Transfection Reagent effectene转染试剂
High-throughput transfection
The application of recombinant DNA technology to fields such as drug discovery and development has led to an increased need for high-throughput transfection. Transfection using Effectene Reagent requires low amounts of DNA and minimal handling, and in addition, removal of transfection complexes is not required, making this reagent highly suitable for high-throughput screening. Effectene Reagent provides outstanding transfection efficiencies, excellent reproducibility, and minimal cytotoxicity in high-throughput transfection, and is available in bulk quantities.
Cited References
1. Ausubel, F.M., et al. eds. （1991） Current Protocols in Molecular Biology, Wiley Interscience, New York. 2. Sambrook, J., Fritsch, E.F., and Maniatis, T., eds. （1989） Molecular cloning — a laboratory manual, 2nd Ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor.
Images
Model of Effectene Principle High Transfection Efficiencies Using Effectene Reagent Transfection of Oligonucleotides Using Effectene Reagent Effectene Transfection Procedure Serum and DNA Quantity vs. Transfection Efficiency Effectene Reagent with Primary Cells
提高转染效率的一个简单方法就是提高质粒DNA和转染试剂混合物的用量。然而，由于大多数的转染试剂在转染期间会破坏细胞，因此仅仅提高用量并不是一个明智的选择。而Xfect Transfection Reagent是理想的选择。Xfect是可生物降解的转染聚合物试剂，以毒性低和转染效率高的特性从2,300种侯选物中脱颖而出。Xfect可以提高您的转染效率，同时不会杀死细胞。点击链接观看操作视频Xfect Single Shots Video
Model of Effectene Principle 
High Transfection Efficiencies Using Effectene Reagent 
Transfection of Oligonucleotides Using Effectene Reagent 
Effectene Transfection Procedure 
Serum and DNA Quantity vs. Transfection Efficiency 
Effectene Reagent with Primary Cells
