标签归档：purified

美国BD公司Purified Mouse anti-TAZ抗体Clone M2-616560235

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美国BD公司Purified Mouse anti-TAZ抗体Clone M2-616

  • 产品型号：  560235
  • 简单描述
  • 美国BD公司Purified Mouse anti-TAZ抗体Clone M2-616规格：0.1mg货号：560235库存：现货
详细介绍

美国BD公司Purified Mouse anti-TAZ抗体Clone M2-616

美国BD公司Purified Mouse anti-TAZ抗体Clone M2-616

规格

0.1 mg

货号

560235

目录价

¥ 5,543.00

Brand

BD Pharmingen™

Alternative Name

WWTR1

Concentration

0.5 mg/ml

Isotype

Mouse BALB/c IgG2b, κ

Reactivity

Human (QC Testing)  Mouse (Tested in Development) 

Application

Western blot (Routinely Tested) 
Bioimaging (Tested During Development) 

Immunogen

Human TAZ Recombinant Protein

Storage Buffer

Aqueous buffered solution containing ≤0.09% sodium azide.

详细说明：

 

DESCRIPTION

 

Taz is a 49-kDa transcriptional co-activator with a PDZ-binding motif and is regulated by binding with 14-3-3 proteins. It plays a key role in differentiation of mesenchymal stem cells into either osteoblasts or adipocytes via interactions with key transcription factors Runx2 and PPAR γ . More recently, Taz was found to be a component of an E3 ubiquitin ligase involved in ubiquitin-dependent substrate proteolysis by mediating its interaction with the F-box protein β -Trcp. Therefore, Taz has dual functions of regulating protein degradation and transcription.

 

 

 

 

 

FORMAT

Format

Purified

SUGGESTED COMPANION PRODUCTS

Cat No. Description Size  
611449 HeLa Cell Lysate RUO 500 µg

 
554656 Stain Buffer (FBS) RUO 500 mL

 
554655 Fixation Buffer RUO 100 mL

 
554723 Perm/Wash Buffer RUO 100 mL

 
554002 HRP Goat Anti-Mouse Ig RUO 1 mL

 

RESOURCES & TOOLS

  • Download TDS

PREPARATION AND STORAGE

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

PRODUCT NOTICES

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences。。ｃｏｍ/pharmingen/protocols for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Triton is a trademark of the Dow Chemical Company.
  5. This antibody has been developed and certified for the bioimaging application. However, a routine bioimaging test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.

 

Biolegend LEAF Purified Human CD28单抗302913302914

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Biolegend LEAF Purified Human CD28单抗302913

  • 产品型号：  302914
  • 简单描述
  • Biolegend LEAF Purified Human CD28单抗302913302913 规格：50ug302914 规格：500ug302923 规格：1mg现货！
详细介绍

Biolegend LEAF Purified Human CD28单抗302913

Clone: CD28.2 (See other available formats)
Isotype: Mouse IgG1, κ
Isotype Control: LEAF™ Purified Mouse IgG1, κ Isotype Ctrl
Workshop
Number:		 V-CD28.05
Reactivity: Human, Cross-Reactivity: Chimpanzee, Baboon, Cynomolgus, Rhesus, Pigtailed Macaque, Squirrel Monkey, Capuchin Monkey, Sooty Mangabey
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.1 EU/µg of the protein (<0.01 ng/µg of the protein) as determined by the LAL test.
Preparation: The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: 1.0 mg/ml

Biolegend LEAF Purified Human CD28单抗302913

Storage & Handling: The CD28 antibody solution should be stored undiluted between 2°C and 8°C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: FC – Quality tested
Costim, IP, IHC – Reported in the literature
Recommended Usage: Each lot of this antibody is quality control tested byimmunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Application
Notes:		 Additional reported applications (for the relevant formats) include: immunoprecipitation, immunohistochemical staining of acetone-fixed frozen tissue sections4, and in vitro T cell costimulation5-8. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The CD28.2 antibody co-stimulates T cell proliferation and cytokine production in the presence of suboptimal amounts of anti-CD3 antibody. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 302914). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 302934) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Application
References:		 1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. 
2. Nunes J, et al. 1993. Biochem. J. 293:835. 
3. Calea-Lauri J, et al. 1999. J. Immunol. 163:62.
4. Tazi A, et al. 1999. J. Immunol. 163:3511. (IHC)
5. Marti F, et al. 2001. J. Immunol. 166:197. (Costim)
6. Jeong SH, et al. 2004. J. Virol. 78:6995. (Costim)
7. Rivollier A, et al. 2004. Blood 104:4029. (Costim)
8. Scharschmidt E, et al. 2004. Mol. Cell Biol. 24:3860. (Costim)
9. Sheng W, et al. 2007.Elsevier 580:6819. PubMed
10. Mitsuhashi M. 2007. Clin Chem.53:148. PubMed
11. Ye Z, et al. 2008. Infect. Immun. 76:2541. PubMed
12. Magatti M, et al. 2008. Stem Cells 26:182. (FA) PubMed
  Publication Library
Biolegend LEAF Purified Human CD28单抗302913302914

Human peripheral blood lymphocytes stained with LEAF™ purified CD28.2, followed by anti-mouse IgGs FITC

Biolegend LEAF Purified Human CD28单抗302913302914

Human peripheral blood mononuclear cells were stained with CFSE on day 0, and then stimulated with (filled histogram) or without (open histogram) immobilized LEAF™ Purified CD3 (clone UCHT1) and LEAF™ purified CD28 (clone CD28.2) for 3 days. On day 4, cells were harvested and stained with CD4 Brilliant Violet 711™. Dot plot (above) was analyzed on live cells. Histogram data (below) was analyzed by gating on CD4 positive cells.

Biolegend LEAF Purified Human CD28单抗302913302914

Compare all formats

 
 
 

Antigen Details
 
Description: CD28 is a 44 kD disulfide-linked homodimeric type I glycoprotein. It is a member of the immunoglobulin superfamily and is also known as T44 or Tp44. CD28 is expressed on most T lineage cells, NK cell subsets, and plasma cells. CD28 binds both CD80 and CD86 using a highly conserved motif MYPPY in the CDR3-like loop. CD28 is considered a major co-stimulatory molecule, inducing T lymphocyte activation and IL-2 synthesis, and preventing cell death. In vitro studies indicate that ligation of CD28 on T cells by CD80 and CD86 on antigen presenting cells provides a costimulatory signal required for T cell activation and proliferation.
Other Names: T44, Tp44
Structure: Ig superfamily, type I transmembrane glycoprotein, homodimer, 44 kD
Distribution: Mature T cells, thymocytes, NK cell subsets, plasma cells, EBV-positive B cells
Function: T cell costimulation
Ligand Receptor: CD80, CD86
Antigen
References:		 1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. 
2. June CH, et al. 1994. Immunol. Today 15:321. 
3. Linskey PS, et al. 1993. Annu. Rev. Immunol. 11:191.
GeneID: 940 Biolegend LEAF Purified Human CD28单抗302913302914
UniProt: View information about CD28 on UniProt.org
Keywords: LEAF™ Purified anti-human CD28, CD28.2, LEAF™, T44, Tp44, Human, Cross-Reactivity: Chimpanzee, Baboon, Cynomolgus, Rhesus, Pigtailed Macaque, Squirrel Monkey, Capuchin Monkey, Sooty Mangabey, Activation, Costimulation, Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Biofunctional Assay, Immunology, Antibodies

订购信息：

302913 规格：50ug
302914 规格：500ug
302923 规格：1mg

现货*！

 

Biolegend LEAF™ Purified anti-human CD3 Antibody317315

发表于由

Biolegend LEAF™ Purified anti-human CD3 Antibody

  • 产品型号：  317315
  • 简单描述
  • Clone:OKT3 (See other available formats)Isotype: Mouse IgG2a, κIsotype Control:LEAF™ Purified Mouse IgG2a, κ Isotype Ctrl
详细介绍

LEAF™ Purified anti-human CD3 Antibody

Product Details
 
 
  All CD3 Reagents
Clone: OKT3 （See other available formats）
Isotype: Mouse IgG2a, κ
Isotype Control: LEAF™ Purified Mouse IgG2a, κ Isotype Ctrl
Reactivity: Human
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.1 EU/µg of the protein （<0.01 ng/µg of the protein） as determined by the LAL test.
Preparation: The LEAF™ （Low Endotoxin, Azide-Free） antibody was purified by affinity chromatography.
Concentration: 1.0 mg/ml
Storage & Handling: The CD3 antibody solution should be stored undiluted at 4°C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Application:

FC – Quality tested
IHC, Activ – Reported in the literature
Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
COA:

Enter Lot#:     Biolegend LEAF&trade; Purified anti-human CD3 Antibody317315
Application
Notes:

Clone OKT3 can block the binding of clones SK7 and UCHT1.8 The OKT3 antibody is able to induce T cell activation. Additional reported applications （for the relevant formats） include: immunohistochemical staining of acetone-fixed frozen sections and activation of T cells. The LEAF™ purified antibody （Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered） is recommended for functional assays （Cat. No. 317304）. For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody （Cat. No. 317326） with a lower endotoxin limit than standard LEAF™ purified antibodies （Endotoxin <0.01 EU/µg）.
Application
References:

1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
2. Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York.
3. Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego.
4. Jeong HY, et al. 2008. J. Leuckocyte Biol. 83:755. PubMed
5. Alter G, et al. 2008. J. Virol. 82:9668. PubMed
6. Manevich-Mendelson E, et al. 2009. Blood 114:2344. PubMed
7. Biggs MJ, et al. 2011. J. R. Soc. Interface. 8:1462. PubMed
8. Li B, et al. 2005. Immunology 116:487.
  Publication Library
Biolegend LEAF&trade; Purified anti-human CD3 Antibody317315

Human peripheral blood lymphocytes stained with LEAF™ purified OKT3, followed by anti-mouse IgG FITC

Compare all formats 
 
 
 

Description:

CD3ε is a 20 kD chain of the CD3/T-cell receptor （TCR） complex which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ （CD247）, and a T-cell receptor （α/β or γ/δ） heterodimer. It is found on all mature T lymphocytes, NK-T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.
Other Names: T3, CD3ε
Structure: Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ （CD247） and TCR （α/β or γ/δ） forms CD3/TCR complex, 20 kD
Distribution: Mature T and NK-T cells, thymocyte differentiation
Function: Antigen recognition, signal transduction, T cell activation
Ligand Receptor: Peptide antigen bound to MHC
Antigen
References:

1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego. 
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329. 
3. Lanier L, et al. 1986. J. Immunol. 137:2501.
GeneID: 916 Biolegend LEAF&trade; Purified anti-human CD3 Antibody317315
Latest Publications: View the latest CD3 articles on HighwirePress.com
UniProt: View information about CD3 on UniProt.org
Keywords: LEAF™ Purified anti-human CD3, OKT3, LEAF™, T3, CD3ε
Related Products
 
Description Clone Applications
LEAF™ Purified Mouse IgG2a, κ Isotype Ctrl MOPC-173 FC, WB, IP, ICFC, ICC, IF, IHC, FA
Cell Staining Buffer   FC, ICC, ICFC
RBC Lysis Buffer （10X）   FC, ICFC

Other Formats
 
Description Clone Applications
Alexa Fluor® 488 anti-human CD3 OKT3 FC
Alexa Fluor® 647 anti-human CD3 OKT3 FC
APC anti-human CD3 OKT3 FC
Biotin anti-human CD3 OKT3 FC
Brilliant Violet 510™ anti-human CD3 OKT3 FC
Brilliant Violet 605™ anti-human CD3 OKT3 FC
Brilliant Violet 650™ anti-human CD3 OKT3 FC
Brilliant Violet 711™ anti-human CD3 OKT3 FC
Brilliant Violet 785™ anti-human CD3 OKT3 FC
FITC anti-human CD3 OKT3 FC
Pacific Blue™ anti-human CD3 OKT3 FC
PE anti-human CD3 OKT3 FC
PE/Cy7 anti-human CD3 OKT3 FC
PerCP/Cy5.5 anti-human CD3 OKT3 FC
Purified anti-human CD3 OKT3 FC, IHC
Ultra-LEAF™ Purified anti-human CD3 OKT3 FC, IHC, Activ