标签归档：pharmcia

Pharmcia（GE）葡聚糖凝胶G-15Sephadex G-15Pharmcia17-0020-01

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Pharmcia（GE）葡聚糖凝胶G-15Sephadex G-15

  • 产品型号：  Pharmcia17-0020-01
  • 简单描述
  • Pharmcia（GE)葡聚糖凝胶G-15Sephadex G-15货号：17-0020-02/17-0020-01 品牌：Pharmacia 产品名称：葡聚糖凝胶G-15 17-0020-02/17-0020-01 英文名称：Sephadex G-15 规格：25g/100
详细介绍

Pharmcia（GE)葡聚糖凝胶G-15Sephadex G-15

Sephadex G-15, 100 g

Sephadex G-15 is well established gel filtration medium for desalting and buffer exchange of peptides and small biomolecules >1 500 molecular weight.

  • Quickly desalts, removes contaminants and transfers to a new buffer in a single step.
  • Suitable for buffer exchange and cleanup of biological samples, for example peptides, small proteins, and oligosaccharides larger than 1 500 molecular weight
  • Efficient removal of contaminants such as salts, dyes, and radioactive labels

 

Sephadex is a gel filtration medium prepared by crosslinking dextran with epichlorohydrin. Different types of Sephadex differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex G-15 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex G-50 is available in 4 different particle sizes (Coarse, Medium, Fine & Superfine) and Superfine has the smallest bead size for higher efficiency with shorter diffusion distances. Coarse and Medium are are preferred for large scale group separations at where high flow rates and low operating pressures are required.

 

Pharmcia（GE)葡聚糖凝胶G-15Sephadex G-15

Sephadex G-15, 100 g

Matrix Cross-linked dextran
Particle Size Wet 60 µm-180 µm
Partical Size Dry 40 µm-120 µm
pH Stability Working Range 2-13
Flow Rate < Darcy´s Law1)
Pressure/Flow Specification 20-47 cm/h, pressure drop cm H2O/bed height = 2, bed height 30 cm, 2.6 cm i.d.
Fractionation [Mr] Globular Proteins 1.5 x 103
Fractionation [Mr] Dextrans >< 1.5 x 103
Storage Conditions 4 to 30°C
Autoclavable In wet form (pH 7.0) at 120°C for 30 min
1)The beads behave as rigid spheres obeying Darcy's Law.

Sephadex G-15, 100 g

Handbook
Size Exclusion Chromatography: Principles and Methods Summary

This handbook describes the use of SEC for the purification and separation of biomolecules, with a focus on practical information for obtaining the best results. The media available, selection criteria and examples with detailed instructions for the most common applications are included, as well as the theoretical principles behind the technique. The first step towards a successful separation is to select the correct medium and this handbook focuses on the most up-to-date SEC media and prepacked columns. Close
Magazine
Upstream Downstream issue 2

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Material Safety Data Sheet
Sephadex™ G-15, 100 g

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Sephadex™ G-15, 100 g

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Selection guide
Protein and Peptide Purification: Technique selection guide Summary

This technique selection guide is designed to help you in selecting the technique most suited to your application. It covers ion exchange chromatography (IEX), reversed phase chromatography (RPC), hydrophobic interaction chromatography (HIC), gel filtration (GF), and affinity chromatography (AC). Close

Pharmcia（GE)葡聚糖凝胶G-15Sephadex G-15 详细产品信息可和选购

葡聚糖凝胶G-50Sephadex G-50Pharmcia17-0043-01

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葡聚糖凝胶G-50Sephadex G-50

  • 产品型号：  Pharmcia17-0043-01
  • 简单描述
  • 葡聚糖凝胶G-50Sephadex G-50肽及球形蛋白质：1500～30000；葡聚糖(线性分子)：500～10000
详细介绍

葡聚糖凝胶G-50Sephadex G-50

CAS号：9048-71-9

 

英文名称：Sephadex G-50
其他名称：交联葡聚糖凝胶G-50
CAS号：9048-71-9
干颗粒直径：40～120um
分子量分级范围：A.肽及球形蛋白质：1500～30000；B. 葡聚糖（线性分子）：500～10000
床体积毫升/克干分子筛：9～11ml/g
得水值：5.0±0.3
溶胀zui少平衡时间（h）：室温6h（20℃）；沸水浴2h（90℃）
PH：2～13
性状：白色珠粒，属亲水性凝胶。性稳定，不溶于水、弱酸和碱溶液，遇强酸能使糖苷键分解

葡聚糖凝胶G-50Sephadex G-50

Sephadex G-50 Medium, 100 g

Sephadex G-50 Medium is well established gel filtration medium for desalting and buffer exchange of biomolecules >30 000 molecular weight. The larger bead size of G-50 Medium is suited for large scale separations.

  • Quickly desalts, removes contaminants and transfers to a new buffer in a single step.
  • Suitable for purification of DNA from small molecules by gel filtration
  • Larger bead size for large scale group separations where high flow rates and low operating pressures are required.
  • Classic gel filtration medium.

 

Sephadex is a gel filtration medium prepared by crosslinking dextran with epichlorohydrin. Different types of Sephadex differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex G-50 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex G-50 is available in 4 different particle sizes (Coarse, Medium, Fine & Superfine) and Superfine has the smallest bead size for higher efficiency with shorter diffusion distances. Coarse and Medium are are preferred for large scale group separations where high flow rates and low operating pressures are required.

 

Pharmcia（GE）葡聚糖凝胶G-75Pharmcia17-0050-01

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Pharmcia（GE）葡聚糖凝胶G-75

  • 产品型号：  Pharmcia17-0050-01
  • 简单描述
  • Pharmcia（GE)葡聚糖凝胶G-75葡聚糖凝胶G-75，交联葡聚糖凝胶G-75，交联右旋糖酐凝胶G-75，Sephadex G-75 分离:肽及球形蛋白质：3000～80000；葡聚糖(线性分子)：1000～50000
详细介绍

Pharmcia（GE)葡聚糖凝胶G-75

Sephadex G-75, 100 g

Sephadex G-75 is well established gel filtration medium for desalting and buffer exchange of large biomolecules >80 000 molecular weight.

  • Quickly desalts, removes contaminants and transfers to a new buffer in a single step.
  • Suitable for buffer exchange and cleanup of large biomolecules, >80 000 molecular weight
  • Highest exclusion limit, 80 000, in the Sephadex G-range
  • Classic gel filtration medium.

 

Sephadex is a gel filtration medium prepared by crosslinking dextran with epichlorohydrin. Different types of Sephadex differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex G-10 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex G-50 is available in 4 different particle sizes (Coarse, Medium, Fine & Superfine) and Superfine has the smallest bead size for higher efficiency with shorter diffusion distances. Coarse and Medium are are preferred for large scale group separations where high flow rates and low operating pressures are required.

Pharmcia（GE)葡聚糖凝胶G-75

Sephadex G-75, 100 g

Matrix Cross-linked dextran
Particle Size Wet 90 µm-280 µm
Partical Size Dry 40 µm-120 µm
pH Stability Working Range 2-10
Flow Rate < Darcy´s Law1)
Pressure/Flow Specification min 90 cm/h, bed height 10 cm, column 5 cm i.d.
Fractionation [Mr] Globular Proteins 3 x 103 – 8 x 104
Fractionation [Mr] Dextrans 1 x 103-5 x 104
Exclusion Limit > 7 x 104
Storage Conditions 4 to 30°C
Autoclavable In wet form (pH 7.0) at 120°C for 30 min
1)The beads behave as rigid spheres obeying Darcy's Law.

Pharmcia原装葡聚糖凝胶G-100Sephadex G-100Pharmcia17-0060-01

发表于由

Pharmcia原装葡聚糖凝胶G-100Sephadex G-100

  • 产品型号：  Pharmcia17-0060-01
  • 简单描述
  • Pharmcia原装葡聚糖凝胶G-100Sephadex G-100别名：葡聚糖凝胶G-100，规格：分离：肽及球形蛋白质：4000～150000；葡聚糖(线性分子)：1000～100000包装：10克100克CAS号：9050-94-6保存条件：RT
详细介绍

Pharmcia原装葡聚糖凝胶G-100Sephadex G-100

Matrix Cross-linked dextran
Particle Size Wet 100 µm-310 µm
Partical Size Dry 40 µm-120 µm
Pressure/Flow Specification Care must be taken to avoid compressing the gel. Do not increase the pressure beyond 0.096 bar.
Fractionation [Mr] Globular Proteins 4 x 103-1.5 x 105
Fractionation [Mr] Dextrans 1 x 103-1 x 105
Exclusion Limit > 1.5 x 105
Autoclavable In wet form (pH 7.0) at 120°C for 30 min

Sephadex G-100, 100 g

Application note
Purification and renaturation of recombinant proteins produced in Escherichia coli as inclusion bodies Summary

Expression of eukaryotic genes in the cytoplasm of Escherichia coli often leads to the production and accumulation of the recombinant protein (r-protein) to levels of total cell protein that are higher than when secreted, in some instances by as much as 25%. The r-proteins so produced are frequently in the form of insoluble aggregates, so-called inclusion bodies (IBs). The IBs contain reduced and denatured proteins lacking biological activity. Their sizes and densities are also distinct from most of the bacterial proteins, which facilitates their separation from intracellular E. coli proteins and cell debris by slow speed centrifugation. A general protocol for the extraction and purification of IBs from E. coli cells is shown. The purified IBs are usually dissolved in 6 M guanidine hydrochloride or 8 M urea at near neutral pH prior to re-naturation and purification of the r-protein. The most commonly used procedure for refolding of such de-natured r-proteins is slow dialysis, or dilution into a buffer of near neutral pH. This result in a significant dilution of the r-protein, typically to a few mg of r-protein per mL of solution, with the formation of precipitated proteins being often unavoidable. Alternative purification and/or refolding procedures using gel filtration, ion exchange or hydrophobic interaction chromatography (HIC) have been developed to circumvent such problems. These are outlined briefly in this Application Note. Close
Handbook
Size Exclusion Chromatography: Principles and Methods Summary

This handbook describes the use of SEC for the purification and separation of biomolecules, with a focus on practical information for obtaining the best results. The media available, selection criteria and examples with detailed instructions for the most common applications are included, as well as the theoretical principles behind the technique. The first step towards a successful separation is to select the correct medium and this handbook focuses on the most up-to-date SEC media and prepacked columns. Close
Magazine
Upstream Downstream issue 2

Close
Material Safety Data Sheet
Sephadex™ G-100, 100 g

Close
Sephadex™ G-100, 100 g

Close

Pharmcia原装葡聚糖凝胶G-100Sephadex G-100

17-0060-01 葡聚糖凝胶G-100 Sephadex G-100
17-0050-01 葡聚糖凝胶G-75 Sephadex G-75
17-0043-01 葡聚糖凝胶G-50 Sephadex G-50
17-0033-01 葡聚糖凝胶G-25[中] Sephadex G-25
17-0020-01 葡聚糖凝胶G-15 Sephadex G-15
17-0010-01 葡聚糖凝胶G-10 Sephadex G-10
B-2261 荧光染糖[NO.33342] bisBenzimide H 33342 trihydrochloride
17-0170-01 DEAE葡聚糖凝胶A-25 DEAE Sephadex A-25
17-0170-02 DEAE葡聚糖凝胶A-25 DEAE Sephadex A-25
17-0180-01 DEAE葡聚糖凝胶A-50 DEAE Sephadex A-50
17-0180-02 DEAE葡聚糖凝胶A-50 DEAE Sephadex A-50
G-9382 明胶 Gelatin
L-5125 N-十二烷基肌氨酸钠 N-Lauroylsarcosine sodium salt
L-5125 N-十二烷基肌氨酸钠 N-Lauroylsarcosine sodium salt
0719 N-十二烷基肌氨酸钠 N-Lauroylsarcosine sodium salt
0719 N-十二烷基肌氨酸钠 N-Lauroylsarcosine sodium salt
T-8768 3,3'5,5'-四甲基联苯胺二盐酸 TMB 2Hcl
T-8768 3,3'5,5'-四甲基联苯胺二盐酸 TMB 2Hcl
0759 3,3'5,5'-四甲基联苯胺二盐酸 TMB 2Hcl
0759 3,3'5,5'-四甲基联苯胺二盐酸 TMB 2Hcl
E-2129 埃文斯蓝[伊文斯蓝] Evans Blue
E-2129 埃文斯蓝[伊文斯蓝] Evans Blue
19555 埃文斯蓝[伊文斯蓝] Evans Blue
19555 埃文斯蓝[伊文斯蓝] Evans Blue
0335 蔗糖 Sucrose
21938 蔗糖 Sucrose
F-0127 D-果糖 D-(-)Fructose
0226 D-果糖 D-(-)Fructose
0528 结晶紫 crystal violet
0671 D-核糖 D-Ribose
J392 棉子糖 D-Raffinose
P3504 丽春红S Ponceau S
0860 丽春红S Ponceau S
C-2037 细胞色素C[牛心] Cytochromes C(Bovine Heart)
C-7752 细胞色素C[马心] Cytochromes C(Horse Heart)
0322   EDTA
0322   EDTA
D-4551 N,N-二甲基甲酰胺 N,N-Dimethylformamide
0464 N,N-二甲基甲酰胺 N,N-Dimethylformamide
T-9250 胸腺嘧啶核苷 Thymidine
T-9250 胸腺嘧啶核苷 Thymidine
22305 胸腺嘧啶核苷 Thymidine
22305 胸腺嘧啶核苷 Thymidine
P-3250 木瓜蛋白酶 Papain from papaya latex
P-3250 木瓜蛋白酶 Papain from papaya latex
G-3632 硫酸庆大霉素 Gentamicin sulfate salt
G-3632 硫酸庆大霉素 Gentamicin sulfate salt
0304 硫酸庆大霉素 Gentamicin sulfate salt
0304 硫酸庆大霉素 Gentamicin sulfate salt
C-7698 [环已亚胺] Cycloheximide
C-7698 [环已亚胺] Cycloheximide
D-1756 地塞米松（氢化可的松） Dexamethasone
D-1756 地塞米松（氢化可的松） Dexamethasone
A-7506 维生素C[抗球血酸钠] L-Ascorbic acid[Vitamin C]
A-7506 维生素C[抗球血酸钠] L-Ascorbic acid[Vitamin C]
0764 维生素C[抗球血酸钠] L-Ascorbic acid[Vitamin C]
0764 维生素C[抗球血酸钠] L-Ascorbic acid[Vitamin C]
 ******* 维生素C[抗球血酸钠] L-Ascorbic acid[Vitamin C]
 ******* 氯化锂 Lithium chloride
0416 氯化锂 Lithium chloride
22178 纤维素酶 Cellulase from Aspergillus niger
**** 纤维素酶 Cellulase from Aspergillus niger
M-8410 矿物油[石腊油] Mineral oil
J217 矿物油[石腊油] Mineral oil
C-7880 L-半胱氨酸盐酸盐 L-Cysteine hydrochloride monohydrate
C-7880 L-半胱氨酸盐酸盐 L-Cysteine hydrochloride monohydrate
0206 L-半胱氨酸盐酸盐 L-Cysteine hydrochloride monohydrate
0206 L-半胱氨酸盐酸盐 L-Cysteine hydrochloride monohydrate
E-4378 乙二醇-双-(2-氨基乙基)四乙酸 Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid[EGTA]
0732 乙二醇-双-(2-氨基乙基)四乙酸 Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid[EGTA]
T-8877 2,3,5-氯化三苯基四氮唑 2,3,5-Triphenyltetrazolium chloride[TTC]
0765 2,3,5-氯化三苯基四氮唑 2,3,5-Triphenyltetrazolium chloride[TTC]
B-0252 硼酸 Boric acid
0588 硼酸 Boric acid
P-3640 聚乙二醇 3350 PEG 3350
P-3640 聚乙二醇 3350 PEG 3350
817006 聚乙二醇 4000 PEG 4000
817006 聚乙二醇 4000 PEG 4000
807491 聚乙二醇 6000 PEG 6000
807491 聚乙二醇 6000 PEG 6000
0159 聚乙二醇 8000 PEG 8000
0159 聚乙二醇 8000 PEG 8000
81285 聚乙二醇 12000 PEG 12000
81285 聚乙二醇 12000 PEG 12000
818897 聚乙二醇 20000 PEG 20000
818897 聚乙二醇 20000 PEG 20000
K-3378 激动素[6-呋喃氨基嘌呤] 6-Furfuryl Aminopurine(Kinetin)[KT]
K-3378 激动素[6-呋喃氨基嘌呤] 6-Furfuryl Aminopurine(Kinetin)[KT]
17980 激动素[6-呋喃氨基嘌呤] 6-Furfuryl Aminopurine(Kinetin)[KT]
17980 激动素[6-呋喃氨基嘌呤] 6-Furfuryl Aminopurine(Kinetin)[KT]
N-3503 制霉菌素 Nystatin
N-3503 制霉菌素 Nystatin
19770 制霉菌素 Nystatin
19770 制霉菌素 Nystatin
D-5637 3,3'-二氨基联苯胺 3,3′-Diaminobenzidine tetrahydrochloride hydrate[DAB]
D-5637 3,3'-二氨基联苯胺 3,3′-Diaminobenzidine tetrahydrochloride hydrate[DAB]
0430 3,3'-二氨基联苯胺 3,3′-Diaminobenzidine tetrahydrochloride hydrate[DAB]
0430 3,3'-二氨基联苯胺 3,3′-Diaminobenzidine tetrahydrochloride hydrate[DAB]
I-2886 吲哚-3-乙酸 3-Indoleacetic acid[IAA]
I-2886 吲哚-3-乙酸 3-Indoleacetic acid[IAA]
17550 吲哚-3-乙酸 3-Indoleacetic acid[IAA]
17550 吲哚-3-乙酸 3-Indoleacetic acid[IAA]
A-5006 L-精氨酸 L-Arginine
A-5006 L-精氨酸 L-Arginine
11490 L-精氨酸 L-Arginine
11490 L-精氨酸 L-Arginine
M-9625 L-甲硫氨酸[L-蛋氨酸] L-Methionine
M-9625 L-甲硫氨酸[L-蛋氨酸] L-Methionine
18915 L-甲硫氨酸[L-蛋氨酸] L-Methionine
18915 L-甲硫氨酸[L-蛋氨酸] L-Methionine
G-8270 D-葡萄糖[无水] D-(+)-Glucose
0188 D-葡萄糖[无水] D-(+)-Glucose
F-9037 去离子甲酰胺 Formamide,Deionized
0606 去离子甲酰胺 Formamide,Deionized