标签归档：mouse

日本同仁化学DirectPCR Lysis Reagent （Mouse Tail）鼠尾直接PCR裂解液102-T(现货)- DOJINDO

发表于由

上海金畔生物科技有限公司代理日本同仁化学 DOJINDO代理商全线产品，欢迎访问官网了解更多信息

DirectPCR Lysis Reagent （Mouse Tail）鼠尾直接PCR裂解液
【单瓶试剂，组织裂解+PCR一步到位】

产品关键词：
Viagen Biotech; DirectPCR® 直接PCR；PCR genotyping 基因分型；Proteinase K蛋白酶K；102-T (101-T)；
产品介绍：
Viagen DirectPCR® DNA Extraction System（DirectPCR® DNA提取系统）是一种单管系统，用于从小鼠尾巴，耳片，卵黄囊和培养细胞内快递制备DNA。Viagen Biotech, Inc科学家开发的组分（正在申请）允许用DirectPCR® DNA Extraction System获得的DNA提取物与基因分型用的基因组PCR兼容。生物样本的粗提物与许多分子生物学级的试剂比如PCR都难以兼容，部分原因在于粗提物内含有抑制剂。DirectPCR®不仅介导生物样品的快速裂解，而且含有抑制剂，能够有效抑制PCR扩增用粗提裂解物中存在的抑制效应，同时zui大程度保持释放出来的基因组DNA的完整性。申请中的DirectPCR®操作起来，完全消除任何溶液转移或开管步骤，根本上节省了大量时间和精力。
产品特点：
1）省时：更少的动手时间；尾巴裂解液粗提物即可用于PCR；
2）省钱：仅需成本的反应流程；
3）安全：无有机试剂；
4）环保：更少消耗（有机试剂，管子，枪头等等）
5）可靠有效：100%成功率保证高产量
应用实例

简单流程：
1）用DirectPCR®裂解鼠尾；
2）85℃孵育45min；
3）取1μl裂解液进行PCR基因型研究
订购信息：

品牌 产品名称 货号 规格
Viagen Biotech DirectPCR Lysis Reagent (Mouse Tail) 101-T 50ml（up to 250 tails）
Viagen Biotech DirectPCR Lysis Reagent (Mouse Tail) 102-T(现货) 100ml（up to 500 tails）
Viagen Biotech Genomic PCR Grade Proteinase K Solution 501-PK 5ml

biolegend 444107 mouse MIF elisa kit代理

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biolegend 444107 mouse MIF elisa kit代理

  • 产品型号：  
  • 简单描述
  • biolegend 444107 mouse MIF elisa kit代理规格：96T 预包被现货供应
详细介绍

biolegend 444107 mouse MIF elisa kit代理

Kit Contents
 
  • Anti-Mouse MIF Pre-coated 96 well Strip Microplate
  • Mouse MIF Detection Antibody
  • Mouse MIF Standard
  • Avidin-HRP
  • Assay Buffer B
  • Wash Buffer (20X)
  • Substrate Solution F
  • Stop Solution
  • Plate Sealers

biolegend 444107 mouse MIF elisa kit代理

Product Details
 
Application: ELISA
Standard Range: 156 – 10,000 pg/mL
Sensitivity: 25.28 ± 1.68 pg/mL

Description:

Macrophage migration inhibitory factor (MIF), also known as glycosylation inhibiting factor (GIF), is a pituitary hormone and a macrophage- and T cell-derived cytokine that plays an important role in the endocrine and immune systems. MIF consists of 115 amino acids and has a molecular weight of 12.5 kD. Mouse MIF shares most of its identity with human (90%), rat (99%), and cattle (88%) MIFs, and a moderate percentage of its identity with MIFs from a wide variety of other species. It exists in monomeric, dimeric, and trimeric forms of identical protomers.

MIF is active as a pro-inflammatory regulator and upregulates multiple cytokines that are vital to combating infections in vivo, including tumor-necrosis factor (TNF), interferon-γ (IFN-γ), and interleukin-12 (IL-12). It is known to maintain cell survival and promote pro-inflammatory immune responses. MIF also activates the ERK1/ERK2 signaling pathway, which inhibits the immunosuppressive effects of glucocorticoids. MIF also plays a critical role in superantigen-induced toxic shock. It upregulates several factors that enhance the growth and invasiveness of gram-negative bacteria. MIF can enhance many inflammatory diseases, such as acute respiratory distress syndrome, arthritis, atopic dermatitis, allograft rejection, glomerulonephritis, inflammatory bowel diseases, asthma, and atherosclerosis. Known receptors for MIF include CXCR2, CXCR4, CD44, and CD74.

The LEGEND MAX™ Mouse MIF ELISA kit is a ready-to-use Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with a 96-well strip plate that is pre-coated with a rat monoclonal anti-mouse MIF antibody. The Detection Antibody is a biotinylated goat polyclonal anti-mouse MIF antibody. This assay is specifically designed to quantify mouse MIF from serum, plasma, cell culture supernatant, and cell lysate. This kit is analytically validated with prepared reagents.

 

Miltenyi美天尼小鼠CD45R微珠mouse CD45R （B220） MicroBeads130-049-501

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Miltenyi美天尼小鼠CD45R微珠mouse CD45R （B220） MicroBeads

  • 产品型号：  130-049-501
  • 简单描述
  • Miltenyi美天尼小鼠CD45R微珠mouse CD45R （B220） MicroBeads品牌：Miltenyi Biotec货号：130-049-501抗体名：CD45R （B220） MicroBeads, mouse抗体英文名：CD45R （B220） MicroBeads, mouse靶点：详询浓度：详询应用范围：详询宿主：详询适应物种：详询
详细介绍

Miltenyi美天尼小鼠CD45R微珠mouse CD45R (B220) MicroBeads

Mouse CD45R (B220) MicroBeads were developed for the positive selection or depletion of mouse B cells from various lymphoid tissues like bone marrow, lymph nodes, and spleen or from non-lymphoid sources like lamina propria, peritoneal cavity, or lung.

Miltenyi美天尼小鼠CD45R微珠mouse CD45R (B220) MicroBeads

详细信息可致电本公司

Miltenyi美天尼小鼠CD45R微珠mouse CD45R (B220) MicroBeads

订购信息：

CD45R (B220) MicroBeads, mouse

Capacity: for 2×109 total cells

Data sheet130-049-501price on request here

CD45R (B220) MicroBeads, mouse – lyophilized

Capacity: for 2×109 total cells

Data sheet130-097-152price on request here

美国BD公司Purified Mouse anti-TAZ抗体Clone M2-616560235

发表于由

美国BD公司Purified Mouse anti-TAZ抗体Clone M2-616

  • 产品型号：  560235
  • 简单描述
  • 美国BD公司Purified Mouse anti-TAZ抗体Clone M2-616规格：0.1mg货号：560235库存：现货
详细介绍

美国BD公司Purified Mouse anti-TAZ抗体Clone M2-616

美国BD公司Purified Mouse anti-TAZ抗体Clone M2-616

规格

0.1 mg

货号

560235

目录价

¥ 5,543.00

Brand

BD Pharmingen™

Alternative Name

WWTR1

Concentration

0.5 mg/ml

Isotype

Mouse BALB/c IgG2b, κ

Reactivity

Human (QC Testing)  Mouse (Tested in Development) 

Application

Western blot (Routinely Tested) 
Bioimaging (Tested During Development) 

Immunogen

Human TAZ Recombinant Protein

Storage Buffer

Aqueous buffered solution containing ≤0.09% sodium azide.

详细说明：

 

DESCRIPTION

 

Taz is a 49-kDa transcriptional co-activator with a PDZ-binding motif and is regulated by binding with 14-3-3 proteins. It plays a key role in differentiation of mesenchymal stem cells into either osteoblasts or adipocytes via interactions with key transcription factors Runx2 and PPAR γ . More recently, Taz was found to be a component of an E3 ubiquitin ligase involved in ubiquitin-dependent substrate proteolysis by mediating its interaction with the F-box protein β -Trcp. Therefore, Taz has dual functions of regulating protein degradation and transcription.

 

 

 

 

 

FORMAT

Format

Purified

SUGGESTED COMPANION PRODUCTS

Cat No. Description Size  
611449 HeLa Cell Lysate RUO 500 µg

 
554656 Stain Buffer (FBS) RUO 500 mL

 
554655 Fixation Buffer RUO 100 mL

 
554723 Perm/Wash Buffer RUO 100 mL

 
554002 HRP Goat Anti-Mouse Ig RUO 1 mL

 

RESOURCES & TOOLS

  • Download TDS

PREPARATION AND STORAGE

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

PRODUCT NOTICES

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences。。ｃｏｍ/pharmingen/protocols for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Triton is a trademark of the Dow Chemical Company.
  5. This antibody has been developed and certified for the bioimaging application. However, a routine bioimaging test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.

 

BD代理APC Mouse IgG2b κ Isotype Control Clone 27-555745

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BD代理APC Mouse IgG2b κ Isotype Control Clone 27-

  • 产品型号：  555745
  • 简单描述
  • BD代理APC Mouse IgG2b κ Isotype Control Clone 27-35规格：100T现货供应中文名：APC标记Mouse IgG2b κ抗体
详细介绍

BD代理APC Mouse IgG2b κ Isotype Control   Clone  27-35货号：555745
规格：100T
现货供应
中文名：APC标记Mouse IgG2b κ抗体

BD代理APC Mouse IgG2b κ Isotype Control   Clone  27-35货号：555745

Brand

BD Pharmingen™

Alternative Name

Anti-Dansyl

Vol. Per Test

20 µl

Isotype

Mouse C.SW IgG2b, κ

Application

Isotype control, Flow cytometry (Routinely Tested) 

Storage Buffer

Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Regulatory Status

RUO

BD代理APC Mouse IgG2b κ Isotype Control   Clone  27-35货号：555745

产品详情：

INTENDED USE

 

The mouse IgG2b, κ immunoglobulin isotype control monoclonal antibody 27-35 is specific for the hapten dansyl (5-[dimethylamino] naphthalene-1-sulfonyl). This hapten is not expressed on human cells or human cell lines. The 27-35 immunoglobulin was selected as an isotype control following testing which demonstrated low background staining on a variety of mouse and human tissues.

 

 

FORMAT

BD代理APC Mouse IgG2b κ Isotype Control Clone 27-555745

Format

APC

Excitation Source

Red 633 nm

Excitation Max

650 nm

Emission Max

660 nm

Allophycocyanin (APC), is an accessory photosynthetic pigment found in blue-green algae. Its molecular weight is approximay 105 kDa. APC has six phycocyanobilin chromophores per molecule, which makes it a very bright fluorochrome that is highly suitable for flow cytometry applications. Due to nearly identical excitation and emission properties but different spillover characteristics, APC and Alexa Fluor® 647 cannot be used simultaneously

Other Formats →

  • APC-Cy™7
  • APC-H7
  • APC-R700
  • Alexa Fluor® 488
  • Alexa Fluor® 647
  • Alexa Fluor® 700
  • BB515
  • BUV395
  • BUV563
  • BUV737
  • BV421
  • BV510
  • BV650
  • BV711
  • BV786
  • Biotin
  • FITC
  • PE
  • PE-CF594
  • PE-Cy™5

RESOURCES & TOOLS

  • Download TDS

STORAGE AND HANDLING

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

PRODUCT NOTICES

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  3. Please refer to www.bdbiosciences。。ｃｏｍ/pharmingen/protocols for technical protocols.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences。。ｃｏｍ/colors.
  5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  6. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

BD PerCP-Cy5.5 Mouse Anti-Human CD56抗体Clone B159560842

发表于由

BD PerCP-Cy5.5 Mouse Anti-Human CD56抗体Clone B159

  • 产品型号：  560842
  • 简单描述
  • BD PerCP-Cy5.5 Mouse Anti-Human CD56抗体Clone B159宿主物种：mouse反应物中：human规格：100T克隆号：B159应用：流逝现货供应中
详细介绍

BD PerCP-Cy5.5 Mouse Anti-Human CD56抗体Clone  B159

Brand：BD Pharmingen

Alternative Name：NCAM1; NCAM-1; Neural cell adhesion molecule 1; NCAM; NKH1; MSK39

Application：Flow cytometry (Routinely Tested) 

Immunogen：Human NK Cells

Storage Buffer：Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

 

BD PerCP-Cy5.5 Mouse Anti-Human CD56抗体Clone  B159

 

DESCRIPTION

 

The B159 monoclonal antibody specifically binds to CD56. CD56 is a heavily glycosylated adhesion protein that is present on a subpopulation of peripheral blood large granular lymphocytes that demonstrate natural killer activity. CD56 is also expressed on a subset of T cells but is not expressed on myeloid cells, erythrocytes or B cells. This antigen is a pan-NK-cell marker. CD56 is virtually identical to an isoform of the neural cell adhesion molecule (NCAM), a structure mediating homotypic and heterotypic cell-cell interactions.

 

 

 

 

 

 

FORMAT

BD PerCP-Cy5.5 Mouse Anti-Human CD56抗体Clone B159560842

Format

PerCP-Cy™5.5

Excitation Source

Blue 488 nm

Excitation Max

482 nm

Emission Max

695 nm

PerCP-Cy™5.5 is a tandem conjugate that combines PerCP with a cyanine dye. PerCP-Cy5.5 is not subject to photobeaching like PerCP and can be used with stream-in-air flow cytometers. It has less Fc receptor–mediated nonspecific staining than PE-Cy5. Additionally, the PerCP-Cy5.5 tandem conjugate is not as susceptible to fixative or light instability compared to APC-Cy7 and PE-Cy7.

Other Formats →

  • APC
  • Alexa Fluor® 488
  • Alexa Fluor® 647
  • Alexa Fluor® 700
  • BB515
  • Biotin
  • FITC
  • NA/LE
  • PE
  • PE-CF594
  • PE-Cy™5
  • PE-Cy™7
  • Purified
  • V450

    SUGGESTED COMPANION PRODUCTS

    Cat No. Description Size  
    550795 PerCP-Cy™5.5 Mouse IgG1 κ Isotype Control RUO 0.1 mg  
    555899 Lysing Buffer RUO 100 mL  

    RESOURCES & TOOLS

    • Spectrum Viewer
    • Panel Designer
    • Download TDS

    PREPARATION AND STORAGE

    Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with PerCP-Cy5.5 under optimum conditions, and unconjugated antibody and free PerCP-Cy5.5 were removed. Storage of PerCP-Cy5.5 conjugates in unoptimized diluent is not recommended and may result in loss of signal intensity.

     

    DAKO CD20抗体（Mouse Anti-Human CD20cy）M0755

    发表于由

    DAKO CD20抗体（Mouse Anti-Human CD20cy）

    • 产品型号：  M0755
    • 简单描述
    • DAKO CD20抗体（Mouse Anti-Human CD20cy）规格：0.2ml单克隆小鼠抗人CD20cy抗体*!
    详细介绍

    DAKO CD20抗体(Mouse Anti-Human CD20cy）

    克隆号：L26

    该抗体可与一种非常有用的正常和肿瘤性B细胞的非常有用标记的抗原反应。该抗体能与位于CD20抗原上的一种胞质内表位反应，该抗体可与DAKO抗CD3多克隆抗体相配合用于鉴别T细胞和B细胞。该抗体有有助于鉴别霍奇金淋巴瘤中的霍奇金细胞和Read-sternberg细胞

    DAKO CD20抗体(Mouse Anti-Human CD20cy）

     

    现货供应*！

    DAKO（丹科）CD4抗体（Mouse anti-human CD4）M7310

    发表于由

    DAKO（丹科）CD4抗体（Mouse anti-human CD4）

    • 产品型号：  M7310
    • 简单描述
    • DAKO（丹科）CD4抗体（Mouse anti-human CD4）规格：0.2ml克隆号：Clone 4B12英文名：Monoclonal Mouse Anti-Human CD4 Clone 4B12现货*！
    详细介绍

    DAKO（丹科）CD4抗体（Mouse anti-human CD4）

    英文名：Monoclonal Mouse Anti-Human CD4 Clone 4B12

    克隆号：Clone 4B12

    特别适用于：冰冻切片和石蜡切片

    DAKO（丹科）CD4抗体（Mouse anti-human CD4）

    CD4是一种在正常胸腺细胞、T辅助细胞、大多数成熟外周T细胞以及抑制性或细胞毒性T细胞亚类中表达的跨膜糖蛋白。未在未成熟的胸腺细胞中发现CD4。该抗体有助于鉴别间变性大细胞淋巴瘤、非特定外周T细胞淋巴瘤和肉芽肿病

    Peripheral T-cell lymphoma. The majority of the neoplastic cells show a strong distinct membranous staining reaction.

    Clinical application for IR649/IS649. For identification of thymocytes, T-helper cells and classification of related tumors such as peripheral T-cell lymphoma (unspecified), anaplastic large cell lymphoma and mycosis fungoides.

     

    DAKO Monoclonal Mouse Anti-Human CD45R0抗体M0742

    发表于由

    DAKO Monoclonal Mouse Anti-Human CD45R0抗体

    • 产品型号：  M0742
    • 简单描述
    • DAKO Monoclonal Mouse Anti-Human CD45R0抗体Clone UCHL1规格：1ml
    详细介绍

    DAKO Monoclonal Mouse Anti-Human CD45R0抗体

    CD45 is a transmembrane glycoprotein expressed on most nucleated cells of hematopoetic origin. On human leucocytes, five different isoforms of CD45 have been identified, named ABC, AB, BC, B and 0. This antibody reacts with an epitope unique for CD45R0. The antibody labels most thymocytes, a subpopulation of resting T cells within both CD4 and CD8 subsets, and mature, activated T cells. It is effective on formalin-fixed, paraffin-embedded tissue sections. Results aid in the classification of T-cell neoplasms.

    DAKO Monoclonal Mouse Anti-Human CD45R0抗体

    我公司代理DAKO产品，详细产品信息可和选购！

    DAKO抗体是丹麦的著名抗体生产商，提供世界上质量zui稳定，zui为经典的抗体。其主要产品涉及免疫化学、流式细胞，免疫组织化学，原位杂交，微生物检验等。DAKO的不同显色系统，Envision，LSAB和免疫细胞化学的CAS，GenpointTM和用于分子病理学的PNA=ISH试剂盒，和用于ELISA的AMPAKTM/AmpliQ是其在生物化学研究中的革新产品 。

     

     

    BD四型胶原蛋白COLLAGEN IV MOUSE NATURAL354233

    发表于由

    BD四型胶原蛋白COLLAGEN IV MOUSE NATURAL

    • 产品型号：  354233
    • 简单描述
    • BD四型胶原蛋白COLLAGEN IV MOUSE NATURAL 规格：1mg/瓶主要用途：培养板包被等细胞培养用级别：ECM
    详细介绍

    BD四型胶原蛋白COLLAGEN IV MOUSE NATURAL

    主要用途：培养板包被等细胞培养用

    现货*

    BD四型胶原蛋白COLLAGEN IV MOUSE NATURAL

    详细描述

    BD四型胶原蛋白COLLAGEN IV MOUSE NATURAL354233

    Corning? Collagen IV, mouse, 1mg, is used as a thin coating on tissue-culture surfaces to promote cell attachment and proliferation and to study the collagen’s effects on cell behavior.  

    Source
    Source material tested for HBsAG, for anti-HIV-1 and anti-HIV-2, and anti-HCV virus-1 and virus-2.

    Quality
    Electrophoretically homogeneous (SDS-PAGE)
    Tested and found negative for bacteria, fungi, and mycoplasma

    Preparation and Storage
    Stable for at least three months at -70°C. Avoid multiple freeze-thaws.

    Handling
    Recommended concentration: 1-5 μg/cm2 of growth surface depending on cell type

    Formulation
    Frozen in 0.05 M HCI

    product details at-a-glance

    Qty./Pk.: 1
    Qty./CS.: 1
    Size: 1 mg
    Shipping: Refrigerated Shipping DI

    R&D Recombinant Human/mouse IL-17A重组人/小鼠白介17细胞因子RD 317-ILB-050

    发表于由

    R&D Recombinant Human/mouse IL-17A重组人/小鼠白介17细胞因子

    • 产品型号：  RD 317-ILB-050
    • 简单描述
    • 产品详细信息请
    详细介绍

    Activity Measured by its ability to induce IL6
    secretion by NHDF human normal dermal fibroblasts.
    The ED50 for this effect is typically 26
    ng/mL.
    Measured by its ability to induce IL6
    secretion by NIH-3T3 mouse embryonic fibroblast cells. Yao, Z. et al. （1995） Immunity 3:811.
    The ED50 for this effect is typically 1.57.5
    ng/mL.
    Endotoxin Level <1.0 EU per 1 μg of the protein by the LAL method.
    Purity >97%, by SDSPAGE
    under reducing conditions and visualized by silver stain.
    Formulation Lyophilized from a 0.2 μm filtered solution in HCl. See Certificate of Analysis for details.
    PREPARATION AND STORAGE
    Reconstitution Reconstitute at 100 μg/mL in 4 mM HCI.
    Shipping The product is shipped with polar packs. Upon receipt, store it immediay at the temperature recommended below.
    Stability & Storage Use a manual defrost freezer and avoid repeated freezethaw
    cycles.
    l 12 months from date of receipt, 20
    to 70
    °C as supplied.
    l 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    l 3 months, 20
    to 70
    °C under sterile conditions after reconstitution.
    BACKGROUND
    Interleukin 17 （also known as CTLA8）
    is a T cellexpressed
    pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of
    herpesvirus Saimiri. cDNA clones encoding IL17
    have been isolated from activated rat, mouse and human T cells. Human IL17
    cDNA encodes a 155 amino acid （aa）
    residue precursor protein with a 19 amino acid residue signal peptide that is cleaved to yield the 136 aa residue mature IL17
    containing one potential Nlinked
    glycosylation site. Both recombinant and natural IL17
    have been shown to exist as disulfidelinked
    homodimers. At the amino acid level, human IL17
    shows 72% and
    63% sequence identity with herpesvirus and rat IL17,
    respectively. An IL17
    specific mouse cell surface receptor （IL17
    R） has been cloned. While the expression of
    IL17
    mRNA is restricted to activated T cells, the expression of mIL17
    R mRNA has been detected in virtually all cells and tissues tested. IL17
    exhibits multiple
    biological activities on a variety of cells including the induction of IL6
    and IL8
    production in fibroblasts, the enhancement of surface expression of ICAM1
    in
    fibroblasts, activation of NFκB
    and costimulation of T cell proliferation. The nonglycosylated,
    E. coliexpressed
    rhIL17
    has been shown to be active on human as
    well as mouse cell lines.
    References:
    1. Yao, Z. et al. （1995） J. Immunol. 155:5483.
    2. Yao, Z. et al. （1995） Immunity 3:811.
    3. Rouvier, E. et al. （1993） J. Immunol. 150:5445

     

    M-CSF的重组小鼠细胞因子Recombinant Mouse M-CSFRD 416-ml-010

    发表于由

    M-CSF的重组小鼠细胞因子Recombinant Mouse M-CSF

    • 产品型号：  RD 416-ml-010
    • 简单描述
    • M-CSF的重组小鼠细胞因子Recombinant Mouse M-CSF也被称为CSF1细胞因子，巨噬细胞的存活，增殖和分化（1）主要监管机构纤细说明书在产品详细介绍中
    详细介绍

    M-CSF的重组小鼠细胞因子Recombinant Mouse M-CSF

    DESCRIPTION
    Source E. coliderived
    Lys33Glu262,
    with an Nterminal
    Met
    Accession # Q3U4F9
    Nterminal
    Sequence
    Analysis
    Met
    Structure / Form Disulfidelinked
    homodimer
    Predicted Molecular
    Mass
    26 kDa （monomer）
    SPECIFICATIONS
    SDSPAGE
    29 kDa, reducing conditions
    Activity Measured in a cell proliferation assay using M-NFS-60 mouse myelogenous leukemia lymphoblast cells. Halenbeck, R. et al. （1989）
    Biotechnology 7:710.
    The ED50 for this effect is typically 0.5-3 ng/mL.
    Endotoxin Level <1.0 EU per 1 μg of the protein by the LAL method.
    Purity >97%, by SDSPAGE
    under reducing conditions and visualized by silver stain.
    Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. See Certificate of Analysis for details.
    PREPARATION AND STORAGE
    Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
    Shipping The product is shipped at ambient temperature. Upon receipt, store it immediay at the temperature recommended below.
    Stability & Storage Use a manual defrost freezer and avoid repeated freezethaw
    cycles.
    l 12 months from date of receipt, 20
    to 70
    °C as supplied.
    l 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    l 3 months, 20
    to 70
    °C under sterile conditions after reconstitution.
    BACKGROUND
    MCSF,
    also known as CSF1,
    is a fourαhelicalbundle
    cytokine that is the primary regulator of macrophage survival, proliferation and differentiation （1 3）.
    MCSF
    is also essential for the survival and proliferation of osteoclast progenitors （1, 4）. MCSF
    also primes and enhances macrophage killing of tumor cells and
    microorganisms, regulates the release of cytokines and other inflammatory modulators from macrophages, and stimulates pinocytosis （2, 3）. MCSF
    increases during
    pregnancy to support implantation and growth of the decidua and placenta （5）. Sources of MCSF
    include fibroblasts, activated macrophages, endometrial secretory
    epithelium, bone marrow stromal cells and activated endothelial cells （1 5）.
    The MCSF
    receptor （cfms）
    transduces its pleotropic effects and mediates its
    endocytosis. MCSF
    mRNAs of various sizes occur （3 9）.
    Full length mouse MCSF
    transcripts encode a 520 amino acid （aa） type I transmembrane （TM） protein with
    a 462 aa extracellular region, a 21 aa TM domain, and a 37 aa cytoplasmic tail that forms a 140 kDa covalent dimer. Differential processing produces two
    proteolytically cleaved, secreted dimers. One is an Nand
    Oglycosylated
    86 kDa dimer, while the other is modified by both glycosylation and chondroitinsulfate
    proteoglycan （PG） to generate a 200 kDa subunit. Although PGmodified
    MCSF
    can circulate, it may be immobilized by attachment to type V collagen （8）. Shorter
    transcripts encode M CSF
    that lacks cleavage and PG sites and produces an Nglycosylated
    68 kDa TM dimer and a slowly produced 44 kDa secreted dimer （7）.
    Although forms may vary in activity and halflife,
    all contain the Nterminal
    150 aa portion that is necessary and sufficient for interaction with the M CSF
    receptor
    （10, 11）. The first 229 aa of mature mouse MCSF
    shares 87%, 83%, 82% and 81% aa identity with corresponding regions of rat, dog, cow and human MCSF,
    respectively （12, 13）. Human MCSF
    is active in the mouse, but mouse MCSF
    is reported to be speciesspecific.
    M-CSF的重组小鼠细胞因子Recombinant Mouse M-CSF

    References:
    1. Pixley, F.J. and E.R. Stanley （2004） Trends Cell Biol. 14:628.
    2. Chitu, V. and E.R. Stanley （2006） Curr. Opin. Immunol. 18:39.
    3. Fixe, P. and V. Praloran （1997） Eur. Cytokine Netw. 8:125.
    4. Ryan, G.R. et al. （2001） Blood 98:74.
    5. Makrigiannakis, A. et al. （2006） Trends Endocrinol. Metab. 17:178.
    6. Nandi, S. et al. （2006） Blood 107:786.
    7. Rettenmier, C.W. and M.F. Roussel （1988） Mol. Cell Biol. 8:5026.
    8. Suzu, S. et al. （1992） J. Biol. Chem. 267:16812.
    9. Manos, M.M. （1988） Mol. Cell. Biol. 8:5035.
    10. Koths, K. （1997） Mol. Reprod. Dev. 46:31.
    11. Jang, MH.
    et al. （2006） J. Immunol. 177:4055.
    12. DeLamarter, J.F. et al. （1987） Nucleic Acids Res. 15:2389.
    13. Ladner, M.B. et al. （1988） Proc. Natl. Acad. Sci. USA 85:6706.

     

    Anti-Mouse F4/80 Antigen PE 抗小鼠F4/80抗原PE抗体eBiosience12-4801-82

    发表于由

    Anti-Mouse F4/80 Antigen PE 抗小鼠F4/80抗原PE抗体

    • 产品型号：  eBiosience12-4801-82
    • 简单描述
    • HostRatIsotypeIgG2a, kappaReactivityMouseConjugatePELaserBlue Laser, Green Laser, Yellow-Green LaserEmit575 nmExcite488 – 561 nmReported ApplicationsFlow Cytometric Analysis
    详细介绍

    Also known as: Pan Macrophage Marker

    Clone: BM8

    Description: The BM8 monoclonal antibody reacts with mouse F4/80 antigen, an approximay 125 kDa transmembrane protein. The F4/80 antigen is expressed by a majority of mature macrophages and is the best marker for this population of cells. However, other cell types such as Langerhans cells and liver Kupffer cells have been reported to express this antigen. Expression of F4/80 commences during early myeloid development and is upregulated on all BM cells stimulated in vitro with M-CSF. It has been shown that some cytokines downregulate the expression of F4/80 resulting in lack of F4/80 antigen on a subpopulation of macrophages, especially in the lymphoid microenvironment in vivo

    Host Rat
    Isotype IgG2a, kappa
    Reactivity Mouse
    Conjugate PE
    Laser Blue Laser, Green Laser, Yellow-Green Laser
    Emit 575 nm
    Excite 488 – 561 nm
    Reported Applications Flow Cytometric Analysis

    Recombinant Mouse M-CSF巨噬细胞集落刺激因子R&D 416-ml

    发表于由

    Recombinant Mouse M-CSF巨噬细胞集落刺激因子

    • 产品型号：  R&D 416-ml
    • 简单描述
    • Recombinant Mouse M-CSF巨噬细胞集落刺激因子上海金畔代理R&D产品，美国*Recombinant Mouse M-CSF，Recombinant human M-CSF，规格分别是10ug和50ug 咨询还有人的Human M-CSF
    详细介绍

    Recombinant Mouse M-CSF巨噬细胞集落刺激因子

    MCSF,
    also known as CSF1,
    is a fourαhelicalbundle
    cytokine that is the primary regulator of macrophage survival, proliferation and differentiation （1 3）.
    MCSF
    is also essential for the survival and proliferation of osteoclast progenitors （1, 4）. MCSF
    also primes and enhances macrophage killing of tumor cells and
    microorganisms, regulates the release of cytokines and other inflammatory modulators from macrophages, and stimulates pinocytosis （2, 3）. MCSF
    increases during
    pregnancy to support implantation and growth of the decidua and placenta （5）. Sources of MCSF
    include fibroblasts, activated macrophages, endometrial secretory
    epithelium, bone marrow stromal cells and activated endothelial cells （1 5）.
    The MCSF
    receptor （cfms）
    transduces its pleotropic effects and mediates its
    endocytosis. MCSF
    mRNAs of various sizes occur （3 9）.
    Full length mouse MCSF
    transcripts encode a 520 amino acid （aa） type I transmembrane （TM） protein with
    a 462 aa extracellular region, a 21 aa TM domain, and a 37 aa cytoplasmic tail that forms a 140 kDa covalent dimer. Differential processing produces two
    proteolytically cleaved, secreted dimers. One is an Nand
    Oglycosylated
    86 kDa dimer, while the other is modified by both glycosylation and chondroitinsulfate
    proteoglycan （PG） to generate a 200 kDa subunit. Although PGmodified
    MCSF
    can circulate, it may be immobilized by attachment to type V collagen （8）. Shorter
    transcripts encode M CSF
    that lacks cleavage and PG sites and produces an Nglycosylated
    68 kDa TM dimer and a slowly produced 44 kDa secreted dimer （7）.
    Although forms may vary in activity and halflife,
    all contain the Nterminal
    150 aa portion that is necessary and sufficient for interaction with the M CSF
    receptor
    （10, 11）. The first 229 aa of mature mouse MCSF
    shares 87%, 83%, 82% and 81% aa identity with corresponding regions of rat, dog, cow and human MCSF,
    respectively （12, 13）. Human MCSF
    is active in the mouse, but mouse MCSF
    is reported to be speciesspecific

    Recombinant Mouse M-CSF巨噬细胞集落刺激因子

     

    上海金畔R&D一级代理咨询 

    KPL Anti-Mouse IgM （mu） Antibody074-1803

    发表于由

    KPL Anti-Mouse IgM （mu） Antibody

    • 产品型号：  074-1803
    • 简单描述
    • KPL Anti-Mouse IgM （mu） AntibodyHuman Serum Adsorbed and Peroxidase labeled
    详细介绍

    KPL Anti-Mouse IgM （mu） Antibody

    Affinity purified polyclonal antibody to Mouse IgM heavy chains （mu） made in Goat and labeled with horseradish peroxidase. Product has been cross-adsorbed to human serum to minimize cross-reactivity to human immunoglobulin. Product is in lyophilized form. Each lot is tested to assure specificity and lot-to-lot consistency using KPL's in-house ELISA assay.

     

     

    cDNA Library,Mouse Brain酶试剂盒Takara

    发表于由

    cDNA Library,Mouse Brain
    品牌 Code No. 产品名称 包装量 价格（元） 说明书 数量
    Takara 9528 cDNA Library,Mouse Brain 5 μg ￥6,008 cDNA Library,Mouse Brain cDNA Library,Mouse Brain cDNA Library,Mouse Brain
    收藏产品 加入购物车
     
    □ 浓度  
         200 μg/ml  
    □ 贮存溶液  
         Tris-HCl (pH 8.0) 10 mM
         EDTA 1 mM
     
    ■ 制品说明
    本系列制品是基于Gubler and Hoffman方法 (Gene (1983) 25:263-269)， 由各种Poly(A)+ RNA制作的质粒DNA型cDNA文库。合成cDNA片段时，使用了含有限制酶Not I位点的Oligo (dT)18 Linker Primer和BamH I (Bgl II)-Sma I 接头，通过这两个限制酶位点，使cDNA片段定向克隆到载体中。克隆前，做了短片段的分离处理，300 bp以下的片段大部分被去除掉。初级文库构建完成后，采用了固体平板培养法，仅对初级文库进行一次扩增，然后使用质粒提取试剂盒，从扩增文库的菌体中提取质粒，调整得到扩增的质粒cDNA文库。使用这种方法得到的扩增文库，尽可能保持了各种cDNA片段的克隆在初级文库中所占的比例。
     
    ■ 保存
    -20℃。
     
    ■ cDNA文库使用的载体和多克隆位点
    本cDNA文库制品使用的载体为pAP3neo，含有SV40启动子，可以在哺乳动物细胞中进行表达。同时，该载体含有ssDNA生成的必要f1 ori、也含有便于RNA合成的T7及T3 RNA聚合酶启动子。
    GenBank Accession No.AB003468
    1. cDNA片段克隆在pAP3neo载体的Bgl II位点和Not I位点之间。
    * 由于cDNA片段合成时，使用了BamH I (Bgl II)-Sma I接头，克隆后，载体的Bgl II 位点失效，不能再被Bgl II酶切开。
    2. Xba I 位点受dam methylase的影响。
     
    ■ 用途
    对未知或已知的cDNA进行PCR筛选。
     
    ■ pAP3neo载体结构图
    cDNA Library,Mouse Brain
     
     

    页面更新：2021-02-22 14:17:23

    cDNA Library,Mouse Heart酶试剂盒Takara

    发表于由

    cDNA Library,Mouse Heart
    品牌 Code No. 产品名称 包装量 价格（元） 说明书 数量
    Takara 9529 cDNA Library,Mouse Heart 5 μg ￥6,008 cDNA Library,Mouse Heart cDNA Library,Mouse Heart cDNA Library,Mouse Heart
    收藏产品 加入购物车
     
    □ 浓度  
         200 μg/ml  
    □ 贮存溶液  
         Tris-HCl (pH 8.0) 10 mM
         EDTA 1 mM
     
    ■ 制品说明
    本系列制品是基于Gubler and Hoffman方法 (Gene (1983) 25:263-269)， 由各种Poly(A)+ RNA制作的质粒DNA型cDNA文库。合成cDNA片段时，使用了含有限制酶Not I位点的Oligo (dT)18 Linker Primer和BamH I (Bgl II)-Sma I 接头，通过这两个限制酶位点，使cDNA片段定向克隆到载体中。克隆前，做了短片段的分离处理，300 bp以下的片段大部分被去除掉。初级文库构建完成后，采用了固体平板培养法，仅对初级文库进行一次扩增，然后使用质粒提取试剂盒，从扩增文库的菌体中提取质粒，调整得到扩增的质粒cDNA文库。使用这种方法得到的扩增文库，尽可能保持了各种cDNA片段的克隆在初级文库中所占的比例。
     
    ■ 保存
    -20℃。
     
    ■ cDNA文库使用的载体和多克隆位点
    本cDNA文库制品使用的载体为pAP3neo，含有SV40启动子，可以在哺乳动物细胞中进行表达。同时，该载体含有ssDNA生成的必要f1 ori、也含有便于RNA合成的T7及T3 RNA聚合酶启动子。
    GenBank Accession No.AB003468
    1. cDNA片段克隆在pAP3neo载体的Bgl II位点和Not I位点之间。
    * 由于cDNA片段合成时，使用了BamH I (Bgl II)-Sma I接头，克隆后，载体的Bgl II 位点失效，不能再被Bgl II酶切开。
    2. Xba I 位点受dam methylase的影响。
     
    ■ 用途
    对未知或已知的cDNA进行PCR筛选。
     
    ■ pAP3neo载体结构图
    cDNA Library,Mouse Heart
     
     

    页面更新：2020-07-28 13:58:53

    cDNA Library,Mouse Kidney酶试剂盒Takara

    发表于由

    cDNA Library,Mouse Kidney
    品牌 Code No. 产品名称 包装量 价格（元） 说明书 数量
    Takara 9530 cDNA Library,Mouse Kidney 5 μg ￥6,008 cDNA Library,Mouse Kidney cDNA Library,Mouse Kidney cDNA Library,Mouse Kidney
    收藏产品 加入购物车
     
    □ 浓度  
         200 μg/ml  
    □ 贮存溶液  
         Tris-HCl (pH 8.0) 10 mM
         EDTA 1 mM
     
    ■ 制品说明
    本系列制品是基于Gubler and Hoffman方法 (Gene (1983) 25:263-269)， 由各种Poly(A)+ RNA制作的质粒DNA型cDNA文库。合成cDNA片段时，使用了含有限制酶Not I位点的Oligo (dT)18 Linker Primer和BamH I (Bgl II)-Sma I 接头，通过这两个限制酶位点，使cDNA片段定向克隆到载体中。克隆前，做了短片段的分离处理，300 bp以下的片段大部分被去除掉。初级文库构建完成后，采用了固体平板培养法，仅对初级文库进行一次扩增，然后使用质粒提取试剂盒，从扩增文库的菌体中提取质粒，调整得到扩增的质粒cDNA文库。使用这种方法得到的扩增文库，尽可能保持了各种cDNA片段的克隆在初级文库中所占的比例。
     
    ■ 保存
    -20℃。
     
    ■ cDNA文库使用的载体和多克隆位点
    本cDNA文库制品使用的载体为pAP3neo，含有SV40启动子，可以在哺乳动物细胞中进行表达。同时，该载体含有ssDNA生成的必要f1 ori、也含有便于RNA合成的T7及T3 RNA聚合酶启动子。
    GenBank Accession No.AB003468
    1. cDNA片段克隆在pAP3neo载体的Bgl II位点和Not I位点之间。
    * 由于cDNA片段合成时，使用了BamH I (Bgl II)-Sma I接头，克隆后，载体的Bgl II 位点失效，不能再被Bgl II酶切开。
    2. Xba I 位点受dam methylase的影响。
     
    ■ 用途
    对未知或已知的cDNA进行PCR筛选。
     
    ■ pAP3neo载体结构图
    cDNA Library,Mouse Kidney
     

    页面更新：2020-07-28 14:09:55

    cDNA Library,Mouse Liver酶试剂盒Takara

    发表于由

    cDNA Library,Mouse Liver
    品牌 Code No. 产品名称 包装量 价格（元） 说明书 数量
    Takara 9531 cDNA Library,Mouse Liver 5 μg ￥6,008 cDNA Library,Mouse Liver cDNA Library,Mouse Liver cDNA Library,Mouse Liver
    收藏产品 加入购物车
     
    □ 浓度  
         200 μg/ml  
    □ 贮存溶液  
         Tris-HCl (pH 8.0) 10 mM
         EDTA 1 mM
     
    ■ 制品说明
    本系列制品是基于Gubler and Hoffman方法 (Gene (1983) 25:263-269)， 由各种Poly(A)+ RNA制作的质粒DNA型cDNA文库。合成cDNA片段时，使用了含有限制酶Not I位点的Oligo (dT)18 Linker Primer和BamH I (Bgl II)-Sma I 接头，通过这两个限制酶位点，使cDNA片段定向克隆到载体中。克隆前，做了短片段的分离处理，300 bp以下的片段大部分被去除掉。初级文库构建完成后，采用了固体平板培养法，仅对初级文库进行一次扩增，然后使用质粒提取试剂盒，从扩增文库的菌体中提取质粒，调整得到扩增的质粒cDNA文库。使用这种方法得到的扩增文库，尽可能保持了各种cDNA片段的克隆在初级文库中所占的比例。
     
    ■ 保存
    -20℃。
     
    ■ cDNA文库使用的载体和多克隆位点
    本cDNA文库制品使用的载体为pAP3neo，含有SV40启动子，可以在哺乳动物细胞中进行表达。同时，该载体含有ssDNA生成的必要f1 ori、也含有便于RNA合成的T7及T3 RNA聚合酶启动子。
    GenBank Accession No.AB003468
    1. cDNA片段克隆在pAP3neo载体的Bgl II位点和Not I位点之间。
    * 由于cDNA片段合成时，使用了BamH I (Bgl II)-Sma I接头，克隆后，载体的Bgl II 位点失效，不能再被Bgl II酶切开。
    2. Xba I 位点受dam methylase的影响。
     
    ■ 用途
    对未知或已知的cDNA进行PCR筛选。
     
    ■ pAP3neo载体结构图
    cDNA Library,Mouse Liver
     
     

    页面更新：2021-02-22 14:26:37

    cDNA Library,Mouse Lung酶试剂盒Takara

    发表于由

    cDNA Library,Mouse Lung
    品牌 Code No. 产品名称 包装量 价格（元） 说明书 数量
    Takara 9532 cDNA Library,Mouse Lung 5 μg ￥6,008 cDNA Library,Mouse Lung cDNA Library,Mouse Lung cDNA Library,Mouse Lung
    收藏产品 加入购物车
     
    □ 浓度  
         200 μg/ml  
    □ 贮存溶液  
         Tris-HCl (pH 8.0) 10 mM
         EDTA 1 mM
     
    ■ 制品说明
    本系列制品是基于Gubler and Hoffman方法 (Gene (1983) 25:263-269)， 由各种Poly(A)+ RNA制作的质粒DNA型cDNA文库。合成cDNA片段时，使用了含有限制酶Not I位点的Oligo (dT)18 Linker Primer和BamH I (Bgl II)-Sma I 接头，通过这两个限制酶位点，使cDNA片段定向克隆到载体中。克隆前，做了短片段的分离处理，300 bp以下的片段大部分被去除掉。初级文库构建完成后，采用了固体平板培养法，仅对初级文库进行一次扩增，然后使用质粒提取试剂盒，从扩增文库的菌体中提取质粒，调整得到扩增的质粒cDNA文库。使用这种方法得到的扩增文库，尽可能保持了各种cDNA片段的克隆在初级文库中所占的比例。
     
    ■ 保存
    -20℃。
     
    ■ cDNA文库使用的载体和多克隆位点
    本cDNA文库制品使用的载体为pAP3neo，含有SV40启动子，可以在哺乳动物细胞中进行表达。同时，该载体含有ssDNA生成的必要f1 ori、也含有便于RNA合成的T7及T3 RNA聚合酶启动子。
    GenBank Accession No.AB003468
    1. cDNA片段克隆在pAP3neo载体的Bgl II位点和Not I位点之间。
    * 由于cDNA片段合成时，使用了BamH I (Bgl II)-Sma I接头，克隆后，载体的Bgl II 位点失效，不能再被Bgl II酶切开。
    2. Xba I 位点受dam methylase的影响。
     
    ■ 用途
    对未知或已知的cDNA进行PCR筛选。
     
    ■ pAP3neo载体结构图
    cDNA Library,Mouse Lung
     
     

    页面更新：2020-07-28 14:19:12