RNAlater Stabilization Reagent可稳定和保护在收集和储存样本时的RNA表达谱（参见”RNAlater Reagent prevents degradation of mRNA in tissues”）。RNAlater技术对RNA的立即保护，可确保下游分析真实地反映整个组织的表达谱（参见”RNAlater TissueProtect Tubes prevent RNA changes in tissues”）。样本可长期保存，无RNA降解风险，甚至经多次冻融后也无影响。在从多种条件下保存的RNAlater稳定样本中纯化得到的RNA，适用于northern印迹和RT-PCR等下游应用（参见”Stable RNA in tissues: different temperatures and freeze–thaw cycles”）。
注意：使用手动QIAamp DNA Blood Kit和QIAGEN Blood & Cell Culture DNA Kit的用户应该用7 ml蒸馏水重悬每个瓶中的QIAGEN Protease。
注意：使用QIAamp DNA Blood BioRobot 9604 Kit的用户应该用10 ml蒸馏水重悬每个瓶中的QIAGEN Protease。
注意：QIAGEN蛋白酶不可与DNeasy Tissue、DNeasy 96 Tissue及QIAamp DNA Mini Kit中的ATL缓冲液同时使用。在SDS浓度大于0.5%、肌氨酰浓度大于1%、或有高浓度的其他去污剂时，EDTA的浓度必须小于8 mM，这样才能在延长的孵育时间内达到an quan活性。
LIFE ambion trizol LS 200ml货号10296-028规格：200mlTRIzol® LS Reagent 是苯酚和异硫氰酸胍的即用型单相溶液，设计用于从人、动物、植物、酵母、细菌和病毒来源的液体样品中分离总 RNA。 在样品匀浆或裂解过程中，TRIzol® LS Reagent 一方面保持 RNA 的完整性，另一方面破坏细胞并裂解细胞组分。
详细介绍
LIFE ambion trizol LS 200ml货号10296-028
TRIzol® LS 试剂分离的总 RNA 不含蛋白质和 DNA 杂质。 可用于 Northern blot 分析，点印迹杂交，多聚 (A)+ 选择，体外翻译，RNA 酶保护分析，RT-PCR 和分子克隆。 对于 RT-PCR，当一个外显子中存在两个引物时， 建议用扩增级 DNase I（商品目录号 18068-015）处理分离的 RNA。
TRIzol® LS Reagent 的特点： • 设计用于液体样品，例如血清和病毒制品 • 使用 TRIzol® LS 试剂与液体样品的比值为 3:1 • 有助于多种大分子或小分子 RNA 的分离
LIFE ambion trizol LS 200ml货号10296-028
述
TRIzol® LS Reagent is a complete, ready-to-use reagent optimized for the isolation of high-quality total RNA, or the simultaneous isolation of RNA, DNA, and protein from a variety of liquid samples. This monophasic solution of phenol and guanidine isothiocyanate is designed to isolate separate fractions of RNA, DNA, and proteins from liquid samples of human, animal, plant, yeast, bacterial, and viral origin, typically within one hour.
• Formulated for use with liquid samples such as serum and virus preparations • Facilitates recovery of RNA, DNA, and protein from a single liquid sample • Offers excellent lysis capability, even with difficult biological fluids
Reliably Purify RNA from Multiple Sample Sources TRIzol® LS Reagent is designed for processing a variety of liquid samples of up to 0.25 ml in volume. TRIzol® LS Reagent differs from the standard TRIzol® Reagent in concentration, which permits larger samples to be processed. Just as with the standard TRIzol® Reagent, the integrity of resulting RNA preparations is maintained by the highly effective inhibition of RNase activity during sample homogenization. The simplicity of the TRIzol® LS Reagent method allows simultaneous processing of a large number of samples. The entire procedure can be completed in 1 hour. Total RNA isolated by TRIzol® LS Reagent is free of protein and DNA contamination.
Formulated to Serve Multiple Isolations TRIzol® LS Reagent allows you to perform sequential precipitation of RNA, DNA, and proteins from a single sample. After homogenizing the sample with TRIzol® LS Reagent, chloroform is added, and the homogenate is allowed to separate into a clear upper aqueous layer (containing RNA), an interface, and a red lower organic layer (containing the DNA and proteins). RNA is precipitated from the aqueous layer with isopropanol. DNA is precipitated from the aqueous/organic interface with ethanol. Protein is precipitated from the phenol-ethanol layer by isopropanol precipitation. The precipitated RNA, DNA, or protein is washed to remove impurities, and then resuspended for use in downstream applications.
Purified Products are Ideal for Use with a Variety of Applications Isolated RNA can be used in real-time quantitative PCR (qPCR), northern blot analysis, dot blot hybridization, poly-(A)+ selection, In vitro translation, RNase protection assays, and molecular cloning. Isolated DNA can be used in PCR, restriction enzyme digestion, and Southern blots. Isolated protein can be used for western blots, recovery of some enzymatic activity, and someimmunoprecipitation.
Invitrogen TRIzol LS Reagent 100ml货号10296-010规格：100mlTRIzol® LS Reagent 特点：• 设计用于液体样品，例如血清和病毒制品• 使用 TRIzol® LS 试剂与液体样品的比值为 3:1• 有助于多种大分子或小分子 RNA 的分离
详细介绍
Invitrogen TRIzol LS Reagent 100ml货号10296-010
TRIzol® LS Reagent 是苯酚和异硫氰酸胍的即用型单相溶液，设计用于从人、动物、植物、酵母、细菌和病毒来源的液体样品中分离总 RNA。 在样品匀浆或裂解过程中，TRIzol® LS Reagent 一方面保持 RNA 的完整性，另一方面破坏细胞并裂解细胞组分。
TRIzol® LS 试剂分离的总 RNA 不含蛋白质和 DNA 杂质。 可用于 Northern blot 分析，点印迹杂交，多聚 (A)+ 选择，体外翻译，RNA 酶保护分析，RT-PCR 和分子克隆。 对于 RT-PCR，当一个外显子中存在两个引物时， 建议用扩增级 DNase I（商品目录号 18068-015）处理分离的 RNA
描述
TRIzol® LS Reagent is a complete, ready-to-use reagent optimized for the isolation of high-quality total RNA, or the simultaneous isolation of RNA, DNA, and protein from a variety of liquid samples. This monophasic solution of phenol and guanidine isothiocyanate is designed to isolate separate fractions of RNA, DNA, and proteins from liquid samples of human, animal, plant, yeast, bacterial, and viral origin, typically within one hour.
• Formulated for use with liquid samples such as serum and virus preparations • Facilitates recovery of RNA, DNA, and protein from a single liquid sample • Offers excellent lysis capability, even with difficult biological fluids
为什么要选择Mediatech （CorningCellGro®）? Mediatech遵循美国食品及药物管理局（FDA）认证的cGMP标准 Mediatech通过ISO13485:2003认证 Mediatech符合FDA的无菌保证水平（SAL） 采用IBCUS（Integrated Bar Code User System）确保产品配方的准确性 Mediatech保证产品低内毒素水平，无支原体污染
35-076-CV
详细参数：
– Complete Certificate of Analysis available for each production lot
– Produced under the highest industry standards to ensure superior results
– This is a competitively priced, high quality serum
Fetal Bovine Serum (FBS) is the most common supplement used in cell culture. Its use at specific concentrations provides many compounds that have been shown to satisfy specific metabolic requirements for the culture of cells, including various growth promoting factors, along with a variety of undefined components. This FBS is from USDA-approved countries outside of the United States.
SCHOTT DURAN(肖特)蓝盖瓶具有显著特性的DURAN实验室玻璃瓶已赢得市场好评多年。经过不断发展和提高, DURAN GROUP在原有产品的基础上推出了一些了设计精良的优质产品。其产品在贮存、包装及配置应用方面的*性、可靠性及灵活性使得DURAN实验室玻璃瓶成 为化学、医药及生物科学领域的标准,DURAN实验室玻璃早已广泛应用于各地的实验室，产品种类达4,000多种。
