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Products > Crystallization Plates, Hardware & Accessories > Micro-Bridges > Micro-Bridges
Micro-Bridges
应用
- Sitting drop crystallization
- Heavy atom soaks
- Seeding
- 坐滴结晶
重原子浸泡
播种
特征
- Perform sitting drop experiments in 24 well hanging drop plates
- Removable
- 在 24 孔悬挂滴板中进行坐滴实验
可拆卸的
描述
Micro-Bridges are small devices in the shape of a bridge that are designed to carry out sitting drop vapor diffusion crystallization when placed in a VDX™ Plate or Linbro® Plate. A single Micro-Bridge fits neatly into the reservoir of a standard 24 well VDX Plate for a sitting drop crystallization experiment. Once placed inside the wells, Micro-Bridges are stable and there is no need to stick them to the wells with grease or adhesive. It is therefore possible to transfer them to other wells during or after a crystallization experiment.
Why sitting drop? Placing the droplet in the indentation greatly reduces the risk of losing the protein by accident. Crystallization can be carried out in the presence of detergents and organic solvents which are compatible with polystyrene (such as MPD, iso-propanol, and ethanol). The protein drop is less affected by condensation problems. Soaking and seeding experiments can be carried out easily. Crystals can be transported more securely. Larger drop volumes can be used. Micro-Bridges have a concave indentation in the top surface of the bridge which holds the sample droplet during a crystallization experiment and prevents the droplet from spreading over a large area. Made from polystyrene, these parts are highly transparent and suitable for most crystallizations. The surface of the indentation is highly polished to facilitate the visual inspection of the drops under a microscope. The maximum drop volume for the Micro-Bridge is 35 µl. Reservoirs can be sealed with plain cover slides and vacuum grease or clear sealing tape.
Approximate dimensions: Height: 12 mm Length: 14 mm Width: 7 mm
微桥是桥形的小型设备，设计用于在放置在 VDX™ 板或 Linbro® 板中时进行坐滴蒸汽扩散结晶。单个 Micro-Bridge 可巧妙地装入标准 24 孔 VDX 板的储液器中，用于坐滴结晶实验。一旦放置在孔内，Micro-Bridges 就很稳定，无需用油脂或粘合剂将它们粘在孔上。因此，可以在结晶实验期间或之后将它们转移到其他孔中。
为什么要坐下来？将液滴放入压痕中可大大降低意外丢失蛋白质的风险。可以在与聚苯乙烯相容的去污剂和有机溶剂（如 MPD、异丙醇和乙醇）存在下进行结晶。蛋白质下降受冷凝问题的影响较小。可以轻松进行浸泡和播种实验。水晶可以更安全地运输。可以使用更大的液滴体积。微桥在桥的顶部表面有一个凹形压痕，用于在结晶实验期间固定样品液滴并防止液滴在大面积上扩散。这些部件由聚苯乙烯制成，高度透明，适用于大多数结晶。压痕表面经过高度抛光，便于在显微镜下目视检查液滴。 Micro-Bridge 的最大滴体积为 35 µl。可以使用普通盖玻片和真空润滑脂或透明密封胶带密封储液罐。
大概尺寸： 高度：12 毫米 长度：14 毫米 宽度：7 毫米
HR3-310/HR3-312 Micro-Bridges
CAT NO
HR3-310
NAME
DESCRIPTION
100 pack
PRICE
$86.00
CAT NO
HR3-312
NAME
DESCRIPTION
400 pack
PRICE
$297.00
支持材料
HR3-310 HR3-312 Micro-Bridges User Guide参考
1. Structure of aluminum-bound ovotransferrin at 2.15 angstrm resolution. K, Mizutani, B. Mikami, S. Aibara, and M. Hirose. Acta Cryst (2005) D61, 1636-1642.
1. 2.15 埃分辨率下铝结合卵转铁蛋白的结构。 K、Mizutani、B. Mikami、S. Aibara 和 M. Hirose。 水晶学报 (2005) D61, 1636-1642。
