Wide partition walls between wells improve sealing
Developed in conjunction with the MRC Laboratory of Molecular Biology in Cambridge, United Kingdom
UV compatible (UVP)
Description
MRC Maxi 48-Well Crystallization Plate for Automated Optimization
The MRC Maxi optimization plate is a breakthrough for macromolecular crystallization presented in a 48 well format. Offering easy to automate crystallization optimization with large sitting-drops, the new MRC Maxi Crystallization plate is the perfect solution. Manufactured by Swissci AG, the plate offers an SBS format while providing 48 wells. MRC Maxi is intended for large drops and is compatible both with standard robotic systems as well as manual pipetting.
The plate was developed at the MRC Laboratory of Molecular Biology (Cambridge, UK) in collaboration with Jan Löwe and Fabrice Gorrec. It is a result of many years of experience in successful robotic high-throughput crystallization and complements the original MRC crystallization plate, which is intended for smaller drop volumes and higher throughput during screening.
Drop volumes of up to 10 µl are possible. The 9 mm standard distance between wells is preserved, enabling the use of multi-channel manual pipettes and robotic liquid handlers, making MRC Maxi one of the most automation-friendly optimization plates on the market.
MRC Maxi is covered by global intellectual property and design registration as are the Swissci AG MRC 2 lens 96 well plates. Several breakthrough features of the original MRC plate have been maintained. Wells are labeled individually. Drops are raised for easy access during crystal retrieval. MRC Maxi uses the same proprietary polymer specially selected for the purpose of UV light visualization and the material used keeps through-plastic evaporation to a minimum. Well shapes are spherical but shallow. The
MRC Maxi Crystallization plate offers unique properties that make it the ideal choice for microliter-sized optimization experiments and is made from UV compatible UVP.
The advantages of the MRC Maxi Crystallization plate – in brief
Easy Crystal Retrieval • Raised wide wells make the crystal mounting especially easy.
Easy Viewing • The wells are wide conical. • Each well has a micro lens for perfect illumination. • Micro numbering readable under the microscope for each well. • The optically superior polymer (UVP) is UV transmissible.
Better Sealing • Wide partition walls between the wells give plenty of area for good sealing with tape.
Very Rigid, Automation-Friendly Plate Design • The UVP polymer reduces through-plastic evaporation to a minimum.
SBS Standard • The plates are designed to the SBS standard and are compatible with all common holders. • 9 mm distance from well-to-well within columns, 18 mm distance within rows.
图2. 使用Clontech慢病毒纯化试剂盒可获得高产量和高纯度。使用我们的高滴度慢病毒包装系统制备并纯化慢病毒。Panel A. 为了确定病毒产量,通过qRT-PCR(RNA拷贝数)或流式细胞仪/荧光(IFU)测定纯化前和纯化后的慢病毒滴度,展示数据为七组实验的平均值。Panel B. 使用Lenti-X或基于过滤器的纯化系统(Vendor M和Vendor S)制备等量的纯化后慢病毒(1×105 IFU)进行SDS-PAGE和银染。与其它两种基于过滤器系统所制备的样品相比,在相同IFU的情况下Lenti-X样品含有明显更少的污染蛋白质。