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takara荧光实时定量qPCR试剂RT Master Mix反转录RR036A

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takara荧光实时定量qPCR试剂RT Master Mix反转录

  • 产品型号：  RR036A
  • 简单描述
  • takara荧光实时定量qPCR试剂RT Master Mix反转录本制品是2 Step Real Time RT-PCR用的理想反转录反应试剂。5×PrimeScript RT Master Mix中含有定量RT-PCR的反转录反应所需的所有试剂 （PrimeScript RTase、RNase Inhibitor、Random 6 mers、Oligo dT Primer、dNTP Mix
详细介绍

takara荧光实时定量qPCR试剂RT Master Mix反转录

5×PrimeScript RT Master Mix (Perfect Real Time)*1 400 μl
RNase Free dH2O 1 ml ×2
EASY Dilution (for Real Time PCR)*2 1 ml
 
*1 含有PrimeScript RTase、RNase Inhibitor、Random 6 mers、Oligo dT Primer、dNTP Mixture、反应Buffer (含有Mg2+) 。
*2 制作标准曲线时梯度稀释cDNA或RNA标准品的稀释液。模板cDNA或RNA如果用水或TE Buffer稀释时，由于受Microtube吸附作用等的影响，往往不能准确地进行稀释，导致实验结果精度降低。使用本制品时，即使稀释至低浓度也能够进行准确地稀释，容易在宽广范围内获得准确定量的标准曲线。EASY Dilution (for Real Time PCR) 也可以单独购买 (Code No.: 9160/9160Q)。 
注意：EASY Dilution请与本公司Real Time PCR试剂组合使用，对于其他公司的同类制品的适用性本公司尚未进行确认。 

takara荧光实时定量qPCR试剂RT Master Mix反转录

试剂盒特长
1. 制品为预先混好的Premix形式，只需加入模板RNA和水便可以开始反应。
2. 可以在较短时间内高效合成Real Time PCR用cDNA，是进行2 Step Real Time RT-PCR反应的理想试剂。
3. 使用了Random 6 mers和Oligo dT Primer 2种反转录引物，可以合成适合Real Time PCR用cDNA。 
4. Real-time RT PCR定量需要建立标准曲线，建立标准曲线的条件就是需要将总RNA和反转录cDNA稀释到较低的浓度。如果用水或TE Buffer稀释时，由于模板浓度低不稳定，因而会缩小曲线范围，结果精度降低。本制品中附加了标准曲线制作用稀释液EASY Dilution (for Real Time PCR)，将Total RNA或cDNA稀释至低浓度时也能够进行准确稀释，容易在宽广范围内获得准确定量的标准曲线。
 
 
■ 注意事项
1. 5×PrimeScript RT Master Mix在使用前要小心地离心收集到反应管底部。由于5×PrimeScript RT Master Mix粘度高，用移液枪慢慢反复吸打充分混匀后使用。
2. 分装试剂时务必使用新的枪头 (Tip)，以防止样品间污染。
3. 本制品中已经加入了反转录Primer（Oligo dT Primer 和Random 6 mers），如果要使用Gene Specific Primer进行反转录反应，则不能使用本制品。

罗氏ROCHE faststart universal probe master现货04914058001

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罗氏ROCHE faststart universal probe master现货

  • 产品型号：  04914058001
  • 简单描述
  • 罗氏ROCHE faststart universal probe master现货规格5ml现货供应，*
详细介绍

罗氏ROCHE faststart universal probe master现货

罗氏ROCHE faststart universal probe master现货

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