标签归档：mammalian

Peprotech重组人TGF-β1细胞因子Recombinant Human TGF-β1 （mammalian derived）100-21

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Peprotech重组人TGF-β1细胞因子Recombinant Human TGF-β1 （mammalian derived）

  • 产品型号：  100-21
  • 简单描述
  • Recombinant Human TGF-β1 (mammalian derived)重组人TGF-β1细胞因子，详细产品信息请
详细介绍

 

Recombinant Human TGF-β1 （mammalian derived）

Catalog Number:
100-21
Synonyms:
Transforming Growth Factor-β1, Differentiation inhibiting factor, Cartilage-inducing factor
Description:
The three mammalian isoforms of TGF-β, TGF-β1, β2, β3, signal through the same receptor and elicit similar biological responses. They are multifunctional cytokines that regulate cell proliferation, growth, differentiation and motility as well as synthesis and deposition of the extracellular matrix. They are involved in various physiological processes including embryogenesis, tissue remodeling and wound healing. They are secreted predominantly as latent complexes which are stored at the cell surface and in the extracellular matrix. The release of biologically active TGF-β isoform from a latent complex involves proteolytic processing of the complex and /or induction of conformational changes by proteins such as thrombospondin-1. TGF-β1 is the most abundant isoform secreted by almost every cell type. It was originally identified for its ability to induce phenotypic transformation of fibroblasts and recently it has been implicated in the formation of skin tumors. Human TGF-β1 is a 25.0 kDa protein with each subunit containing 112 amino acid residues, linked by a single disulfide bond.
AA Sequence:
ALDTNYCFSS TEKNCCVRQL YIDFRKDLGW KWIHEPKGYH ANFCLGPCPY IWSLDTQYSK VLALYNQHNP GASAAPCCVP QALEPLPIVY YVGRKPKVEQ LSNMIVRSCK CS
Source:
HEK293 cells
Purity:
Greater than 98% by SDS-PAGE gel and HPLC analyses.
Biological Activity:
The ED50 was determined by TGF-beta1’s ability to inhibit the mouse IL-4-dependent proliferation of mouse HT-2 cells is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 107 units/mg.
 
 
 
 
Cross Reactivity:
Chicken, Cow, Dog, Frog, Monkey, Mouse, Pig, Rabbit, Rat, Human, Mink, Donkey, N/A
Research Interests:
Peprotech重组人TGF-β1细胞因子Recombinant Human TGF-β1 （mammalian derived）100-21 Angiogenesis/ Cardiovascular Peprotech重组人TGF-β1细胞因子Recombinant Human TGF-β1 （mammalian derived）100-21 Apoptosis
Peprotech重组人TGF-β1细胞因子Recombinant Human TGF-β1 （mammalian derived）100-21 Cancer Peprotech重组人TGF-β1细胞因子Recombinant Human TGF-β1 （mammalian derived）100-21 Immune System
Peprotech重组人TGF-β1细胞因子Recombinant Human TGF-β1 （mammalian derived）100-21 Inflammation Peprotech重组人TGF-β1细胞因子Recombinant Human TGF-β1 （mammalian derived）100-21 Proliferation
Peprotech重组人TGF-β1细胞因子Recombinant Human TGF-β1 （mammalian derived）100-21 Stem Cells & Differentiation Peprotech重组人TGF-β1细胞因子Recombinant Human TGF-β1 （mammalian derived）100-21 TGF-β Superfamily
Peprotech重组人TGF-β1细胞因子Recombinant Human TGF-β1 （mammalian derived）100-21 Wound Healing Peprotech重组人TGF-β1细胞因子Recombinant Human TGF-β1 （mammalian derived）100-21 Allergy
 
 
Country of Origin:
USA

Peprotech重组人TGF-β1细胞因子Recombinant Human TGF-β1 （mammalian derived）100-21

发表于由

Peprotech重组人TGF-β1细胞因子Recombinant Human TGF-β1 （mammalian derived）

  • 产品型号：  100-21
  • 简单描述
  • Recombinant Human TGF-β1 (mammalian derived)重组人TGF-β1细胞因子，详细产品信息请
详细介绍

 

Recombinant Human TGF-β1 （mammalian derived）

Catalog Number:
100-21
Synonyms:
Transforming Growth Factor-β1, Differentiation inhibiting factor, Cartilage-inducing factor
Description:
The three mammalian isoforms of TGF-β, TGF-β1, β2, β3, signal through the same receptor and elicit similar biological responses. They are multifunctional cytokines that regulate cell proliferation, growth, differentiation and motility as well as synthesis and deposition of the extracellular matrix. They are involved in various physiological processes including embryogenesis, tissue remodeling and wound healing. They are secreted predominantly as latent complexes which are stored at the cell surface and in the extracellular matrix. The release of biologically active TGF-β isoform from a latent complex involves proteolytic processing of the complex and /or induction of conformational changes by proteins such as thrombospondin-1. TGF-β1 is the most abundant isoform secreted by almost every cell type. It was originally identified for its ability to induce phenotypic transformation of fibroblasts and recently it has been implicated in the formation of skin tumors. Human TGF-β1 is a 25.0 kDa protein with each subunit containing 112 amino acid residues, linked by a single disulfide bond.
AA Sequence:
ALDTNYCFSS TEKNCCVRQL YIDFRKDLGW KWIHEPKGYH ANFCLGPCPY IWSLDTQYSK VLALYNQHNP GASAAPCCVP QALEPLPIVY YVGRKPKVEQ LSNMIVRSCK CS
Source:
HEK293 cells
Purity:
Greater than 98% by SDS-PAGE gel and HPLC analyses.
Biological Activity:
The ED50 was determined by TGF-beta1’s ability to inhibit the mouse IL-4-dependent proliferation of mouse HT-2 cells is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 107 units/mg.
 
 
 
 
Cross Reactivity:
Chicken, Cow, Dog, Frog, Monkey, Mouse, Pig, Rabbit, Rat, Human, Mink, Donkey, N/A
Research Interests:
Peprotech重组人TGF-β1细胞因子Recombinant Human TGF-β1 （mammalian derived）100-21 Angiogenesis/ Cardiovascular Peprotech重组人TGF-β1细胞因子Recombinant Human TGF-β1 （mammalian derived）100-21 Apoptosis
Peprotech重组人TGF-β1细胞因子Recombinant Human TGF-β1 （mammalian derived）100-21 Cancer Peprotech重组人TGF-β1细胞因子Recombinant Human TGF-β1 （mammalian derived）100-21 Immune System
Peprotech重组人TGF-β1细胞因子Recombinant Human TGF-β1 （mammalian derived）100-21 Inflammation Peprotech重组人TGF-β1细胞因子Recombinant Human TGF-β1 （mammalian derived）100-21 Proliferation
Peprotech重组人TGF-β1细胞因子Recombinant Human TGF-β1 （mammalian derived）100-21 Stem Cells & Differentiation Peprotech重组人TGF-β1细胞因子Recombinant Human TGF-β1 （mammalian derived）100-21 TGF-β Superfamily
Peprotech重组人TGF-β1细胞因子Recombinant Human TGF-β1 （mammalian derived）100-21 Wound Healing Peprotech重组人TGF-β1细胞因子Recombinant Human TGF-β1 （mammalian derived）100-21 Allergy
 
 
Country of Origin:
USA

Real Time PCR Mammalian DNA Detection Kit酶试剂盒Takara

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Real Time PCR Mammalian DNA Detection Kit
品牌 Code No. 产品名称 包装量 价格（元） 说明书 数量
Takara RR915 Real Time PCR Mammalian DNA Detection Kit 50 Rxns 询价 Real Time PCR Mammalian DNA Detection Kit Real Time PCR Mammalian DNA Detection Kit Real Time PCR Mammalian DNA Detection Kit
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■ 制品内容 (25 μl反应×50 次量)
2X Premix for Mammalian*1 650 μl
Primer Mix for Mammalian*2 50 μl
Probe Mix for Mammalian*3 50 μl
dH2O 1 ml
Control DNA for Mammalian (10 ng/μl) 15 μl
 
*1 2X Premix For Mammalian中含有反应用dNTP Mixture、Buffer、酶等。
*2 Primer Mix for Mammalian中含有扩增用引物及内参照 (Internal Control)。
*3 Probe Mix for Mammalian中含有检测用探针，须避光保存。
 
■ 制品说明
本制品是利用Real Time PCR技术快速检测常见哺乳动物*基因组DNA的试剂盒。采用双标记荧光PCR技术，根据线粒体DNA 12S rRNA基因上哺乳动物与非哺乳动物种间序列差异而研发的鉴定方法，能够对饲料中哺乳动物源性成分 (包括牛、绵羊、山羊、猪、兔、鹿、骆驼、马、驴、狗、猫等) 进行鉴定。应用多色荧光检测技术，对反应液中含有的两种不同荧光染料进行双通道同步检测，在同一反应管内对哺乳动物的12S rRNA基因及内参照 (Internal Control) 同时进行扩增，并通过标记两种不同荧光物质 (FAM、HEX) 的特异性探针进行特异性杂交，实现多色荧光同步检测。其中对内参照反应的检测，可以监控反应是否正常进行，防止假阴性结果。制品中2X Premix for Mammalian已经将DNA聚合酶、反应用Buffer、dNTP等试剂预混在一起。进行实验时，PCR反应液的配制十分简单。PCR反应用DNA聚合酶使用了Hot Start法用DNA聚合酶TaKaRa Ex Taq HS，与Takara精心研制的Real Time PCR用Buffer组合使用，可以有效抑制非特异性的PCR扩增，大大提高了PCR的扩增效率。本检测无需电泳，简单快速。本制品适用于食品、化妆品和饲料等样品中哺乳动物源性成分的鉴别。
本试剂盒的引物探针序列，参考自《GB/T 21103-2007动物源性饲料中哺乳动物源性成分定性检测方法实时荧光PCR方法》。
*：由于检测品种不同，扩增序列可能存在变异，因此某些品种的哺乳动物可能无法被检出。
 
■ 保存
-20℃。
 
■ 注意事项
1. 本试剂盒检测灵敏度高，为了防止污染，实验要分区操作。
① 第一区：反应液配制区。
② 第二区：样品制备区。
③ 第三区：样品添加区及反应检测区。
三个区之间必须进行物理性隔离， 避免人为因素造成污染。
2. 2X Premix for Mammalian制品融解后，请上下颠倒轻轻均匀混合，避免起泡，并经轻微离心后使用。
3. Probe Mix for Mammalian应避光保存。
4. 配制Real Time PCR反应液时应避免强光照射。
5. 反应液的配制、分装请一定使用新的 (无污染的) 枪头、Microtube等，尽量避免污染。
 
 

页面更新：2020-06-18 15:46:33