LIFE lipo3000(脂质体3000)1.5MLL3000-150

LIFE lipo3000(脂质体3000)1.5ML

  • 产品型号:  L3000-150
  • 简单描述
  • LIFE lipo3000(脂质体3000)1.5ML规格:1.5ml英文名:Lipofectamine® 3000 Transfection ReagentZ大有点:转染效率高于lipo2000,毒性低于lipo2000
详细介绍

LIFE lipo3000(脂质体3000)1.5ML

Lipofectamine® 3000 Transfection Reagent

Lipofectamine® 3000转染试剂中包含了我们*的脂质体纳米颗粒技术,能够提供的转染性能,改善相关应用的结果及可重复性。此试剂具有优异的转染效率,并可提高细胞活性,广泛适用于难转染的及常见的细胞种类(例如HEK293和Hela细胞等)

LIFE lipo3000(脂质体3000)1.5ML

特点:

 提供的性能——我们针对难以转染的细胞种类实现了zui高转染效率的试剂
 提高细胞活性——对细胞作用温和,毒性很低
功能多样——同时适用于DNA、RNA及共转染应用

 

Lipofectamine® 3000试剂专门针对难转染的细胞种类进行了优化,能够为广泛的细胞种类提供的转染性能。其可高效转染的细胞类型包括:

 

成纤维细胞(3T3、COS-7) 成肌细胞(C2C12、L6 CRL-1458)
肝细胞(HepG2、HuH7) 红白血病细胞(K562)
乳腺癌(MCF7、Hs578T) 前列腺癌(LNCap)
肺癌细胞(A549、NCI-H460) 骨肉瘤细胞(U-2 OS、Saos-2)
结肠癌细胞(Caco2、SW480) 胰腺癌细胞(PANC-1)
皮肤黑色素瘤细胞(SK-MEL-28)

订购信息:

货号

单位规格

目录价格 (CNY)数量
L3000-001

0.1 mL

562.00

L3000-008

0.75 mL

3,335.00

L3000-015

1.5 mL

5,570.00

L3000-075

5 x 1.5 mL

26,650.00

 

 

 

LIFE RNase ZAP AM9780AM9782

LIFE RNase ZAP AM9780

  • 产品型号:  AM9782
  • 简单描述
  • LIFE RNase ZAP AM9780Reagent Type:RNase Control ReagentForm:Liquid规格:250 mLShipping Condition:Room Temperature
详细介绍

LIFE RNase ZAP AM9780

RNaseZap? RNase Decontamination Solution is a surface decontamination solution that destroys RNases on contact. You simply spray RNaseZap? Solution onto the surface to be decontaminated and rinse it off with RNase-free water.

LIFE RNase ZAP AM9780

eatures of RNaseZap? RNase Decontamination Solution:

? Compley removes RNase contamination from glass and plastic surfaces
? Excels at removing high levels of RNase contamination whereas similar products fail
? Proven effective at removing high concentrations of dried-on RNase A
? Ideal for cleaning work surfaces, pipettors, and equipment that must be RNase-free

Using RNaseZap? Solution
Working with RNA requires that special measures be taken to ensure an RNase-free environment. Even trace quantities of RNase can lead to lower yields from in vitro transcription reactions, degradation during RNA purification protocols, and variable results in RPAs and Northerns. RNaseZap? contains three ingredients active against RNase and has proven to be extremely effective at removing RNase contamination from glassware, plastic surfaces, countertops, and pipettors. It has also been shown to be effective at eliminating RNase contamination from microfuge tubes without inhibiting subsequent enzymatic reactions. Supplied in one 250 mL bottle.

订购信息:

货号: AM9780

Ambion?  相关应用: RNA 纯化 | 实时定量 PCR

AM9780 250ml

AM9782 8*250ML

AM9784 4L

LIFE(invitrogen)RNAiMAX转染试剂 1377813778-030 13778-150

LIFE(invitrogen)RNAiMAX转染试剂 13778

  • 产品型号:  13778-030 13778-150
  • 简单描述
  • LIFE(invitrogen)RNAiMAX转染试剂 13778规格:13778-030 0.3ml 13778-075 0.75ml 13778-150 1.5ml 13778-100 0.1ml 13778-500 15ml
详细介绍

详细LIFE(invitrogen)RNAiMAX转染试剂 13778

Lipofectamine? RNAiMAX转染试剂可在多种细胞类型中获得zui高的转染效率,适用于siRNA介导的基因抑制实验。Lipofectamine? RNAiMAX是一种*的RNAi特异性阳离子脂质体转染试剂,适合将siRNA和miRNA输送至所有细胞类型中。

LIFE(invitrogen)RNAiMAX转染试剂 13778

特点:

简单、高通量的即用型转染
只需将Lipofectamine? RNAiMAX转染试剂与siRNA混合,加入细胞,在孵育所要求的时间后,检测基因抑制效果。Lipofectamine? RNAiMAX转染试剂使用简单、快速且具有很高的转染效率,适用于高通量siRNA转染。在上述应用中,可轻松实现自动化或机器人系统转染条件的设定。

采用Lipofectamine? RNAiMAX转染试剂,您可以实现:
? 超高的转染效率,且所需RNAi浓度低,基因抑制效果更好,非特异性效应极少
? 细胞毒性低、易于优化,跨10倍转染试剂浓度范围均显示了低细胞毒性
? 针对miRNA拮抗剂和模拟物具有出色的转染效率
? 可用于多种细胞类型,是适合各种基因抑制实验的zui通用方法
? 简单快速的实验方案,可获得稳定且可重复的结果

在多种细胞中可获得很高的基因抑制效果
Lipofectamine? RNAiMAX转染试剂可转染各种类型的细胞(图1)。在基因抑制应用中,Lipofectamine? RNAiMAX转染试剂的高效转染提供了很高的基因抑制水平,足以获得成功的结果。

详细信息:

Cell Type: Established Cell Lines, Hard-to-Transfect Cells, Primary Cells, Stem Cells
Sample Type (Specific): Synthetic siRNA
Transfection Technique: Lipid-Based Transfection
Serum Compatible: Yes
Product Size: 0.1 mL
Shipping Condition: Approved for shipment at Room Temperature or on Wet Ice
High Throughput Compatibility: High Throughput-Compatible
Green Features: Sustainable packaging

订购信息:

Lipofectamine? RNAiMAX Transfection Reagent

13778-030  0.3ml
         13778-075 0.75ml 
         13778-150 1.5ml
         13778-100 0.1ml
         13778-500 15ml

LIFE CellTracker CM-DiI – Special PackagingC7000

LIFE CellTracker CM-DiI – Special Packaging

  • 产品型号:  C7000
  • 简单描述
  • LIFE CellTracker CM-DiI – Special PackagingColor: Yellow Platform: Fluorescence Microscope Sub-Cellular Localization: Cell Membranes & Lipids Product Size: 20 x 50 µg Detection Method: Fluor
详细介绍

LIFE CellTracker CM-DiI – Special Packaging
订购信息:

货号

单位规格

目录价格 (CNY) 针对您的报价 (CNY) 库存状态 数量
C-7000 C-7000

20 x 50 μg

3,675.00
C-7001 C-7001

1 mg

3,229.00

LIFE CellTracker CM-DiI – Special Packaging

描述

CellTracker CM-DiI 为 DiI 衍生物,在水中的溶解性比 DiI 要好一些,有利于制备悬浮细胞和固定细胞的染色溶液。 CellTracker CM-DiI 含有能与硫醇反应的氯甲基基团,使染料可以共价结合到细胞硫醇上。 这样,与其它膜染料不同,一些细胞可在所有固定和渗透步骤中保持稳定的标记。 我们还提供 1 mg 瓶装形式的 CellTracker CM-DiI(C-7001)。

Figures

LIFE CellTracker CM-DiI - Special PackagingC7000

LIFE CellTracker CM-DiI - Special PackagingC7000

Brain of a zebrafish embryo. CellTracker CM-DiI.

LIFE CellTracker CM-DiI - Special PackagingC7000

Persistence of lipophilic tracer fluorescence following fixation.

LIFE CellTracker CM-DiI - Special PackagingC7000

A 30 μm–thick section of a zebrafish embryo stained with CellTracker? CM-DiI.

LIFE CellTracker CM-DiI - Special PackagingC7000

Neural somata from a mouse brain section labeled with NeuroTrace? 500/525 green-fluorescent Nissl stain.

LIFE CellTracker CM-DiI - Special PackagingC7000

Neural somata from mouse brain section. NeuroTrace 500/525 green-fluorescent Nissl stain, DAPI and CellTracker CM-DiI.

LIFE CellTracker CM-DiI - Special PackagingC7000

规格

Color: Yellow
Platform: Fluorescence Microscope
Sub-Cellular Localization: Cell Membranes & Lipids
Product Size: 20 x 50 μg
Detection Method: Fluorescent
Excitation?Emission (nm): 553?570
Shipping Condition: Room Temperature

化学结构

未找到结果.

CellTracker?CM-DiI
分子式: C68H105Cl2N3O
分子量: 1051.5
CAS名称/编号: 3H-Indolium, 5-[[[4-(chloromethyl)benzoyl]amino]methyl]-2-[3-(1,3-dihydro-3,3-dimethyl-1-octadecyl-2H-indol-2-ylidene)-1-propenyl]-3,3-dimethyl-1-octadecyl-, chloride 180854-97-1

LIFE CellTracker CM-DiI - Special PackagingC7000

荧光光谱

未找到结果.

DiI
Fluorescence Ex/Em spectra of DiIC<sub>18</sub>(3) (“”DiI””) bound to phospholipid bilayer membranes.

LIFE CellTracker CM-DiI - Special PackagingC7000
Open in SpectraViewer

文档

life CTS TrypLE Select EnzymeA12859

life CTS TrypLE Select Enzyme

  • 产品型号:  A12859
  • 简单描述
  • life CTS TrypLE Select Enzyme规格:100ml
详细介绍

life CTS TrypLE Select Enzyme

Chelators: EDTA
Phenol Red Indicator: No Phenol Red
Classification: Animal Origin-Free
Enzyme Stability: Room Temperature Stable
Form: Liquid
Format: Bottle(s)
Volume: 100 ml
Concentrated: 1 X
Culture Type: Adherent Cell Culture
Product Size: 100 mL
Reagent Type: Trypsin Replacement
Endotoxin Level: Low
Green Features: Energy efficient, Sustainable packaging, Less hazardous
Shipping Condition: Room Temperature

内容及储存

Store at room temperature

life CTS TrypLE Select Enzyme

描述

TrypLE? Select CTS?, a non-animal alternative for porcine Trypsin, is a recombinant enzyme derived from microbial fermentation. The GIBCO? CTS? product line enables you to reduce your burden in qualifying reagents during your transition from research applications to clinical applications.

?GIBCO? TrypLE? Select ? CTS? enables you to reduce your burden in qualifying reagents during your transition from research to clinical applications.
?Non-animal or human origin
?Reduced risk of viral or prion contamination
?Removal kinetics and recovery viability comparable to porcine trypsin
?Demonstration of activity on multiple cell lines, including strongly attached lines
?Stable at room temperature (15 to 30°C) for up to 6 months.
?Manufactured using dedicated, autoclaved or disposable equipment.

TrypLE? Select CTS?, a non-animal alternative for porcine Trypsin, is a recombinant enzyme derived from microbial fermentation. This material is used for the dissociation of attachment-dependent cell lines from plasticware. TrypLE Select has demonstrated the ability to dissociate cells cultured both in serum-free and serum supplemented systems. The product is provided as a 1X stock solution, formulated in DPBS with 1mM EDTA. The concentration of rProtease is proprietary to
Invitrogen Corp.

GIBCO? CTS? products are of high quality, and are supplied with harmonized documentation such as Certificates of Analysis, Certificates of Origin, and access to authorization letters for our Drug Master Files, as appropriate. The GIBCO? CTS? product line enables you to reduce your burden in qualifying reagents during your transition from research applications to clinical applications.

For Research Use or Manufacturing of Cell, Gene, or Tissue-Based Products. CAUTION: Not intended for direct administration into humans or animals

 

life RNA保护液RNAlater Stabilization SolutionAM7021

life RNA保护液RNAlater Stabilization Solution

  • 产品型号:  AM7021
  • 简单描述
  • life RNA保护液RNAlater Stabilization Solution规格:500ml保存温度:室温保存
详细介绍

life RNA保护液RNAlater Stabilization Solution

描述

Ambion? RNAlater? 组织收集: RNA稳定液是一种无毒的水溶性组织保存试剂,能够快速渗入组织内部,稳定并保护细胞RNA。提供一瓶500毫升装。

?简化的试剂即可迅速灭活RNA酶并稳定组织或细胞内RNA

? 无需液氮冷冻样本或迅速将样本送回实验室冰箱中

? 大多数组织样本无需冷冻或研磨

? 实验室外组织收集的*选择

? 在37°C、25°C和4°C下 RNA 分别可保持稳定1天、1周和1个月,-20°C下可长期稳定保存

? 与多种RNA分离方法相兼容,包括大部分Ambion的RNA分离试剂盒

RNAlater? 稳定并保护未损伤未冷冻样本中的细胞RNA,无需迅速处理组织样本和对样本进行液氮冷冻用于延迟处理。获取组织块并将其浸泡于
RNAlater? 中保存,它不影响后续RNA分离所得RNA的质量和数量。RNAlater? 可添加给细胞团甚至培养基中的细胞。然后可对样本进行冷冻或非冷冻保存。

life RNA保护液RNAlater Stabilization Solution

RNAlater? 的使用

切好的组织块(各维尺寸均小于0.5cm)只需简单地在室温下浸入约5体积的 RNAlater? (例如,0.5g的样本需要约2.5ml RNAlater? )中。溶液渗入细胞,稳定RNA。然后样本可以在-20°C长期保存(组织不会冻结)、4°C保存达1个月或25°C保存达1周。如需分离RNA,您只需将组织从 RNAlater? 中取出,当作刚获得的样本进行处理即可。大多数组织可直接转入裂解缓冲液

储存:Store at room temperature. All reagents are guaranteed stable for 6 months when properly stored.