日本同仁化学Hampton蛋白结晶试剂盒Iron(III) chloride hexahydrate/HR2-717- DOJINDO

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Products > Optimize Reagents > Optimize – Salts > Iron(III) chloride hexahydrate

Iron(III) chloride hexahydrate

Hampton蛋白结晶试剂盒Iron(III) chloride hexahydrate/HR2-717

CAT NO

HR2-717

NAME

1.0 M Iron(III) chloride hexahydrate

DESCRIPTION

100 mL

PRICE

$79.00

支持材料

Hampton蛋白结晶试剂盒Iron(III) chloride hexahydrate/HR2-717 HR2-717 Iron(III) chloride hexahydrate SDS

应用

  • Crystallization grade Iron(III) chloride hexahydrate for formulating screens or for optimization
  • 结晶级氯化铁 (III) 六水合物,用于筛选或优化

特征

  • Sterile filtered solution
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
  • 无菌过滤溶液
    在 1+ 型超纯水中配制:25°C 时电阻率为 18.2 兆欧厘米,总有机碳 < 5 ppb,无细菌(<1 细菌 (CFU/ml)),无热原(<0.03 内毒素 (EU/ml)) , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述Iron(III) chloride hexahydrate

Synonyms: Ferric chloride hexahydrate
FeCl3 • 6H20
Mr 270.29
CAS Number [10025-77-1]
EC Number 231-729-4
RTECS NO5425000
Merck 14,4019
MDL Number MFCD00149712
PubChem Substance ID 24868480
UN Number 3260 8/PG 3
Purity 98 – 102%

Measured pH range: 0.6 – 1.0 at 25°C
Measured Conductivity Range: 90.0 – 97.8 mS/cm at 25°C

别名:六水氯化铁
FeCl3 • 6H20
270.29 先生
CAS 编号 [10025-77-1]
欧盟编号 231-729-4
RTECS NO5425000
默克 14,4019
MDL 编号 MFCD00149712
PubChem 物质 ID 24868480
联合国编号 3260 8/PG 3
纯度 98 – 102%

测量的 pH 值范围:25°C 时 0.6 – 1.0
测量的电导率范围:25°C 时 90.0 – 97.8 mS/cm

As ≤0.0005%
Ca ≤0.0005%
Cd ≤0.001%
Cl ≤0.002%
Co ≤0.005%
Cr ≤0.01%
Cu ≤0.002%
Fe2+ ≤0.002%
K   ≤0.005%
Mg ≤0.001%
Mn ≤0.1%
N   ≤0.001%
Na ≤0.01%
Ni  ≤0.005%
NO3 ≤0.01%
Pb  ≤0.002%
PO4 ≤0.01%
SO4 ≤0.005%
Zn  ≤0.002%

Iron(III) chloride hexahydrate

日本同仁化学Iron Assay Kit -Colorimetric-试剂盒货号:I291 Iron Assay Kit -Colorimetric-组织铁离子定量试剂盒- DOJINDO

上海金畔生物科技有限公司代理日本同仁化学 DOJINDO代理商全线产品,欢迎访问官网了解更多信息

Iron Assay Kit -Colorimetric-试剂盒货号:I291
Iron Assay Kit -Colorimetric-组织铁离子定量试剂盒
Iron Assay Kit -Colorimetric-
商品信息
储存条件:0-5度保存
运输条件:室温

特点:

● 适合组织检测

● 配有标准品,可定量二价、三价铁含量

● 吸光度法

下载说明书
产品文献

选择规格:
50tests

现货

 
组织/细胞检测

试剂盒配套计算器(点击查看下载)

Iron Assay Kit -Colorimetric-试剂盒货号:I291 Iron Assay Kit -Colorimetric-组织铁离子定量试剂盒

Iron Assay Kit -Colorimetric-试剂盒货号:I291 Iron Assay Kit -Colorimetric-组织铁离子定量试剂盒

活动进行中
试剂盒内含
产品概述
原理
金属离子选择性
加标回收实验
实验例
参考文献

活动进行中

订购满5000元,200元礼品等你拿

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NO.3.    Cell Counting Kit-8    细胞增殖毒性检测

NO.4.    ROS Assay Kit -Highly Sensitive DCFH-DA-    ROS检测

NO.5.    GSSG/GSH Quantification Kit II    氧化型/还原型谷胱甘肽

 

试剂盒内含

Iron Assay Kit -Colorimetric-试剂盒货号:I291 Iron Assay Kit -Colorimetric-组织铁离子定量试剂盒

产品概述

铁是生物体内最重要的金属元素之一,生物体内铁元素的含量虽少,但有着重要的生理作用。近年来,随着铁死亡概念的提出,细胞内铁离子的代谢过程的研究逐渐成为了研究热点。同仁化学研究所开发的 Iron Assay Kit-Colorimetric-试剂盒 是一套操作简便的比色法铁离子检测试剂盒。与其他市面上的检测试剂盒 相比 ,可以在更短的时间内进行快速检测。通过比较组织裂解液中的铁离子探针 3-(2-Pyridyl)-5,6-bis(5-sulfo-2-furyl)-1,2,4-triazine, disodium salt的 吸光度与还原成二价铁离子的三价铁标准品的吸光度,即可确定组织样品中的二价铁含量。同时,还可以通过试剂盒中的还原剂将样品中的三价铁还原成二价铁,以确定总铁含量,进而计算出样品中三价铁的含量。

原理

Iron Assay Kit -Colorimetric-试剂盒货号:I291 Iron Assay Kit -Colorimetric-组织铁离子定量试剂盒

金属离子选择性

Iron Assay Kit -Colorimetric-试剂盒货号:I291 Iron Assay Kit -Colorimetric-组织铁离子定量试剂盒

加标回收实验

Iron Assay Kit -Colorimetric-试剂盒货号:I291 Iron Assay Kit -Colorimetric-组织铁离子定量试剂盒

实验例

组织样品中的Fe2+和 Fe3+的检测

1. 称量 100 mg肝脏组织 。

2. 加入 1 ml冷 PBS,移液器吹打 20次, Vortex振荡 10 s 14,000 RPM离心 4 min,去除上清 。

3. 将样品转移至研钵,加入液氮充分研磨成粉末后,加入 1.3 ml Assay Buffer,回收至微管中。

4. 将含有样品的微管置于超声波水浴中 5 min。(为防止样品温度上升,每 1 min间隔置于冰浴上 20 s)。

5. 16,000 g离心 10 min,除去不溶物。

6. 取 1300 μl上清液,每管分别加入 400 μl上清液,制成二价铁样品、总铁样品、样品空白。

7. Standard管 中加入 Reducer solution。 (参考图 2 H管 30 μl、 G~C管 20 μl、 B管 40 μl、 A管 20 μl)。

二价品样品管中加入 20 μl Assay Buffer。

总铁样品管中加入 20 μl Reducer solution。

样品空白管中加入 20 μl Assay Buffer。

将所有的 Standard管、样品管、样品空白管 37 培养 15 min。

8. 参考表 1和表 2,加入至 96孔板中 37 培养 60 min,检测 593 nm处的吸光度。

9. 将酶标仪数据拷贝至 “Iron Assay Kit – Excel表 ”,自动计算 出二价铁和三价铁浓度。

Iron Assay Kit -Colorimetric-试剂盒货号:I291 Iron Assay Kit -Colorimetric-组织铁离子定量试剂盒

Iron Assay Kit -Colorimetric-试剂盒货号:I291 Iron Assay Kit -Colorimetric-组织铁离子定量试剂盒

参考文献

No.       检测对象                                           引用 (含链接) 影响因子
1 组织

(小鼠肿瘤组织)

H.   Ren, J. Yong, Q. Yang, Z. Liu, Y. Xu, H. Wang, X. Jiang, W. Miao, X. Li, “Self-assembled FeS-based cascade   bioreactor with enhanced tumor penetration and synergistic treatments to trigger   robust cancer immunotherapy”, Acta Pharm. Sin. B2021,   doi:10.1016/j.apsb.2021.05.005. 7.097
2 细胞

Kasumi-1

(人急性白血病细胞)

L.   Zhang, Y. Song, K. Cao, Y. Du, M. Han, Z. Shi, F. Yan and S. Feng, “Hepcidin-Based   Nanocomposites for Enhanced Cancer Immunotherapy by Modulating Iron   Export-Mediated N6-Methyladenosine RNA Transcript”, Adv. Funct.   Mater.2021, doi: 10.1002/adfm.202107195. 18.808
3 细胞

(II型肺泡细胞)

H.   Cheng, D. Feng, X. Li, L. Gao, S. Tang, W. Liu, X. Wu, S. Yue, C. Li, Z. Luo,   “Iron   deposition-induced ferroptosis in alveolar type II cells promotes the   development of pulmonary fibrosis”, Biochim. Biophys. Acta,   Mol. Basis Dis.2021, doi: 10.1016/j.bbadis.2021.166204. 5.187
4 细胞

MV4-11; Kasumi-1; NB4

(白血病细胞)

Y.   Du, M. Han, K. Cao, Q. Li, J. Pang, L. Dou, S. Liu, Z. Shi, F. Yan and S.   Feng, “Gold   Nanorods Exhibit Intrinsic Therapeutic Activity via Controlling N6‑Methyladenosine   Based Epitranscriptomics in Acute Myeloid   Leukemia”, ACS Nano2021, doi:   10.1021/acsnano.1c05547. 15.881
5 组织

(人软骨组织)

H.   Ren, J. Yong, Q. Yang, Z. Yang, Z. Liu, Y. Xu, H. Wang, X. Jiang, W. Miao, X.   Li, “Contribution   of ferroptosis and GPX4’s dual functions to osteoarthritis progression”,   EBioMedicine2022, doi: 10.1016/j.ebiom.2022.103847. 8.143
6 组织

(小鼠肺、肝脏、肾脏)

T.   Wu, X. Wang, M. Chen, X. Zhang, J. Zhang, J. Cheng, L. Kong, M. Tang,”Respiratory exposure to graphene   quantum dots causes fibrotic effects on lung, liver and kidney of mice”,   Food Chem. Toxicol.2022, doi: 10.1016/j.fct.2022.112971. 6.023
7 组织

(小鼠海马组织)

T.   Wu, X. Wang, J. Cheng, X. Liang, Y. Li, M. Chen, L. Kong and M. Tang, “Nitrogen‑doped   graphene quantum dots induce ferroptosis through disrupting calcium   homeostasis in microglia”, Part. Fibre Toxicol.2022,   doi: 10.1186/s12989-022-00464-z. 9.4
8 组织

(宫颈管粘膜)

T.   Wang, M. Gong, Y. Cao, C. Zhao, Y. Lu, Y. Zhou, S. Yao, J. Chen, C. Zhao and   R. Ju, “Persistent   ferroptosis promotes cervical squamous intraepithelial lesion development and   oncogenesis by regulating KRAS expression in patients with high risk-HPV   infection”, 2022Cell Death Discovery, doi:   10.1038/s41420-022-01013-5. 5.241
9 组织

(小鼠肺组织)

M.   Li, L. Fu, B. Lv, Y. Xiang, H. Xiang, D. Xu, H. Zhao, “Arsenic   induces ferroptosis and acute lung injury through mtROS-mediated   mitochondria-associated endoplasmic reticulum membrane dysfunction”,   Ecotoxicol. Environ. Saf.2022, doi: 10.1016/j.ecoenv.2022.113595. 6.291
10 细胞

BEL-7402, SMMC-7721

(人肝癌细胞系)

L.   Guan, F. Wang, M. Wang, S. Han, Z. Cui, S. Xi, H. Xu and S. Li, “Downregulation of HULC   Induces Ferroptosis in Hepatocellular Carcinoma via Targeting of the   miR-3200-5p/ATF4 Axis”, 2022Oxid. Med. Cell.   Longevity, doi: 10.1155/2022/9613095. 6.543
11 组织

(小鼠肺部组织)

Z.   Zeng, H. Huang, J. Zhang, Y. Liu, W. Zhong, W. Chen, Y. Lu, Y. Qiao, H. Zhao,   X. Meng, F. Zou, S. Cai, H. Dong, “HDM induce airway   epithelial cell ferroptosis and promote inflammation by activating ferritinophagy in asthma”,   FASEB J.2022, doi: 10.1096/fj.202101977RR. 5.191
12 组织

(大豆叶片)

Y.   Cheng, H. Zhang, W. Zhu, Q. Li, R. Meng, K. Yang, Z. Guo, Y. Zhai, R. Ji, H.   Peng, D. Dou, M. Jing, “Ferroptosis   induced by the biocontrol agent Pythium oligandrum enhances   soybean resistance to Phytophthora sojae”,   Environ. Microbiol., doi: 10.1111/1462-2920.16248. 5.476
13 组织

(小鼠睾丸组织)

Y.   Zhao, H. Zhang, J. Cui, J. Wang, M. Chen, H. Wang, X. Li, J. Li, “Ferroptosis is   critical for phthalates driving the blood-testis barrier dysfunction via   targeting transferrin receptor”, Redox Biol.2022,   doi: 10.1016/j.redox.2022.102584. 10.787
14 组织

(小鼠结肠组织)

F.   Yang, Y. Chen, Y. Xiao, H. Jiang, Z. Jiang, M. Yang, M. Li, Y. Su, Z. Yan, Y.   Lin, D. Li, “pH-sensitive   Molybdenum (Mo)-based polyoxometalate nanoclusters have therapeutic efficacy   in Inflammatory Bowel disease by counteracting ferroptosis”, Pharmacol.   Res.2023, doi: 10.1016/j.phrs.2023.106645. 10.334
15 组织

(小鼠肺部组织)

W.   Tang, J. Qin, Y. Zhou, W. Wang, F. Teng, J. Liu, L. Yi, J. Cui, X. Zhu, S.   Wang, J. Dong, Y. Wei, “Regulation of   ferroptosis and ACSL4-15LO1 pathway contributed to the anti-asthma effect of   acupuncture”, Int. Immunopharmacol.2023, doi:   10.1016/j.intimp.2022.109670. 5.714
16 组织

(小鼠心脏组织)

H.   Hu, L. Li, H. Zhang, Y. Zhang, Q. Liu, M. Chen, J. Ning, Y. Pang, W. Hu, Y. Niu,   R. Zhang, “Mechanism   of YY1 mediating autophagy dependent ferroptosis in PM2.5 induced cardiac fibrosis”, Chemosphere2023,   doi: 10.1016/j.chemosphere.2023.137749. 8.943
17 细胞

(HK-2, 人肾皮质近曲小管上皮细胞)

L.   Kang, D. Wang, T. Shen, X. Liu, B. Dai, D. Zhou, H. Shen, J. Gong, G. Li, Y.   Hu, P. Wang, X. Mi, Y. Zhang, X. Tan, “PDIA4 confers   resistance to ferroptosis via induction of ATF4/SLC7A11 in renal cell   carcinoma”, Cell Death Dis.2023, doi:   10.1038/s41419-023-05719-x. 9.685
18 细胞

(HK-2, 人肾皮质近曲小管上皮细胞)

C.   Dong, C. Song, Z. He, Q. Song, T. Song, J. Liu, Y. Xiong, X. Su, J. Zhou, X.   Yang, W. Liao, “Protective   efficacy of Schizandrin B on ameliorating   nephrolithiasis via regulating GSK3β/Nrf2 signaling-mediated ferroptosis in vivo   and in vitro”, 2023Int. Immunopharmacol.,   doi:10.1016/j.intimp.2023.110042. 5.714
19 组织

(小鼠肺部组织)

B.   Guo, Z. Zuo, X. Di, Y. Huang, G. Gong, B. Xu, L. Wang, X. Zhang, Z. Liang, Y.   Hou, X. Liu, Z. Hu, “Salidroside   attenuates HALI via IL-17A-mediated ferroptosis of alveolar   epithelial cells by regulating Act1-TRAF6-p38 MAPK pathway”, 2022,   Cell Commun. Signaling, doi: 10.1186/s12964-022-00994-1. 7.525
20 组织

(小鼠睾丸组织)

J.   Ding, B. Lu, L. Liu, Z. Zhong, N. Wang, B. Li, W. Sheng, Q. He, “Guilu-Erxian-Glue   alleviates Tripterygium wilfordii polyglycoside-induced oligoasthenospermia in rats by   resisting ferroptosis via the Keap1/Nrf2/GPX4 signaling pathway”, Pharm.   Biol.2023, doi:10.1080/13880209.2023.2165114. 3.889
21 细胞

(人髓核细胞)

X.   Yang, Y. Chen, J. Guo, J. Li, P. Zhang, H. Yang, K. Rong, T. Zhou, J. Fu, J.   Zhao, “Polydopamine   Nanoparticles Targeting Ferroptosis Mitigate Intervertebral Disc Degeneration   Via Reactive Oxygen Species Depletion, Iron Ions Chelation, and GPX4   Ubiquitination Suppression”, Adv. Sci., 2023, doi:10.1002/advs.202207216. 17.521
22 组织

(小鼠心脏组织)

J.   Pan, W Xiong, A. Zhang, H. Zhang, H. Lin, L. Gao, J. Ke, S. Huang, J. ZHang,   J. Gu, A. Chang, C. Wang, “The   Imbalance of p53–Park7 Signaling Axis Induces Iron Homeostasis Dysfunction in   Doxorubicin-Challenged Cardiomyocytes”, Adv. Sci, 2023,   doi:10.1002/advs.202206007. 17.521
23 细胞

(H9c2, 4T1)

T.   Chen, Y. Qin, Y. Li, Y. Li, J. Luo, L. Fan, M. Feng, Z. Wang and Y. Zhao, “Chiral Polymer Micelles   Alleviate Adriamycin Cardiotoxicity via Iron Chelation and Ferroptosis   Inhibition”, Adv. Funct. Mater., 2023, doi:10.1002/adfm.202300689. 19.923
24 细胞

(乳腺癌细胞)

Z.   ZHu, H. Shen, J. Xu, Z. Fang, G. Wo, Y. Ma, K. Yang, Y. Wang, Q. Yu, J.   Tang., “GATA3   mediates doxorubicin resistance by inhibiting CYB5R2-catalyzed iron reduction   in breast cancer cells”, Drug Resistance Updates, 2023,   doi:10.1016/j.drup.2023.100974. 22.841
25 组织

(the brains of 3 × Tg-AD mice)

Xinlu Li, Jianfeng Chen, Wennuo Feng ,Chao Wang, Minyu Chen, Yifan Li, Jinghong Chen, Xinwei Liu, Qiong Liu, Jing Tian,“Berberine ameliorates iron levels and ferroptosis in the brain of 3 × Tg-AD mice”2023, PHYTOMEDICINE, doi:10.1016/j.phymed.2023.154962

 

7.9
26 组织

(Testicular tissues)

Lipeng Li , Zijie Pei , Ruiting Wu , Yaling Zhang , Yaxian Pang , Huaifang Hu , Wentao Hu , Zihan Geng , Tengfei Feng , Yujie Niu , Guimin Hao , Rong Zhang“FDX1 regulates leydig cell ferroptosis mediates PM2.5-induced testicular dysfunction of mice”,Ecotoxicology and Environmental Safety,2023,doi.org/10.1016/j.ecoenv.2023.115309 6.8
27 细胞(macrophage) Wenlin Yuan , Yuting Yang , Yingming Wei , Xufei Yu , Jiaqi Bao , Jiahui Zhong , Zhongxiu Wang , Lili Chen“Macrophage ferroptosis promotes MMP2/9 overexpression induced by hemin in hemorrhagic plaque”,Thromb Haemost2023,doi.org/10.1016/j.intimp.2023.110916 6.7
28 细胞(HT-22) Zixuan Yuan,ORCID,Xiaoming Zhou ,Yan Zou ,Bingtao Zhang ,Yao Jian ,Qi Wu ,Shujuan Chen and Xin Zhang“Hypoxia Aggravates Neuron Ferroptosis in Early Brain Injury Following Subarachnoid Hemorrhage via NCOA4-Meditated Ferritinophagy”,Antioxidants2023,doi.org/10.3390/antiox12122097 7.0