Invitrogen miRNA 分离试剂盒内容与储存• 30 mL miRNA 洗涤液 I(室温)• 50 mL 洗涤液 2/3(室温)• 80 个收集管(室温)• 40 个滤芯(室温)• 100 mL 裂解/结合缓冲液 (4°C)• 10 mL miRNA 匀浆添加剂 (4°C)• 1.4 mL 凝胶上样缓冲液 II (-20°C)
Invitrogen Gateway BP Clonase II酶混合物内容与储存Gateway™ BP Clonase™ II 酶混合物包含蛋白酶 K 溶液 (2 µg/µL) 和阳性对照载体。在 -20°C 或 -80°C 下储存。妥善储存时,可保证稳定储存 6 个月。
详细介绍
Invitrogen Gateway BP Clonase II酶混合物
Gateway ™BP Clonase™ II 酶混合物可催化 PCR 产物体外重组或从克隆(含 attB 位点)和供体载体(含 attP 位点)亚克隆 DNA 片段,生成入门克隆。Gateway™ BP Clonase™ II 是酶和缓冲液的单一混合物,能够以较少的移液步骤方便地构建十微升反应体系。
For Research Use Only. Not for use in diagnostic procedures.
Invitrogen Gateway BP Clonase II酶混合物
Gateway ™BP Clonase™ II 酶混合物可催化 PCR 产物体外重组或从克隆(含 attB 位点)和供体载体(含 attP 位点)亚克隆 DNA 片段,生成入门克隆。Gateway™ BP Clonase™ II 是酶和缓冲液的单一混合物,能够以较少的移液步骤方便地构建十微升反应体系。
规格
运输条件
干冰
酶
BP Clonase
兼容缓冲液
酶缓冲液
数量
20 次反应
产品类型
BP Clonase 酶混合物
产品线
Clonase™,Gateway™
内容与储存
Gateway™ BP Clonase™ II 酶混合物包含蛋白酶 K 溶液 (2 µg/µL) 和阳性对照载体。在 -20°C 或 -80°C 下储存。妥善储存时,可保证稳定储存 6 个月。
invitrogen MPK1096 Neon Transfection System 内容及储存• 3 × 1 ml Resuspension Buffer R• 3 × 1 ml Resuspension Buffer T• 2 × 150 ml Electrolytic Buffer E• 96 × 10 µl Neon® tips• 20 Neon® ele
详细介绍
invitrogen MPK1096 Neon Transfection System 10 µL Kit
描述
The Neon® Transfection System 10 µL Kit is designed specifically for use with the Neon® Transfection System. Use this kit for transfection volumes of 10 µL, containing 5 × 104–2 × 105adherent cells or 1 × 105–5 × 105 suspension cells. Cells that have been transfected using the included 10 µL Neon® Tips are ready to be washed and plated into a multi-well culture dish
invitrogen MPK1096 Neon Transfection System 10 µL Kit
规格
Cell Type:
Established Cell Lines, Hard-to-Transfect Cells, Primary Cells, Stem Cells
单位定义:一个单位的SuperScript? III RT 是在37°C 的反应体系中,使用poly(A)?oligo(dT)12-18 作为模板?引物,在10 分钟内将1 纳摩尔脱氧核糖核苷酸掺入酸沉淀材料所需的酶量(3)。
单位反应条件:50 mM Tris-HCl (pH 8.3)、40 mM KCl、6 mM MgCl 2、1 mM DTT。0.5 更看更多mM [ 3 H]dTTP、0.1 mM poly(A)、0.1 mM oligo(dT)12-18、0.1 mg/ml BSA 和酶,共50 μl,温度37°C,时间10 分钟。
Life Technologies 大家庭中的细胞培养产品。 Life Technologies公司已准备就绪,以满足您的细胞培养需要一个全面的选择,包括培养基,营养添加剂,盐溶液,缓冲液,化学物质,解离试剂和转染的试剂。您可以依靠严格的质量控制和,以保证您的满意。仅供研究使用。 不用于任何动物或人类治疗或诊断使用。
INVITROGEN反转录酶II,SuperScript II Reverse Transcript
产品型号: 18064-014
简单描述
INVITROGEN反转录酶II,SuperScript II Reverse Transcriptase货号18061014规格:10,000 units现货供应
详细介绍
INVITROGEN反转录酶II,SuperScript II Reverse Transcriptase货号18061014
描述 反转录(RT)superscript®II是一个改进版本superscript®RT。It is a聚合酶,DNA链synthesizes有互补单链RNA从DNA、RNA、DNA杂交。这种酶是转基因工程by the introduction of点突变in the RNase H活性中心to reduce RNase H活性。这eliminates降解RNA分子结构的变化在*链cDNA Synthesis and gives superscript®II RT性能特性上。
INVITROGEN反转录酶II,SuperScript II Reverse Transcriptase货号18061014
描述
SuperScript® II Reverse Transcriptase (RT) is an improved version of SuperScript® RT. It is a DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid. This enzyme is genetically engineered by the introduction of point mutations in the RNase H active center to reduce RNase H activity. This structural modification eliminates degradation of RNA molecules during first-strand cDNA synthesis and gives SuperScript® II RT superior performance characteristics.
• Yield – Reduced RNase H activity results in greater first-strand cDNA yields • Size of cDNA – Generation of RT-PCR products up to 12 kb • Thermostability – Full activity at 42°C • Flexibility – Uses either total or poly(A)+ RNA
Performance and Quality Testing: SDS-PAGE purity; endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease assays; and yield and length of cDNA product.
Unit Definition: One unit of SuperScript® II is the amount of enzyme required to incorporate 1 nmole of deoxyribonucleotide into acid-precipitable material in 10 min. at 37°C using poly(A)•oligo(dT)12-18 as template•primer (3).
Unit Reaction Conditions: 50 mM Tris-HCl (pH 8.3), 40 mM KCl, 6 mM MgCl2, 1 mM DTT, 0.5 mM [3H]dTTP, 0.1 mM poly(A), 0.1 mM oligo(dT)12-18, 0.1 mg/ml BSA, and enzyme in 50 µl for 10 min. at 37°C.
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
Invitrogen LR酶,Gateway LR Clonase II Enzyme mix描述Gateway® LR Clonase® II 酶混合物催化入门载体(含有侧翼是 attL 位点的目的基因)与目的载体(含有 attR 位点)进行体外重组,产生表达克隆。 Gateway® LR Clonase® II 为包含酶和缓冲剂的单一混合物,所需的移液步骤更少,因此便于设置十微升反应体
详细介绍
Invitrogen LR酶,Gateway LR Clonase II Enzyme mix
规格:20 reactions
现货供应
Invitrogen LR酶,Gateway LR Clonase II Enzyme mix
Gateway® LR Clonase® II 酶混合物催化入门载体(含有侧翼是 attL 位点的目的基因)与目的载体(含有 attR 位点)进行体外重组,产生表达克隆。 Gateway® LR Clonase® II 为包含酶和缓冲剂的单一混合物,所需的移液步骤更少,因此便于设置十微升反应体
规格
Enzyme:
LR Clonase
Product Size:
20 reactions
Shipping Condition:
Dry Ice
内容及储存
Gateway® LR Clonase® II enzyme mix includes proteinase K solution (2 µg/µl) and a positive control vector. Store at -20°C or -80°C. Guaranteed stable for 6 months when properly stored.
Invitrogen TRIzol LS Reagent 100ml货号10296-010规格:100mlTRIzol® LS Reagent 特点:• 设计用于液体样品,例如血清和病毒制品• 使用 TRIzol® LS 试剂与液体样品的比值为 3:1• 有助于多种大分子或小分子 RNA 的分离
详细介绍
Invitrogen TRIzol LS Reagent 100ml货号10296-010
TRIzol® LS Reagent 是苯酚和异硫氰酸胍的即用型单相溶液,设计用于从人、动物、植物、酵母、细菌和病毒来源的液体样品中分离总 RNA。 在样品匀浆或裂解过程中,TRIzol® LS Reagent 一方面保持 RNA 的完整性,另一方面破坏细胞并裂解细胞组分。
TRIzol® LS 试剂分离的总 RNA 不含蛋白质和 DNA 杂质。 可用于 Northern blot 分析,点印迹杂交,多聚 (A)+ 选择,体外翻译,RNA 酶保护分析,RT-PCR 和分子克隆。 对于 RT-PCR,当一个外显子中存在两个引物时, 建议用扩增级 DNase I(商品目录号 18068-015)处理分离的 RNA
描述
TRIzol® LS Reagent is a complete, ready-to-use reagent optimized for the isolation of high-quality total RNA, or the simultaneous isolation of RNA, DNA, and protein from a variety of liquid samples. This monophasic solution of phenol and guanidine isothiocyanate is designed to isolate separate fractions of RNA, DNA, and proteins from liquid samples of human, animal, plant, yeast, bacterial, and viral origin, typically within one hour.
• Formulated for use with liquid samples such as serum and virus preparations • Facilitates recovery of RNA, DNA, and protein from a single liquid sample • Offers excellent lysis capability, even with difficult biological fluids