Peprotech Recombinant Human HGF Synonyms:Scatter Factor (SF), Hepatopoietin (HPTA)
详细介绍
Recombinant Human HGF
Catalog Number:
100-39
Synonyms:
Scatter Factor （SF）, Hepatopoietin （HPTA）
Description:
HGF is a mesenchymally derived potent mitogen for mature parenchymal hepatocyte cells and acts as a growth factor for a broad spectrum of tissues and cell types. HGF signals through a transmembrane tyrosine kinase receptor known as MET. Activities of HGF include induction of cell proliferation, motility, morphogenesis, inhibition of cell growth, and enhancement of neuron survival. HGF is a crucial mitogen for liver regeneration processes, especially after partial hepatectomy and other liver injuries. Human and murine HGF are cross-reactive. Human HGF is expressed as a linear 697 amino acid polypeptide precursor glycoprotein. Proteolytic processing of this precursor generates the biologically active form of HGF, which consists of two polypeptide chains （α-chain and β-chain） held by a single disulfide bond resulting in formation of a biologically active heterodimer. The α-chain consists of 463 amino acid residues and four kringle domains. The β-chain consists of 234 amino acid residues.*Manufactured using （BTI-Tn-5B1-4） cells under license from Boyce Thompson Institute for Plant Research, Inc.
Recombinant Human FGF-4 Synonyms:Fibroblast Growth Factor-4, HST-1, transforming protein KS3, HBGF-4
详细介绍
Description:
FGF-4 is a heparin binding growth factor that is a member of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-4 signals through the FGFR 1c, 2c, 3c, and 4. Recombinant human FGF-4 is a 19.7 kDa protein consisting of 182 amino acid residues.
Greater than 95% by SDS-PAGE gel and HPLC analyses.
Biological Activity:
The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF-receptors is ≤ 0.5 ng/ml, corresponding to a specific activity of ≥ 2 x 106units/mg.
Cross Reactivity:
Mouse, Rat, Human, N/A, Pig
Research Interests:
Angiogenesis/ Cardiovascular
FGF Superfamily
Proliferation
Stem Cells & Differentiation
Country of Origin:
USA
Research Use Only. Not for use in diagnostic or therapeutic procedures.
Recombinant Human FGF-4 Synonyms:Fibroblast Growth Factor-4, HST-1, transforming protein KS3, HBGF-4
详细介绍
Description:
FGF-4 is a heparin binding growth factor that is a member of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-4 signals through the FGFR 1c, 2c, 3c, and 4. Recombinant human FGF-4 is a 19.7 kDa protein consisting of 182 amino acid residues.
Greater than 95% by SDS-PAGE gel and HPLC analyses.
Biological Activity:
The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF-receptors is ≤ 0.5 ng/ml, corresponding to a specific activity of ≥ 2 x 106units/mg.
Cross Reactivity:
Mouse, Rat, Human, N/A, Pig
Research Interests:
Angiogenesis/ Cardiovascular
FGF Superfamily
Proliferation
Stem Cells & Differentiation
Country of Origin:
USA
Research Use Only. Not for use in diagnostic or therapeutic procedures.
The three mammalian isoforms of TGF-β, TGF-β1, β2, β3, signal through the same receptor and elicit similar biological responses. They are multifunctional cytokines that regulate cell proliferation, growth, differentiation and motility as well as synthesis and deposition of the extracellular matrix. They are involved in various physiological processes including embryogenesis, tissue remodeling and wound healing. They are secreted predominantly as latent complexes which are stored at the cell surface and in the extracellular matrix. The release of biologically active TGF-β isoform from a latent complex involves proteolytic processing of the complex and /or induction of conformational changes by proteins such as thrombospondin-1. TGF-β1 is the most abundant isoform secreted by almost every cell type. It was originally identified for its ability to induce phenotypic transformation of fibroblasts and recently it has been implicated in the formation of skin tumors. Human TGF-β1 is a 25.0 kDa protein with each subunit containing 112 amino acid residues, linked by a single disulfide bond.
Greater than 98% by SDS-PAGE gel and HPLC analyses.
Biological Activity:
The ED50 was determined by TGF-beta1’s ability to inhibit the mouse IL-4-dependent proliferation of mouse HT-2 cells is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 107 units/mg.
The three mammalian isoforms of TGF-β, TGF-β1, β2, β3, signal through the same receptor and elicit similar biological responses. They are multifunctional cytokines that regulate cell proliferation, growth, differentiation and motility as well as synthesis and deposition of the extracellular matrix. They are involved in various physiological processes including embryogenesis, tissue remodeling and wound healing. They are secreted predominantly as latent complexes which are stored at the cell surface and in the extracellular matrix. The release of biologically active TGF-β isoform from a latent complex involves proteolytic processing of the complex and /or induction of conformational changes by proteins such as thrombospondin-1. TGF-β1 is the most abundant isoform secreted by almost every cell type. It was originally identified for its ability to induce phenotypic transformation of fibroblasts and recently it has been implicated in the formation of skin tumors. Human TGF-β1 is a 25.0 kDa protein with each subunit containing 112 amino acid residues, linked by a single disulfide bond.
Greater than 98% by SDS-PAGE gel and HPLC analyses.
Biological Activity:
The ED50 was determined by TGF-beta1’s ability to inhibit the mouse IL-4-dependent proliferation of mouse HT-2 cells is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 107 units/mg.
Activity Measured by its ability to induce IL6 secretion by NHDF human normal dermal fibroblasts. The ED50 for this effect is typically 26 ng/mL. Measured by its ability to induce IL6 secretion by NIH-3T3 mouse embryonic fibroblast cells. Yao, Z. et al. （1995） Immunity 3:811. The ED50 for this effect is typically 1.57.5 ng/mL. Endotoxin Level <1.0 EU per 1 μg of the protein by the LAL method. Purity >97%, by SDSPAGE under reducing conditions and visualized by silver stain. Formulation Lyophilized from a 0.2 μm filtered solution in HCl. See Certificate of Analysis for details. PREPARATION AND STORAGE Reconstitution Reconstitute at 100 μg/mL in 4 mM HCI. Shipping The product is shipped with polar packs. Upon receipt, store it immediay at the temperature recommended below. Stability & Storage Use a manual defrost freezer and avoid repeated freezethaw cycles. l 12 months from date of receipt, 20 to 70 °C as supplied. l 1 month, 2 to 8 °C under sterile conditions after reconstitution. l 3 months, 20 to 70 °C under sterile conditions after reconstitution. BACKGROUND Interleukin 17 （also known as CTLA8） is a T cellexpressed pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of herpesvirus Saimiri. cDNA clones encoding IL17 have been isolated from activated rat, mouse and human T cells. Human IL17 cDNA encodes a 155 amino acid （aa） residue precursor protein with a 19 amino acid residue signal peptide that is cleaved to yield the 136 aa residue mature IL17 containing one potential Nlinked glycosylation site. Both recombinant and natural IL17 have been shown to exist as disulfidelinked homodimers. At the amino acid level, human IL17 shows 72% and 63% sequence identity with herpesvirus and rat IL17, respectively. An IL17 specific mouse cell surface receptor （IL17 R） has been cloned. While the expression of IL17 mRNA is restricted to activated T cells, the expression of mIL17 R mRNA has been detected in virtually all cells and tissues tested. IL17 exhibits multiple biological activities on a variety of cells including the induction of IL6 and IL8 production in fibroblasts, the enhancement of surface expression of ICAM1 in fibroblasts, activation of NFκB and costimulation of T cell proliferation. The nonglycosylated, E. coliexpressed rhIL17 has been shown to be active on human as well as mouse cell lines. References: 1. Yao, Z. et al. （1995） J. Immunol. 155:5483. 2. Yao, Z. et al. （1995） Immunity 3:811. 3. Rouvier, E. et al. （1993） J. Immunol. 150:5445
SOURCE: This antibody was purified from rabbit serum using protein A agarose. The rabbit was immunized with the recombinant human LC3 [MAP1LC3B （1-120 aa）]. FORMULATION: 100 ?L volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained. STORAGE: This antibody solution is stable for one year from the date of purchase when stored at -20oC. REACTIVITY: This antibody reacts with LC3 （MAP1LC3A, B, C） on Western blotting, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry and Flow cytomrtry. It does not react with GABARAP and GATE-16. MBL抗体代理Human LC3 Polyclonal Antibody MBL公司
APPLICATIONS: Western blotting; 1:1,000 for chemiluminescence detection system Immunoprecipitation; 2 ?L/300 ?L of cell extract from 1 x 107 cells Immunohistochemistry; 1:1,000-1:2,000 Heat treatment is necessary for paraffin embedded sections. Microwave oven; 2 times for 10 minutes each in 10 mM citrate buffer （pH 6.3） Immunocytochemistry; 1:200-1:500 Flow cytometry; 1:200 （final concentration） Detailed procedure is provided in the following PROTOCOLS.
RD Recombinant human IL-17A人白介17因子Source E. coliderived此产品还有小鼠IL-17A
详细介绍
RD Recombinant human IL-17A人白介17因子
DESCRIPTION Source E. coliderived Thr22Ala158 Accession # Q62386.1 Nterminal Sequence Analysis Thr22 Structure / Form Disulfidelinked homodimer Predicted Molecular Mass 15.5 kDa （monomer） SPECIFICATIONS Activity Measured by its ability to induce IL6 secretion by NIH-3T3 mouse embryonic fibroblast cells. Yao, Z. et al. （1995） Immunity 3:811. The ED50 for this effect is typically 0.251.25 ng/mL. Endotoxin Level <1.0 EU per 1 μg of the protein by the LAL method. Purity >97%, by SDSPAGE under reducing conditions and visualized by silver stain. Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein. See Certificate of Analysis for details. PREPARATION AND STORAGE Reconstitution Reconstitute at 25 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin. Shipping The product is shipped at ambient temperature. Upon receipt, store it immediay at the temperature recommended below. Stability & Storage Use a manual defrost freezer and avoid repeated freezethaw cycles. l 12 months from date of receipt, 20 to 70 °C as supplied. l 1 month, 2 to 8 °C under sterile conditions after reconstitution. l 3 months, 20 to 70 °C under sterile conditions after reconstitution. BACKGROUND Interleukin 17 （also known as CTLA8） is a T cellexpressed pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of herpesvirus Saimiri. cDNA clones encoding IL17 have been isolated from activated rat, mouse and human T cells. Mouse IL17 cDNA encodes a 158 amino acid （aa） residue precursor protein with a 21 amino acid residue signal peptide that is cleaved to yield the 137 aa residue mature IL17. Both recombinant and natural IL17 have been shown to exist as disulfide linked homodimers. At the amino acid level, mIL17 shows 57% and 87% sequence identity with herpesvirus and rat IL17, respectively. An IL17 specific mouse cell surface receptor （IL17 R） has been cloned. While the expression of IL17 mRNA is restricted to activated alpha beta TCR+CD4CD8T cells, the expression of mIL17 R mRNA has been detected in virtually all cells and tissues tested. IL17 exhibits multiple biological activities on a variety of cells including: the induction of IL6 and IL8 production in fibroblasts? the enhancement of surface expression of ICAM1 in fibroblasts? activation of NFκB and costimulation of T cell proliferation. References: 1. Kennedy, J. et al., （1996） J. Interferon Cytokine Res. 16:611. 2. Yao, Z. et al., （1995） J. Immunol. 155:5483. 3. Yao, Z. et al., （1995） Immunity 3:811. 4. Rouvier, E. et al., （1993） J. Immunol. 150:5445.
本系列制品是基于Gubler and Hoffman方法(Gene 25:263-269、(1983))， 由各种Poly(A)+RNA制作的质粒DNA型cDNA文库。合成cDNA片段时，使用了含有限制酶Not I位点的Oligo (dT)18 Linker Primer和BamH I (Bgl II)-Sma I 接头，通过这两个限制酶位点，使cDNA片段定向克隆到载体中。克隆前，做了短片段的分离处理，300 bp以下的片段大部分被去除掉。初级文库构建完成后，采用了固体平板培养法，仅对初级文库进行一次扩增，然后使用质粒提取试剂盒，从扩增文库的菌体中提取质粒，调整得到扩增的质粒cDNA文库。使用这种方法得到的扩增文库，尽可能保持了各种cDNA片段的克隆在初级文库中所占的比例。
■ 保存
-20℃。
■ cDNA文库使用的载体和多克隆位点
本cDNA文库制品使用的载体为pAP3neo，含有SV40启动子，可以在哺乳动物细胞中进行表达。同时，该载体含有ssDNA生成的必要f1 ori、也含有便于RNA合成的T7及T3 RNA聚合酶启动子。 GenBank Accession No.AB003468 1. cDNA片段克隆在pAP3neo载体的Bgl II位点和Not I位点之间。 * 由于cDNA片段合成时，使用了BamH I (Bgl II)-Sma I接头，克隆后，载体的Bgl II 位点失效，不能再被Bgl II酶切开。 2. Xba I 位点受dam methylase的影响。
本系列制品是基于Gubler and Hoffman方法 (Gene（1983）25：263-269) 制作的质粒DNA型cDNA文库。合成cDNA片段时，使用了含有限制酶Not I位点的Oligo (dT)18 Linker Primer和BamH I (Bgl II)-Sma I 接头，通过这两个限制酶位点，使cDNA片段定向克隆到载体中。克隆前，做了短片段的分离处理，300 bp以下的片段大部分被去除掉。初级文库构建完成后，采用了固体平板培养法，仅对初级文库进行一次扩增，然后使用质粒提取试剂盒，从扩增文库的菌体中提取质粒，调整得到扩增的质粒cDNA文库。使用这种方法得到的扩增文库，尽可能保持了各种cDNA片段的克隆在初级文库中所占的比例。
■ 保存
-20℃。
■ cDNA文库使用的载体和多克隆位点
本cDNA文库制品使用的载体为pAP3neo，含有SV40启动子，可以在哺乳动物细胞中进行表达。同时，该载体含有ssDNA生成的必要f1 ori、也含有便于RNA合成的T7及T3 RNA聚合酶启动子。 GenBank Accession No.AB003468 1. cDNA片段克隆在pAP3neo载体的Bgl II位点和Not I位点之间。 * 由于cDNA片段合成时，使用了BamH I (Bgl II)-Sma I接头，克隆后，载体的Bgl II 位点失效，不能再被Bgl II酶切开。 2. Xba I 位点受dam methylase的影响。
