BIOTOOL代理 Poly FlAG多肽B23111 B23112

BIOTOOL代理 Poly FlAG多肽

  • 产品型号:  B23111 B23112
  • 简单描述
  • BIOTOOL代理 Poly FlAG多肽The Poly DYKDDDDK (FLAG) Peptide lyophilized powder was synthesized by 23 amino acid residues with molecular weight 2,864 Da. The Asp-Tyr-Lys-Xaa-Xaa-Asp motif is repeated three
详细介绍

BIOTOOL代理 Poly FlAG多肽

Properties

Appearance (Color) White
Appearance (Form) Powder
Form Lyophilized Powder
Purity by HPLC-MS 95.33 % (the latest lot) Poly FLAG Peptide lyophilized powder
Concentration (Recommended working concentration is 100-400 μg/mL for elute FLAG fusion proteins from the Biotool Anti-DYKDDDDK (FLAG) affinity gel.
Shipped in Blue ice
Storage Temperature 2-8°C

Application

For use in competitive elution of DYKDDDDK (FLAG) fusion proteins from the ANTI-FLAGmonoclonal antibody in solution or bound to agarose on the Biotool Anti-DYKDDDDK (FLAG) affinity gel.

Attention

1. Read the User Manual carefully before the first use;
2. Avoid freezing, drying and high-speed centrifugation during use and storage of beads;
3. This product is for R&D use only, not for drug, household, or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.

Procedure

Peptide Elution of DYKDDDDK(FLAG) Fusion Protein from Biotool Anti-DYKDDDDK(FLAG) affinity gel

1. Thoroughly suspend the Anti-Flag Affinity Gel in the vial, for a uniform suspension of the resin. Quickly transfer 10μl of the gel suspension (about 5μl of packed gel volume) to a fresh tube.

2. Add 0.6 mL TBS. Thoroughly suspend the Anti-Flag Affinity Gel by pipetting. Centrifuge the resin at 5000 rpm for 30 secondsand carefully remove the supernatant. Be sure to remove all of the wash buffer without discarding the resin. Repeat 3-4 times.

3. Add 500 μL of cell periplasmic extracts to the washed resin.

4. Gently agitate samples for 2 hours at 4°C.
5. Centrifuge the resin for 30 seconds at 5000 rpm. Transfer the supernatants to a fresh tube.
6. Wash the resin with 0.5mL TBS until the OD280 of the supernatant reads<0.05.
7. Elution of DYKDDDDK (FLAG) Fusion Protein by Competition with Poly DYKDDDDK (FLAG) Peptide. Elute the bound DYKDDDDK(FLAG) Fusion Proteinby competitive elution with five one-column volume aliquots of a solution containing 100-400 ug/mL Poly DYKDDDDK(FLAG) Peptide in TBS. 
8. Recycling the Biotool Anti-DYKDDDDK(FLAG) affinity gel
Poly DYKDDDDK(FLAG) Peptidemay not elute all of the DYKDDDDK(FLAG) Fusion Protein bound to Biotool Anti-DYKDDDDK(FLAG) affinity gel. It is recommended the Biotool Anti-DYKDDDDK(FLAG) Affinity gel be regenerated immediay after use by washing with three 5 mL aliquots of 0.1 M glycine HCl, pH 3.5. The gel should be immediay re-equilibrated in TBS until the effluent is at neutral pH. 
Note: Do not leave the Biotool Anti-DYKDDDDK(FLAG) Affinity gel in glycine HCl for longer than 20 minutes.
9. Storing the Biotool Anti-DYKDDDDK(FLAG) Affinity gel
Wash the Biotool Anti-DYKDDDDK(FLAG) Affinity gel three times with 5 mL of 50% glycerol with 10mM sodium phosphate, 150 mM sodium chloride, pH 7.4, containing 0.02% (w/v) sodium azide. then add another 5 mL of 50% glycerol with 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.4, containing 0.02% (w/v) sodium azideand store at -20°C without draining.

Storage & stability

Store the product at 2-8°C.

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Poly FLAG Peptide lyophilized powder

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Graphene Oxide

 

 

BIOTOOL代理 Poly FlAG多肽

订购信息:

 

B23111 4mg

B23112 25mg

BIOTOOL Anti-DYKDDDDK(Flag)亲和凝胶B23101 B23102

BIOTOOL Anti-DYKDDDDK(Flag)亲和凝胶

  • 产品型号:  B23101 B23102
  • 简单描述
  • BIOTOOL Anti-DYKDDDDK(Flag)亲和凝胶Biotool Anti-Flag(DYKDDDDK) Affinity Gel 由高质量的鼠源 IgG2b 单克隆抗体与sepharose 4B gel 通过供价偶联制备,本产品具有较高的Flag标签融合蛋白加载容量(至少为1.1mg protein /ml gel),杂蛋白非特异结合少,可用于蛋白质免疫共沉淀和少量蛋白质纯化。
详细介绍

BIOTOOL Anti-DYKDDDDK(Flag)亲和凝胶

产品性质

抗体克隆号 1E6
抗体亚型 鼠源IgG2b
抗体纯化方法 Protein A 纯化制备
抗体结合量 7.5 克抗体/升gel
适用范围 蛋白质免疫共沉淀,蛋白质纯化
推荐使用体积 蛋白质免疫共沉淀: 500μl 细胞裂解液 使用 5μl gel
融合蛋白结合容量 至少为1.1mg protein/ml gel
结合特性 甲硫氨酸修饰的N端Flag融合蛋白:Met-FLAG–Protein
N端Flag 融合蛋白:FLAG–Protein
C端 Flag 融合蛋白:Protein-FLAG
运输条件 4°C 冰袋

BIOTOOL Anti-DYKDDDDK(Flag)亲和凝胶

操作步骤:

本推荐方法主要适用于检测可以与 Biotool Anti-Flag Affinity Gel 结合的蛋白。
1、轻微重悬Biotool Anti-Flag Affinity Gel,转移10 μl混合液(5μl gel)至新的离心管中。
2、加入500 μl TBS. 轻轻的重悬Anti-Flag Affinity Gel,10000 rpm 离心30s, 弃上清,重复上述步骤 3-4 次。
3、在上述沉淀中加入500 μl细胞裂解液。
4、4℃ 缓慢孵育2小时。
5、10000 rpm 离心 30s,上清液转移到新的离心管中保存以后检测。
6、上步中得到的沉淀用 0.5 ml TBS 洗 3-4 次,至上清液中 OD280 小于 0.05 为止。
7、上述所得沉淀中加入 10 μl of 2×蛋白上样缓冲液,煮沸 5 min。
8、进行SDS-PAGE 蛋白检测。

BIOTOOL Anti-DYKDDDDK(Flag)亲和凝胶

订购信息:

B23101 1ML

B23102 5ML

B23103 25ML

B23104 100ML

 

DYKDDDDK标签蛋白质免疫沉淀 Buffer Set(FLAG标签)酶试剂盒Takara


DYKDDDDK标签蛋白质免疫沉淀 Buffer Set(FLAG标签)
品牌 Code No. 产品名称 包装量 价格(元) 说明书 数量
Clontech 635687 Immunoprecipitation Buffer Set 30 Rxns ¥6,519 DYKDDDDK标签蛋白质免疫沉淀 Buffer Set(FLAG标签) DYKDDDDK标签蛋白质免疫沉淀 Buffer Set(FLAG标签) DYKDDDDK标签蛋白质免疫沉淀 Buffer Set(FLAG标签)
收藏产品 加入购物车
 
■ 产品描述
DYKDDDDK标签(FLAG标签)是常用的亲水性八肽标签,是鉴定和监控DYKDDDDK(FLAG)标签蛋白质表达的理想选择。将高特异性的anti-DYKDDDDK单克隆抗体(不与内源性的蛋白质产生交叉反应)与琼脂糖珠交联,即可用来从细胞裂解液中纯化或免疫沉淀DYKDDDDK(FLAG)标签蛋白质。本制品是用于DYKDDDDK(FLAG)标签蛋白质免疫沉的buffer set,是高度优化的缓冲液组合,建议与anti-DYKDDDDK琼脂糖珠搭配使用,背景非常低。
 
■ 应用
· Western blotting
· 免疫荧光染色
· ELISA
· 使用琼脂糖珠的蛋白质纯化和免疫沉淀
 
 
 

页面更新:2020-04-07 11:34:14