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fermentas逆转录试剂盒RevertAid First Strand cDNA SynthesThermo K1622

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fermentas逆转录试剂盒RevertAid First Strand cDNA Synthes

  • 产品型号：  Thermo K1622
  • 简单描述
  • fermentas逆转录试剂盒RevertAid First Strand cDNA Synthesis KitK1621 20次K1622 100次
详细介绍

fermentas逆转录试剂盒RevertAid First Strand cDNA Synthesis Kit说明书

Thermo Scientific RevertAid First Strand cDNA Synthesis Kit是一套用于以RNA为模板高效合成*链cDNA的完整系统，可合成长达13kb的cDNA。试剂盒配套提供的RiboLockRNase Inhibitor，可有效防止RNA模板的降解。试剂盒配套提供oligo(dT)18引物和随机六聚体引物。oligo(dT)18引物可以选择性地与mRNA中的poly (A)尾结合。随机六聚体引物无需poly (A)尾，因此可转录mRNA 5’-端区域，也可以无poly (A)尾的RNA (如micoRNA)为模板合成cDNA。该试剂盒也可使用基因特异性引物。

优点：

  • 合成全长的*链cDNA，zui长可合成13kb的cDNA
  • *反应温度为42℃
  • *一提供RT反应所需的所有组分

应用:

  • *链cDNA合成，作为RT-PCR和实时RT-qPCR的模板
  • 构建全长cDNA文库
  • 反义RNA合成

fermentas逆转录试剂盒RevertAid First Strand cDNA Synthesis Kit 

品名 货号 品牌 规格 售价
RevertAid? First Strand cDNA Synthesis Kit K1622 Fermentas（MBI） 100 react 询价

RevertAid First Strand cDNA Synthesis Kit
Features
·         Full-length first strand cDNA up to 13 kb.
·         Increased reaction temperatures in the range of 42-50°C.
·         Supplied with the recombinant RiboLock? RNase Inhibitor.
·         Complete – oligo(dT)18 and random hexamer primers included with the kit.
Applications
·         First strand cDNA synthesis for RT-PCR and real-time RT-PCR (1, 2).
·         Construction of full length cDNA libraries.
·         Generation of probes for hybridization.
·         aRNA synthesis.
Description
The RevertAid? First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates. The kit is suitable for synthesis of cDNA up to 13 kb.
The kit uses RevertAid? Reverse Transcriptase which has lower RNase H activity, compared to AMV reverse transcriptase.
The recombinant RiboLock? RNase Inhibitor, supplied with the kit, effectively protects RNA template from degradation. It is fully compatible with reverse transcription reaction, as it maintains activity at temperatures up to 55°C.
The kit is supplied with both oligo(dT)18 and random hexamer primers. The oligo(dT)18 anneals selectively on the poly(A) tail of mRNA. Random hexamer primers do not require the presence of poly(A). Therefore, they can be used for transcription of the 5-end regions of mRNA or cDNA synthesis using RNA without poly(A) tail e.g. micro RNAs. Gene-specific primers may also be used with the kits.
The first strand of cDNA can be directly used as a template in PCR (see RT-PCR protocol), real-time PCR or in second strand cDNA synthesis (see protocol). 
Quality Control
The kit is functionally tested in RT-PCR using 100 fg control GAPDH RNA and GAPDH control primers generated a 496 bp product visible on agarose gel after ethidium bromide staining.

is Kit

fermentas逆转录试剂盒RevertAid First Strand cDNA SynthesThermo K1622
RevertAid First Strand cDNA Synthesis Kit合成全长cDNA
1 μg小鼠心脏总RNA作为模板，用oligo(dT)18 引物进行逆转录反应。由此合成的cDNA，再作为Taq DNA Polymerase后续PCR扩增的模板。末端2024 bp片段的成功扩增，表明长度为13.8 kb的全长RNA片段得到成功的逆转录。

产品组成
RevertAid First Strand cDNA Synthesis Kit包含RevertAid Reverse Transcriptase、RiboLock RNase Inhibitor、5x Reaction Buffer、dNTP Mix、Oligo(dT)18 引物、随机六聚体引物、对照GAPDH RNA、10 μM 正向GAPDH引物、10 μM反向GAPDH 引物以及nuclease-free water。

 

膜タンパク質可溶化剤 5種セット Detergent Screening Set (first choice-II) 同仁化学研究所

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上海金畔生物科技有限公司代理日本同仁化学试剂盒全线产品，欢迎访问日本同仁化学dojindo官网了解更多信息。

膜タンパク質可溶化剤 5種セット Detergent Screening Set (first choice-II) 同仁化学研究所Detergent Screening Set (first choice-II)

07 膜タンパク質可溶化剤

Detergent Screening Set (first choice-II)

  • 膜タンパク質可溶化剤
  • デタージェント

膜タンパク質可溶化剤 5種セット

  • 製品コード
    DS06　 Detergent Screening Set (first choice-II)
容　量 メーカー希望
小売価格		 富士フイルム
和光純薬
1 set ￥17,200 343-91231
キット内容
1 set CHAPS,
n-Dodecyl-β-D-maltoside,
n-Octyl-β-D-glucoside,
Sodium cholate(purified),
MEGA-8
以上5種類の各200mg包装

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  • パンフレット 膜タンパク質可溶化剤 5種セット Detergent Screening Set (first choice-II) 同仁化学研究所 プロテオミクス関連試薬
    膜タンパク質可溶化剤 5種セット Detergent Screening Set (first choice-II) 同仁化学研究所

技術情報

応用例

・CHAPS [code: C008]  
　マス肝細胞：ミクロソーム（電気泳動） 1)
　哺乳類の培養細胞：繊維芽細胞成長因子（抽出・精製） 2)
　ウシ肝細胞：T3 結合性タンパク質（抽出・精製） 3)
　NG108-15, Hybrid cell：Opiate recepters（抽出・精製） 4)
　リンパ球：5'-ヌクレオチターゼ（抽出・精製） 5)
　anti-GST antibody,etc.（非特異的吸着防止[SPR]） 6)
　Hepatic RHE（単離） 7)
　bacteriorhodospin（抽出） 8)
　マウス肝細胞：プロラクチンレセプター（抽出） 9)

・n-Dodecyl-β-D-maltoside [code: D316]
　グラム陰性菌：tetracyclin cation/proton antiporter（抽出・精製） 10)
　ウシ心臓ミトコンドリア：ATP synthase（結晶化） 11)
　Paracoccus denitrificans：nitric oxide reductase BC complex（抽出・精製） 12)
　大腸菌：Na+/H+ antiporter（抽出・精製） 13)

・n-Octyl-β-D-glucoside [code: O001]
　Trypanosoma cruzi：Trypanothione reductase（結晶化） 14)
　Rhodobacter sphaeroids：reaction center（結晶化） 15)
　Thermus thermophilus HB8：DNA excision repair enzyme UvrB（結晶化） 16)
　ヒト：17β-hydroxysteroid dehydrogenase (17β-HSD1)（結晶化） 17)
　大腸菌：SecE（抽出・精製） 18)
　大腸菌：ラクトース輸送担体（抽出） 19,20)
　HL-60,HL-60R：PKC（抽出） 21)
　ラット肺胞：SP-A, SP-D（抽出） 22)

・MEGA-8 [code: M014]
　Trypanosoma cruzi：Trypanothione reductase（結晶化）14)

参考文献

参考文献を表示する

1) G. H. Perdew, H. W. Schaup and D. P. Selivonchick, "The Use of a Zwitterionic Detergent in Two-dimensional Gel Electrophoresis of Trout Liver Microsomes", Anal. Biochem, 1983, 135, 453.
2) Y. Matuo, N. Nishi, Y. Muguruma, Y. Yoshitaka, Y. Masuda, K. Nishikawa and F. Wada, "The Usefulness of CHAPS as a Non-cytotoxic Stabilizing Agent in Purification of Growth Factors", Cytotechnology, 1998, 1, 309.
3) R. Horiuchi, K Yamauchi, H. hayashi, S. Koya, Y. Takeuchi, K. Kato, M. Kobayashi and H. Takikawa, "Purification and Characterization of 55-kDa Protein with 3,5,3'-Triiodo-L-thyronine-binding Activity and Protein Disulfide-isomerase Activity from Beef Liver Membrane", Eur. J. Biochem., 1989, 183, 529.
4) W. F. Simods, G. Koski, R. A. Streaty, L. M. Hjelmeland and W. A. Klee, "Solubilization of Avtive Opiate Receptors", Proc. Natl. Acad Sci.USA, 1980, 77, 4623.
5) M. T. Lehto and F. J. Sharom, "Release of the Glycosylphoaphatidylinositol-anchoroesd Enzyme Ecto-5'-Nucleotidase by Phopholipase C: Catalytic Activation and Modulation by the Lipid Bilayer", Biochem. J., 1998, 332, 101.
6) K. Andersson, M. Hamalainen and M. Malmqvist, "Identification and Optimaization of Regeneration Conditions for Affinity-based Biosensor Assays. A Multivariate Cocktail Approach", Anal. Chem., 1999, 71, 2475.
7) R. Scindler, R. Mentlein and W. Feldheim, "Purification and Characterization of Retinyl Ester Hydrolase as a Member of the Non-specific Carboxylesterase Supergene Family", Eur. J. Biochem., 1998, 251, 863.
8) J. Cladera, J. L. Rigaud, J. Villaverde and M. Dunach, "Liposome Solubilization and Membrane Protein Reconstitution Using Chaps and Chapso", Eur. J. Biochem., 1997, 243, 798.
9) D. S. Liscia, T. Alhadi and B. K. Vonderhaae, "Solubilization of Active Prolactin Receptors by a Nondenaturing Zwitterionic Detergent", J. Biol. Chem., 1982, 257, 9401.
10) C. Yin, M. L. A. Ramos, M. I. B. Walmsley, R. W. Taylor, A. R. Walmsley, S. B. levu and P. A. Bullough, "The Quarternary Molecular Architecture of TetA, A Secondary Tetracycline Transporter from Escherichia Coli", Molecular Microbiology, 2000, 38, 482 .
11) R. Lutter, M. Saraste, H. S. Walraven, M. J. Runswick, M. Finel, J. F. Deatherage and J. E. Walker, "F1F0-ATP Synthase from Bovine Heart Mitochndria: Development of the Purification of a Monodisperse Oligomycin-sensitivr ATPase", Biochem. J., 1993, 295, 799.
12) J. Hendriks, A. Warne, U. Gohlke, T. Haltia, C. Ludovici, M. Lubben and M. Saraste, "The Active Site of the Bacterial Nitric Cxide Reductase Is a Dinuclear Iron center", Biochemistry, 1998, 37, 13102.
13) K. A. Williams, U. Geldmacher-Kaufer, E. Padan, S. Schuldiner and W. Kuhlbrandt, "Projection Structure of NhaA, a Secondary Transporter from Escherichia Coli, at 4.0Å Resolution", EMBO J., 1999, 18, 3558.
14) R. L. Krauth-Siegel, C. Sticherling, I. Jost, C. T. Walsh, E. F. Pai, W. Kabsch and B. Lantwin, "Crystallization and Preliminary Crystallographic Analysis of Trypanothione Reductase from Trypanosoma Cruzi, the Causative Agent of Chagas' Disease", FEBS Lett., 1993, 317, 105.
15) J. P. Allen, "Crystallization of the Reaction Center from Rhodobacter Sphaeroides in a New Tetragonal form", Proteins, 1994, 20, 283.
16) A. Shibata, N. Nakagawa, M. Sugahara, R. Masui, R. Kato, S. Kuramitsu and K. Fukuyama, "Crystallization and Preliminary X-ray Diffraction Studies of a DNA Excision Repair Enzyme, UvrB, from Thermus thermophilus HB8", Acta Cryst., 1999, D55, 704.
17) S. X. Lin, D. W. Zhu, A. Azzi, R. L. Campbell, R. Breton, F. Labrie, D. Ghosh, V. Pletnev, W. L. Duax and W. Pangborn, "Studies on the Three-dimensional Structure of Estrogenic 17 beta-hydroxysteroid Dehydrogenase", J. Endocrinol., 1996, 150, S13.
18) H. Tokuda, J. Akimaru, S. Matsuyame, K. Nishiyama and S. Mizushima, "Purification of SecE and Reconctitution of SecE-dependent Protein Translocation Activity", Fed. Eur. Biochem. Soc., 1991, 279, 233.
19) 土屋友房,“膜タンパク質の可溶化と界面活性剤”, 化学と生物実験ライン5, 廣川書店, 1990.
20) M. J. Newman, D. L. Foster, T. H. Wilson and H. R. Kaback, "Purification and Reconstitution of Functional Lactose Carrier From Escherichia Coli", J. Biol. Chem., 1981, 256, 11804.
21) M. Nishikawa, F. Komada, Y. Uemura, H. Hidaka and S. Shirakawa, "Decreased Expression of Type II Protein Kinase C in HL-60 Variant Cells Resistant to Cell Differentiation by Phorbol Diester", Cancer Res., 1990, 50, 621.
22) J. R. Wright, D. F. Zlogar, J. C. Taylor, T. M. Zlogar and C. I. Restrepo, "Effects of Endotoxin on Surfactant Protein A and D Stimulation of NO Production by Alveolar Macrophages", Am. J. Physiol., 1999, 276, 650.

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膜タンパク質可溶化剤 5種セット Detergent Screening Set (first choice-II) 同仁化学研究所

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