DAKO（丹科）Human Chromogranin A clone DAK-A3抗体Monoclonal Mouse Anti-HumanChromogranin AClone DAK-A3Labels cells of neuroendocrine origin. Anti-Chromogranin A, clone DAK-A3, reacts with an epitope o
Labels cells of neuroendocrine origin. Anti-Chromogranin A, clone DAK-A3, reacts with an epitope on the C-terminal half of the chromogranin A molecule. Results aid in the classification of neuroendocrine-derived tumors.
BD human CD8抗体 Clone RPA-T8宿主物种：mouse反应物种：human应用：流式现货供应
详细介绍
BD human CD8抗体 Clone RPA-T8
Alternative Name：CD8α; CD8A; CD8 alpha; Leu2; MAL; T8; p32
BD human CD8抗体 Clone RPA-T8
Reactivity
Human (QC Testing) Rhesus, Cynomolgus, Baboon (Tested in Development)
Application
Flow cytometry (Routinely Tested)
Immunogen
Human CD8a
Workshop No.
IV T171; V T-CD08.03; VI 6T-CD8.1, 6T-081
Storage Buffer
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
DESCRIPTION
The RPA-T8 monoclonal antibody specifically binds to CD8 alpha (CD8α) . CD8α is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily. CD8α is expressed by the majority of thymocytes, by subpopulations of αβ T cells and γδ T cells and by some NK cells. Cell surface CD8α is expressed either as a disulfide-linked homodimer (CD8αα) or as a heterodimer (CD8αβ) when disulfide-bonded to a CD8 beta chain (CD8β) . CD8-positive αβ T cells coexpress both CD8αα homodimers and CD8αβ heterodimers whereas some γδ T cells and NK cells express CD8αα homodimers. CD8 plays important roles in T cell activation and selection. The extracellular IgSF domain of CD8 α binds to a non-polymorphic determinant on HLA class I molecules (α3 domain) and enables CD8 to function as a co-receptor with MHC class I-restricted TCR during T cell recognition of antigen. The cytoplasmic domain of CD8α associates with Lck, a Src family protein tyrosine kinase that is involved in intracellular signaling. The RPA-T8 and HIT8a monoclonal antibodies are not cross-blocking. This clone has been reported to react with a subset of peripheral blood lymphocytes, but not monocytes nor granuloyctes, of baboon and both rhesus and cynomolgus macaque monkey. In general, a higher frequency of CD8+ and CD4+CD8+ lymphocytes are observed in non-human primates compared to normal human donors.
FORMAT
Format
FITC
Excitation Source
Blue 488 nm
Excitation Max
494 nm
Emission Max
520 nm
FITC, fluorescein isothiocyanate, is a fluorochrome with a molecular weight of 389 Da. FITC is sensitive to pH changes and photobleaching. Due to nearly identical excitation and emission properties but different spillover characteristics, FITC and Alexa Fluor® 488 cannot be used simultaneously. FITC is relatively dim and should be reserved for highly expressed markers whenever possible.
Other Formats →
APC
APC-Cy™7
APC-R700
Alexa Fluor® 488
Alexa Fluor® 647
Alexa Fluor® 700
BB515
BUV395
BUV496
BUV563
BV421
BV510
BV650
BV711
BV786
Biotin
NA/LE
PE
PE-CF594
PE-Cy™5
SUGGESTED COMPANION PRODUCTS
Cat No.
Description
Size
555748
FITC Mouse IgG1, κ Isotype Control RUO
100 Tests
RESOURCES & TOOLS
Spectrum Viewer
Panel Designer
Download TDS
PREPARATION AND STORAGE
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.
PRODUCT NOTICES
This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
An isotype control should be used at the same concentration as the antibody of interest.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Species testing during development may have been performed with a different format of the same clone. Selected applications have been tested for cross-reactivity.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences。。ｃｏｍ/colors.
Please refer to www.bdbiosciences。。ｃｏｍ/pharmingen/protocols for technical protocols.
Replacement Information 05-321 is a recommended replacement for MAB3090
millipore Phosphotyrosine Antibody, clone 4G10抗体
重要规格表
Species Reactivity
Key Applications
Host
Format
Antibody Type
A
ICC, WB, IP, IHC
M
Purified
Monoclonal Antibody
Description
Catalogue Number
05-321
Replaces
MAB3090
Brand Family
Upstate
Trade Name
Upstate
Description
Anti-Phosphotyrosine Antibody, clone 4G10?
Background Information
Some of the tyrosine residues can be tagged with a phosphate group (phosphorylated) by protein kinases. (In its phosphorylated state, it is referred to as phosphotyrosine.). Tyrosine phosphorylation is considered as one of the key steps in signal transduction and regulation of enzymatic activity. The advent of anti-phosphotyrosine antibodies is one of significant events in signal transduction research. Before the availability of anti-phosphotyrosine antibodies, tyrosyl phospyhorylation of proteins and enzymes was investigated through hazardous and time-consuming radioactive experiments. Anti-phosphotyrosine antibodies are commonly used in western blots after the targeted proteins have been immunoprecipitated to measure the tyrosyl phosphorylation of the proteins. Anti-phosphotyrosine antibodies are also directly used on cell lysate to examine the overall change of tyrosine phosphorylation level in reponse to various treatments.
Product Information
Format
Purified
Control
Untreated A-431 (negative control) and EGF treated A-431 (positive control) whole cell lysates. Included Positive Antigen Control: Catalog # 12-302, EGF-stimulated A431 cell lysate is provided as a free positive antigen control for western immunoblotting. Aliquot as desired, refreeze immediay, and store at -20°C. The lysate is stable for 6 months at -20°C. Before use, add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for immunoblot analysis.
Presentation
0.1M Tris-Glycine, 0.15M NaCl, 0.05% Sodium Azide, pH 7.4. Liquid at 2-8°C. IgG2bκ mouse monocolonal antibody produced in vitro by mouse-mouse hybridoma 4G10? (FOX-NY [NS-1 derivative] myeloma x spleen cells).
Applications
Application
Anti-Phosphotyrosine Antibody, clone 4G10 detects tyrosine phosphorylated proteins in all species. This unique monoclonal antibody is validated for use in IC, IH, IP, WB and is backed by hundreds of publications
Key Applications
Immunocytochemistry
Western Blotting
Immunoprecipitation
Immunohistochemistry
Application Notes
Immunoprecipitation: 2-4 μg of a previous lot immunoprecipitated quantitatively the phosphotyrosine containing proteins in the lysate of a confluent culture (10 cm dish) of cells expressing an activated tyrosine kinase. To preserve phosphotyrosine, add: 0.2 mM sodium orthovanadate to the lysis buffer.
Biological Information
Immunogen
Phosphotyramine-KLH
Clone
4G10?
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Specificity
This antibody recognizes tyrosine-phosphorylated proteins from all species.
Isotype
IgG2bκ
Species Reactivity
All
Antibody Type
Monoclonal Antibody
Modifications
Phosphorylation
Purification Method
Protein G-Sepharose? Chromatography
Molecular Weight
Dependent upon the molecular weight of the tyrosine phosphorylated protein being detected.
Product Usage Statements
Quality Assurance
Routinely evaluated on EGF-treated human A431 carcinoma cells.
Western Blot Analysis: 0.5-2 μg/mL of this lot detected tyrosine-phosphorylated proteins in a modified RIPA lysate from EGF-treated human A431 carcinoma cells (Cohen, B., 1990; , Druker, B. J., 1989; Kanakura, Y., 1991).
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at 2-8°C from date of receipt.
NOTE: DO NOT FREEZE. For maximum recovery of the product, centrifuge the original vial prior to removing the cap. If the product has accidentally been frozen and thawed, spin it at 13,000 x g for 10 minutes at 2-8oC. Save the supernatant for application.
Lee, C. et al. Generation of cDNA probes directed by amino acid sequence: cloning of urate oxidase. Science 239 (1988).
Parmentier, M. et al. Molecular cloning of the thyrotropin receptor. Science 246 (1989).
Schuchman, E. H., Jackson, C. E. & Desnick, R. J. Human arylsulfatase B: MOPAC cloning, nucleotide sequence of a full-length cDNA, and regions of amino acid identity with arylsulfatases A and C. Genomics 6, 149-158 (1990).
Vallins, W. J. et al. Molecular cloning of human cardiac troponin I using polymerase chain reaction. FEBS Lett. 270, 57-61 (1990).
Wilks, A. F., Kurban, R. R., Hovens, C. M. & Ralph, S. J. The application of the polymerase chain reaction to cloning members of the protein tyrosine kinase family. Gene 85, 67-74 (1989).
Lee, C. et al. Generation of cDNA probes directed by amino acid sequence: cloning of urate oxidase. Science 239 (1988).
Parmentier, M. et al. Molecular cloning of the thyrotropin receptor. Science 246 (1989).
Schuchman, E. H., Jackson, C. E. & Desnick, R. J. Human arylsulfatase B: MOPAC cloning, nucleotide sequence of a full-length cDNA, and regions of amino acid identity with arylsulfatases A and C. Genomics 6, 149-158 (1990).
Vallins, W. J. et al. Molecular cloning of human cardiac troponin I using polymerase chain reaction. FEBS Lett. 270, 57-61 (1990).
Wilks, A. F., Kurban, R. R., Hovens, C. M. & Ralph, S. J. The application of the polymerase chain reaction to cloning members of the protein tyrosine kinase family. Gene 85, 67-74 (1989).
Lee, C. et al. Generation of cDNA probes directed by amino acid sequence: cloning of urate oxidase. Science 239 (1988).
Parmentier, M. et al. Molecular cloning of the thyrotropin receptor. Science 246 (1989).
Schuchman, E. H., Jackson, C. E. & Desnick, R. J. Human arylsulfatase B: MOPAC cloning, nucleotide sequence of a full-length cDNA, and regions of amino acid identity with arylsulfatases A and C. Genomics 6, 149-158 (1990).
Vallins, W. J. et al. Molecular cloning of human cardiac troponin I using polymerase chain reaction. FEBS Lett. 270, 57-61 (1990).
Wilks, A. F., Kurban, R. R., Hovens, C. M. & Ralph, S. J. The application of the polymerase chain reaction to cloning members of the protein tyrosine kinase family. Gene 85, 67-74 (1989).
