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C27H39N4NaO6
538.61
特点:
● 可用于流式细胞仪、共聚焦显微镜
● 操作简便
性质
它是Bis-oxonol型阴离子膜电位敏感染料,随着细胞膜去极化,其在细胞质中的分布增加,荧光增强。 据报道,在BICR / M1R-k细胞(大鼠乳腺癌细胞)中,在-25至-90mV的膜电位范围内获得了约1%/ mV的荧光强度变化。 与电势变化相关的响应遵循一阶速率方程,其速率常数为0.1至0.8 / min。
由于可以被Ar激光(488nm)激发,因此可以用于流式细胞术,共聚焦显微镜等。 除膜电位测量外,还有在细胞活力和离子通道活性评估中应用的实例。 它也可以用于高通量筛选(HTS)细胞反应。
*有关使用方法,请参照操作说明书。
参考文献
1) D. E. Epps, M. L. Wolfe and V. Groppi, “Characterization of the Steady-state and Dynamic Fluorescence Properties of the Potential-sensitive Dye Bis-(1,3-dibutylbarbituric acid)trimethine oxonol(Dibac4(3)) in Model Systems and Cells”, Chem. Phys. Lipids, 1994, 69, 137.
2) T. Braner, D. F. Hulser and R. J. Strasser, “Comparative Measurements of Membrane Potentials with Microelectrodes and Voltage-sensitibe Dyes”, Biochim. Biophys. Acta, 1984, 771, 208.
3) T. T. Rohn, A. Sauvadet, C. Pavoine and F. Pecker, “Xanthine Affects [Ca2+]i and Contractile Responses of Ventricular Cardiocytes to Electrical Stimulation”, Am. J. Physiol., 1997, 273, C909.
4) D. J. Mason, R. Allman, J. M. Stark and D. Lloyd, “Rapid Estimation of Bacterial Antibiotic Susceptibility with Flow Cytometry”, J. Microsc., 1994, 176, 8.
5) U. Langheinrich and J. Daut, “Hyperpolarization of Isolated Capillaries from Guinea-pig Heart Induced by K+ Channel Openers and Glucose Deprivation”, J. Physiol., 1997, 502, 397.
6) V. Dall’Asta, R. Getti, G. Orlandini, P. A. Rossi, B. M. Rotoli, R. Sala, O. Bussolati and G. C. Gazzola, “Membrane Potential Changes Visualized in Complete Growth Media through Confocal Laser Scanning Microscopy of bis-Oxonol-loaded Cells”, Exp. Cell Res., 1997, 231, 260.
7) K. S. Schroeder and B. D. Neagle, “FLIPR: A New Instrument for Accurate, High Throughput Optical Screening”, J. Biomol. Screening, 1996, 1, 75.
规格性状
规格性状:
本品为橙红色至红色粉末。
纯度(HPLC):98.0%或更高
二甲基亚砜可溶:测试合格性
摩尔消光系数:140,000或更高(约493 nm)
红外光谱:测试合格
处理条件
1.保存方法:冷藏