Thermo High-Capacity cDNA 反转录试剂盒4368814

Thermo High-Capacity cDNA 反转录试剂盒

  • 产品型号:  4368814
  • 简单描述
  • Thermo High-Capacity cDNA 反转录试剂盒内容与储存• 10X RT 缓冲液,1 mL• 10X RT 随机引物,1 mL• 25X dNTP 混合物,0.2 mL (100 mM)• MultiScribe 逆转录酶,0.1 mL (50 U/μL)储存在 –25°C 至 –15°C 下。
详细介绍

Thermo High-Capacity cDNA 反转录试剂盒

High Capacity cDNA 反转录试剂盒包含 cDNA 合成所需的所有组分,可单次在 20μL 反应体系中将多达 2μg 总 RNA 转化为单链 cDNA。反应体系可扩大至 100μL,在单次反应中可获得 10μg cDNA。下游实验应用包括实时荧光定量 PCR、标准 PCR 和基因芯片研究。该试剂盒是获得 cDNA 的理想选择。

  • 在实时荧光定量 PCR 中的线性靶标扩增

  • 相比其它 cDNA 合成试剂盒,具有更高的得率、准确度以及更低的成本

  • 动态范围比其它试剂盒大10倍

  • 最佳反应温度:37°C

  • 最终产物大小:长达 7kb

  • 针对多种模板进行了广泛验证

  • 使用随机引物,可对所有种类 RNA 进行第一链 cDNA 合成。该试剂盒已针对多种 RNA 模板进行了广泛验证,包括富含 GC 和 AU 的靶标,以及低水平表达的 RNA。此外,还可通过 cDNA 的体外转录中高效生成 cRNA

PCR & 实时荧光定量 PCR、反转录

仅供科学研究使用,不可用于临床诊断。

Thermo High-Capacity cDNA 反转录试剂盒


内容与储存

• 10X RT 缓冲液,1 mL
• 10X RT 随机引物,1 mL
• 25X dNTP 混合物,0.2 mL (100 mM)
• MultiScribe 逆转录酶,0.1 mL (50 U/μL)

储存在 –25°C 至 –15°C 下。


THERMO代理pierce High Capacity Endotoxin Removal Res88270

THERMO代理pierce High Capacity Endotoxin Removal Res

  • 产品型号:  88270
  • 简单描述
  • THERMO代理pierce High Capacity Endotoxin Removal ResinThermo Scientific Pierce High Capacity Endotoxin Removal Resin binds to reduce endotoxin levels in protein samples by ≥99% in 1 hour while maximiz
详细介绍

THERMO代理pierce High Capacity Endotoxin Removal Resin

现货供应,详细产品信息:

Features of High Capacity Endotoxin Removal Resin:

• High capacity—bind up to 2,000,000 EU/mL to eliminate >99% of endotoxins
• Durable—re-use resin up to 10 times with no loss in performance
• Selective—recover ≥85% of your protein sample
• High performance—meets FDA guidelines by reducing final EU concentration to <5 EU/mL
• Economical—large-volume discounts available

The Pierce High Capacity Endotoxin Removal Resin combines porous cellulose beads and an FDA-approved food preservative, poly(ε-lysine), as an affinity ligand to selectively bind endotoxins. Endotoxin levels in protein samples are reduced by ≥99% in as little as 1 hour using our spin cup format, and protein recovery is ≥85%. The modified polylysine affinity ligand eliminates the toxicity concerns associated with alternative technologies using polymixin B ligands and sodium deoxycholate buffers.

THERMO代理pierce High Capacity Endotoxin Removal Resin

规格

Format: Bottle(s)
Column Type: Affinity, Proprietary Resin
Product Size: 10 mL
Immobilized Phase: Proprietary Ligand

内容及储存

Upon receipt store at 4°C.