日本同仁化学Hampton蛋白结晶试剂盒Tris Buffer/HR2-589/HR2-725- DOJINDO

上海金畔生物科技有限公司代理日本同仁化学 DOJINDO代理商全线产品,欢迎访问官网了解更多信息

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等,我们将竭诚为您服务,欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。

Products > Optimize Reagents > Optimize – Buffers > Tris Buffer

Tris Buffer

Hampton蛋白结晶试剂盒Tris Buffer/HR2-589/HR2-725

CAT NO

HR2-589

NAME

1.0 M Tris

DESCRIPTION

100 mL

PRICE

$60.00

CAT NO

HR2-725

NAME

1.0 M Tris pH 8.5

DESCRIPTION

100 mL

PRICE

$60.00

应用

  • Crystallization grade Tris for formulating screens or for optimization
  • 结晶级 Tris 用于配制筛选或优化

特征

  • Sterile filtered
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
  • 无菌过滤
    在 1+ 型超纯水中配制:25°C 时电阻率为 18.2 兆欧厘米,总有机碳 < 5 ppb,无细菌(<1 细菌 (CFU/ml)),无热原(<0.03 内毒素 (EU/ml)) , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述Tris

Synonyms: Trizma base or 2-Amino-2-(hydroxymethyl)-1,3-propanediol or THAM or Tris base or Tris(hydroxymethyl)aminomethane or Trometamol
C4H11NO3
NH2C(CH2OH)3
Mr 121.14
CAS Number [77-86-1]
EC Number 201-064-4
Beilstein Registry Number 741883
Merck 14,9772
RTECS TY2900000
MDL Number MFCD00004679
PubChem Substance ID 24889854
Purity 99.8 – 100.1%
pKa = 8.1 at 25°C

Useful pH range of Tris: 7.0 – 9.0

Titrate HR2-589 to the desired pH using HCl
HR2-589 Measured pH Range: 10.9 – 11.1 at 25°C
HR2-589 Measured Conductivity Range: 193.3 – 236.7 µS/cm at 25°C
HR2-589 Measured Refractive Index: 1.34991 – 1.35058 at 20°C

HR2-725 is titrated to pH 8.5 using HCl
HR2-725 Measured Conductivity Range: 18.1 – 22.7 mS/cm at 25°C
HR2-725 Measured Refractive Index: 1.35310 – 1.35369 at 20°C

同义词: Trizma 碱或 2-Amino-2-(羟甲基)-1,3-丙二醇或 THAM 或 Tris 碱或 Tris(羟甲基)氨基甲烷或氨丁三醇
C4H11NO3
NH2C(CH2OH)3
121.14 先生
CAS 编号 [77-86-1]
欧盟编号 201-064-4
贝尔斯坦注册号 741883
默克 14,9772
RTECS TY2900000
MDL 编号 MFCD00004679
PubChem 物质 ID 24889854
纯度 99.8 – 100.1%
pKa = 8.1 在 25°C

Tris 的有用 pH 值范围:7.0 – 9.0

使用 HCl 将 HR2-589 滴定至所需的 pH 值
HR2-589 测得的 pH 范围:25°C 时为 10.9 – 11.1
HR2-589 测量的电导率范围:25°C 时为 193.3 – 236.7 µS/cm
HR2-589 实测折射率:20°C 时为 1.34991 – 1.35058

使用 HCl 将 HR2-725 滴定至 pH 8.5
HR2-725 测量的电导率范围:25°C 时为 18.1 – 22.7 mS/cm
HR2-725 实测折射率:20°C 时为 1.35310 – 1.35369

Insoluble Matter 0.005% max
DNase None Detected
RNase None Detected
Protease None Detected
Heavy Metals 5 ppm max
As  ≤ 5 ppm max
Ca  ≤ 5 ppm max
Cu  ≤ 5 ppm max
Fe  ≤ 5 ppm max
Pb  ≤ 5 ppm max
Mg ≤ 5 ppm max

Trizma is a registered trademark of Sigma-Aldrich Co.

支持材料

Hampton蛋白结晶试剂盒Tris Buffer/HR2-589/HR2-725 HR2-589 Tris SDSHampton蛋白结晶试剂盒Tris Buffer/HR2-589/HR2-725 HR2-725 Tris pH 8.5 SDSHampton蛋白结晶试剂盒Tris Buffer/HR2-589/HR2-725 Titration Table

相关项目

  • Hydrochloric acid
  • StockOptions Tris Buffer Kit
  • Individual StockOptions Tris Reagents
  • 盐酸
    StockOptions Tris 缓冲套件
    个人股票期权 Tris 试剂

参考

1. Purification and crystallization of Pseudomonas aeruginosa chloramphenicol acetyltransferase. Tian, Y; Beaman, TW; Roderick, SL; Proteins 28, 298- 300, 1997.

1. 铜绿假单胞菌氯霉素乙酰转移酶的纯化与结晶。 田,Y; 比曼,台湾; 罗德里克,SL; 蛋白质 28, 298-300, 1997。

日本同仁化学Hampton蛋白结晶试剂盒ADA Buffer- DOJINDO

上海金畔生物科技有限公司代理日本同仁化学 DOJINDO代理商全线产品,欢迎访问官网了解更多信息

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等,我们将竭诚为您服务,欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。

Products > Optimize Reagents > Optimize – Buffers > ADA Buffer

ADA Buffer

应用

  • Crystallization grade ADA buffer for formulating screens or for optimization
  • 用于配制筛选或优化的结晶级 ADA 缓冲液

特征

  • Sterile filtered 无菌过滤
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
  • 在 1+ 型超纯水中配制:25°C 时电阻率为 18.2 兆欧厘米,总有机碳 < 5 ppb,无细菌(<1 细菌 (CFU/ml)),无热原(<0.03 内毒素 (EU/ml)) , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述

ADA (Buffer)

Synonyms: N-(2-Acetamido)iminodiacetic acid;
N-(Carbamoylmethyl)iminodiacetic acid
C6H10N2O5
H2NCOCH2N(CH2CO2H)2
Mr 190.16
CAS Number [26239-55-4]
EC Number 247-530-0
Beilstein Registry Number 1787181
Merck 14,147
Purity ≥ 99.0%
MDL Number MFCD00008031
PubChem Substance ID 24845005
pKa = 6.6 at 25°Celsius

Useful pH range of ADA is 5.6 – 7.5

Titrate HR2-507 to the desired pH using NaOH
HR2-817 is titrated to pH 6.5 using NaOH

HR2-507 measured Conductivity range: 23.6 – 28.6 mS/cm at 25°C
HR2-507 measured Refractive Index range: 1.35009 – 1.35044 at 20°C

HR2-817 measured Conductivity range: 42.2 – 50.4 mS/cm at 25°C
HR2-817 measured Refractive Index range: 1.36787 – 1.36913 at 20°C

ADA buffer is used in the Hampton Research MembFac crystallization kit.ADA 缓冲液用于 Hampton Research MembFac 结晶试剂盒。

Total Impurities Insoluble matter, passes filter test总杂质 不溶物,通过过滤测试
Al  ≤0.0005%
As ≤0.00001%
Ba ≤0.0005%
Bi  ≤0.0005%
Ca ≤0.001%
Cd ≤0.0005%
Cl  ≤0.1%
Co ≤0.0005%
Cr  ≤0.0005%
Cu ≤0.0005%
Fe  ≤0.0005%
K   ≤0.005%
Mg ≤0.0005%
Mn ≤0.0005%
Mo ≤0.0005%
Na ≤0.05%
Ni  ≤0.0005%
Pb  ≤0.0005%
SO4 ≤0.005%
Sr  ≤0.0005%
Zn  ≤0.0005%

Hampton蛋白结晶试剂盒ADA Buffer

CAT NO

HR2-507

NAME

0.5 M ADA

DESCRIPTION

100 mL

PRICE

$123.00

CAT NO

HR2-817

NAME

1.0 M ADA pH 6.5

DESCRIPTION

100 mL

PRICE

$132.00

SDS

相关项目

  • Sodium hydroxide 氢氧化钠
  • Individual StockOptions ADA Reagents 个人股票期权 ADA 试剂
  • StockOptions ADA Buffer Kit StockOptions ADA 缓冲套件

参考

1. Structures of the noncovalent complexes of human and bovine prothrombin fragment 2 with human PPACK-thrombin. Arni, RK; Padmanabhan, K; Padmanabhan, KP; Wu, TP; Tulinsky, A. Biochemistry 32, 4727- 4737, 1993.

2. Structures of Three Crystals Forms of the Sweet Protein Thaumatin. Ko, TP; Day, J; Greenwood, A; McPherson, A. Acta Crystallogr D 50, 813- 825, 1994.

1.人和牛凝血酶原片段2与人PPACK-凝血酶的非共价复合物的结构。 阿尼,RK; 帕德马纳班,K; 帕德马纳班,KP; 吴,TP; Tulinsky, A. Biochemistry 32, 4727-4737, 1993。

2. 甜蛋白奇异果甜蛋白的三种晶型结构。 高,TP; 日,J; 格林伍德,一个; McPherson, A. 晶体学学报 D 50, 813-825, 1994。

日本同仁化学Hampton蛋白结晶试剂盒BICINE Buffer- DOJINDO

上海金畔生物科技有限公司代理日本同仁化学 DOJINDO代理商全线产品,欢迎访问官网了解更多信息

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等,我们将竭诚为您服务,欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。

Products > Optimize Reagents > Optimize – Buffers > BICINE Buffer

BICINE Buffer

应用

  • Crystallization grade BICINE buffer for formulating screens or for optimization
  • 用于配制屏幕或优化的结晶级 BICINE 缓冲液

特征

  • Sterile filtered
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
  • 无菌过滤
    在 1+ 型超纯水中配制:25°C 时电阻率为 18.2 兆欧厘米,总有机碳 < 5 ppb,无细菌(<1 细菌 (CFU/ml)),无热原(<0.03 内毒素 (EU/ml)) , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述 BICINE

Synonyms: N,N-Bis(2-hydroxyethyl)glycine
C6H13NO4
Mr 163.17
CAS Number [150-25-4]
EC Number 205-755-1
Beilstein Registry Number 1769362
Merck 14,1201
RTECS MB9700000
MDL Number MFCD00004295
PubChem Substance ID 24891738
Purity ≥ 99.0%
pKa 8.3 at 20°C, 8.3 at 25°C

Useful pH Range 7.4-9.3
Titrate HR2-509 to useful pH range using NaOH
HR2-509 pH Range: 5.0 – 5.3 at 25°C
HR2-509 Measured Conductivity Range: 100.7 – 167.2 µS/cm at 25°C
HR2-509 Measured Refractive Index Range: 1.35769 – 1.35821 at 20°C

HR2-723 has been titrated to pH 9.0 using NaOH
HR2-723 Measured Conductivity Range: 23.9 – 25.0 mS/cm at 25°C
HR2-723 Measured Refractive Index Range: 1.36054 – 1.36122 at 20°C

同义词: N,N-双(2-羟乙基)甘氨酸
C6H13NO4
163.17先生
CAS 编号 [150-25-4]
欧盟编号 205-755-1
贝尔斯坦注册号 1769362
默克 14,1201
RTECS MB9700000
MDL 编号 MFCD00004295
PubChem 物质 ID 24891738
纯度≥99.0%
pKa 在 20°C 时为 8.3,在 25°C 时为 8.3

有用的 pH 范围 7.4-9.3
使用 NaOH 将 HR2-509 滴定至有用的 pH 范围
HR2-509 pH 范围:25°C 时为 5.0 – 5.3
HR2-509 测量电导率范围:25°C 时为 100.7 – 167.2 µS/cm
HR2-509 测量的折射率范围:20°C 时为 1.35769 – 1.35821

HR2-723 已使用 NaOH 滴定至 pH 9.0
HR2-723 测得的电导率范围:23.9 – 25.0 mS/cm,25°C
HR2-723 测量的折射率范围:20°C 时为 1.36054 – 1.36122

BICINE

Hampton蛋白结晶试剂盒BICINE Buffer

CAT NO

HR2-509

NAME

1.0 M BICINE

DESCRIPTION

100 mL

PRICE

$104.00

CAT NO

HR2-723

NAME

1.0 M BICINE pH 9.0

DESCRIPTION

100 mL

PRICE

$104.00

相关项目

  • Sodium hydroxide
  • Individual StockOptions Bicine Reagents
  • StockOptions Bicine Buffer Kit
  • 氢氧化钠
    个别股票期权 Bicine 试剂
    StockOptions Bicine 缓冲套件

参考

1. Crystallization and preliminary X-ray diffraction studies of a fungal hydrolase from Ophiostoma novo-ulmi. M. N. Isupov, A. A. Brindley, E. J. Hollingsworth, G. N. Murshudov, A. A. Vagin and J. A. Littlechild. Acta Cryst. (2004). D60, 1879-1882.

1. 来自 Ophiostoma novo-ulmi 的真菌水解酶的结晶和初步 X 射线衍射研究。 M. N. Isupov、A. A. Brindley、E. J. Hollingsworth、G. N. Murshudov、A. A. Vagin 和 J. A. Littlechild。 晶体学报。 (2004 年)。 D60,1879-1882。

日本同仁化学Hampton蛋白结晶试剂盒BIS-TRIS Buffer- DOJINDO

上海金畔生物科技有限公司代理日本同仁化学 DOJINDO代理商全线产品,欢迎访问官网了解更多信息

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等,我们将竭诚为您服务,欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。

Products > Optimize Reagents > Optimize – Buffers > BIS-TRIS Buffer

BIS-TRIS Buffer

应用

  • Crystallization grade BIS-TRIS buffer for formulating screens or for optimization
  • 用于配制筛选或优化的结晶级 BIS-TRIS 缓冲液

特征

  • Sterile filtered
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
  • 无菌过滤
    在 1+ 型超纯水中配制:25°C 时电阻率为 18.2 兆欧厘米,总有机碳 < 5 ppb,无细菌(<1 细菌 (CFU/ml)),无热原(<0.03 内毒素 (EU/ml)) , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述 BIS-TRIS

Synonyms: 2,2-Bis(hydroxymethyl)-2,2′,2″”-nitrilotriethanol, or 2-Bis(2-hydroxyethyl)amino-2-(hydroxymethyl)-1,3-propanediol, or Bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane.
C8H19NO5
Mr 209.24
CAS Number [6976-37-0]
EC Number 230-237-7
Beilstein Registry Number 2205275
MDL Number MFCD00002853
Purity ≥ 98.0%
pKa = 6.4 at 20°C
pKa = 6.5 at 25°C

HR2-781 is titrated to pH 5.5 using HCl
Measured Conductivity Range of HR2-781: 38.4 – 43.1 mS/cm at 25°C
Measured Refractive Index of HR2-781: 1.37069 – 1.37150 at 20°C

HR2-783 is titrated to pH 6.5 using HCl
Measured Conductivity Range of HR2-783: 22.8 – 28.7 mS/cm at 25°C
Measured Refractive Index of HR2-783: 1.36817 – 1.36926 at 20°C

同义词:2,2-双(羟甲基)-2,2′,2″”-次氮基三乙醇,或 2-双(2-羟乙基)氨基-2-(羟甲基)-1,3-丙二醇,或双(2- 羟乙基)氨基-三(羟甲基)甲烷。
C8H19NO5
209.24 先生
CAS 编号 [6976-37-0]
欧盟编号 230-237-7
贝尔斯坦注册号 2205275
MDL 编号 MFCD00002853
纯度≥98.0%
pKa = 6.4 在 20°C
pKa = 6.5 在 25°C

使用 HCl 将 HR2-781 滴定至 pH 5.5
HR2-781 的测量电导率范围:25°C 时为 38.4 – 43.1 mS/cm
HR2-781 的实测折射率:20°C 时为 1.37069 – 1.37150

HR2-783 使用 HCl 滴定至 pH 6.5
HR2-783 的测量电导率范围:22.8 – 28.7 mS/cm 在 25°C
HR2-783 的实测折射率:20°C 时为 1.36817 – 1.36926

Al  ≤0.0005%
Ca ≤0.0005%
Cl  ≤0.05%
Cu ≤0.0005%
Fe  ≤0.0005%
K   ≤0.005%
Mg ≤0.0005%
NH4 ≤0.05%
Na  ≤0.005%
Pb  ≤0.001%
SO4 ≤0.05%
Zn  ≤0.0005%

BIS-TRIS

Hampton蛋白结晶试剂盒BIS-TRIS Buffer

CAT NO

HR2-781

NAME

1.0 M BIS-TRIS pH 5.5

DESCRIPTION

100 mL

PRICE

$169.00

CAT NO

HR2-783

NAME

1.0 M BIS-TRIS pH 6.5

DESCRIPTION

100 mL

PRICE

$169.00

相关项目

  • Hydrochloric acid
  • StockOptions Bis-Tris Buffer Kit
  • Individual StockOptions Bis-Tris Reagents
  • 盐酸
    StockOptions Bis-Tris 缓冲套件
    个别股票期权 Bis-Tris 试剂

参考

1. Atomic structure of the degraded procapsid particle of the bacteriophage G4: induced structural changes in the presence of calcium ions and functional implications. McKenna, R; Bowman, BR; Ilag, LL; Rossmann, MG; Fane, BA. J Mol Biol 256, 736- 750, 1996.

2. Expression, crystallization and preliminary X-ray diffraction study of FtsY, the docking protein of the signal recognition particle of E. coli. Montoya, G; Svensson, C; Luirink, J; Sinning, I. Proteins 28, 285- 288, 1997.

1. 噬菌体 G4 降解前衣壳颗粒的原子结构:在钙离子存在下诱导结构变化和功能影响。 麦肯纳,R; 鲍曼,BR; 伊拉格,LL; 罗斯曼,MG; 法恩,文学士。 分子生物学杂志 256, 736-750, 1996。

2.大肠杆菌信号识别颗粒对接蛋白FtsY的表达、结晶和初步X射线衍射研究。 蒙托亚,G; 斯文森,C; 卢林克,J; Sinning, I. 蛋白质 28, 285-288, 1997。

日本同仁化学Hampton蛋白结晶试剂盒Citric acid Buffer- DOJINDO

上海金畔生物科技有限公司代理日本同仁化学 DOJINDO代理商全线产品,欢迎访问官网了解更多信息

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等,我们将竭诚为您服务,欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。

Products > Optimize Reagents > Optimize – Buffers > Citric acid Buffer

Citric acid Buffer

应用

  • Crystallization grade Citric acid buffer for formulating screens or for optimization
  • 用于配制筛选或优化的结晶级柠檬酸缓冲液

特征

  • Sterile filtered
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
  • 无菌过滤
    在 1+ 型超纯水中配制:25°C 时电阻率为 18.2 兆欧厘米,总有机碳 < 5 ppb,无细菌(<1 细菌 (CFU/ml)),无热原(<0.03 内毒素 (EU/ml)) , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述 Citric acid

Synonyms: Citric acid anhydrous
C6H8O7
HOC(COOH)(CH2COOH)2
Mr 192.13
CAS Number [77-92-9]
EC Number 201-069-1
Beilstein Registry Number 782061
Merck 14,2326
RTECS GE7350000
MDL Number MFCD00011669
PubChem Substance ID 24856611
Purity ≥ 99.5%
Useful pH range of citric acid as a buffer 2.2 – 6.5
pKa1 = 3.13
pKa2 = 4.76
pKa3 = 6.4

HR2-757 is titrated to pH 3.5 at 25 degrees Celsius using NaOH
Measured Conductivity Range: 28.7 – 33.3 mS/cm at 25°C
Measured Refractive Index Range: 1.36236 – 1.36329 at 20°C

别名: 无水柠檬酸
C6H8O7
HOC(COOH)(CH2COOH)2
192.13 先生
CAS 编号 [77-92-9]
欧盟编号 201-069-1
贝尔斯坦注册号 782061
默克 14,2326
RTECS GE7350000
MDL 编号 MFCD00011669
PubChem 物质 ID 24856611
纯度≥99.5%
柠檬酸作为缓冲液的有用 pH 值范围 2.2 – 6.5
pKa1 = 3.13
pKa2 = 4.76
pKa3 = 6.4

HR2-757 在 25 摄氏度下使用 NaOH 滴定至 pH 3.5
测得的电导率范围:25°C 时 28.7 – 33.3 mS/cm
测得的折射率范围:20°C 时为 1.36236 – 1.36329

Al  ≤0.0005%
As ≤0.00001%
Ba ≤0.0005%
Bi  ≤0.0005%
Ca ≤0.001%
Cd ≤0.0005%
Cl  ≤0.0005%
Co ≤0.0005%
Cr ≤0.0005%
Cu ≤0.0005%
Fe  ≤0.0005%
K   ≤0.005%
Li   ≤0.0005%
Mg ≤0.0005%
Mn ≤0.0005%
Mo ≤0.0005%
Na ≤0.005%
Ni  ≤0.0005%
Oxalate (C2O4) ≤0.05%
Pb ≤0.0005%
PO4 ≤0.0005%
SO4 ≤0.002%
Sr ≤0.0005%
Tartrate (C4H4O6) ≤0.2%
Zn ≤0.0005%

Citric acid

Hampton蛋白结晶试剂盒Citric acid Buffer

CAT NO

HR2-757

NAME

1.0 M Citric acid pH 3.5

DESCRIPTION

100 mL

PRICE

$44.00

相关项目

  • Sodium hydroxide
  • StockOptions Citric Acid Buffer Kit
  • Individual StockOptions Citric Acid Reagents
  • 氢氧化钠
    StockOptions 柠檬酸缓冲液套件
    个别股票期权柠檬酸试剂

参考

1. Crystallization and preliminary X-ray structure solution of Rhizomucor miehei aspartic proteinase. Jia, Z; Vandonselaar, M; Schneider, P; Quail, JW. Acta Crystallogr D 51, 243- 244, 1995.

1.米黑根毛霉天冬氨酸蛋白酶的结晶和初步X射线结构解。 贾,Z; 范登塞拉,M; 施耐德,P; 鹌鹑,JW。 晶体学学报 D 51, 243-244, 1995。

日本同仁化学Hampton蛋白结晶试剂盒HEPES Buffer- DOJINDO

上海金畔生物科技有限公司代理日本同仁化学 DOJINDO代理商全线产品,欢迎访问官网了解更多信息

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等,我们将竭诚为您服务,欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。

Products > Optimize Reagents > Optimize – Buffers > HEPES Buffer

HEPES Buffer

应用

  • Crystallization grade HEPES buffer for formulating screens or for optimization
  • 用于配制筛选或优化的结晶级 HEPES 缓冲液

特征

  • Sterile filtered
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)无菌过滤
    在 1+ 型超纯水中配制:25°C 时电阻率为 18.2 兆欧厘米,总有机碳 < 5 ppb,无细菌(<1 细菌 (CFU/ml)),无热原(<0.03 内毒素 (EU/ml)) , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述 HEPES

Synonyms: 2-[4-(2-Hydroxyethyl)-1-piperazinyl]ethanesulfonic acid or 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid or N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
C8H18N2O4S
Mr 238.31
CAS Number [7365-45-9]
EC Number 230-907-9
Beilstein Registry Number 883043
Merck 14,4654
RTECS TL6809000
MDL Number MFCD00006158
PubChem Substance ID 24895616
Purity ≥ 99.5%
pKa 7.5 at 25°C

Useful pH range of HEPES buffer 6.8 – 8.2

别名:2-[4-(2-Hydroxyethyl)-1-piperazine]ethanesulfonic acid or 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid or N-(2-Hydroxyethyl)piperazine-N’-(2-ethanesulfonic 酸)
C8H18N2O4S
238.31 先生
CAS 编号 [7365-45-9]
欧盟编号 230-907-9
贝尔斯坦注册号 883043
默克 14,4654
RTECS TL6809000
MDL 编号 MFCD00006158
PubChem 物质 ID 24895616
纯度≥99.5%
pKa 7.5 在 25°C

HEPES 缓冲液的有用 pH 范围 6.8 – 8.2

Titrate HR2-585 to the desired pH between 6.8 – 8.2 using NaOH
HR2-585 Measured pH Range: 5.4 – 5.5 at 25°C
HR2-585 Measured Conductivity Range: 160.1 – 236.9 μS/cm at 25°C
HR2-585 Measured Refractive Index Range: 1.37093 – 1.37131 at 20°C

HR2-785 is titrated to pH 7.0 using NaOH
HR2-785 Measured Conductivity Range: 5.8 – 7.0 mS/cm at 25°C
HR2-785 Measured Refractive Index Range: 1.37133 – 1.37176 at 20°C

HR2-729 is titrated to pH 7.5 using NaOH
HR2-729 Measured Conductivity Range: 11.6 – 13.6 mS/cm at 25°C
HR2-729 Measured Refractive Index Range: 1.37177 – 1.37290 at 20°C

Insoluble Matter 0.01% max
DNase None Detected
RNase None Detected
Protease None Detected
Heavy Metals 1 ppm max
As 5 ppm max
Cu 5 ppm max
Fe 5 ppm max
Pb 5 ppm max
SO4 0.005% max

Hampton蛋白结晶试剂盒HEPES Buffer

CAT NO

HR2-585

NAME

1.0 M HEPES

DESCRIPTION

100 mL

PRICE

$113.00

CAT NO

HR2-785

NAME

1.0 M HEPES pH 7.0

DESCRIPTION

100 mL

PRICE

$113.00

CAT NO

HR2-729

NAME

1.0 M HEPES pH 7.5

DESCRIPTION

100 mL

PRICE

$113.00

相关项目

  • Sodium hydroxide
  • StockOptions Hepes Buffer Kit
  • Individual StockOptions HEPES Reagents
  • 氢氧化钠
    StockOptions Hepes 缓冲液套件
    个别股票期权 HEPES 试剂

参考

1. Crystallization and preliminary X-ray crystallographic studies of native elastase from North Atlantic salmon (Salmo salar). Berglund, GI; Snalas, AO; Hansen, LK. Acta Crystallogr D 51, 393- 394, 1995.

1. 来自北大西洋鲑鱼 (Salmo salar) 的天然弹性蛋白酶的结晶和初步 X 射线晶体学研究。 伯格伦德,GI; 斯纳拉斯,AO; 汉森,LK。 晶体学学报 D 51, 393-394, 1995。

日本同仁化学Hampton蛋白结晶试剂盒HEPES sodium Buffer- DOJINDO

上海金畔生物科技有限公司代理日本同仁化学 DOJINDO代理商全线产品,欢迎访问官网了解更多信息

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等,我们将竭诚为您服务,欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。

Products > Optimize Reagents > Optimize – Buffers > HEPES sodium Buffer

HEPES sodium Buffer

应用

  • Crystallization grade HEPES sodium buffer for formulating screens or for optimization
  • 用于配制筛选或优化的结晶级 HEPES 钠缓冲液

特征

  • Sterile filtered
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
  • 无菌过滤
    在 1+ 型超纯水中配制:25°C 时电阻率为 18.2 兆欧厘米,总有机碳 < 5 ppb,无细菌(<1 细菌 (CFU/ml)),无热原(<0.03 内毒素 (EU/ml)) , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述HEPES sodium

Synonyms: HEPES sodium salt, or 4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt, or N-(2-hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) sodium salt
C8H17N2NaO4S
Mr 260.29
CAS [75277-39-3]
EC Number 278-169-7
MDL Number MFCD00036463
PubChem Substance ID 24895780
pKa 7.5 at 25°C
Useful pH Range of HEPES sodium: 6.6 – 8.2

别名:HEPES钠盐,或4-(2-羟乙基)哌嗪-1-乙磺酸钠盐,或N-(2-羟乙基)哌嗪-N’-(2-乙磺酸)钠盐
C8H17N2NaO4S
260.29 先生
CAS [75277-39-3]
欧盟编号 278-169-7
MDL 编号 MFCD00036463
PubChem 物质 ID 24895780
pKa 7.5 在 25°C
HEPES钠的有用pH范围:6.6 – 8.2

HR2-733 is titrated to pH 7.5 using HCl
HR2-733 measured Conductivity range: 41.7 – 43.4 mS/cm at 25°C
HR2-733 measured Refractive Index range: 1.37577 – 1.37766 at 20°C

HR2-577 is the free base of HEPES sodium and is to be titrated to the desired pH using HCl
HR2-577 measured pH Range: 10.2 – 12.1 at 25°C
HR2-577 measured Conductivity range: 25.9 – 27.2 mS/cm at 25°C
HR2-577 measured Refractive Index range: 1.37097 – 1.37298 at 20°C

HR2-931-01 – 1.0 M HEPES sodium pH 6.8 is a Custom Shop reagent and is used to reproduce and optimize Crystallization Reagents for use with the Silver Bullets kit.

使用 HCl 将 HR2-733 滴定至 pH 7.5
HR2-733 测量的电导率范围:25°C 时 41.7 – 43.4 mS/cm
HR2-733 测量的折射率范围:20°C 时为 1.37577 – 1.37766

HR2-577 是 HEPES 钠的游离碱,使用 HCl 滴定至所需的 pH 值
HR2-577 测量的 pH 范围:25°C 时为 10.2 – 12.1
HR2-577 测量的电导率范围:25.9 – 27.2 mS/cm 在 25°C
HR2-577 测量的折射率范围:20°C 时为 1.37097 – 1.37298

HR2-931-01 – 1.0 M HEPES 钠 pH 6.8 是 Custom Shop 试剂,用于复制和优化与 Silver Bullets 试剂盒配合使用的结晶试剂。

Hampton蛋白结晶试剂盒HEPES sodium Buffer

CAT NO

HR2-577

NAME

1.0 M HEPES sodium

DESCRIPTION

100 mL

PRICE

$133.00

CAT NO

HR2-733

NAME

1.0 M HEPES sodium pH 7.5

DESCRIPTION

100 mL

PRICE

$133.00

相关项目

  • Hydrochloric acid
  • StockOptions Sodium HEPES Buffer Kit
  • Individual StockOptions Sodium HEPES Reagents
  • 盐酸
    StockOptions HEPES 钠缓冲液套件
    个别股票期权 HEPES 钠试剂

参考

1. Crystallization and preliminary X-ray characterization of GM2-activator protein. C. S. Wright and S.-C. Li. Acta Cryst. (1997). D53, 211-212.

1. GM2 激活蛋白的结晶和初步 X 射线表征。 C. S. 赖特和 S.-C。 李。 晶体学报。 (1997)。 D53,211-212。

日本同仁化学Hampton蛋白结晶试剂盒Imidazole Buffer/HR2-573/HR2-819- DOJINDO

上海金畔生物科技有限公司代理日本同仁化学 DOJINDO代理商全线产品,欢迎访问官网了解更多信息

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等,我们将竭诚为您服务,欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。

Products > Optimize Reagents > Optimize – Buffers > Imidazole Buffer

Imidazole Buffer

应用

  • Crystallization grade Imidazole for formulating screens or for optimization
  • 用于配制筛选或优化的结晶级咪唑

特征

  • Sterile filtered
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
  • 无菌过滤
    在 1+ 型超纯水中配制:25°C 时电阻率为 18.2 兆欧厘米,总有机碳 < 5 ppb,无细菌(<1 细菌 (CFU/ml)),无热原(<0.03 内毒素 (EU/ml)) , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述 Imidazole

Synonyms: 1,3-Diaza-2,4-cyclopentadiene or Glyoxaline
C3H4N2
Mr 68.08
CAS Number [288-32-4]
EC Number 206-019-2
Beilstein Registry Number 103853
Merck 14,4912
RTECS NI3325000
MDL Number MFCD00005183
PubChem Substance ID 24880372
Purity ≥ 99.5%
UN Number 2923 8/PG 3

pKa = 6.95
The useful pH range of an Imidazole can be 6.2 – 7.8

HR2-819 is titrated to pH 7.0 using HCl
HR2-819 Measured Conductivity Range: 45.4 – 50.4 mS/cm at 25°C
HR2-819 Measured Refractive Index Range: 1.34836 – 1.34904 at 20°C

Titrate HR2-573 to the desired pH using HCl
HR2-573 Measured pH Range: 10.5 – 10.7 at 25°C
HR2-573 Measured Conductivity Range: 71.1 – 101.5 µS/cm at 25°C
HR2-573 Measured Refractive Index Range: 1.34420 – 1.34440 at 20°C

同义词:1,3-Diaza-2,4-cyclopentadiene 或 Glyoxaline
C3H4N2
68.08 先生
CAS 编号 [288-32-4]
欧盟编号 206-019-2
贝尔斯坦注册号 103853
默克 14,4912
RTECS NI3325000
MDL 编号 MFCD00005183
PubChem 物质 ID 24880372
纯度≥99.5%
联合国编号 2923 8/PG 3

pKa = 6.95
咪唑的有用 pH 范围可以是 6.2 – 7.8

HR2-819 使用 HCl 滴定至 pH 7.0
HR2-819 测得的电导率范围:25°C 时为 45.4 – 50.4 mS/cm
HR2-819 测量的折射率范围:20°C 时为 1.34836 – 1.34904

使用 HCl 将 HR2-573 滴定至所需的 pH 值
HR2-573 测得的 pH 范围:25°C 时 10.5 – 10.7
HR2-573 测得的电导率范围:25°C 时为 71.1 – 101.5 µS/cm
HR2-573 测量的折射率范围:20°C 时为 1.34420 – 1.34440

Ca  ≤0.001%
Cd  ≤0.0005%
Cl   ≤0.005%
Co  ≤0.0005%
Cr  ≤0.0005%
Cu  ≤0.0005%
Fe  ≤0.0005%
K   ≤0.005%
Mg ≤0.0005%
Na ≤0.005%
Ni  ≤0.0005%
Pb  ≤0.0005%
SO4 ≤0.005%
Zn  ≤0.0005%

Hampton蛋白结晶试剂盒Imidazole Buffer/HR2-573/HR2-819

CAT NO

HR2-573

NAME

1.0 M Imidazole

DESCRIPTION

100 mL

PRICE

$93.00

CAT NO

HR2-819

NAME

1.0 M Imidazole pH 7.0

DESCRIPTION

100 mL

PRICE

$111.00

相关项目

  • Hydrochloric acid
  • Individual StockOptions Imidazole Reagents
  • StockOptions Imidazole Kit
  • 盐酸
    个别股票期权 咪唑试剂
    StockOptions 咪唑试剂盒

参考

1. Structure of apo acyl carrier protein and a proposal to engineer protein crystallization through metal ions. X. Qiu and C. A. Janson. Acta Cryst. (2004). D60, 1545-1554 [doi:10.1107/S0907444904015422].

2. Protein crystallization by surface entropy reduction: optimization of the SER strategy. D. R. Cooper, T. Boczek, K. Grelewska, M. Pinkowska, M. Sikorska, M. Zawadzki and Z. Derewenda. Acta Cryst. (2007). D63, 636-645 [doi:10.1107/S0907444907010931].

1. apo 酰基载体蛋白的结构和通过金属离子设计蛋白质结晶的建议。 X. 邱和 C. A. Janson。 晶体学报。 (2004 年)。 D60, 1545-1554 [doi:10.1107/S0907444904015422]。

2. 表面熵减少的蛋白质结晶:SER 策略的优化。 D. R. Cooper、T. Boczek、K. Grelewska、M. Pinkowska、M. Sikorska、M. Zawadzki 和 Z. Derewenda。 晶体学报。 (2007 年)。 D63, 636-645 [doi:10.1107/S0907444907010931]。

日本同仁化学MES monohydrate Buffer/HR2-587 0.5 M MES monohydrate/HR2-787 1.0 M MES monohydrate pH 6.5- DOJINDO

上海金畔生物科技有限公司代理日本同仁化学 DOJINDO代理商全线产品,欢迎访问官网了解更多信息

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等,我们将竭诚为您服务,欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。

Products > Optimize Reagents > Optimize – Buffers > MES monohydrate Buffer

MES monohydrate Buffer

CAT NO

HR2-587

NAME

 0.5 M MES monohydrate

DESCRIPTION

100 mL

PRICE

$141.00

CAT NO

HR2-787

NAME

 1.0 M MES monohydrate pH 6.5

DESCRIPTION

100 mL

PRICE

$141.00

MES monohydrate Buffer/HR2-587 0.5 M MES monohydrate/HR2-787 1.0 M MES monohydrate pH 6.5

应用

  • Crystallization grade MES monohydrate for formulating screens or for optimization
  • 用于配制筛选或优化的结晶级 MES 一水合物

特征

  • Sterile filtered
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
  • 无菌过滤
    在 1+ 型超纯水中配制:25°C 时电阻率为 18.2 兆欧厘米,总有机碳 < 5 ppb,无细菌(<1 细菌 (CFU/ml)),无热原(<0.03 内毒素 (EU/ml)) , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述 MES monohydrate

Synonyms: MES or 2-(N-morpholino)ethanesulfonic acid or 4-morpholineethanesulfonic acid monohydrate
C6H13NO4S • H2O
Mr 213.25
CAS Number [145224-94-8]
EC Number 224-632-3
Beilstein Registry Number 329824236
RTECS QE3479500
MDL Number MFCD00149409
PubChem Substance ID 24885996
Purity ≥ 99.0%
pKa 6.1 at 25°C

Solutions are stable at 2-8°C for months. Autoclaving is not recommended for any sulfonic acid buffer. When MES solutions are autoclaved, they turn yellow (although pH does not change measurably). The identity of the yellow breakdown product is unknown. When MES solutions are stored at room temperature, they can turn yellow within six months.

Useful pH range of MES 5.2 – 7.1

Titrate HR2-587 to the desired pH using NaOH
HR2-587 Measured pH Range: 3.2 – 3.4 at 25°C
HR2-587 Measured Conductivity Range: 166.4 – 2440 µS/cm at 25°C
HR2-587 Measured Refractive Index Range: 1.34655 – 1.34731 at 20°C

HR2-787 has been titrated to pH 6.5 using NaOH
HR2-787 Measured Conductivity Range: 19.2 – 25.6 mS/cm at 25°C
HR2-787 Measured Refractive Index Range: 1.36113 – 1.36251 at 20°C

HR2-943-07 – MES monohydrate pH 5.8 – 1.0 M solution is a Custom Shop reagent and is used to reproduce and optimize Crystallization Reagents for use with the Silver Bullets kit.

同义词:MES或2-(N-吗啉代)乙磺酸或4-吗啉乙磺酸一水合物
C6H13NO4S • H2O
213.25 先生
CAS 编号 [145224-94-8]
欧盟编号 224-632-3
贝尔斯坦注册号 329824236
RTECS QE3479500
MDL 编号 MFCD00149409
PubChem 物质 ID 24885996
纯度≥99.0%
pKa 6.1 在 25°C

溶液在 2-8°C 下可稳定数月。不建议对任何磺酸缓冲液进行高压灭菌。当 MES 溶液经过高压灭菌时,它们会变黄(尽管 pH 值不会发生可测量的变化)。黄色分解产物的身份不明。当 MES 溶液在室温下储存时,它们会在六个月内变黄。

MES 5.2 – 7.1 的有用 pH 范围

使用 NaOH 将 HR2-587 滴定至所需的 pH 值
HR2-587 测得的 pH 范围:25°C 时为 3.2 – 3.4
HR2-587 测得的电导率范围:25°C 时为 166.4 – 2440 µS/cm
HR2-587 测量的折射率范围:20°C 时为 1.34655 – 1.34731

HR2-787 已使用 NaOH 滴定至 pH 6.5
HR2-787 测量的电导率范围:25°C 时为 19.2 – 25.6 mS/cm
HR2-787 测量的折射率范围:20°C 时为 1.36113 – 1.36251

HR2-943-07 – MES 一水合物 pH 5.8 – 1.0 M 溶液是 Custom Shop 试剂,用于重现和优化用于 Silver Bullets 试剂盒的结晶试剂。

Impurities:
Endotoxin ≤ 50 EU/g
Yeast and Mold ≤ 100 CFU/g
Bioburden ≤ 100 CFU/g
Al ≤ 5.0 ppm
As ≤ 3.0 ppm
Ba ≤ 5.0 ppm
Bi ≤ 5.0 ppm
Ca ≤ 20.0 ppm
Cd ≤ 5.0 ppm
Co ≤ 5.0 ppm
Cr ≤ 5.0 ppm
Cu ≤ 5.0 ppm
Fe ≤ 5.0 ppm
Li ≤ 5.0 ppm
Mg ≤ 5.0 ppm
Mn ≤ 5.0 ppm
Mo ≤ 5.0 ppm
Na ≤ 20.0 ppm
Ni ≤ 5.0 ppm
Pb ≤ 5.0 ppm
Sb ≤ 5.0 ppm
Zn ≤ 5.0 ppm

相关项目

  • Sodium hydroxide
  • StockOptions MES Buffer Kit
  • Individual StockOptions MES Reagents
  • 氢氧化钠
    StockOptions MES 缓冲套件
    个别股票期权 MES 试剂

参考

1. Crystal structures of mutant Pseudomonas aeruginosa p-hydroxybenzoate hydroxylases: the Tyr201Phe, Tyr385Phe, and Asn300Asp variants. Lah, MS; Palfey, BA; Schreuder, HA; Ludwig, ML. Biochemistry 33, 1555- 1564, 1994.

1. 突变铜绿假单胞菌对羟基苯甲酸羟化酶的晶体结构:Tyr201Phe、Tyr385Phe 和 Asn300Asp 变体。 拉,女士; 帕尔菲,文学士; 施罗德,HA; 路德维希,ML。 生物化学 33, 1555-1564, 1994。

Bluing Buffer 返蓝染色液 Dako试剂CS70230-2CN

Bluing Buffer 返蓝染色液 Dako试剂

  • 产品型号:  CS70230-2CN
  • 简单描述
  • Bluing Buffer 返蓝染色液 Dako试剂达科蓝色缓冲液,现成使用,H &amp; E 染色试剂,多达3000测试,1升
详细介绍

Bluing Buffer 返蓝染色液 Dako试剂

达科蓝色缓冲液,现成使用,H & E 染色试剂,多达3000测试,1升

用于体外诊断。


miltenyi美天旎autoMACS Running Buffer130-091-221

miltenyi美天旎autoMACS Running Buffer

  • 产品型号:  130-091-221
  • 简单描述
  • miltenyi美天旎autoMACS Running Buffer品牌:Miltenyi(美天旎)货号:130-091-221CAS号:详见说明书供应商:Miltenyi(美天旎)
详细介绍

miltenyi美天旎autoMACS Running Buffer

he autoMACS® Running Buffer is an optimized MACS® Separation Buffer suitable for MACS Cell Separation on the autoMACS Pro Separator and the autoMACS Separator and for manual MACS Cell Separation Systems which are not sensitive to azide.

Background information

The autoMACS Running Buffer is sterile-filtered and ready-to-use. It contains bovine serum albumin (BSA), EDTA, and 0.09% azide. It is quality-controlled for cell separation requirements. One bottle (1.5 L) is sufficient for up to 15 cycles consisting of cell separation and system rinsing using the autoMACS Instruments.

qiagen Buffer EB19086

qiagen Buffer EB

  • 产品型号:  19086
  • 简单描述
  • qiagen Buffer EB 货号19086用于洗脱核酸Buffer EB为洗脱缓冲液,适用于以下产品的实验:QIAprep、QIAquick、QIAwell、DirectPrep 96、LabelStar、MagAttract 96 Miniprep、MinElute和R.E.A.L. Prep 96。
详细介绍

qiagen Buffer EB 货号19086

用于洗脱核酸

    Buffer EB为洗脱缓冲液,适用于以下产品的实验:QIAprep、QIAquick、QIAwell、DirectPrep 96、LabelStar、MagAttract 96 Miniprep、MinElute和R.E.A.L. Prep 96。

    qiagen Buffer EB 货号19086

    详细产品信息可致电我公司销售人员

    货号19086

    相关产品订购:

    Buffer PM (500ml) 837 19083
    Buffer EB (250ml) 423 19086
    Reagent DX 663 19088
    Buffer RPW (125ml) 598 19090
    Buffer MTL (54 ml) 466 19112
    QIAGEN Proteinase K (2ml) 1180 19131
    QIAGEN Proteinase K (10 ml) 4200 19133
    QIAGEN Protease (7.5 AU) 996 19155
    QIAGEN Protease (30 AU) 3200 19157
    Collection Tubes (2-ml) 1830 19201
    VacConnectors (500) 1820 19407
    VacValves (24) 1250 19408
    QIAvac 24 Plus 4300 19413
    QIAvac Holder 1990 19418
    QIAvac Connecting System 13950 19419
    QIAvac 96 10200 19504
    Collection Microtubes (racked, 10 x 96) 2120 19560
    Collection Microtube Caps (120 x 8) 439 19566
    Tape Pads (5) 822 19570
    QIAEX II Gel Extraction Kit (150) 2340 20021
    QIAEX II Gel Extraction Kit (500) 5350 20051
    QIAEX II Suspension (1.5ml) 1840 20902
    Buffer QX1 (500ml) 1110 20912
    R.E.A.L. Prep 96 Plasmid Kit (24) 54800 26173
    QIAprep Spin Miniprep Kit (50) 1070 27104
    QIAprep Spin Miniprep Kit (250) 4100 27106
    QIAprep 2.0 Spin Columns (100) 1130 27115
    QIAprep 96 Turbo Core Kit (24) 64690 27173
    QIAprep 96 Turbo Miniprep Kit (4) 13660 27191
    QIAprep 96 Turbo Miniprep Kit (24) 77320 27193
    QIAprep 96 Plus Miniprep Kit (4) 15610 27291
    DirectPrep 96 Miniprep Kit (4) 7650 27361
    QuickLyse Miniprep Kit (100) 2420 27405
    QuickLyse Miniprep Kit (250) 5190 27406
    MinElute PCR Purification Kit (50) 1330 28004
    MinElute PCR Purification Kit (250) 6000 28006
    MinElute 96 UF PCR Purification Kit (4) 3710 28051
    MinElute 96 UF PCR Purification Kit (24) 14740 28053
    QIAquick PCR Purification Kit (50) 1100 28104
    QIAquick PCR Purification Kit (250) 5150 28106
    QIAquick 96 PCR Purification Kit (4) 10180 28181
    QIAquick 96 PCR Purification Kit (24) 40340 28183
    MinElute Reaction Cleanup Kit (50) 1600 28204
    MinElute Reaction Cleanup Kit (250) 7340 28206
    QIAquick Nucleotide Removal Kit (50) 1410 28304
    QIAquick Nucleotide Removal Kit (250) 6610 28306
    MinElute Gel Extraction Kit (50) 1550 28604
    MinElute Gel Extraction Kit (250) 7120 28606
    QIAquick Gel Extraction Kit (50) 1100 28704
    QIAquick Gel Extraction Kit (250) 5150 28706
    Strep-Tactin?Superflow Plus (2 ml) 2340 30002
    Strep-Tactin?Superflow Plus (10 ml) 9160 30004
    Strep-Tactin Superflow Plus Cartrdg(1ml) 1980 30020
    Strep-Tactin Superflow Plus Cartrdg(5ml) 6370 30060
    Ni-NTA Agarose (25 ml) 3850 30210
    Ni-NTA Agarose (100 ml) 13370 30230
    Ni-NTA Agarose (500 ml) 56230 30250
    NTA Agarose (25ml) 3950 30310
    Ni-NTA Superflow (25 ml) 4740 30410
    Ni-NTA Superflow (100 ml) 15920 30430
    Ni-NTA Superflow (500 ml) 67410 30450
    Ni-NTA Fast Start Kit (6) 4350 30600
    Ni-NTA Superflow Columns (12 x 1.5 ml) 5200 30622
    Ni-NTA Superflow Cartridges (5×1 ml) 2210 30721
    Ni-NTA Superflow Cartridges (1×5 ml) 1960 30760
    Ni-NTA Superflow Cartridges (5×5 ml) 7670 30761
    Ni-NTA Superflow Cartridges (100×5 ml) 122510 30765
    QIAamp UCP Pathogen Mini Kit (50) 4370 50214
    QIAamp DNA Blood Mini Kit (50) 1770 51104
    QIAamp DNA Blood Mini Kit (250) 7530 51106
    QIAamp DNA Blood Mini QIAcube Kit (240) 8450 51126
    QIAamp 96 DNA Blood Kit (4) 14930 51161
    QIAamp 96 DNA Blood Kit (12) 41740 51162
    QIAamp DNA Blood Midi Kit (20) 2590 51183
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    QIAamp DNA Blood Maxi Kit (50) 10880 51194
    FlexiGene DNA Kit (250) 3750 51206
    FlexiGene DNA AGF3000 Kit (640) 49630 51297
    QIAamp DNA Mini Kit (50) 2010 51304
    QIAamp DNA Mini Kit (250) 8890 51306
    QIAamp DNA Mini QIAcube Kit (240) 9340 51326
    QIAamp 96 DNA QIAcube HT Kit (5) 8790 51331
    QIAamp Fast DNA Tissue Kit, 50 1940 51404
    QIAamp DNA Stool Mini Kit (50) 2870 51504
    QIAamp Fast DNA Stool Mini Kit (50) 3190 51604
    QIAamp DNA Microbiome Kit (50) 6620 51704
    QIAamp RNA Blood Mini Kit (50) 4250 52304
    QIAamp Viral RNA Mini Kit (50) 3010 52904
    QIAamp Viral RNA Mini Kit (250) 12980 52906
    QIAamp Viral RNA Mini QIAcube Kit (240) 15660 52926
    QIAamp UltraSens Virus Kit (50) 6030 53704
    QIAamp UltraSens Virus Kit (250) 25230 53706
    QIAamp cador Pathogen Mini Kit (50) 2560 54104
    QIAamp cador Pathogen Mini Kit (250) 12210 54106
    cador Pathogen 96 QIAcube HT Kit (5) 11880 54161
    QIAamp Circulating Nucleic Acid Kit (50) 14160 55114
    QIAamp DNA Micro Kit (50) 2830 56304
    QIAamp DNA FFPE Tissue Kit (50) 2590 56404
    QIAamp DNA Investigator Kit (50) 2990 56504
    QIAamp MinElute Media Kit (50) 4860 57414
    QIAamp MinElute Virus Spin Kit (50) 3180 57704
    QIAamp MinElute Virus Vacuum Kit (50) 4060 57714
    QIAamp 96 Virus QIAcube HT Kit (5) 16960 57731
    EpiTect Bisulfite Kit (48) 2800 59104
    EpiTect 96 Bisulfite Kit (2) 9990 59110
    EpiTect Plus DNA Bisulfite Kit (48) 2250 59124
    EpiTect Whole Bisulfitome Kit (25) 4560 59203
    EpiTect MSP Kit (100) 3600 59305
    EpiTect MethyLight PCR Kit (200) 10340 59436
    EpiTect HRM PCR Kit (100) 1440 59445
    EpiTect MethyLight PCR +ROX Kit (200) 10600 59496
    EpiTect Control DNA (1000) 2730 59568
    EpiTect Control DNA, methylated (100) 2730 59655
    EpiTect Control DNA, unmethylated (100) 2730 59665
    EpiTect PCR Control DNA Set (100) 5460 59695
    EpiTect Fast 96 DNA Bisulfite Kit 10910 59720
    EpiTect Fast 96 FFPE Bisulfite Kit 19310 59740
    EpiTect Fast Bisulfite Kit (10) 847 59802
    EpiTect Fast DNA Bisulfite Kit (50) 2430 59824
    EpiTect Fast DNA Bisulfite Kit (200) 8290 59826
    EpiTect Fast FFPE Bisulfite Kit (50) 4240 59844
    EpiTect Fast LyseAll Bisulfite Kit (50) 2720 59864
    EpiTect Fast LyseAll Bisulfite Kit (200) 9270 59866

     

    qiagen缓冲液Buffer AL货号19075

    qiagen缓冲液Buffer AL货号19075

    • 产品型号:  
    • 简单描述
    • qiagen缓冲液Buffer AL货号19075Buffer AL是216 ml的裂解液,在采用QIAmp或DNeasy技术纯化DNA时使用。所有QIAamp Kits和DNeasy Tissue Kits都提供这种即用型裂解液(DNeasy 96 Tissue Kit中需要添加乙醇才可使用)。
    详细介绍

    qiagen缓冲液Buffer AL货号19075

    Cat No./ID: 19075

    Buffer AL (264 ml)

    Log in

    264 ml Lysis Buffer

    qiagen缓冲液Buffer AL货号19075

    Buffer AL是216 ml的裂解液,在采用QIAmp或DNeasy技术纯化DNA时使用。所有QIAamp Kits和DNeasy Tissue Kits都提供这种即用型裂解液(DNeasy 96 Tissue Kit中需要添加乙醇才可使用)。

    QIAamp® Fast DNA Stool Mini Kit
    For isolation of gDNA from stool samples
    The QIAamp Fast DNA Stool Mini Kit enables rapid purification of high-quality genomic DNA (human and bacterial) from
    fresh or frozen stool samples. The novel, liquid InhibitEX® Buffer included in the kit replaces cumbersome inhibitor removal
    tablets to efficiently deplete PCR inhibitors commonly present in stool samples and significantly reduce handling time.
    The QIAamp Fast DNA Stool Mini Kit affords:
    • A streamlined, faster purification protocol
    • Efficient depletion of PCR inhibitors
    • High sensitivity in downstream assays
    • An automated workflow on the QIAcube®
    Fast and convenient handling
    The QIAamp Fast DNA Stool Mini Kit streamlines gDNA purification from
    stool samples, reducing the total time needed from sample to isolated
    DNA to as little as 25 minutes. The unique formulation of the new
    InhibitEX Buffer efficiently separates PCR inhibitors from DNA and its
    liquid format is more convenient than tablets or powder. Manual sample
    handling is streamlined because extra addition of inhibitor removal
    reagents is no longer required and experiments can be scaled more
    easily. The QIAamp Fast DNA Stool Mini Kit can be automated on
    instruments like the QIAcube.
    Rapid protocols are provided to efficiently isolate human DNA or
    microbial DNA for pathogen detection. Shortly, stool is suspended in
    InhibitEX Buffer to separate inhibitors from DNA. Following a quick lysis,
    DNA binds to the QIAamp silica membrane. Any remaining inhibitors
    and contaminants are removed by washing steps and pure, intact DNA
    is then eluted from the column.
    QIAamp Fast DNA Stool Mini Kit
    Fully automatable on the QIAcube
    Sample
    Suspend in
    InhibitEx Buffer
    Digest proteins
    Bind
    Wash 2x
    Elute
    DNA ready for downstream applications

    qiagen Buffer PB DNA回收缓冲液19066

    qiagen Buffer PB DNA回收缓冲液19066

    • 产品型号:  
    • 简单描述
    • qiagen Buffer PB DNA回收缓冲液19066Buffer PB为500 ml的结合缓冲液,适用于DNA回收实验。
    详细介绍

    qiagen Buffer PB DNA回收缓冲液19066

    Cat No./ID: 19066

    Buffer PB (500 ml)

    500 ml Binding Buffer

    qiagen Buffer PB DNA回收缓冲液19066

    在分子生物学快速发展的今天,超过500,000位客户通过QIAGEN的样本制备与分析技术,获得基本的生命组成——DNA、RNA和蛋白质的生物学信息,在他们的研究领域取得突破性进展。
    在QIAGEN的产品和下,医生们能够更准确、更快速的诊断疾病,指导治疗。科学家们不断探索生命的奥秘,并将这些知识转化为疗效更好的药物。各领域专家通过分子技术进行人类身份认定、兽医学检测和食品安全检测。QIAGEN新一代的创新解决方案已在市场上创造价值,相信在若干年后其价值会更为可观。我们一直致力于让生活的改善成为可能。

    相关产品订购:

    Buffer PB (500ml) 1060 19066
    Buffer AW/AW2 (concentrate, 324 ml) 1220 19072
    Buffer AVL (155 ml) 1900 19073
    Buffer AL 1220 19075
    Buffer ATL (200ml) 1220 19076
    Buffer AE (240ml) 1220 19077
    Buffer AW1 (concentrate, 242 ml) 1220 19081
    Buffer ASL (4 x 140 ml) 1370 19082
    Buffer PM (500ml) 837 19083
    Buffer EB (250ml) 423 19086
    Reagent DX 663 19088
    Buffer RPW (125ml) 598 19090
    Buffer MTL (54 ml) 466 19112
    QIAGEN Proteinase K (2ml) 1180 19131
    QIAGEN Proteinase K (10 ml) 4200 19133
    QIAGEN Protease (7.5 AU) 996 19155
    QIAGEN Protease (30 AU) 3200 19157
    Collection Tubes (2-ml) 1830 19201
    VacConnectors (500) 1820 19407
    VacValves (24) 1250 19408
    QIAvac 24 Plus 4300 19413
    QIAvac Holder 1990 19418
    QIAvac Connecting System 13950 19419
    QIAvac 96 10200 19504
    Collection Microtubes (racked, 10 x 96) 2120 19560
    Collection Microtube Caps (120 x 8) 439 19566
    Tape Pads (5) 822 19570
    QIAEX II Gel Extraction Kit (150) 2340 20021
    QIAEX II Gel Extraction Kit (500) 5350 20051
    QIAEX II Suspension (1.5ml) 1840 20902
    Buffer QX1 (500ml) 1110 20912
    R.E.A.L. Prep 96 Plasmid Kit (24) 54800 26173
    QIAprep Spin Miniprep Kit (50) 1070 27104
    QIAprep Spin Miniprep Kit (250) 4100 27106
    QIAprep 2.0 Spin Columns (100) 1130 27115
    QIAprep 96 Turbo Core Kit (24) 64690 27173
    QIAprep 96 Turbo Miniprep Kit (4) 13660 27191
    QIAprep 96 Turbo Miniprep Kit (24) 77320 27193
    QIAprep 96 Plus Miniprep Kit (4) 15610 27291
    DirectPrep 96 Miniprep Kit (4) 7650 27361
    QuickLyse Miniprep Kit (100) 2420 27405
    QuickLyse Miniprep Kit (250) 5190 27406
    MinElute PCR Purification Kit (50) 1330 28004
    MinElute PCR Purification Kit (250) 6000 28006
    MinElute 96 UF PCR Purification Kit (4) 3710 28051
    MinElute 96 UF PCR Purification Kit (24) 14740 28053
    QIAquick PCR Purification Kit (50) 1100 28104
    QIAquick PCR Purification Kit (250) 5150 28106
    QIAquick 96 PCR Purification Kit (4) 10180 28181
    QIAquick 96 PCR Purification Kit (24) 40340 28183
    MinElute Reaction Cleanup Kit (50) 1600 28204
    MinElute Reaction Cleanup Kit (250) 7340 28206
    QIAquick Nucleotide Removal Kit (50) 1410 28304
    QIAquick Nucleotide Removal Kit (250) 6610 28306
    MinElute Gel Extraction Kit (50) 1550 28604
    MinElute Gel Extraction Kit (250) 7120 28606
    QIAquick Gel Extraction Kit (50) 1100 28704
    QIAquick Gel Extraction Kit (250) 5150 28706
    Strep-Tactin?Superflow Plus (2 ml) 2340 30002
    Strep-Tactin?Superflow Plus (10 ml) 9160 30004
    Strep-Tactin Superflow Plus Cartrdg(1ml) 1980 30020
    Strep-Tactin Superflow Plus Cartrdg(5ml) 6370 30060
    Ni-NTA Agarose (25 ml) 3850 30210
    Ni-NTA Agarose (100 ml) 13370 30230
    Ni-NTA Agarose (500 ml) 56230 30250
    NTA Agarose (25ml) 3950 30310
    Ni-NTA Superflow (25 ml) 4740 30410
    Ni-NTA Superflow (100 ml) 15920 30430
    Ni-NTA Superflow (500 ml) 67410 30450
    Ni-NTA Fast Start Kit (6) 4350 30600
    Ni-NTA Superflow Columns (12 x 1.5 ml) 5200 30622
    Ni-NTA Superflow Cartridges (5×1 ml) 2210 30721
    Ni-NTA Superflow Cartridges (1×5 ml) 1960 30760
    Ni-NTA Superflow Cartridges (5×5 ml) 7670 30761
    Ni-NTA Superflow Cartridges (100×5 ml) 122510 30765
    QIAamp UCP Pathogen Mini Kit (50) 4370 50214
    QIAamp DNA Blood Mini Kit (50) 1770 51104
    QIAamp DNA Blood Mini Kit (250) 7530 51106
    QIAamp DNA Blood Mini QIAcube Kit (240) 8450 51126
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    QIAamp 96 DNA Blood Kit (12) 41740 51162
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    QIAamp DNA Blood Maxi Kit (10) 2590 51192
    QIAamp DNA Blood Maxi Kit (50) 10880 51194

    THERMO RAPI裂解液,PIERCE RAPI Buffer货号8990089901

    THERMO RAPI裂解液,PIERCE RAPI Buffer货号89900

    • 产品型号:  89901
    • 简单描述
    • THERMO RAPI裂解液,PIERCE RAPI Buffer货号89900货号:89901 89900规格:250ml 100ml现货供应
    详细介绍

    THERMO RAPI裂解液,PIERCE RAPI Buffer货号89900,89901

    Thermo Scientific RIPA裂解及抽提缓冲液是一种受欢迎的高质量即用型细胞裂解试剂,其配方已*公开,适用于哺乳动物培养细胞。

    RIPA缓冲液的特点:

    • 方便— 即用型溶液;无需自行装配及配置组分
    • 灵活— 兼容多种应用,包括报告因子分析、蛋白分析、免疫分析及蛋白质纯化等
    • 通用型— 适用于细胞质蛋白、膜蛋白及核蛋白抽提
    • 公开配方— 不含组分,用户可*控制并掌握可能发生的兼容性问题

    THERMO RAPI裂解液,PIERCE RAPI Buffer货号89900,89901

    该款RIPA缓冲液能够高效裂解并抽提哺乳动物培养细胞中的蛋白(膜蛋白、核蛋白及胞质蛋白),包括铺板细胞及悬浮细胞,并且兼容多种应用,包括报告因子分析、Thermo ScientificBCA蛋白分析、免疫分析及蛋白纯化等。RIPA缓冲液配方还兼容Thermo Scientific Halt蛋白酶抑制混合物 (产品货号78430)及Halt磷酸酶抑制混合物 (产品货号 78420)等抑制剂,因此,可在使用前添加抑制剂防止蛋白水解并保持蛋白的磷酸化状态。

    RIPA缓冲液的名字来自于其zui起的应用方向:放射性免疫沉淀分析。如今,实验室已经很少应用同位素分析方法,但是这款裂解缓冲液的缩写还是沿用了下来。RIPA细胞裂解试剂含有3种非离子型和离子型去垢剂,因此对多种类型细胞都具有出色的蛋白抽提效率。

    ,89901

    订购信息:

    89900 100ml

    89901 250ml

    现货供应

    Lysis Buffer for Legionella酶试剂盒Takara


    Lysis Buffer for Legionella
    品牌 Code No. 产品名称 包装量 价格(元) 说明书 数量
    Takara 9181 Lysis Buffer for Legionella 50 preps ¥300 Lysis Buffer for Legionella Lysis Buffer for Legionella Lysis Buffer for Legionella
    收藏产品 加入购物车
     
    ■ 制品内容
    Lysis Buffer for Legionella 500 μl × 5
     
    ■ 制品说明
    本制品是一种从军团菌中提取DNA的试剂。使用含有表面活性剂和PCR反应抑制物的吸附树脂,通过热处理和离心进行DNA的提取。离心后在吸附树脂的上面形成凝胶层,便于DNA溶液的回收。
     
    ■ 保存
    4℃ (避免冻结)。
     
    ■ 实验操作
    1. 将Legionella菌液于15,000 rpm (最高转速) 4℃ 离心5min,弃上清液。
    2. Lysis Buffer for Legionella在使用前振荡混匀。使用前,用移液器反复吸打以确保树脂混合均匀,然后取50 μl添加至细胞沉淀中,轻微振荡混匀后离心。
    3. 95℃温育10 min。
    4. 轻微振荡混匀之后于15,000 rpm (最高转速) 4℃ 离心10 min。
    5. 冰中放置5 min。
    6. 回收上清液。一般情况下,DNA溶液的回收量大约为25 μl。
    * Lysis Buffer for Legionella中含有低浓度的凝胶,细胞裂解离心之后,会在树脂上形成肉眼不可见的凝胶层。步骤5的处理能够使凝胶层彻底凝固,所以在收集上清液时应注意从DNA溶液层表面吸取,避免触碰凝胶层。
    注意:使用本制品前请充分振荡混匀,并且在使用前反复吸打混匀以确保树脂均匀分布。如果枪头堵塞,可以剪掉枪尖。
    Lysis Buffer for Legionella
     
     

    页面更新:2020-06-15 15:33:23

    Cell Lysis Buffers酶试剂盒Takara


    Cell Lysis Buffers
    品牌 Code No. 产品名称 包装量 价格(元) 说明书 数量
    Clontech 635625 TALON xTractor Buffer 500 ml ¥4,422 Cell Lysis Buffers Cell Lysis Buffers Cell Lysis Buffers
    Clontech 635656 xTractor Buffer 100 ml ¥1,616 Cell Lysis Buffers Cell Lysis Buffers Cell Lysis Buffers
    Clontech 635671 xTractor Buffer 250 ml ¥2,302 Cell Lysis Buffers Cell Lysis Buffers Cell Lysis Buffers
    Clontech 635623 xTractor Buffer Kit 10 Extractions ¥4,447 Cell Lysis Buffers Cell Lysis Buffers Cell Lysis Buffers
    收藏产品 加入购物车

    无标题文档

     
    Code No. 产品名称
    635625   xTractor Buffer
    635656   xTractor Buffer
    635671   xTractor Buffer
    635623   xTractor Buffer Kit
     
    √    通用 可从细菌、酵母、哺乳动物细胞和杆状病毒感染的细胞中提取蛋白质
    √    高效率 效果优于sonic或其他细胞裂解液
    √    快速 流程简便,只需10min孵育
    √    温和 非变性提取条件,更有利于保持生物活性
    √    兼容 适用于所有IMAC树脂,快速纯化标签蛋白质
     
    Cell Lysis Buffers
    快速纯化His标签蛋白操作流程图
     
    产品详情请点击:Cell Lysis Buffers
     
     

    页面更新:2019-11-11 16:13:11

    磁珠法免疫沉淀 Buffer Set酶试剂盒Takara


    磁珠法免疫沉淀 Buffer Set
    品牌 Code No. 产品名称 包装量 价格(元) 说明书 数量
    Clontech 635696 Magnetic Beads Immunoprecipitation Buffer Set 50 Rxns ¥2,288 磁珠法免疫沉淀 Buffer Set 磁珠法免疫沉淀 Buffer Set 磁珠法免疫沉淀 Buffer Set
    收藏产品 加入购物车

    无标题文档

     
    Code No. 产品名称
    635696   Magnetic Beads Immunoprecipitation Buffer Set
     
    ■ 产品描述
    本制品由裂解液、洗涤buffer和洗脱buffer组成,适用于磁珠法免疫沉淀蛋白质的纯化,例如我们的Anti-Myc 磁珠和Anti-DYKDDDDK(FLAG)磁珠(已终卖)。
     
    ■ 应用
    免疫沉淀
     
     
    产品详情请点击:磁珠法免疫沉淀 Buffer Set
     
     

    页面更新:2019-11-12 09:41:38

    磁珠法免疫沉淀 Buffer Set酶试剂盒Takara


    磁珠法免疫沉淀 Buffer Set
    品牌 Code No. 产品名称 包装量 价格(元) 说明书 数量
    Clontech 635696 Magnetic Beads Immunoprecipitation Buffer Set 50 Rxns ¥2,288 磁珠法免疫沉淀 Buffer Set 磁珠法免疫沉淀 Buffer Set 磁珠法免疫沉淀 Buffer Set
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    Code No. 产品名称
    635696   Magnetic Beads Immunoprecipitation Buffer Set
     
    ■ 产品描述
    本制品由裂解液、洗涤buffer和洗脱buffer组成,适用于磁珠法免疫沉淀蛋白质的纯化,例如我们的Anti-Myc 磁珠和Anti-DYKDDDDK(FLAG)磁珠(已终卖)。
     
    ■ 应用
    免疫沉淀
     
     
    产品详情请点击:磁珠法免疫沉淀 Buffer Set
     
     

    页面更新:2019-11-12 10:16:45

    Western BLoT Blocking Buffer(Fish Gelatin)酶试剂盒Takara


    Western BLoT Blocking Buffer(Fish Gelatin)
    品牌 Code No. 产品名称 包装量 价格(元) 说明书 数量
    Takara T7131A Western BLoT Blocking Buffer (Fish Gelatin) 1 L ¥1,382
    ¥1,106
    Western BLoT Blocking Buffer(Fish Gelatin) Western BLoT Blocking Buffer(Fish Gelatin) Western BLoT Blocking Buffer(Fish Gelatin)

    *红字为促销价格,促销时间: 2022年9月1日-2022年10月31日

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    ■ 制品内容
    Western BLoT Blocking Buffer (Fish Gelatin) 1 L
     
    ■ 制品说明
    本制品是在TBS-T Buffer中添加了鱼源明胶的封闭缓冲液。由于封闭缓冲液是鱼源的,同哺乳动物来源的奶粉和BSA相比,不会与哺乳动物来源抗体出现非特异性交叉反应。使用本制品可抑制背景,能够明确地检测出特异性抗原。*
    本制品是即用型试剂,无需稀释,可直接用于封闭Western印迹膜;也可作为一抗、HRP或AP标记二抗的稀释液。
    *:· 不是所有Western印迹都可以通用一种封闭试剂。需要进行预实验确定实验体系中最适封闭试剂。一般情况下使用鱼明胶作为封闭试剂时,可抑制背景,能够明确地检测出抗原。   
    · 另一方面,因抗原不同,有时抗原被明胶遮蔽,导致检测灵敏度下降。这种情况下使用BSA、酪蛋白等其他封闭试剂可获得较好的检测结果。
    · 另外,需要使用Protein Free的封闭缓冲液时,请使用Western BLoT Blocking Buffer (Protein Free)(Code No. T7132A)。


     
    ■ 保存
    4℃
     
    ■ 关联资料
    Western Blotting实验操作指南: 点击图片下载 Western BLoT Blocking Buffer(Fish Gelatin)
     
     

    页面更新:2019-12-28 14:13:02