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biolegend 444107 mouse MIF elisa kit代理

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biolegend 444107 mouse MIF elisa kit代理

  • 产品型号：  
  • 简单描述
  • biolegend 444107 mouse MIF elisa kit代理规格：96T 预包被现货供应
详细介绍

biolegend 444107 mouse MIF elisa kit代理

Kit Contents
 
  • Anti-Mouse MIF Pre-coated 96 well Strip Microplate
  • Mouse MIF Detection Antibody
  • Mouse MIF Standard
  • Avidin-HRP
  • Assay Buffer B
  • Wash Buffer (20X)
  • Substrate Solution F
  • Stop Solution
  • Plate Sealers

biolegend 444107 mouse MIF elisa kit代理

Product Details
 
Application: ELISA
Standard Range: 156 – 10,000 pg/mL
Sensitivity: 25.28 ± 1.68 pg/mL

Description:

Macrophage migration inhibitory factor (MIF), also known as glycosylation inhibiting factor (GIF), is a pituitary hormone and a macrophage- and T cell-derived cytokine that plays an important role in the endocrine and immune systems. MIF consists of 115 amino acids and has a molecular weight of 12.5 kD. Mouse MIF shares most of its identity with human (90%), rat (99%), and cattle (88%) MIFs, and a moderate percentage of its identity with MIFs from a wide variety of other species. It exists in monomeric, dimeric, and trimeric forms of identical protomers.

MIF is active as a pro-inflammatory regulator and upregulates multiple cytokines that are vital to combating infections in vivo, including tumor-necrosis factor (TNF), interferon-γ (IFN-γ), and interleukin-12 (IL-12). It is known to maintain cell survival and promote pro-inflammatory immune responses. MIF also activates the ERK1/ERK2 signaling pathway, which inhibits the immunosuppressive effects of glucocorticoids. MIF also plays a critical role in superantigen-induced toxic shock. It upregulates several factors that enhance the growth and invasiveness of gram-negative bacteria. MIF can enhance many inflammatory diseases, such as acute respiratory distress syndrome, arthritis, atopic dermatitis, allograft rejection, glomerulonephritis, inflammatory bowel diseases, asthma, and atherosclerosis. Known receptors for MIF include CXCR2, CXCR4, CD44, and CD74.

The LEGEND MAX™ Mouse MIF ELISA kit is a ready-to-use Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with a 96-well strip plate that is pre-coated with a rat monoclonal anti-mouse MIF antibody. The Detection Antibody is a biotinylated goat polyclonal anti-mouse MIF antibody. This assay is specifically designed to quantify mouse MIF from serum, plasma, cell culture supernatant, and cell lysate. This kit is analytically validated with prepared reagents.

 

Biolegend Human CD28单抗（细胞治疗级） 货号302914302913

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Biolegend Human CD28单抗（细胞治疗级） 货号302914

  • 产品型号：  302913
  • 简单描述
  • Biolegend Human CD28单抗（细胞治疗级） 货号302914规格：302913 规格：50ug302914 规格：500ug302923 规格：1mg现货供应
详细介绍

Biolegend Human CD28单抗（细胞治疗级） 货号302914

通用名称：T淋巴细胞活化用抗人CD28单抗

Biolegend Human CD28单抗（细胞治疗级） 货号302914

CD28分子存在于大多数T细胞表面，被认为是一种T细胞*的表面分子。T细胞对抗原的反应性主要通过CD3/TCR复合物介导，但也有赖于T细胞表面其他分子的协同。ＣＤ２８与其天然配基Ｂ７结合可发挥共刺激作用，作为第二信号介绍Ｔ细胞激活，促进Ｔ细胞增殖及分泌多种细胞因子并介绍导Ｔ，Ｂ细胞粘附。在体外T淋巴细胞激活扩增中抗人CD28单克隆抗体通过第二信号通路发挥共刺激作用。

简单信息：

Clone: CD28.2 (See other available formats)
Isotype: Mouse IgG1, κ
Isotype Control: LEAF™ Purified Mouse IgG1, κ Isotype Ctrl
Workshop
Number:		 V-CD28.05
Reactivity: Human, Cross-Reactivity: Chimpanzee, Baboon, Cynomolgus, Rhesus, Pigtailed Macaque, Squirrel Monkey, Capuchin Monkey, Sooty Mangabey
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.1 EU/µg of the protein (<0.01 ng/µg of the protein) as determined by the LAL test.
Preparation: The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: 1.0 mg/ml

订购信息：

302913 规格：50ug
302914 规格：500ug
302923 规格：1mg

Biolegend LEAF Purified Human CD28单抗302913302914

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Biolegend LEAF Purified Human CD28单抗302913

  • 产品型号：  302914
  • 简单描述
  • Biolegend LEAF Purified Human CD28单抗302913302913 规格：50ug302914 规格：500ug302923 规格：1mg现货！
详细介绍

Biolegend LEAF Purified Human CD28单抗302913

Clone: CD28.2 (See other available formats)
Isotype: Mouse IgG1, κ
Isotype Control: LEAF™ Purified Mouse IgG1, κ Isotype Ctrl
Workshop
Number:		 V-CD28.05
Reactivity: Human, Cross-Reactivity: Chimpanzee, Baboon, Cynomolgus, Rhesus, Pigtailed Macaque, Squirrel Monkey, Capuchin Monkey, Sooty Mangabey
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.1 EU/µg of the protein (<0.01 ng/µg of the protein) as determined by the LAL test.
Preparation: The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: 1.0 mg/ml

Biolegend LEAF Purified Human CD28单抗302913

Storage & Handling: The CD28 antibody solution should be stored undiluted between 2°C and 8°C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: FC – Quality tested
Costim, IP, IHC – Reported in the literature
Recommended Usage: Each lot of this antibody is quality control tested byimmunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Application
Notes:		 Additional reported applications (for the relevant formats) include: immunoprecipitation, immunohistochemical staining of acetone-fixed frozen tissue sections4, and in vitro T cell costimulation5-8. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The CD28.2 antibody co-stimulates T cell proliferation and cytokine production in the presence of suboptimal amounts of anti-CD3 antibody. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 302914). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 302934) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Application
References:		 1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. 
2. Nunes J, et al. 1993. Biochem. J. 293:835. 
3. Calea-Lauri J, et al. 1999. J. Immunol. 163:62.
4. Tazi A, et al. 1999. J. Immunol. 163:3511. (IHC)
5. Marti F, et al. 2001. J. Immunol. 166:197. (Costim)
6. Jeong SH, et al. 2004. J. Virol. 78:6995. (Costim)
7. Rivollier A, et al. 2004. Blood 104:4029. (Costim)
8. Scharschmidt E, et al. 2004. Mol. Cell Biol. 24:3860. (Costim)
9. Sheng W, et al. 2007.Elsevier 580:6819. PubMed
10. Mitsuhashi M. 2007. Clin Chem.53:148. PubMed
11. Ye Z, et al. 2008. Infect. Immun. 76:2541. PubMed
12. Magatti M, et al. 2008. Stem Cells 26:182. (FA) PubMed
  Publication Library
Biolegend LEAF Purified Human CD28单抗302913302914

Human peripheral blood lymphocytes stained with LEAF™ purified CD28.2, followed by anti-mouse IgGs FITC

Biolegend LEAF Purified Human CD28单抗302913302914

Human peripheral blood mononuclear cells were stained with CFSE on day 0, and then stimulated with (filled histogram) or without (open histogram) immobilized LEAF™ Purified CD3 (clone UCHT1) and LEAF™ purified CD28 (clone CD28.2) for 3 days. On day 4, cells were harvested and stained with CD4 Brilliant Violet 711™. Dot plot (above) was analyzed on live cells. Histogram data (below) was analyzed by gating on CD4 positive cells.

Biolegend LEAF Purified Human CD28单抗302913302914

Compare all formats

 
 
 

Antigen Details
 
Description: CD28 is a 44 kD disulfide-linked homodimeric type I glycoprotein. It is a member of the immunoglobulin superfamily and is also known as T44 or Tp44. CD28 is expressed on most T lineage cells, NK cell subsets, and plasma cells. CD28 binds both CD80 and CD86 using a highly conserved motif MYPPY in the CDR3-like loop. CD28 is considered a major co-stimulatory molecule, inducing T lymphocyte activation and IL-2 synthesis, and preventing cell death. In vitro studies indicate that ligation of CD28 on T cells by CD80 and CD86 on antigen presenting cells provides a costimulatory signal required for T cell activation and proliferation.
Other Names: T44, Tp44
Structure: Ig superfamily, type I transmembrane glycoprotein, homodimer, 44 kD
Distribution: Mature T cells, thymocytes, NK cell subsets, plasma cells, EBV-positive B cells
Function: T cell costimulation
Ligand Receptor: CD80, CD86
Antigen
References:		 1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. 
2. June CH, et al. 1994. Immunol. Today 15:321. 
3. Linskey PS, et al. 1993. Annu. Rev. Immunol. 11:191.
GeneID: 940 Biolegend LEAF Purified Human CD28单抗302913302914
UniProt: View information about CD28 on UniProt.org
Keywords: LEAF™ Purified anti-human CD28, CD28.2, LEAF™, T44, Tp44, Human, Cross-Reactivity: Chimpanzee, Baboon, Cynomolgus, Rhesus, Pigtailed Macaque, Squirrel Monkey, Capuchin Monkey, Sooty Mangabey, Activation, Costimulation, Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Biofunctional Assay, Immunology, Antibodies

订购信息：

302913 规格：50ug
302914 规格：500ug
302923 规格：1mg

现货*！

 

Biolegend小鼠抗人CD3抗体（OKT3）317315

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Biolegend小鼠抗人CD3抗体（OKT3）

  • 产品型号：  317315
  • 简单描述
  • Biolegend小鼠抗人CD3抗体（OKT3）规格：1mgClone: OKT3Concentration: 1.0 mg/ml
详细介绍

Biolegend小鼠抗人CD3抗体（OKT3）

Isotype: Mouse IgG2a, κ


 

Application:

FC – Quality tested
IHC, Activ – Reported in the literature

Reactivity:

Human

 

Biolegend LEAF™ Purified anti-human CD3 Antibody317315

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Biolegend LEAF™ Purified anti-human CD3 Antibody

  • 产品型号：  317315
  • 简单描述
  • Clone:OKT3 (See other available formats)Isotype: Mouse IgG2a, κIsotype Control:LEAF™ Purified Mouse IgG2a, κ Isotype Ctrl
详细介绍

LEAF™ Purified anti-human CD3 Antibody

Product Details
 
 
  All CD3 Reagents
Clone: OKT3 （See other available formats）
Isotype: Mouse IgG2a, κ
Isotype Control: LEAF™ Purified Mouse IgG2a, κ Isotype Ctrl
Reactivity: Human
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.1 EU/µg of the protein （<0.01 ng/µg of the protein） as determined by the LAL test.
Preparation: The LEAF™ （Low Endotoxin, Azide-Free） antibody was purified by affinity chromatography.
Concentration: 1.0 mg/ml
Storage & Handling: The CD3 antibody solution should be stored undiluted at 4°C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Application:

FC – Quality tested
IHC, Activ – Reported in the literature
Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
COA:

Enter Lot#:     Biolegend LEAF&trade; Purified anti-human CD3 Antibody317315
Application
Notes:

Clone OKT3 can block the binding of clones SK7 and UCHT1.8 The OKT3 antibody is able to induce T cell activation. Additional reported applications （for the relevant formats） include: immunohistochemical staining of acetone-fixed frozen sections and activation of T cells. The LEAF™ purified antibody （Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered） is recommended for functional assays （Cat. No. 317304）. For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody （Cat. No. 317326） with a lower endotoxin limit than standard LEAF™ purified antibodies （Endotoxin <0.01 EU/µg）.
Application
References:

1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
2. Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York.
3. Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego.
4. Jeong HY, et al. 2008. J. Leuckocyte Biol. 83:755. PubMed
5. Alter G, et al. 2008. J. Virol. 82:9668. PubMed
6. Manevich-Mendelson E, et al. 2009. Blood 114:2344. PubMed
7. Biggs MJ, et al. 2011. J. R. Soc. Interface. 8:1462. PubMed
8. Li B, et al. 2005. Immunology 116:487.
  Publication Library
Biolegend LEAF&trade; Purified anti-human CD3 Antibody317315

Human peripheral blood lymphocytes stained with LEAF™ purified OKT3, followed by anti-mouse IgG FITC

Compare all formats 
 
 
 

Description:

CD3ε is a 20 kD chain of the CD3/T-cell receptor （TCR） complex which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ （CD247）, and a T-cell receptor （α/β or γ/δ） heterodimer. It is found on all mature T lymphocytes, NK-T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.
Other Names: T3, CD3ε
Structure: Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ （CD247） and TCR （α/β or γ/δ） forms CD3/TCR complex, 20 kD
Distribution: Mature T and NK-T cells, thymocyte differentiation
Function: Antigen recognition, signal transduction, T cell activation
Ligand Receptor: Peptide antigen bound to MHC
Antigen
References:

1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego. 
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329. 
3. Lanier L, et al. 1986. J. Immunol. 137:2501.
GeneID: 916 Biolegend LEAF&trade; Purified anti-human CD3 Antibody317315
Latest Publications: View the latest CD3 articles on HighwirePress.com
UniProt: View information about CD3 on UniProt.org
Keywords: LEAF™ Purified anti-human CD3, OKT3, LEAF™, T3, CD3ε
Related Products
 
Description Clone Applications
LEAF™ Purified Mouse IgG2a, κ Isotype Ctrl MOPC-173 FC, WB, IP, ICFC, ICC, IF, IHC, FA
Cell Staining Buffer   FC, ICC, ICFC
RBC Lysis Buffer （10X）   FC, ICFC

Other Formats
 
Description Clone Applications
Alexa Fluor® 488 anti-human CD3 OKT3 FC
Alexa Fluor® 647 anti-human CD3 OKT3 FC
APC anti-human CD3 OKT3 FC
Biotin anti-human CD3 OKT3 FC
Brilliant Violet 510™ anti-human CD3 OKT3 FC
Brilliant Violet 605™ anti-human CD3 OKT3 FC
Brilliant Violet 650™ anti-human CD3 OKT3 FC
Brilliant Violet 711™ anti-human CD3 OKT3 FC
Brilliant Violet 785™ anti-human CD3 OKT3 FC
FITC anti-human CD3 OKT3 FC
Pacific Blue™ anti-human CD3 OKT3 FC
PE anti-human CD3 OKT3 FC
PE/Cy7 anti-human CD3 OKT3 FC
PerCP/Cy5.5 anti-human CD3 OKT3 FC
Purified anti-human CD3 OKT3 FC, IHC
Ultra-LEAF™ Purified anti-human CD3 OKT3 FC, IHC, Activ