This kit enables the fixation and permeabilization of cells which is necessary for staining intracellular cytokines with fluorochrome-conjugated anti-cytokine antibodies. The kit provides two reagents, fixation/permeabilization solution and BD Perm/Wash™ Buffer. After cell fixation and permeabilization, the BD Perm/Wash™ Buffer is used to wash the cells and to dilute the anti-cytokine antibodies for staining.
BD FACSDiva™ CS&T research beads (CS&T research beads) are designed for use on BD™ flow cytometers running BD FACSDiva™ software (v7.0 or later). The beads allow the software to automatically characterize, track, and report measurements of supported BD digital flow cytometers. CS&T research beads are dyed with fluorochromes which are excited by the cytometer’s lasers.
Taz is a 49-kDa transcriptional co-activator with a PDZ-binding motif and is regulated by binding with 14-3-3 proteins. It plays a key role in differentiation of mesenchymal stem cells into either osteoblasts or adipocytes via interactions with key transcription factors Runx2 and PPAR γ . More recently, Taz was found to be a component of an E3 ubiquitin ligase involved in ubiquitin-dependent substrate proteolysis by mediating its interaction with the F-box protein β -Trcp. Therefore, Taz has dual functions of regulating protein degradation and transcription.
FORMAT
Format
Purified
SUGGESTED COMPANION PRODUCTS
Cat No.
Description
Size
611449
HeLa Cell Lysate RUO
500 µg
554656
Stain Buffer (FBS) RUO
500 mL
554655
Fixation Buffer RUO
100 mL
554723
Perm/Wash Buffer RUO
100 mL
554002
HRP Goat Anti-Mouse Ig RUO
1 mL
RESOURCES & TOOLS
Download TDS
PREPARATION AND STORAGE
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
PRODUCT NOTICES
Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences。。ｃｏｍ/pharmingen/protocols for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Triton is a trademark of the Dow Chemical Company.
This antibody has been developed and certified for the bioimaging application. However, a routine bioimaging test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Regulatory Status
RUO
BD代理APC Mouse IgG2b κ Isotype Control Clone 27-35货号：555745
产品详情：
INTENDED USE
The mouse IgG2b, κ immunoglobulin isotype control monoclonal antibody 27-35 is specific for the hapten dansyl (5-[dimethylamino] naphthalene-1-sulfonyl). This hapten is not expressed on human cells or human cell lines. The 27-35 immunoglobulin was selected as an isotype control following testing which demonstrated low background staining on a variety of mouse and human tissues.
FORMAT
Format
APC
Excitation Source
Red 633 nm
Excitation Max
650 nm
Emission Max
660 nm
Allophycocyanin (APC), is an accessory photosynthetic pigment found in blue-green algae. Its molecular weight is approximay 105 kDa. APC has six phycocyanobilin chromophores per molecule, which makes it a very bright fluorochrome that is highly suitable for flow cytometry applications. Due to nearly identical excitation and emission properties but different spillover characteristics, APC and Alexa Fluor® 647 cannot be used simultaneously
Other Formats →
APC-Cy™7
APC-H7
APC-R700
Alexa Fluor® 488
Alexa Fluor® 647
Alexa Fluor® 700
BB515
BUV395
BUV563
BUV737
BV421
BV510
BV650
BV711
BV786
Biotin
FITC
PE
PE-CF594
PE-Cy™5
RESOURCES & TOOLS
Download TDS
STORAGE AND HANDLING
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
PRODUCT NOTICES
This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences。。ｃｏｍ/pharmingen/protocols for technical protocols.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences。。ｃｏｍ/colors.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.
The SK7 (Leu-4) monoclonal antibody specifically binds to the epsilon chain of the CD3 antigen/T-cell antigen receptor (TCR) complex. This complex is composed of at least six proteins that range in molecular weight from 20 to 30 kDa. The antigen recognized by CD3 antibodies is noncovalently associated with either α/β or γ/δ TCR (70 to 90 kDa). The CD3 antigen is present on 61% to 85% of normal peripheral blood lymphocytes 60% to 85% of thymocytes and on Purkinje cells in cerebellum. The soluble form of this antibody has a mitogenic effect on most peripheral blood T lymphocytes, provided appropriate functional monocytes are present.
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
557851
FORMAT
Format
PE-Cy™7
Excitation Source
Blue 488 nm,Green 532 nm,Yellow/Green 561 nm
Excitation Max
496 nm
Emission Max
785 nm
PE-Cy™7 is a tandem fluorochrome that combines PE and a cyanine dye. PE-Cy7 conjugated reagents are as bright as PE conjugates. PE-Cy7 is particularly sensitive to photo-induced degradation, resulting in loss of fluorescence and changes in fluorescence spillover. Extreme caution must be taken to avoid light exposure and prolonged exposure to paraformaldehyde fixative. Fixed cells should be analyzed within 4 hours of fixation in paraformaldehyde or transferred to a paraformaldehyde-free buffer for overnight storage.
The RPA-T4 monoclonal antibody specifically binds to CD4, a 59 kDa single-chain transmembrane glycoprotein that is expressed on T-helper/inducer cell populations. CD4 is also expressed on thymocytes subsets and at lower levels on monocytes and macrophages. CD4 functions as a co-receptor in MHC class II-restricted antigen-induced T cell activation and as a receptor for human immunodeficiency viruses (HIV). This antibody binds to the D1 domain (CDR1 and CDR3 epitopes) of the CD4 antigen and reacts with approximay 80% of thymocytes and 45% of peripheral blood lymphocytes. RPA-T4 is capable of blocking HIV-1, gp120, and inhibits syncytium formation.
This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development or are reported in the literature.
FORMAT
Format
PE
Excitation Source
Blue 488 nm,Green 532 nm,Yellow/Green 561 nm
Excitation Max
496 nm
Emission Max
578 nm
R-phycoerythrin (PE) is an accessory photosynthetic pigment found in red algae. It exists in vitro as a 240-kDa protein with 23 phycoerythrobilin chromophores per molecule. This makes PE one of the brightest fluorochromes for flow cytometry applications, but its photobleaching properties make it unsuitable for fluorescence microscopy.
Other Formats →
APC
APC-Cy™7
APC-H7
APC-R700
Alexa Fluor® 488
Alexa Fluor® 647
Alexa Fluor® 700
BB515
BUV395
BV421
BV605
Biotin
FITC
NA/LE
PE-CF594
PE-Cy™5
PE-Cy™7
Pacific Blue™
PerCP-Cy™5.5
Purified
SUGGESTED COMPANION PRODUCTS
Cat No.
Description
Size
555749
PE Mouse IgG1, κ Isotype Control RUO
100 Tests
RESOURCES & TOOLS
Spectrum Viewer
Panel Designer
Download TDS
PREPARATION AND STORAGE
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
PRODUCT NOTICES
This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences。。ｃｏｍ/pharmingen/protocols for technical protocols.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences。。ｃｏｍ/colors.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.
BD human CD8抗体 Clone RPA-T8宿主物种：mouse反应物种：human应用：流式现货供应
详细介绍
BD human CD8抗体 Clone RPA-T8
Alternative Name：CD8α; CD8A; CD8 alpha; Leu2; MAL; T8; p32
BD human CD8抗体 Clone RPA-T8
Reactivity
Human (QC Testing) Rhesus, Cynomolgus, Baboon (Tested in Development)
Application
Flow cytometry (Routinely Tested)
Immunogen
Human CD8a
Workshop No.
IV T171; V T-CD08.03; VI 6T-CD8.1, 6T-081
Storage Buffer
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
DESCRIPTION
The RPA-T8 monoclonal antibody specifically binds to CD8 alpha (CD8α) . CD8α is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily. CD8α is expressed by the majority of thymocytes, by subpopulations of αβ T cells and γδ T cells and by some NK cells. Cell surface CD8α is expressed either as a disulfide-linked homodimer (CD8αα) or as a heterodimer (CD8αβ) when disulfide-bonded to a CD8 beta chain (CD8β) . CD8-positive αβ T cells coexpress both CD8αα homodimers and CD8αβ heterodimers whereas some γδ T cells and NK cells express CD8αα homodimers. CD8 plays important roles in T cell activation and selection. The extracellular IgSF domain of CD8 α binds to a non-polymorphic determinant on HLA class I molecules (α3 domain) and enables CD8 to function as a co-receptor with MHC class I-restricted TCR during T cell recognition of antigen. The cytoplasmic domain of CD8α associates with Lck, a Src family protein tyrosine kinase that is involved in intracellular signaling. The RPA-T8 and HIT8a monoclonal antibodies are not cross-blocking. This clone has been reported to react with a subset of peripheral blood lymphocytes, but not monocytes nor granuloyctes, of baboon and both rhesus and cynomolgus macaque monkey. In general, a higher frequency of CD8+ and CD4+CD8+ lymphocytes are observed in non-human primates compared to normal human donors.
FORMAT
Format
FITC
Excitation Source
Blue 488 nm
Excitation Max
494 nm
Emission Max
520 nm
FITC, fluorescein isothiocyanate, is a fluorochrome with a molecular weight of 389 Da. FITC is sensitive to pH changes and photobleaching. Due to nearly identical excitation and emission properties but different spillover characteristics, FITC and Alexa Fluor® 488 cannot be used simultaneously. FITC is relatively dim and should be reserved for highly expressed markers whenever possible.
Other Formats →
APC
APC-Cy™7
APC-R700
Alexa Fluor® 488
Alexa Fluor® 647
Alexa Fluor® 700
BB515
BUV395
BUV496
BUV563
BV421
BV510
BV650
BV711
BV786
Biotin
NA/LE
PE
PE-CF594
PE-Cy™5
SUGGESTED COMPANION PRODUCTS
Cat No.
Description
Size
555748
FITC Mouse IgG1, κ Isotype Control RUO
100 Tests
RESOURCES & TOOLS
Spectrum Viewer
Panel Designer
Download TDS
PREPARATION AND STORAGE
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.
PRODUCT NOTICES
This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
An isotype control should be used at the same concentration as the antibody of interest.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Species testing during development may have been performed with a different format of the same clone. Selected applications have been tested for cross-reactivity.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences。。ｃｏｍ/colors.
Please refer to www.bdbiosciences。。ｃｏｍ/pharmingen/protocols for technical protocols.
Storage Buffer：Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
BD PerCP-Cy5.5 Mouse Anti-Human CD56抗体Clone B159
DESCRIPTION
The B159 monoclonal antibody specifically binds to CD56. CD56 is a heavily glycosylated adhesion protein that is present on a subpopulation of peripheral blood large granular lymphocytes that demonstrate natural killer activity. CD56 is also expressed on a subset of T cells but is not expressed on myeloid cells, erythrocytes or B cells. This antigen is a pan-NK-cell marker. CD56 is virtually identical to an isoform of the neural cell adhesion molecule (NCAM), a structure mediating homotypic and heterotypic cell-cell interactions.
FORMAT
Format
PerCP-Cy™5.5
Excitation Source
Blue 488 nm
Excitation Max
482 nm
Emission Max
695 nm
PerCP-Cy™5.5 is a tandem conjugate that combines PerCP with a cyanine dye. PerCP-Cy5.5 is not subject to photobeaching like PerCP and can be used with stream-in-air flow cytometers. It has less Fc receptor–mediated nonspecific staining than PE-Cy5. Additionally, the PerCP-Cy5.5 tandem conjugate is not as susceptible to fixative or light instability compared to APC-Cy7 and PE-Cy7.
Other Formats →
APC
Alexa Fluor® 488
Alexa Fluor® 647
Alexa Fluor® 700
BB515
Biotin
FITC
NA/LE
PE
PE-CF594
PE-Cy™5
PE-Cy™7
Purified
V450
SUGGESTED COMPANION PRODUCTS
Cat No.
Description
Size
550795
PerCP-Cy™5.5 Mouse IgG1 κ Isotype Control RUO
0.1 mg
555899
Lysing Buffer RUO
100 mL
RESOURCES & TOOLS
Spectrum Viewer
Panel Designer
Download TDS
PREPARATION AND STORAGE
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with PerCP-Cy5.5 under optimum conditions, and unconjugated antibody and free PerCP-Cy5.5 were removed. Storage of PerCP-Cy5.5 conjugates in unoptimized diluent is not recommended and may result in loss of signal intensity.
BD human CD16 PE-CY7 MAB抗体宿主：mouse反应物种：human规格：100T应用：流逝
详细介绍
BD human CD16 PE-CY7 MAB抗体
英文名：PE-Cy™7 Mouse Anti-Human CD16
克隆号：Clone 3G8 (RUO)
BD human CD16 PE-CY7 MAB抗体
介绍：
Brand
BD Pharmingen™
Vol. Per Test
5 µl
Isotype
Mouse BALB/c x DBA/2, also known as CD2F1 or CDF1 IgG1, κ
Reactivity
Human (QC Testing) Rhesus, Cynomolgus, Baboon (Reported)
Application
Flow cytometry (Routinely Tested)
Immunogen
Human polymorphonuclear leukocytes
Workshop No.
IV N409
Entrez Gene ID
2214 2215
Storage Buffer
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Regulatory Status
RUO
规格
100 Tests 25 Tests
货号
557744
Product Details
References
DESCRIPTION
The 3G8 monoclonal antibody specifically binds to the 50-65 kDa transmembrane form of the IgG Fc Receptor (FcγRIII), a human NK cell-associated antigen. CD16 is expressed on NK cells as well as macrophages and granulocytes. Reports indicate that CD16 plays a role in signal transduction and NK cell activation. The 3G8 antibody blocks the binding of soluble immune complexes to granulocytes. The 3G8 antibody is reported (Vossebeld et al., 1997) to increase intracellular calcium levels in human neutrophils by interacting with bothFcγRIIa and FcγRIIIb molecules. This antibody has also been reported to induce homotypic neutrophil aggregation.
FORMAT
Format
PE-Cy™7
Excitation Source
Blue 488 nm,Green 532 nm,Yellow/Green 561 nm
Excitation Max
496 nm
Emission Max
785 nm
PE-Cy™7 is a tandem fluorochrome that combines PE and a cyanine dye. PE-Cy7 conjugated reagents are as bright as PE conjugates. PE-Cy7 is particularly sensitive to photo-induced degradation, resulting in loss of fluorescence and changes in fluorescence spillover. Extreme caution must be taken to avoid light exposure and prolonged exposure to paraformaldehyde fixative. Fixed cells should be analyzed within 4 hours of fixation in paraformaldehyde or transferred to a paraformaldehyde-free buffer for overnight storage.
Other Formats →
APC
APC-Cy™7
APC-H7
Alexa Fluor® 647
Alexa Fluor® 700
BUV395
BUV496
BUV737
BV421
BV510
BV605
BV650
BV711
BV786
Biotin
FITC
NA/LE
PE
PE-CF594
PE-Cy™5
SUGGESTED COMPANION PRODUCTS
Cat No.
Description
Size
557872
PE-Cy™7 Mouse IgG1 κ Isotype Control RUO
100 Tests
RESOURCES & TOOLS
Spectrum Viewer
Panel Designer
Download TDS
PREPARATION AND STORAGE
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with PE-Cy7 under optimum conditions, and unconjugated antibody and free PE-Cy7 were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
