标签归档：anti

DAKO F0117兔抗人Rabbit Anti-Human Albumin/FITCF011702-2

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DAKO F0117兔抗人Rabbit Anti-Human Albumin/FITC

  • 产品型号：  F011702-2
  • 简单描述
  • DAKO F0117兔抗人Rabbit Anti-Human Albumin/FITC简介：Code F0117 | Polyclonal Rabbit Anti-Human Albumin/FITC, FITC, Immunofluorescence, 2 mL供应商：agilnet库存状态：现货
详细介绍

DAKO F0117兔抗人Rabbit Anti-Human Albumin/FITC

Polyclonal Rabbit 
Anti-Human 
Albumin/FITC
Code No./ Code/ Code-Nr. F 0117
Edition/ Edition/ Ausgabe 01.08.03
Intended use For in vitro diagnostic use.
F 0117, FITC-Conjugated Polyclonal Rabbit Anti-Human Albumin is intended for the demonstration of albumin in tissue 
sections, and may also be used for other immunofluorescence techniques. Interpretation of results must be made 
within the context of the patient’s clinical history and other diagnostic tests by a certified professional.

本公司长期代理DAKO公司的产品，在上海总部并备有大量现货，常用产品基本上都有现货，欢迎广大科研老师咨询，谢谢。

DAKO抗体
    丹麦的著名抗体生产商，提供世界上质量zui稳定，zui为经典的抗体。其主要产品涉及免疫化学、流式细胞，免疫组织化学，原位杂交，微生物检验等。DAKO的不同显色系统，Envision，LSAB和免疫细胞化学的CAS，GenpointTM和用于分子病理学的PNA=ISH试剂盒，和用于ELISA的AMPAKTM/AmpliQ是其在生物化学研究中的革新产品

DAKO丹科anti-human Kappa Light Chains抗体A0191

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DAKO丹科anti-human Kappa Light Chains抗体

  • 产品型号：  A0191
  • 简单描述
  • DAKO丹科anti-human Kappa Light Chains抗体公司名称 : 丹科医疗DAKO产品类型 : 抗体产品名称 : Kappa Light Chains目录 : A0191克隆性 : 多克隆宿主 : 兔
详细介绍

DAKO丹科anti-human Kappa Light Chains抗体

DAKO抗体是丹麦的著名抗体生产商，提供世界上质量zui稳定，zui为经典的抗体。其主要产品涉及免疫化学、流式细胞，免疫组织化学，原位杂交，微生物检验等。DAKO的不同显色系统，Envision，LSAB和免疫细胞化学的CAS，GenpointTM和用于分子病理学的PNA=ISH试剂盒，和用于ELISA的AMPAKTM/AmpliQ是其在生物化学研究中的革新产品 。

DAKO丹科anti-human Kappa Light Chains抗体

Kappa Light Chains (Multipurpose)

 

DAKO丹科anti-human Kappa Light Chains抗体A0191

These reagents have been produced in a manner that ensures a particularly wide specificity for kappa-chains. The specificity is directed against surface as well as hidden determinants and has been ascertained by gel precipitation techniques and immunohistochemistry.

A0191 is excellent for the typing of free and bound monoclonal kappa-chains by immunoelectrophoresis and immunofixation.

现货供应中！

美国BD公司Purified Mouse anti-TAZ抗体Clone M2-616560235

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美国BD公司Purified Mouse anti-TAZ抗体Clone M2-616

  • 产品型号：  560235
  • 简单描述
  • 美国BD公司Purified Mouse anti-TAZ抗体Clone M2-616规格：0.1mg货号：560235库存：现货
详细介绍

美国BD公司Purified Mouse anti-TAZ抗体Clone M2-616

美国BD公司Purified Mouse anti-TAZ抗体Clone M2-616

规格

0.1 mg

货号

560235

目录价

¥ 5,543.00

Brand

BD Pharmingen™

Alternative Name

WWTR1

Concentration

0.5 mg/ml

Isotype

Mouse BALB/c IgG2b, κ

Reactivity

Human (QC Testing)  Mouse (Tested in Development) 

Application

Western blot (Routinely Tested) 
Bioimaging (Tested During Development) 

Immunogen

Human TAZ Recombinant Protein

Storage Buffer

Aqueous buffered solution containing ≤0.09% sodium azide.

详细说明：

 

DESCRIPTION

 

Taz is a 49-kDa transcriptional co-activator with a PDZ-binding motif and is regulated by binding with 14-3-3 proteins. It plays a key role in differentiation of mesenchymal stem cells into either osteoblasts or adipocytes via interactions with key transcription factors Runx2 and PPAR γ . More recently, Taz was found to be a component of an E3 ubiquitin ligase involved in ubiquitin-dependent substrate proteolysis by mediating its interaction with the F-box protein β -Trcp. Therefore, Taz has dual functions of regulating protein degradation and transcription.

 

 

 

 

 

FORMAT

Format

Purified

SUGGESTED COMPANION PRODUCTS

Cat No. Description Size  
611449 HeLa Cell Lysate RUO 500 µg

 
554656 Stain Buffer (FBS) RUO 500 mL

 
554655 Fixation Buffer RUO 100 mL

 
554723 Perm/Wash Buffer RUO 100 mL

 
554002 HRP Goat Anti-Mouse Ig RUO 1 mL

 

RESOURCES & TOOLS

  • Download TDS

PREPARATION AND STORAGE

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

PRODUCT NOTICES

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences。。ｃｏｍ/pharmingen/protocols for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Triton is a trademark of the Dow Chemical Company.
  5. This antibody has been developed and certified for the bioimaging application. However, a routine bioimaging test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.

 

VECTOR Labs代理Biotinylated Goat Anti-Rabbit IgG AntBA-1000

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VECTOR Labs代理Biotinylated Goat Anti-Rabbit IgG Ant

  • 产品型号：  BA-1000
  • 简单描述
  • VECTOR Labs代理Biotinylated Goat Anti-Rabbit IgG Antibody规格：1.5mg现货供应
详细介绍

VECTOR Labs代理Biotinylated Goat Anti-Rabbit IgG Antibody

Catalog Number BA-1000
Unit Size 1.5 mg
CE Marked Yes
Country of Manufacture United States
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs
Target Species Rabbit
Conjugate Biotinylated
Host Species Goat
Format Concentrate

VECTOR Labs代理Biotinylated Goat Anti-Rabbit IgG Antibody

Features:

  • Affinity-purified, ultrapure, high affinity antibody 
  • Thoroughly adsorbed against serum and immunoglobulins from potentially interfering species
  • Unless otherwise specified, antibodies recognizes both heavy and light chains (H+L)
  • Biotinylated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody
  • Can be used for tissue and cell staining, ELISAs, and blots
  • Included in the VECTASTAIN® ABC kits.
  • Supplied in solution

Product Citations:

    ibody

    我公司代理vector产品，咨询和选购！

    BD PerCP-Cy5.5 Mouse Anti-Human CD56抗体Clone B159560842

    发表于由

    BD PerCP-Cy5.5 Mouse Anti-Human CD56抗体Clone B159

    • 产品型号：  560842
    • 简单描述
    • BD PerCP-Cy5.5 Mouse Anti-Human CD56抗体Clone B159宿主物种：mouse反应物中：human规格：100T克隆号：B159应用：流逝现货供应中
    详细介绍

    BD PerCP-Cy5.5 Mouse Anti-Human CD56抗体Clone  B159

    Brand：BD Pharmingen

    Alternative Name：NCAM1; NCAM-1; Neural cell adhesion molecule 1; NCAM; NKH1; MSK39

    Application：Flow cytometry (Routinely Tested) 

    Immunogen：Human NK Cells

    Storage Buffer：Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

     

    BD PerCP-Cy5.5 Mouse Anti-Human CD56抗体Clone  B159

     

    DESCRIPTION

     

    The B159 monoclonal antibody specifically binds to CD56. CD56 is a heavily glycosylated adhesion protein that is present on a subpopulation of peripheral blood large granular lymphocytes that demonstrate natural killer activity. CD56 is also expressed on a subset of T cells but is not expressed on myeloid cells, erythrocytes or B cells. This antigen is a pan-NK-cell marker. CD56 is virtually identical to an isoform of the neural cell adhesion molecule (NCAM), a structure mediating homotypic and heterotypic cell-cell interactions.

     

     

     

     

     

     

    FORMAT

    BD PerCP-Cy5.5 Mouse Anti-Human CD56抗体Clone B159560842

    Format

    PerCP-Cy™5.5

    Excitation Source

    Blue 488 nm

    Excitation Max

    482 nm

    Emission Max

    695 nm

    PerCP-Cy™5.5 is a tandem conjugate that combines PerCP with a cyanine dye. PerCP-Cy5.5 is not subject to photobeaching like PerCP and can be used with stream-in-air flow cytometers. It has less Fc receptor–mediated nonspecific staining than PE-Cy5. Additionally, the PerCP-Cy5.5 tandem conjugate is not as susceptible to fixative or light instability compared to APC-Cy7 and PE-Cy7.

    Other Formats →

    • APC
    • Alexa Fluor® 488
    • Alexa Fluor® 647
    • Alexa Fluor® 700
    • BB515
    • Biotin
    • FITC
    • NA/LE
    • PE
    • PE-CF594
    • PE-Cy™5
    • PE-Cy™7
    • Purified
    • V450

      SUGGESTED COMPANION PRODUCTS

      Cat No. Description Size  
      550795 PerCP-Cy™5.5 Mouse IgG1 κ Isotype Control RUO 0.1 mg  
      555899 Lysing Buffer RUO 100 mL  

      RESOURCES & TOOLS

      • Spectrum Viewer
      • Panel Designer
      • Download TDS

      PREPARATION AND STORAGE

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with PerCP-Cy5.5 under optimum conditions, and unconjugated antibody and free PerCP-Cy5.5 were removed. Storage of PerCP-Cy5.5 conjugates in unoptimized diluent is not recommended and may result in loss of signal intensity.

       

      DAKO CD20抗体（Mouse Anti-Human CD20cy）M0755

      发表于由

      DAKO CD20抗体（Mouse Anti-Human CD20cy）

      • 产品型号：  M0755
      • 简单描述
      • DAKO CD20抗体（Mouse Anti-Human CD20cy）规格：0.2ml单克隆小鼠抗人CD20cy抗体*!
      详细介绍

      DAKO CD20抗体(Mouse Anti-Human CD20cy）

      克隆号：L26

      该抗体可与一种非常有用的正常和肿瘤性B细胞的非常有用标记的抗原反应。该抗体能与位于CD20抗原上的一种胞质内表位反应，该抗体可与DAKO抗CD3多克隆抗体相配合用于鉴别T细胞和B细胞。该抗体有有助于鉴别霍奇金淋巴瘤中的霍奇金细胞和Read-sternberg细胞

      DAKO CD20抗体(Mouse Anti-Human CD20cy）

       

      现货供应*！

      DAKO（丹科）CD4抗体（Mouse anti-human CD4）M7310

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      DAKO（丹科）CD4抗体（Mouse anti-human CD4）

      • 产品型号：  M7310
      • 简单描述
      • DAKO（丹科）CD4抗体（Mouse anti-human CD4）规格：0.2ml克隆号：Clone 4B12英文名：Monoclonal Mouse Anti-Human CD4 Clone 4B12现货*！
      详细介绍

      DAKO（丹科）CD4抗体（Mouse anti-human CD4）

      英文名：Monoclonal Mouse Anti-Human CD4 Clone 4B12

      克隆号：Clone 4B12

      特别适用于：冰冻切片和石蜡切片

      DAKO（丹科）CD4抗体（Mouse anti-human CD4）

      CD4是一种在正常胸腺细胞、T辅助细胞、大多数成熟外周T细胞以及抑制性或细胞毒性T细胞亚类中表达的跨膜糖蛋白。未在未成熟的胸腺细胞中发现CD4。该抗体有助于鉴别间变性大细胞淋巴瘤、非特定外周T细胞淋巴瘤和肉芽肿病

      Peripheral T-cell lymphoma. The majority of the neoplastic cells show a strong distinct membranous staining reaction.

      Clinical application for IR649/IS649. For identification of thymocytes, T-helper cells and classification of related tumors such as peripheral T-cell lymphoma (unspecified), anaplastic large cell lymphoma and mycosis fungoides.

       

      DAKO鼠抗人CD8抗体anti-human CD8 clone C8/144BIR623

      发表于由

      DAKO鼠抗人CD8抗体anti-human CD8 clone C8/144B

      • 产品型号：  IR623
      • 简单描述
      • DAKO鼠抗人CD8抗体anti-human CD8 clone C8/144B英文名：Monoclonal Mouse Anti-Human antibody
      详细介绍

      DAKO鼠抗人CD8抗体anti-human CD8 clone C8/144B

      CD8 is a 68 kDa transmembrane glycoprotein expressed as a heterodimer by a majority of thymocytes, and by class I major histocompatibility complex restricted, mature, suppressor/cytotoxic T cells.

      The antibody is a useful aid for classification of cytotoxic/suppressor T-cell lymphomas.

       

      详细产品信息和和选购!

      DAKO Monoclonal Mouse Anti-Human CD45R0抗体M0742

      发表于由

      DAKO Monoclonal Mouse Anti-Human CD45R0抗体

      • 产品型号：  M0742
      • 简单描述
      • DAKO Monoclonal Mouse Anti-Human CD45R0抗体Clone UCHL1规格：1ml
      详细介绍

      DAKO Monoclonal Mouse Anti-Human CD45R0抗体

      CD45 is a transmembrane glycoprotein expressed on most nucleated cells of hematopoetic origin. On human leucocytes, five different isoforms of CD45 have been identified, named ABC, AB, BC, B and 0. This antibody reacts with an epitope unique for CD45R0. The antibody labels most thymocytes, a subpopulation of resting T cells within both CD4 and CD8 subsets, and mature, activated T cells. It is effective on formalin-fixed, paraffin-embedded tissue sections. Results aid in the classification of T-cell neoplasms.

      DAKO Monoclonal Mouse Anti-Human CD45R0抗体

      我公司代理DAKO产品，详细产品信息可和选购！

      DAKO抗体是丹麦的著名抗体生产商，提供世界上质量zui稳定，zui为经典的抗体。其主要产品涉及免疫化学、流式细胞，免疫组织化学，原位杂交，微生物检验等。DAKO的不同显色系统，Envision，LSAB和免疫细胞化学的CAS，GenpointTM和用于分子病理学的PNA=ISH试剂盒，和用于ELISA的AMPAKTM/AmpliQ是其在生物化学研究中的革新产品 。

       

       

      BIOTOOL Anti-DYKDDDDK（Flag）亲和凝胶B23101 B23102

      发表于由

      BIOTOOL Anti-DYKDDDDK（Flag）亲和凝胶

      • 产品型号：  B23101 B23102
      • 简单描述
      • BIOTOOL Anti-DYKDDDDK（Flag）亲和凝胶Biotool Anti-Flag（DYKDDDDK） Affinity Gel 由高质量的鼠源 IgG2b 单克隆抗体与sepharose 4B gel 通过供价偶联制备，本产品具有较高的Flag标签融合蛋白加载容量（至少为1.1mg protein /ml gel），杂蛋白非特异结合少，可用于蛋白质免疫共沉淀和少量蛋白质纯化。
      详细介绍

      BIOTOOL Anti-DYKDDDDK(Flag)亲和凝胶

      产品性质

      抗体克隆号 1E6
      抗体亚型 鼠源IgG2b
      抗体纯化方法 Protein A 纯化制备
      抗体结合量 7.5 克抗体/升gel
      适用范围 蛋白质免疫共沉淀，蛋白质纯化
      推荐使用体积 蛋白质免疫共沉淀: 500μl 细胞裂解液 使用 5μl gel
      融合蛋白结合容量 至少为1.1mg protein/ml gel
      结合特性 甲硫氨酸修饰的N端Flag融合蛋白：Met-FLAG–Protein
      N端Flag 融合蛋白：FLAG–Protein
      C端 Flag 融合蛋白：Protein-FLAG
      运输条件 4°C 冰袋

      BIOTOOL Anti-DYKDDDDK(Flag)亲和凝胶

      操作步骤：

      本推荐方法主要适用于检测可以与 Biotool Anti-Flag Affinity Gel 结合的蛋白。
      1、轻微重悬Biotool Anti-Flag Affinity Gel，转移10 μl混合液（5μl gel）至新的离心管中。
      2、加入500 μl TBS. 轻轻的重悬Anti-Flag Affinity Gel，10000 rpm 离心30s, 弃上清，重复上述步骤 3-4 次。
      3、在上述沉淀中加入500 μl细胞裂解液。
      4、4℃ 缓慢孵育2小时。
      5、10000 rpm 离心 30s，上清液转移到新的离心管中保存以后检测。
      6、上步中得到的沉淀用 0.5 ml TBS 洗 3-4 次，至上清液中 OD280 小于 0.05 为止。
      7、上述所得沉淀中加入 10 μl of 2×蛋白上样缓冲液，煮沸 5 min。
      8、进行SDS-PAGE 蛋白检测。

      BIOTOOL Anti-DYKDDDDK(Flag)亲和凝胶

      订购信息：

      B23101 1ML

      B23102 5ML

      B23103 25ML

      B23104 100ML

       

      DAKO Rabbit Anti-Human IgG A0423A0423

      发表于由

      DAKO Rabbit Anti-Human IgG A0423

      • 产品型号：  A0423
      • 简单描述
      • DAKO Rabbit Anti-Human IgG A0423规格：1ml现货*
      详细介绍

      DAKO Rabbit Anti-Human IgG A0423

      DAKO抗体是丹麦的著名抗体生产商，提供世界上质量zui稳定，zui为经典的抗体。其主要产品涉及免疫化学、流式细胞，免疫组织化学，原位杂交，微生物检验等。DAKO的不同显色系统，Envision，LSAB和免疫细胞化学的CAS，GenpointTM和用于分子病理学的PNA=ISH试剂盒，和用于ELISA的AMPAKTM/AmpliQ是其在生物化学研究中的革新产品 。

      DAKO Rabbit Anti-Human IgG A0423

      详细产品信息可和选购，我公司备有大量dako（丹科）公司现货产品！

      DAKO（丹科）anti-human CD31抗体 Clone JC70AM0823

      发表于由

      DAKO（丹科）anti-human CD31抗体 Clone JC70A

      • 产品型号：  M0823
      • 简单描述
      • DAKO（丹科）anti-human CD31抗体 Clone JC70Acompany name : Dakoproduct type : antibodyproduct name : CD31, Endothelial Cellcatalog : M0823
      详细介绍

      DAKO(丹科）anti-human CD31抗体 Clone JC70A

      clonality :

      monoclonal

      host :

      mouse

      reactivity :

      human

      publications citing this reagent :

      1. human (IHC)

        Dimitrijevic I, Edvinsson M, Chen Q, Malmsjo M, Kimblad P, Edvinsson L. Increased expression of vascular endothelin type B and angiotensin type 1 receptors in patients with ischemic heart disease. BMC Cardiovasc Disord. 2009;9:40 pubmed publisher

      2. Hendrickson A, Troilo D, Djajadi H, Possin D, Springer A. Expression of synaptic and phototransduction markers during photoreceptor development in the marmoset monkey Callithrix jacchus. J Comp Neurol. 2009;512:218-31 pubmed publisher

      3. Hendrickson A, Troilo D, Possin D, Springer A. Development of the neural retina and its vasculature in the marmoset Callithrix jacchus. J Comp Neurol. 2006;497:270-86 pubmed

      more info or order :

      Dako product webpage

      product information

      Clone Number :

      JC70A

      Host :

      mouse

      Product Name :

      CD31, Endothelial Cell

      Code :

      M0823

      reactive species :

      human

      more info or order :

      Dako product webpage

      DAKO(丹科）anti-human CD31抗体 Clone JC70A
      详细产品信息可和选购

      Biolegend LEAF™ Purified anti-human CD3 Antibody317315

      发表于由

      Biolegend LEAF™ Purified anti-human CD3 Antibody

      • 产品型号：  317315
      • 简单描述
      • Clone:OKT3 (See other available formats)Isotype: Mouse IgG2a, κIsotype Control:LEAF™ Purified Mouse IgG2a, κ Isotype Ctrl
      详细介绍

      LEAF™ Purified anti-human CD3 Antibody

      Product Details
       
       
        All CD3 Reagents
      Clone: OKT3 （See other available formats）
      Isotype: Mouse IgG2a, κ
      Isotype Control: LEAF™ Purified Mouse IgG2a, κ Isotype Ctrl
      Reactivity: Human
      Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.1 EU/µg of the protein （<0.01 ng/µg of the protein） as determined by the LAL test.
      Preparation: The LEAF™ （Low Endotoxin, Azide-Free） antibody was purified by affinity chromatography.
      Concentration: 1.0 mg/ml
      Storage & Handling: The CD3 antibody solution should be stored undiluted at 4°C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
      Application:

      FC – Quality tested
      IHC, Activ – Reported in the literature
      Recommended Usage:

      Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
      COA:

      Enter Lot#:     Biolegend LEAF&trade; Purified anti-human CD3 Antibody317315
      Application
      Notes:

      Clone OKT3 can block the binding of clones SK7 and UCHT1.8 The OKT3 antibody is able to induce T cell activation. Additional reported applications （for the relevant formats） include: immunohistochemical staining of acetone-fixed frozen sections and activation of T cells. The LEAF™ purified antibody （Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered） is recommended for functional assays （Cat. No. 317304）. For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody （Cat. No. 317326） with a lower endotoxin limit than standard LEAF™ purified antibodies （Endotoxin <0.01 EU/µg）.
      Application
      References:

      1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
      2. Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York.
      3. Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego.
      4. Jeong HY, et al. 2008. J. Leuckocyte Biol. 83:755. PubMed
      5. Alter G, et al. 2008. J. Virol. 82:9668. PubMed
      6. Manevich-Mendelson E, et al. 2009. Blood 114:2344. PubMed
      7. Biggs MJ, et al. 2011. J. R. Soc. Interface. 8:1462. PubMed
      8. Li B, et al. 2005. Immunology 116:487.
        Publication Library
      		Biolegend LEAF&trade; Purified anti-human CD3 Antibody317315

      Human peripheral blood lymphocytes stained with LEAF™ purified OKT3, followed by anti-mouse IgG FITC

      Compare all formats 
       
       
       

      Description:

      CD3ε is a 20 kD chain of the CD3/T-cell receptor （TCR） complex which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ （CD247）, and a T-cell receptor （α/β or γ/δ） heterodimer. It is found on all mature T lymphocytes, NK-T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.
      Other Names: T3, CD3ε
      Structure: Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ （CD247） and TCR （α/β or γ/δ） forms CD3/TCR complex, 20 kD
      Distribution: Mature T and NK-T cells, thymocyte differentiation
      Function: Antigen recognition, signal transduction, T cell activation
      Ligand Receptor: Peptide antigen bound to MHC
      Antigen
      References:

      1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego. 
      2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329. 
      3. Lanier L, et al. 1986. J. Immunol. 137:2501.
      GeneID: 916 Biolegend LEAF&trade; Purified anti-human CD3 Antibody317315
      Latest Publications: View the latest CD3 articles on HighwirePress.com
      UniProt: View information about CD3 on UniProt.org
      Keywords: LEAF™ Purified anti-human CD3, OKT3, LEAF™, T3, CD3ε
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      DNAダメージ検出抗体 Anti-Nitroguanosine polyclonal antibody 同仁化学研究所

      发表于由

      上海金畔生物科技有限公司代理日本同仁化学试剂盒全线产品，欢迎访问日本同仁化学dojindo官网了解更多信息。

      DNAダメージ検出抗体 Anti-Nitroguanosine polyclonal antibody 同仁化学研究所Anti-Nitroguanosine polyclonal antibody

      02 酸化ストレス関連試薬

      Anti-Nitroguanosine polyclonal antibody

      • 酸化ストレス関連試薬
      • 酸化ストレス関連試薬

      DNAダメージ検出抗体

      • 製品コード
        AB01　 Anti-Nitroguanosine polyclonal antibody
      • 化学名
        Polyclonal anti-nitroguanosine antibody
      容　量 メーカー希望
      小売価格      		 富士フイルム
      和光純薬
      50 μg ￥66,200 348-90681
      試験成績書

      SDSダウンロード

      • ご購入方法
      • お問い合わせ

      マニュアル

      • プロトコル DNAダメージ検出抗体 Anti-Nitroguanosine polyclonal antibody 同仁化学研究所 ニトログアノシンを検出したい
        DNAダメージ検出抗体 Anti-Nitroguanosine polyclonal antibody 同仁化学研究所

      技術情報

      検出例

      DNAダメージ検出抗体 Anti-Nitroguanosine polyclonal antibody 同仁化学研究所  DNAダメージ検出抗体 Anti-Nitroguanosine polyclonal antibody 同仁化学研究所

      インフルエンザウイルス感染マウスの肺上皮組織切片と蛍光（Vector Red)標識抗マウス抗体によって
      免疫組織染色した画像。（熊本大学医学部微生物学教室 赤池孝章教授ご提供）

      下図はプレートに固相化したNO2-Guanosine結合BSAと各種の物質に対する、本抗体の反応を競合法ELISAで検定したものである。本抗体が反応する物質は濃度に依存した右下がりの曲線を与え抗体が反応していることを示している。抗体が反応しない物質に対しては、競合物質の濃度に関係なく抗体は固相化したNO2-Guanosine結合BSAに反応するためほぼ一定の値を示す。

      ポリクローナル抗体の反応性（IC50）
      ･強く反応する(1 μmol/L)
      8-NO2-guanosine, 8-NO2-guanine
      ･交差反応なし
      guanosine, guanine, 8-OH-guanine, 3- NO2-tyrosine

      使用濃度
      ELISA (5 μg/mL)、免疫組織染色（10 μg/mL)

      動物種
      ウサギ（日本白色種）

      DNAダメージ検出抗体 Anti-Nitroguanosine polyclonal antibody 同仁化学研究所

      参考文献

      参考文献を表示する

      1) T. Akaike, S. Okamoto, T. Sawa, J. Yoshitake, F. Tamura, K. Ichimori, K. Miyazaki, K. Sasamoto and H. Maeda, "8-nitroguanosine formation in viral pneumonia and its implication for pathogenesis", Proc. Natl. Acad. Sci. USA, 2003, 100, 685.
      2) J. Yoshitake, T. Akaike, T. Akuta, F. Tamura, T. Ogura, H. Esumi and H. Maeda, "Nitric oxide as an endogenous mutagen for Sendai virus without antiviral activity", J. Virol., 2004, 78, 8709.
      3) T. Sawa, M. H. Zaki, T. Okamoto, T. Akuta, Y. Tokutomi, S. Kim-Mitsuyama, H. Ihara, A. Kobayashi, M. Yamamoto, S. Fujii, H. Arimoto and T. Akaike, "Protein S-guanylation by the biological signal 8-nitroguanosine 3',5'-cyclic monophosphate", Nat. Chem. Biol., 2007, 3, 727.
      4) M. H. Zaki, S. Fujii, T. Okamoto, S. Islam, S. Khan, K. A. Ahmed, T. Sawa and T. Akaike, "Cytoprotective function of heme oxygenase 1 induced by a nitrated cyclic nucleotide formed during murine salmonellosis", J. Immunol., 2009, 182, 3746.
      5) Y. Terasaki, T. Akuta, M. Terasaki, T. Sawa, T. Mori, T. Okamoto, M. Ozaki, M. Takeya and T. Akaike, "Guanine nitration in idiopathic pulmonary fibrosis and its implication for carcinogenesis", Am. J. Respir. Crit. Care. Med., 2006, 174, 665.
      6) T. Sawa, M. Tatemichi, T. Akaike, A. Barbin and H. Ohshima, "Analysis of urinary 8-nitroguanine, a marker of nitrative nucleic acid damage, by high-performance liquid chromatography-electrochemical detection coupled with immunoaffinity purification: association with cigarette smoking", Free Radic. Biol. Med., 2006, 40, 711.
      7) K. A. Ahmed, T. Sawa and T. Akaike, "Protein cysteine S-guanylation and electrophilic signal transduction by endogeneous nitro-nucleotides", Amino Acids, 2011, 41(1), 123.
      8) T. Sawa, H. Arimoto and T. Akaike, "Regulation of redox signaling involving chemical conjugation of protein thiols by nitric oxide and electrophiles", Bioconjugate Chem., 2010, 21(7), 1121.

      よくある質問

      Q

      ニトログアノシンは酸化ストレスの指標として使用されますが、ニトログアノシンの他に酸化ストレスマーカーとなるものはありますか？

      A

      酸化ストレスマーカーについてまとめた資料を作成しております。
      カスタマーサポートの視点から、各酸化ストレスマーカーの特徴・測定サンプルの前処理方法などを記載しておりますので、ご参照ください。

      下記URLよりダウンロード可能です。

      「はじめての酸化ストレスマーカー測定プロトコル～カスタマーサポートの視点から～」

      Q

      この抗体を用いることで何がわかりますか？

      A

      8-Nitroguanosineは、一酸化窒素（NO）と活性酸素（スーパーオキサイドアニオンラジカル）によって生じる過酸化亜硝酸（パーオキシナイトライト）によってRNAがニトロ化された核酸です。
      生体組織が炎症を起こすと多量のNOが産生され、多くの過酸化亜硝酸が生じて、グアノシン,デオキシグアノシンをニトロ化することが知られております。
      8-Nitroguanosineの局在や量などを調べることにより、8-Nitroguanosine残基の役割や遺伝子傷害、発癌機構の解明に役立つことが期待されます。

      ＊ポリクローナル抗体で染色された場合、次のような確認を行なうことをお勧めします。
      1.標準の8-Nitroguanineと競合させて染色されなくなること
      2.Sodium hydrosulfiteなどの還元剤で試料中のニトログアノシン、ニトログアニンをアミノグアノシン、アミノグアニンに還元して染色されなくなること

      Q

      抗ニトログアノシン抗体はどのくらい保存できますか？

      A

      有効期間は設けておりませんが、希釈前の段階で冷蔵で1年使用できることを確認しております。

      凍結・融解の繰り返しは劣化を早めますので、一旦解凍後は冷蔵にて保管してください。

      また、希釈されますと吸着などもあり、期間の保証は出来ません。

      DNAダメージ検出抗体 Anti-Nitroguanosine polyclonal antibody 同仁化学研究所 DNAダメージ検出抗体 Anti-Nitroguanosine polyclonal antibody 同仁化学研究所

      取扱条件

      規格
      性状： 本品は、無色～淡黄色微濁液体である。
      力値： 試験適合
      取扱条件
      保存条件: 冷凍
      SDSダウンロード
      DNAダメージ検出抗体 Anti-Nitroguanosine polyclonal antibody 同仁化学研究所

      関連製品

      DNAダメージ検出抗体 Anti-Nitroguanosine monoclonal antibody(Clone#NO2G52) 同仁化学研究所

      发表于由

      上海金畔生物科技有限公司代理日本同仁化学试剂盒全线产品，欢迎访问日本同仁化学dojindo官网了解更多信息。

      DNAダメージ検出抗体 Anti-Nitroguanosine monoclonal antibody(Clone#NO2G52) 同仁化学研究所Anti-Nitroguanosine monoclonal antibody(Clone#NO2G52)

      02 酸化ストレス関連試薬

      Anti-Nitroguanosine monoclonal antibody(Clone#NO2G52)

      • 酸化ストレス関連試薬
      • 酸化ストレス関連試薬

      DNAダメージ検出抗体

      • 製品コード
        AB02　 Anti-Nitroguanosine monoclonal antibody(Clone#NO2G52)
      • 化学名
        Monoclonal anti-nitroguanosine antibody
      容　量 メーカー希望
      小売価格      		 富士フイルム
      和光純薬
      50 μg ￥78,300 341-90671
      試験成績書

      SDSダウンロード

      • ご購入方法
      • お問い合わせ

      マニュアル

      • プロトコル DNAダメージ検出抗体 Anti-Nitroguanosine monoclonal antibody(Clone#NO2G52) 同仁化学研究所 ニトログアノシンを検出したい
        DNAダメージ検出抗体 Anti-Nitroguanosine monoclonal antibody(Clone#NO2G52) 同仁化学研究所

      技術情報

      検出例

      DNAダメージ検出抗体 Anti-Nitroguanosine monoclonal antibody(Clone#NO2G52) 同仁化学研究所

      使用濃度
      ELISA(1 μg/mL)、免疫染色組織(10 μg/mL)

      DNAダメージ検出抗体 Anti-Nitroguanosine monoclonal antibody(Clone#NO2G52) 同仁化学研究所

      図1．モノクローナル抗体NO2G52の反応性
      プレートに固相化したNO2-Guanosine結合BSAと各種の物質に対する、本抗体の反応を
      競合法ELISAで検定したものである。本抗体が反応する物質は濃度に依存した右下がりの
      曲線を与え抗体が反応していることを示している。
      モノクローナル抗体は、8位がニトロ化されたものを幅広く認識し、正常なヌクレオシド
      や核酸塩基には反応しない。抜群の特異性と高力価を有している。

                   ＜無刺激＞                    LPS+INF-γ刺激 　　              L-NMMA投与

      DNAダメージ検出抗体 Anti-Nitroguanosine monoclonal antibody(Clone#NO2G52) 同仁化学研究所 DNAダメージ検出抗体 Anti-Nitroguanosine monoclonal antibody(Clone#NO2G52) 同仁化学研究所 DNAダメージ検出抗体 Anti-Nitroguanosine monoclonal antibody(Clone#NO2G52) 同仁化学研究所

      図2. RAW264.7細胞(murine macrophage cell line)における内因性のグアニンのニトロ化
      蛍光標識したAnti-Nitroguanosine monoclonal antibody(Clone#NO2G52)にて検出。
      （データ提供：熊本大学医学部 教授 赤池孝章先生）

      DNAダメージ検出抗体 Anti-Nitroguanosine monoclonal antibody(Clone#NO2G52) 同仁化学研究所

      参考文献

      参考文献を表示する

      1) T. Akaike, S. Okamoto, T. Sawa, J. Yoshitake, F. Tamura, K. Ichimori, K. Miyazaki, K. Sasamoto and H. Maeda, "8-nitroguanosine formation in viral pneumonia and its implication for pathogenesis", Proc. Natl. Acad. Sci. USA, 2003, 100, 685.
      2) J. Yoshitake, T. Akaike, T. Akuta, F. Tamura, T. Ogura, H. Esumi and H. Maeda, "Nitric oxide as an endogenous mutagen for Sendai virus without antiviral activity", J. Virol., 2004, 78, 8709.
      3) T. Sawa, M. H. Zaki, T. Okamoto, T. Akuta, Y. Tokutomi, S. Kim-Mitsuyama, H. Ihara, A. Kobayashi, M. Yamamoto, S. Fujii, H. Arimoto and T. Akaike, "Protein S-guanylation by the biological signal 8-nitroguanosine 3',5'-cyclic monophosphate", Nat. Chem. Biol., 2007, 3, 727.
      4) M. H. Zaki, S. Fujii, T. Okamoto, S. Islam, S. Khan, K. A. Ahmed, T. Sawa and T. Akaike, "Cytoprotective function of heme oxygenase 1 induced by a nitrated cyclic nucleotide formed during murine salmonellosis", J. Immunol., 2009, 182, 3746.
      5) Y. Terasaki, T. Akuta, M. Terasaki, T. Sawa, T. Mori, T. Okamoto, M. Ozaki, M. Takeya and T. Akaike, "Guanine nitration in idiopathic pulmonary fibrosis and its implication for carcinogenesis", Am. J. Respir. Crit. Care. Med., 2006, 174, 665.
      6) T. Sawa, M. Tatemichi, T. Akaike, A. Barbin and H. Ohshima, " Analysis of urinary 8-nitroguanine, a marker of nitrative nucleic acid damage, by high-performance liquid chromatography-electrochemical detection coupled with immunoaffinity purification: association with cigarette smoking", Free Radic. Biol. Med., 2006, 40, 711.
      7) M. Feelisch, "Nitrated cyclic GMP as a new cellular signal", Nat. Chem. Biol., 2007, 3, 687.
      8) K. A. Ahmed, T. Sawa and T. Akaike, "Protein cysteine S-guanylation and electrophilic signal transduction by endogeneous nitro-nucleotides", Amino Acids, 2011, 41(1), 123. 
      9) T. Sawa, H. Arimoto and T. Akaike, "Regulation of redox signaling involving chemical conjugation of protein thiols by nitric oxide and electrophiles", Bioconjugate Chem., 2010, 21(7), 1121

      よくある質問

      Q

      ニトログアノシンは酸化ストレスの指標として使用されますが、 ニトログアノシンの他に酸化ストレスマーカーとなるものはありますか？

      A

      酸化ストレスマーカーについてまとめた資料を作成しております。
      カスタマーサポートの視点から、各酸化ストレスマーカーの特徴・測定サンプルの前処理方法などを記載しておりますので、ご参照ください。

      下記URLよりダウンロード可能です。

      「はじめての酸化ストレスマーカー測定プロトコル～カスタマーサポートの視点から～」

      Q

      この抗体を用いることで何がわかりますか？

      A

      8-Nitroguanosineは、一酸化窒素（NO）と活性酸素（スーパーオキサイドアニオンラジカル）によって生じる過酸化亜硝酸（パーオキシナイトライト）によってRNAがニトロ化された核酸です。
      生体組織が炎症を起こすと多量のNOが産生され、多くの過酸化亜硝酸が生じて、グアノシン,デオキシグアノシンをニトロ化することが知られております。
      8-Nitroguanosineの局在や量などを調べることにより、8-Nitroguanosine残基の役割や遺伝子傷害、発癌機構の解明に役立つことが期待されます。

      ＊ポリクローナル抗体で染色された場合、次のような確認を行なうことをお勧めします。
      1.標準の8-Nitroguanineと競合させて染色されなくなること
      2.Sodium hydrosulfiteなどの還元剤で試料中のニトログアノシン、ニトログアニンをアミノグアノシン、アミノグアニンに還元して染色されなくなること

      Q

      抗ニトログアノシン抗体はどのくらい保存できますか？

      A

      有効期間は設けておりませんが、希釈前の段階で冷蔵で1年
      使用できることを確認しております。

      凍結・融解の繰り返しは劣化を早めますので、一旦解凍後は冷蔵にて保管してください。

      また、希釈されますと吸着などもあり、期間の保証は出来ません。

      DNAダメージ検出抗体 Anti-Nitroguanosine monoclonal antibody(Clone#NO2G52) 同仁化学研究所 DNAダメージ検出抗体 Anti-Nitroguanosine monoclonal antibody(Clone#NO2G52) 同仁化学研究所

      取扱条件

      規格
      性状： 本品は、無色～淡黄色微濁液体である。
      力値： 試験適合
      取扱条件
      保存条件: 冷凍
      SDSダウンロード
      DNAダメージ検出抗体 Anti-Nitroguanosine monoclonal antibody(Clone#NO2G52) 同仁化学研究所

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      CST Anti-GFP （D5.1） XP抗体2956

      发表于由

      CST Anti-GFP （D5.1） XP抗体

      • 产品型号：  2956
      • 简单描述
      • CST Anti-GFP （D5.1） XP抗体Isotype：Rabbit IgGApplications：W IHC-P IF-IC FReactivity：AllSensitivity：Transfected Only
      详细介绍

      CST Anti-GFP （D5.1） XP抗体

      Specificity / Sensitivity

      GFP （D5.1） XP® Rabbit mAb detects GFP-tagged proteins exogenously expressed in cells.

      Source / Purification

      Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of GFP.

      Western Blotting

      CST Anti-GFP （D5.1） XP抗体2956

      Western blot analysis of extracts from HCC827 cells, untransfected or GFP-transfected, using GFP （D5.1） XP® Rabbit mAb.

      IHC-P （paraffin）

      CST Anti-GFP （D5.1） XP抗体2956

      Immunohistochemical analysis of paraffin-embedded HCC827 cells, untransfected （left） or GFP-transfected （right）, using GFP （D5.1） XP® Rabbit mAb.

      Flow Cytometry

      CST Anti-GFP （D5.1） XP抗体2956

      Flow cytometric analysis of HCC827 cells, untransfected （blue） or GFP-transfected （green）, using GFP （D5.1） XP® Rabbit mAb.

       

      IF-IC

      CST Anti-GFP （D5.1） XP抗体2956

      Confocal immunofluorescent analysis of COS-7 cells expressing a GFP-tagged fusion protein （green） using GFP （D5.1） XP® Rabbit mAb （red）. Blue pseudocolor = DRAQ5® #4084 （fluorescent DNA dye）.

      CST Anti-GFP （D5.1） XP抗体

      Background

      Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties.

      The green-fluorescent protein （GFP） functions as a bioluminescence energy transfer acceptor in the jellyfish Aequorea that maximally absorbs light at 395 nm and has an emission spectrum that peaks at 509 nm. GFP has become a very useful tool as a fusion protein that reports gene expression, traces cell lineage and defines subcellular protein localization （1）.

      1. Miyawaki, A. et al. （2003） Curr Opin Chem Biol 7, 557-62.

      Application References

      • Matheny, R.W. and Nindl, B.C. （2011） Endocrinology 152, 1923-34. Applications: Western Blotting

      Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

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      抗泛素抗体Anti-Ubiquitin antibody Ubi-1ab7254

      发表于由

      抗泛素抗体Anti-Ubiquitin antibody Ubi-1

      • 产品型号：  ab7254
      • 简单描述
      • 抗泛素抗体Anti-Ubiquitin antibody [Ubi-1]ELISA, ICC/IF, IHC-Fr, IHC-P, WB，IHC-FoFr，IPReacts with：Human, Mouse, Rat, Chicken, Cow, Dog, Escherichia coli,Horse
      详细介绍

      抗泛素抗体Anti-Ubiquitin antibody [Ubi-1]

      Description Mouse monoclonal [Ubi-1] to Ubiquitin
      Host species Mouse
      Specificity Ubi-1 was initially screened on ELISA of the immunogen, and subsequently tested on sections of Alzheimer brain. It was one of several
      clones which stained neurofibrillary tangles in frozen sectioned material strongly and specifically. Subsequent studies indicated that Ubi-
      1 is relatively insensitive to formalin fixation and so can be used on mildly fixed histological sections of human brain for studies of
      Alzheimer's disease. Ubi-1 also recognizes other ubiquitinated inclusion bodies such as the Lewy bodies of Parkinson's disease and
      the Pick bodies in Pick's disease. This antibody recognizes polyubiquitin chains much more strongly than monoubiquitinated molecules
      or free ubiquitin. Specifically recognizes ubiquitinated cytoplasmic inclusion bodies.
      Tested applications IHC-P, WB, ELISA, ICC/IF, IHC-Fr
      Cross reactivity Reacts with
      Mouse, Chicken, Cow, Human, Arabidopsis thaliana, Caenorhabditis elegans, Fruit fly （Drosophila melanogaster）
      Immunogen Full length native protein （purified） conjugated to KLH by a Glutaraldehyde linker.
      Target
      Relevance Ubiquitin is a highly conserved protein of about 8.5 kDa molecular weight, which has an ATP dependent role in the targeting of proteins
      for proteolytic degradation. To perform this function, the protein to be degraded is first covalently attached to the C terminus of ubiquitin,
      and the ubiquitinated complex is then recognized by a complex of degradative enzymes. Interestingly, ubiquitin also becomes covalently
      bonded to many types of pathological inclusions, which appear to be resistant to normal degradation. Therefore, ubiquitin antibodies
      are very useful for studies of these inclusions. For example, the neurofibrillary tangles and paired helical filaments diagnostic of
      Alzheimer's disease, Lewy bodies seen in Parkinson's disease, and Pick bodies found in Pick's disease are all heavily ubiquitinated
      and can be readily visualized with ubiquitin antibodies.
      Cellular localization Cell Membrane, Cytoplasmic and Nuclear
      Alternative names FLJ25987 antibody
      MGC8385 antibody
      Polyubiquitin B antibody
      RPS 27A antibody
      RPS27A antibody
      UBA 52 antibody
      UBA 80 antibody
      UBA52 antibody
      UBA80 antibody
      UBB antibody
      UBC antibody
      UBCEP 1 antibody
      UBCEP 2 antibody
      UBCEP1 antibody
      UBCEP2 antibody
      Ubiquitin antibody
      ubiquitin B antibody
      Properties
      Form Liquid
      Storage instructions Store at +4°C short term （1-2 weeks）. Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

      抗泛素抗体Anti-Ubiquitin antibody [Ubi-1] 反应性有其他种属的：人，小鼠，大鼠等等