标签归档：ambion

LIFE ambion trizol LS 200ml货号10296-02810296028

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LIFE ambion trizol LS 200ml货号10296-028

  • 产品型号：  10296028
  • 简单描述
  • LIFE ambion trizol LS 200ml货号10296-028规格：200mlTRIzol® LS Reagent 是苯酚和异硫氰酸胍的即用型单相溶液，设计用于从人、动物、植物、酵母、细菌和病毒来源的液体样品中分离总 RNA。 在样品匀浆或裂解过程中，TRIzol® LS Reagent 一方面保持 RNA 的完整性，另一方面破坏细胞并裂解细胞组分。
详细介绍

LIFE ambion trizol LS 200ml货号10296-028

TRIzol® LS 试剂分离的总 RNA 不含蛋白质和 DNA 杂质。 可用于 Northern blot 分析，点印迹杂交，多聚 (A)+ 选择，体外翻译，RNA 酶保护分析，RT-PCR 和分子克隆。 对于 RT-PCR，当一个外显子中存在两个引物时， 建议用扩增级 DNase I（商品目录号 18068-015）处理分离的 RNA。

TRIzol® LS Reagent 的特点：
• 设计用于液体样品，例如血清和病毒制品
• 使用 TRIzol® LS 试剂与液体样品的比值为 3:1
• 有助于多种大分子或小分子 RNA 的分离

LIFE ambion trizol LS 200ml货号10296-028

述

TRIzol® LS Reagent is a complete, ready-to-use reagent optimized for the isolation of high-quality total RNA, or the simultaneous isolation of RNA, DNA, and protein from a variety of liquid samples. This monophasic solution of phenol and guanidine isothiocyanate is designed to isolate separate fractions of RNA, DNA, and proteins from liquid samples of human, animal, plant, yeast, bacterial, and viral origin, typically within one hour.

• Formulated for use with liquid samples such as serum and virus preparations
• Facilitates recovery of RNA, DNA, and protein from a single liquid sample
• Offers excellent lysis capability, even with difficult biological fluids

Reliably Purify RNA from Multiple Sample Sources
TRIzol® LS Reagent is designed for processing a variety of liquid samples of up to 0.25 ml in volume. TRIzol® LS Reagent differs from the standard TRIzol® Reagent in concentration, which permits larger samples to be processed. Just as with the standard TRIzol® Reagent, the integrity of resulting RNA preparations is maintained by the highly effective inhibition of RNase activity during sample homogenization. The simplicity of the TRIzol® LS Reagent method allows simultaneous processing of a large number of samples. The entire procedure can be completed in 1 hour. Total RNA isolated by TRIzol® LS Reagent is free of protein and DNA contamination.

Formulated to Serve Multiple Isolations
TRIzol® LS Reagent allows you to perform sequential precipitation of RNA, DNA, and proteins from a single sample. After homogenizing the sample with TRIzol® LS Reagent, chloroform is added, and the homogenate is allowed to separate into a clear upper aqueous layer (containing RNA), an interface, and a red lower organic layer (containing the DNA and proteins). RNA is precipitated from the aqueous layer with isopropanol. DNA is precipitated from the aqueous/organic interface with ethanol. Protein is precipitated from the phenol-ethanol layer by isopropanol precipitation. The precipitated RNA, DNA, or protein is washed to remove impurities, and then resuspended for use in downstream applications.

Purified Products are Ideal for Use with a Variety of Applications
Isolated RNA can be used in real-time quantitative PCR (qPCR), northern blot analysis, dot blot hybridization, poly-(A)+ selection, In vitro translation, RNase protection assays, and molecular cloning. Isolated DNA can be used in PCR, restriction enzyme digestion, and Southern blots. Isolated protein can be used for western blots, recovery of some enzymatic activity, and someimmunoprecipitation.

 

Ambion原装Trizol Reagent价格，Invitrogen代理商，LIFE代理15596-018

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Ambion原装Trizol Reagent价格，Invitrogen代理商，LIFE代理

  • 产品型号：  15596-018
  • 简单描述
  • Ambion原装Trizol Reagent价格，Invitrogen代理商，LIFE代理Ambion公司以开发RNA研究方面的产品而*。该公司主要提供RNA分离纯化，RNA保护，RACE，RT-PCR，Northen杂交，突变分析等方面的优质产品。RNA研究相关产品:RNA干扰、microRNA分离富集及功能研究、常规RNA操作试剂，如RNA分离、保存以及相关检测等产品。
详细介绍

Ambion原装Trizol Reagent价格，Invitrogen代理商，LIFE代理

描述

TRIzol® 试剂是一种*的即用型试剂，可用于提取高质量的总RNA或者从各种生物样本中同时提取RNA、DNA和蛋白质。这种酚和异硫氰酸胍单相溶液，可用于提取人、动物、植物、酵母或细菌来源的细胞和组织样本中的纯RNA、DNA和蛋白质，只需一个小时即可完成。

• 从一份样品同时提取RNA、DNA和蛋白质

• 即便是难以处理的样本类型，亦具有出色的裂解能力

• 优化的配方和实验方案，适用于组织、细胞、血清、病毒和细菌等多种样品类型

从多种样本体积和来源获得可靠的纯化RNA

TRIzol® 试剂用于处理人、动物、植物或细菌来源的少量组织 (50-100 mg) 和细胞 (5 × 106)，以及大量组织 (≥1 g) 和细胞 (>107)，均可获得的性能，且附带样本纯化实验方案。在TRIzol® 试剂进行样本匀浆化的过程中，它可以破坏细胞，溶解细胞组分，同时高效的抑制RNA酶的活性，从而维持RNA的完整性。TRIzol® 试剂方法十分简单，可同时处理大量样本。整个过程可在一小时内完成。采用TRIzol® 试剂提取的总RNA不会被蛋白质和DNA污染。

适用于多个分子靶点的提取

TRIzol® 试剂使您可以顺序沉淀单一样本中的RNA、DNA和蛋白质。采用TRIzol® 试剂进行样本匀浆化后，加入氯仿，匀浆物可分成透明的上层水相层 (含有RNA)、相界面和红色的下层有机层 (含有DNA 和蛋白质)。然后用异丙醇从水相层中沉淀出RNA。用乙醇从相界面/有机层中沉淀出DNA。利用异丙醇沉淀从酚-乙醇上清液中沉淀出蛋白质。洗涤沉淀的RNA、DNA或蛋白质，去除杂质，重悬浮后供下游应用。

仅供研究使用。不得用于动物或人类的诊断或治疗。

Ambion原装Trizol Reagent价格，Invitrogen代理商，LIFE代理

订购信息：

15596-018 200ml 现货

15596-026 100ml 现货

 

LIFE ambion PureLink RNA Mini Kit（货号12183025）12183018A

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LIFE ambion PureLink RNA Mini Kit（货号12183025）

  • 产品型号：  12183018A
  • 简单描述
  • LIFE ambion PureLink RNA Mini Kit（货号12183025）12183018A 规格50 preps12183025 规格250 preps
详细介绍

LIFE ambion PureLink RNA Mini Kit（货号12183025）

Ambion公司成立于1988年，从1989年开始在美国德克萨斯州的奥斯丁市开始运营。公司在剑桥和日本都设有分支机构。Ambion本身称为RNA公司自有其道理，自成立以来它所有产品都围绕RNA的。在RNAi这方面，他们从2001年开始提供相关产品，是zui早提供RNAi产品公司之一。
目前Ambion公司提供六种RNAi产品，它们分别是：化学合成、利用试剂盒转录获得、利用RNA酶将长片段双链RNA切割获得、利用质粒表达shRNA、利用PCR产物表达shRNA和从病毒载体表达shRNA。除了siRNA外，公司还提供将100个到1000个siRNA导入细胞的转染和电穿孔产品。

美国Ambion是一家闻名 的RNA公司，自2001年起Ambion开始提供RNAi及其相关产品，经过几年努力，今年Ambion被Science评为zui高技术的RNAi技术公司。

LIFE ambion PureLink RNA Mini Kit（货号12183025）

产品简介：

 

描述

The PureLink® RNA Mini Kit provides a simple, reliable, and rapid column-based method for isolating high-quality total RNA from a wide variety of sources without the need for hazardous reagents such as phenol. Obtain high-yield, high-integrity RNA from animal and plant cells and tissues as well as blood, bacteria, yeast, and liquid samples. The PureLink® RNA Mini Kit provides:

• Rapid, non-hazardous RNA purification capabilities
• Ready-to-use RNA for many downstream applications

Rapid and convenient column purification 
The PureLink® RNA Mini Kit provides rapid purification of total RNA from a wide range of cells and tissue types to yield up to 1,000 µg of purified RNA from a single extraction (see table). High-quality total RNA can be obtained from mini- to midi-prep amounts of starting material with no genomic DNA contamination.

Simplified, nontoxic RNA purification
The PureLink® RNA Mini Kit combines nontoxic guanidine-isothiocyanate lysis with the speed, purity, and ease-of-use of silica-membrane purification. The safe and easy procedure can typically be completed in less than 20 minutes without the need for hazardous phenol/chloroform extraction, CsCl centrifugation, LiCl, or alcohol precipitation.

The PureLink RNA Mini Kit is recommended to be used together with the Homogenizer (Cat. No. 12183026) designed to homogenize cell or tissue lysates via centrifugation, prior to nucleic acid purification. The Homogenizer is especially effective for clarifying particulates from plant tissues.

For plant tissues rich in polyphenolics or starch (e.g., pine needles, potato tubers), we recommend using PureLink™ Plant RNA Reagent (Cat. No. 12322012) together with the PureLink RNA Mini Kit.