日本同仁化学Hampton蛋白结晶试剂盒Magnesium acetate tetrahydrate/HR2-561- DOJINDO

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Products > Optimize Reagents > Optimize – Salts > Magnesium acetate tetrahydrate

Magnesium acetate tetrahydrate

Hampton蛋白结晶试剂盒Magnesium acetate tetrahydrate/HR2-561

CAT NO

HR2-561

NAME

1.0 M Magnesium acetate tetrahydrate

DESCRIPTION

100 mL

PRICE

$109.00

支持材料

Hampton蛋白结晶试剂盒Magnesium acetate tetrahydrate/HR2-561 HR2-561 Magnesium acetate tetrahydrate SDS

应用

  • Crystallization grade Magnesium acetate tetrahydrate for formulating screens or for optimization
  • 结晶级醋酸镁四水合物,用于配制屏幕或优化

特征

  • Sterile filtered solution
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
  • 无菌过滤溶液
    在 1+ 型超纯水中配制:25°C 时电阻率为 18.2 兆欧厘米,总有机碳 < 5 ppb,无细菌(<1 细菌 (CFU/ml)),无热原(<0.03 内毒素 (EU/ml)) , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述Magnesium acetate tetrahydrate

Synonyms: Acetic acid magnesium salt
Mg(CH3COO)2 • 4H2O
C4H6MgO4 • 4H2O
Mr 214.46
CAS Number [16674-78-5]
EC Number 205-554-9
Beilstein Registry Number 3730605
Merck 14,5654
RTECS AI5600000
MDL Number MFCD00149214
Purity 98.0 – 102.0%

Measured pH Range: 7.2 – 8.7 at 25°C
Measured Conductivity Range: 33.9 – 37.6 mS/cm at 25°C
Measured Refractive Index Range: 1.35680 – 1.35782 at 20°C

别名:醋酸镁盐
Mg(CH3COO)2 • 4H2O
C4H6MgO4 • 4H2O
214.46 先生
CAS 编号 [16674-78-5]
欧盟编号 205-554-9
贝尔斯坦注册号 3730605
默克 14,5654
RTECS AI5600000
MDL 编号 MFCD00149214
纯度 98.0 – 102.0%

测得的 pH 值范围:25°C 时为 7.2 – 8.7
测得的电导率范围:25°C 时 33.9 – 37.6 mS/cm
测得的折射率范围:20°C 时为 1.35680 – 1.35782

Insoluble Matter ≤0.005%
Heavy Metals (as Pb) ≤5 ppm
Ba ≤0.001%
Ca ≤0.01%
Cl  ≤0.001%
Fe  ≤5 ppm
K   ≤0.005%
Mn ≤0.001%
Na ≤0.005%
SO4 ≤0.005%
Sr ≤0.005%

Magnesium acetate tetrahydrate

日本同仁化学Hampton蛋白结晶试剂盒Potassium acetate/HR2-671- DOJINDO

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Products > Optimize Reagents > Optimize – Salts > Potassium acetate

Potassium acetate

Hampton蛋白结晶试剂盒Potassium acetate/HR2-671

 

CAT NO

HR2-671

NAME

5.0 M Potassium acetate

DESCRIPTION

200 mL

PRICE

$117.00

支持材料

Hampton蛋白结晶试剂盒Potassium acetate/HR2-671 HR2-671 Potassium acetate SDS

应用

  • Crystallization grade Potassium acetate for formulating screens or for optimization
  • 结晶级醋酸钾,用于筛选或优化

特征

  •  Sterile filtered solution
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
  • 无菌过滤溶液
    在 1+ 型超纯水中配制:25°C 时电阻率为 18.2 兆欧厘米,总有机碳 < 5 ppb,无细菌(<1 细菌 (CFU/ml)),无热原(<0.03 内毒素 (EU/ml)) , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述Potassium acetate

Synonyms: K(acac)
C2H3KO2
CH3COOK
Mr 98.14
CAS Number [127-08-2]
EC Number 204-822-2
Merck 14,7605
RTECS AJ3325000
MDL Number MFCD00012458
Beilstein Registry Number 3595449
Purity ≥ 99.0%

Measured pH range: 7.9 – 9.9 at 25°C
Measured Conductivity Range: 132.3 – 151.2 mS/cm at 25°C
Measured Refractive Index Range: 1.38123 – 1.38216 at 20°C

同义词:K(acac)
C2H3KO2
CH3COOK
98.14 先生
CAS 编号 [127-08-2]
欧盟编号 204-822-2
默克 14,7605
RTECS AJ3325000
MDL 编号 MFCD00012458
贝尔斯坦注册号 3595449
纯度≥99.0%

测得的 pH 值范围:25°C 时 7.9 – 9.9
测得的电导率范围:25°C 时为 132.3 – 151.2 mS/cm
测得的折射率范围:20°C 时为 1.38123 – 1.38216

Insoluble Matter ≤0.005%
Ca ≤0.005%
Cl  ≤0.003%
Fe  ≤5 ppm (parts per million)
Mg ≤0.002%
Na ≤0.03%
Pb  ≤5 ppm (parts per million)
PO4 ≤0.001%
SO4 ≤0.002%

Potassium acetate

日本同仁化学Hampton蛋白结晶试剂盒Ammonium acetate/HR2-799/HR2-565- DOJINDO

上海金畔生物科技有限公司代理日本同仁化学 DOJINDO代理商全线产品,欢迎访问官网了解更多信息

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Products > Optimize Reagents > Optimize – Salts > Ammonium acetate

Ammonium acetate

Hampton蛋白结晶试剂盒Ammonium acetate/HR2-799/HR2-565

CAT NO

HR2-565

NAME

1.0 M Ammonium acetate

DESCRIPTION

100 mL

PRICE

$96.00

CAT NO

HR2-799

NAME

8.0 M Ammonium acetate

DESCRIPTION

200 mL

PRICE

$132.00

应用

  • Crystallization grade Ammonium acetate for formulating screens or for optimization
  • 结晶级醋酸铵,用于筛选或优化

特征

  • Sterile filtered solution
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
  • 无菌过滤溶液
    在 1+ 型超纯水中配制:25°C 时电阻率为 18.2 兆欧厘米,总有机碳 < 5 ppb,无细菌(<1 细菌 (CFU/ml)),无热原(<0.03 内毒素 (EU/ml)) , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述Ammonium acetate

Synonyms: None
C2H7NO2
CH3COONH4
Mr 77.08
CAS Number [631-61-8]
EC Number 211-162-9
Beilstein Registry 4186741
Merck 14,495
RTECS AF3675000
MDL Number MFCD00013066
Purity ≥ 99.0%
UN Number UN 3077 9/PG 3

HR2-565 Measured pH range: 6.8 – 7.2 at 25°C
HR2-565 Measured Conductivity Range: 62.0 – 72.9 mS/cm at 25°C
HR2-565 Measured Refractive Index Range: 1.34344 – 1.34417 at 20°C

HR2-799 Measured pH range: 7.6 – 7.9 at 25°C
HR2-799 Measured Conductivity Range: 68.9 – 105.1 mS/cm at 25°C
HR2-799 Measured Refractive Index Range: 1.39674 – 1.40910 at 20°C

同义词:无
C2H7NO2
CH3COONH4
77.08 先生
CAS 编号 [631-61-8]
欧盟编号 211-162-9
贝尔斯坦注册 4186741
默克 14,495
RTECS AF3675000
MDL 编号 MFCD00013066
纯度≥99.0%
联合国编号 UN 3077 9/PG 3

HR2-565 测得的 pH 范围:25°C 时为 6.8 – 7.2
HR2-565 测得的电导率范围:25°C 时为 62.0 – 72.9 mS/cm
HR2-565 测量的折射率范围:20°C 时为 1.34344 – 1.34417

HR2-799 测得的 pH 值范围:25°C 时为 7.6 – 7.9
HR2-799 测得的电导率范围:25°C 时为 68.9 – 105.1 mS/cm
HR2-799 测量的折射率范围:20°C 时为 1.39674 – 1.40910

Al  ≤0.0005%
As ≤0.00001%
Ba ≤0.0005%
Bi  ≤0.0005%
Ca ≤0.001%
Cd ≤0.0005%
Cl  ≤0.0005%
Co ≤0.0005%
Cr  ≤0.0005%
Cu ≤0.0005%
Fe  ≤0.0002%
K   ≤0.005%
Li   ≤0.0005%
Mg ≤0.0005%
Mn ≤0.0005%
Mo ≤0.0005%
Na  ≤0.005%
Ni   ≤0.0005%
NO3 ≤0.001%
Pb   ≤0.0005%
SO4 ≤0.001%
Sr   ≤0.0005%
Zn   ≤0.0005%

Ammonium acetate

支持材料

Hampton蛋白结晶试剂盒Ammonium acetate/HR2-799/HR2-565 HR2-565 Ammonium acetate 1.0 M solution SDSHampton蛋白结晶试剂盒Ammonium acetate/HR2-799/HR2-565 HR2-799 Ammonium acetate 8.0 M solution SDS

日本同仁化学Hampton蛋白结晶试剂盒Calcium acetate hydrate/HR2-567- DOJINDO

上海金畔生物科技有限公司代理日本同仁化学 DOJINDO代理商全线产品,欢迎访问官网了解更多信息

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等,我们将竭诚为您服务,欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。

Products > Optimize Reagents > Optimize – Salts > Calcium acetate hydrate

Calcium acetate hydrate

Hampton蛋白结晶试剂盒Calcium acetate hydrate/HR2-567

CAT NO

HR2-567

NAME

1.0 M Calcium acetate hydrate

DESCRIPTION

100 mL

PRICE

$93.00

支持材料

Hampton蛋白结晶试剂盒Calcium acetate hydrate/HR2-567 HR2-567 Calcium acetate hydrate SDS

应用

  • Crystallization grade Calcium acetate for formulating screens or for optimization
  • 结晶级醋酸钙,用于配制筛选或优化

特征

  • Sterile filtered solution
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
  • 无菌过滤溶液
    在 1+ 型超纯水中配制:25°C 时电阻率为 18.2 兆欧厘米,总有机碳 < 5 ppb,无细菌(<1 细菌 (CFU/ml)),无热原(<0.03 内毒素 (EU/ml)) , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述Calcium acetate hydrate

Synonyms: Calcium diacetate
(CH3COO)2Ca • xH2O
C4H6CaO4 • xH2O
Mr 158.17 + aq
CAS Number [114460-21-8]
EC Number 200-540-9
Beilstein Registry Number 3692527
Merck 14,1646
RTECS AF7525000
MDL Number MFCD00150019
PubChem Substance ID 24852644
Purity ≥ 99.0%

Measured pH Range: 7.6 – 7.9 at 25°C
Measured Conductivity range: 34.1 – 38.1 mS/cm at 25°C
Measured Refractive Index range: 1.35697 – 1.35776 at 20°C

别名:二醋酸钙
(CH3COO)2Ca • xH2O
C4H6CaO4 • xH2O
先生 158.17 + aq
CAS 编号 [114460-21-8]
欧盟编号 200-540-9
贝尔斯坦注册号 3692527
默克 14,1646
RTECS AF7525000
MDL 编号 MFCD00150019
PubChem 物质 ID 24852644
纯度≥99.0%

测得的 pH 值范围:25°C 时为 7.6 – 7.9
测得的电导率范围:25°C 时 34.1 – 38.1 mS/cm
测得的折射率范围:20°C 时为 1.35697 – 1.35776

As ≤0.00001%
Ba ≤0.0002%
Bi  ≤0.0005%
Cd ≤0.0005%
Cl  ≤0.005%
Co ≤0.0005%
Cr ≤0.0005%
Cu ≤0.0005%
Fe ≤0.0005%
K  ≤0.01%
Li  ≤0.0005%
Mg ≤0.1%
Mn ≤0.0005%
Mo ≤0.0005%
Na ≤0.015%
Ni  ≤0.0005%
Pb ≤0.0005%
SO4 ≤0.02%
Sr ≤0.02%
Zn ≤0.0005%

参考Calcium acetate hydrate

1. Structure of the B-DNA Decamer C-C-A-A-C-I-T-T-G-G in Two Different Space Groups: Conformational Flexibility of B-DNA. Lipanov, A; Kopka, ML; Kaczor-Grzeskowiak, M; Quintana, J; Dickerson, RE. Biochemistry 32, 1373- 1389, 1993.

1. B-DNA 十聚体 C-C-A-A-C-I-T-T-G-G 在两个不同空间群中的结构:B-DNA 的构象灵活性。 利帕诺夫,A; 科普卡,毫升; 卡佐尔-格热斯科维亚克,M; 金塔纳,J; 迪克森,RE。 生物化学 32, 1373-1389, 1993。

日本同仁化学Hampton蛋白结晶试剂盒Lithium acetate dihydrate/HR2-669- DOJINDO

上海金畔生物科技有限公司代理日本同仁化学 DOJINDO代理商全线产品,欢迎访问官网了解更多信息

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等,我们将竭诚为您服务,欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。

Products > Optimize Reagents > Optimize – Salts > Lithium acetate dihydrate

Lithium acetate dihydrate

Hampton蛋白结晶试剂盒Lithium acetate dihydrate/HR2-669

CAT NO

HR2-669

NAME

5.0 M Lithium acetate dihydrate

DESCRIPTION

200 mL

PRICE

$128.00

支持材料

Hampton蛋白结晶试剂盒Lithium acetate dihydrate/HR2-669 HR2-669 Lithium acetate dihydrate SDS

应用

  • Crystallization grade Lithium acetate dihydrate for formulating screens or for optimization
  • 结晶级醋酸锂二水合物,用于配制屏幕或优化

特征

  •  Sterile filtered solution
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
  • 无菌过滤溶液
    在 1+ 型超纯水中配制:25°C 时电阻率为 18.2 兆欧厘米,总有机碳 < 5 ppb,无细菌(<1 细菌 (CFU/ml)),无热原(<0.03 内毒素 (EU/ml)) , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述Lithium acetate dihydrate

Synonyms: Acetic acid lithium salt
CH3COOLi • 2H2O
C2H3LiO2 • 2H2O
Mr 102.02
CAS Number 6108-17-4
EC Number 208-914-3
Merck 14,5521
Beilstein Registry 3564320
RTECS AI5450000
MDL Number MFCD00066949
PubChem Substance ID 24896363

Measured pH Range: 7.2 – 9.3 at 25°C
Measured Conductivity Range: 24.7 – 27.8 mS/cm at 25°C
Measured Refractive Index Range: 1.37922 – 1.38124 at 20°C

别名:醋酸锂盐
CH3COOLi • 2H2O
C2H3LiO2 • 2H2O
102.02 先生
CAS 编号 6108-17-4
欧盟编号 208-914-3
默克 14,5521
贝尔斯坦注册 3564320
RTECS AI5450000
MDL 编号 MFCD00066949
PubChem 物质 ID 24896363

测得的 pH 值范围:25°C 时 7.2 – 9.3
测得的电导率范围:25°C 时 24.7 – 27.8 mS/cm
测得的折射率范围:20°C 时为 1.37922 – 1.38124

Al  ≤0.0005%
Ca ≤0.005%
Cl  ≤0.5%
Cu ≤0.0005%
Fe  ≤0.0005%
K   ≤0.005%
Mg ≤0.001%
NH4+ ≤0.05%
Na ≤0.005%
P   ≤0.0005%
Pb ≤0.001%
SO4 ≤0.05%
Zn ≤0.0005%

参考Lithium acetate dihydrate

1. Crystallization and preliminary X-ray crystallographic analysis of rat calcineurin B homologous protein 1. Y. Naoe, K. Arita, H. Hashimoto, H. Kanazawa, M. Sato and T. Shimizu. Acta Cryst. (2005). F61, 612-613.

1. 大鼠 calcineurin B 同源蛋白的结晶和初步 X 射线晶体学分析 1. Y. Naoe、K. Arita、H. Hashimoto、H. Kanazawa、M. Sato 和 T. Shimizu。 晶体学报。 (2005 年)。 F61, 612-613。

日本同仁化学FDA试剂货号:F209 Fluorescein diacetate- DOJINDO

上海金畔生物科技有限公司代理日本同仁化学 DOJINDO代理商全线产品,欢迎访问官网了解更多信息

FDA试剂货号:F209
Fluorescein diacetate
Fluorescein diacetate
商品信息
储存条件:0-5度保存,避光
运输条件:室温
分子式:

C24H16O7

分子量:

416.38

特点:

 

● 激发和发射波长分别为488 nm和530 nm

● 可用于流式细胞仪

SDS下载

选择规格:
1mg

期货

细胞染色

FDA试剂货号:F209 Fluorescein diacetate

FDA试剂货号:F209 Fluorescein diacetate

规格性状
产品概述
荧光特性
参考文献
常见问题Q&A

规格性状

规格

特性:该产物为白色结晶粉末或固体,可溶于二甲基亚砜。

可溶于二甲基亚砜:试验成功

NMR光谱:试验成功

产品概述

FDA可透过细胞膜并作为荧光素积蓄在活细胞内。由于荧光素较BCECF或Calcein的亲水性低,因此荧光素从细胞中渗漏的量也高。FDA也可用于流式细胞仪。荧光素的激发和发射波长分别为488 nm和530 nm。

荧光特性

荧光特性:λex=488 nm, λem=530 nm

参考文献

1) B. Rotman and B. W. Papermaster, “Membrane Properties of Living Mammalian Cells as Studied by Enzymatic Hydrolysis of Fluorogenic Esters”, Proc. Natl. Acad. Sci. USA, 1966, 55, 134.

2) H. R. Hulett, W. A. Bonner, J. Barrett and L. A. Herzenberg, “Cell Sorting: Automated Separation of Mammalian Cells as a Function of Intercellular Fluorescence”, Science, 1969, 166, 747.

3) K. H. Jones and J. A. Senft, “An Improved Method to Determine Cell Viability by Simultaneous Staining with Fluorescein Diacetate-Propidium Iodide”,J.Histochem.Cytochem., 1985, 33, 77.

4) K. McGinnes, G. Chapman, R. Marks and R. Penny, “A Fluorescence NK Assay Using Flow Cytometry”, J. Immunol. Methods, 1986, 86, 7.

5) W. M. J. Vuist, F. V. Buitenen, M. A. De Rie, A. Hekman, P. Ruemke and C. J. M. Melief, “Potentiation by Interleukin 2 of Burkitt’s Lymphoma Therapy with Anti-Pan B (Anti-CD19) Monoclonal Antibodies in a Mouse Xenotransplantation Model”, Cancer Res., 1989, 49, 3783.

6) E. Prosperi, “Intracellular Turnover of Fluorescein Diacetate. Influence of Membrane Ionic Gradients on Fluorescein Efflux”, Histochem.J., 1990, 22, 227.

常见问题Q&A

Q1:如何使用FDA。

 

 

 

 

 

A1:下面给出一个例子。请根据细胞的种类,观察条件讨论细胞的固定和试剂浓度。

【例1】HeLa细胞的形态观察

将1mg的FDA溶解在2ml的DMSO中(储存溶液)

用PBS(-)溶液稀释,设为2μmol/L。(用5ml PBS(-)稀释8μl贮存溶液)

1)用胰蛋白酶-EDTA等回收HaLa细胞,制备细胞悬浊液。

2)离心分离细胞悬浊液(1000rpm, 3min)

3)除去上清,添加PBS(-)(此时细胞数为105 ~ 106cells /mL)

4)通过粘贴等充分分散。

*因为培养液中的血清等有使背景上升的危险重复②~④的操作充分清洗。

5)在1.5 mL微管中添加用4)得到的30μL细胞悬浊液。

6)在此加入15μL的FDA溶液。

7)盖上微管,37℃下孵育15min。

8)在玻璃罩上放上7)的溶液10μL,从上面再盖上一层玻璃,夹入染色的细胞悬浊液。

9)将盖杯置于荧光显微镜下,用490nm滤光器激发,观察发出黄绿色荧光的活细胞。

例2:C57BL/6小鼠脾脏细胞的双重染色,使用FDA和PI。

k.h.j ones, j.a. senft, J.Histochem.Cytochem., 33, 77 (1985).

将5mg的FDA溶解在1ml的丙酮中(储备溶液)

将上述0.04 mL溶液稀释为10ml PBS(-)。

PI将1mg溶解在50ml PBS(-)中。

在0.2 mL D-PBS溶液中混浊的细胞(2×106 cells/mL)溶液中添加0.1 mL的FDA溶液和0.03 mL的PI溶液。

孵化后观察。

【例3】使用HeLa细胞的细胞膜离子梯度对Fluorescein溶出的影响

·E.Prosperi, Histochem. J., 22, 227 (1990).

将5mg的FDA溶解在1ml的丙酮中(储备溶液)

用PBS(-),终浓度2.4μmol/L染色。

 

Q2:细胞染料的激发波长和发射波长是多少。

A2:

FDA试剂货号:F209 Fluorescein diacetate

日本同仁化学Hampton蛋白结晶试剂盒StockOptions Sodium Acetate Buffer Kit- DOJINDO

上海金畔生物科技有限公司代理日本同仁化学 DOJINDO代理商全线产品,欢迎访问官网了解更多信息

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等,我们将竭诚为您服务,欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。

Products > Optimization Screens > StockOptions Buffer Kits > StockOptions Sodium Acetate Buffer Kit

StockOptions Sodium Acetate Buffer Kit

应用

  • Focused, titrated buffer stocks for crystal screening, optimization, & production
  • 用于晶体筛选、优化和生产的集中滴定缓冲液

特征

  • 0.1 unit increments for fine tuning pH
  • pH 3.6 – 5.6
  • 1.0 M concentrated stocks
  • pKa = 4.8

描述

StockOptions Buffer kits are reagent toolboxes for the macromolecular crystal grower. Stock-Options reagent kits offer precisely formulated, high quality crystallization grade reagent stocks in convenient, cost-effective kits. The chemicals utilized in StockOptions kits are the same crystallization grade, ultra-high purity chemicals utilized in the Hampton Research kits. StockOptions reagents are carefully formulated under strict quality standards to ensure reliable performance and lot-to-lot consistency.

Preformulated stocks remove calculation, measurement, and formulation errors. No more second guessing how the reagent was formulated, what specific chemical was used, when it was made, or how to precisely reproduce that reagent when it is gone and more reagent is required for additional setups.

Each HR2-233 contains 21 unique reagents covering the pH range of 3.6 to 5.6 in 0.1 pH increments. Each reagent is supplied in a 10 milliliter volume and is titrated using HCl.

StockOptions 缓冲液试剂盒是大分子晶体生长器的试剂工具箱。 Stock-Options 试剂盒以方便、经济高效的试剂盒形式提供精确配制的高质量结晶级试剂库存。 StockOptions 套件中使用的化学品与 Hampton Research 套件中使用的结晶级超高纯度化学品相同。 StockOptions 试剂按照严格的质量标准精心配制,以确保可靠的性能和批次间的一致性。

预先配制的库存消除了计算、测量和配方错误。 无需再猜测试剂是如何配制的、使用了何种特定化学品、何时制造,或者在试剂消失后如何精确复制该试剂,并且需要更多试剂来进行额外设置。

每个 HR2-233 包含 21 种独特的试剂,涵盖 3.6 至 5.6 的 pH 范围,以 0.1 pH 为增量。 每种试剂的容量为 10 毫升,并使用 HCl 进行滴定。

Sodium acetate trihydrate
Synonym: Acetic acid sodium salt trihydrate
C2H3NaO2·3H2O
CH3COONa·3H2O
Mr 136.08
CAS Number [6131-90-4]
EC Number 204-823-8
Merck 14,8571
Beilstein Registry Number 3732037
RTECS AJ4580000
MDL Number MFCD00071557
PubChem Substance ID 24886015
Purity >99.5%
pKa = 4.8

Assay
Al <0.0005%
As <0.00001%
Bi <0.0005%
Ca <0.001%
Cd <0.0005%
Cl <0.0005%
Co <0.0005%
Cr <0.0005%
Cu <0.0005%
Fe <0.0005%
K <0.005%
Li <0.0005%
Mg <0.0005%
Mn <0.0005%
Mo <0.0005%
Ni <0.0005%
Pb <0.0005%
PO4 <0.0005%
SO4 <0.002%
Sr <0.0005%
Zn <0.0005%

HR2-233 StockOptions Sodium Acetate Buffer Kit 10 mL tube format

Hampton蛋白结晶试剂盒StockOptions Sodium Acetate Buffer Kit
Hampton蛋白结晶试剂盒StockOptions Sodium Acetate Buffer Kit
Hampton蛋白结晶试剂盒StockOptions Sodium Acetate Buffer Kit
Hampton蛋白结晶试剂盒StockOptions Sodium Acetate Buffer Kit

CAT NO

HR2-233

NAME

StockOptions Sodium Acetate Kit

DESCRIPTION

pH 3.6 to 5.6 in 0.1 pH unit increments

PRICE

$265.00

 

相关项目

  • Sodium acetate trihydrate Buffer
  • Individual StockOptions Sodium Acetate Reagents
  • 三水乙酸钠缓冲液
    个人股票期权乙酸钠试剂

参考

1. Microscale Fluorescent Thermal Stability Assay for Membrane Proteins Structure, Volume 16, Issue 3, 11 March 2008, Pages 351-359 Alexander I. Alexandrov, Mauro Mileni, Ellen Y.T. Chien, Michael A. Hanson, Raymond C. Stevens

2. Crystal structures of reduced, oxidized, and mutated human thioredoxins: evidence for a regulatory homodimer. Weichsel, A; Gasdaska, JR; Powis, G; Montfort, WR. Structure 4, 735- 751, 1996.

3. Increasing the size of microcrystals by fine sampling of pH limits. A. McPherson. J. Appl. Cryst. (1995). 28, 362-365.

1. 膜蛋白结构的微尺度荧光热稳定性分析,第 16 卷,第 3 期,2008 年 3 月 11 日,第 351-359 页 Alexander I. Alexandrov、Mauro Mileni、Ellen Y.T. Chien、迈克尔 A. 汉森、雷蒙德 C. 史蒂文斯

2. 还原、氧化和突变的人硫氧还蛋白的晶体结构:调节同源二聚体的证据。 魏克塞尔,一个; 加斯达斯卡,JR; 鲍伊斯,G; 蒙福特,WR。 结构 4, 735-751, 1996。

3. 通过对 pH 限值进行精细采样来增加微晶的尺寸。 A.麦克弗森。 J.应用。 水晶。 (1995 年)。 28, 362-365。

日本同仁化学Hampton蛋白结晶试剂盒Sodium acetate trihydrate/HR2-543/HR2-763- DOJINDO

上海金畔生物科技有限公司代理日本同仁化学 DOJINDO代理商全线产品,欢迎访问官网了解更多信息

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等,我们将竭诚为您服务,欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。

Products > Optimize Reagents > Optimize – Salts > Sodium acetate trihydrate

Sodium acetate trihydrate

Hampton蛋白结晶试剂盒Sodium acetate trihydrate/HR2-543/HR2-763

CAT NO

HR2-543

NAME

3.0 M Sodium acetate trihydrate

DESCRIPTION

200 mL

PRICE

$99.00

CAT NO

HR2-763

NAME

4.0 M Sodium acetate trihydrate pH 7.0

DESCRIPTION

200 mL

PRICE

$129.00

支持材料

Hampton蛋白结晶试剂盒Sodium acetate trihydrate/HR2-543/HR2-763 HR2-543 Sodium acetate trihydrate 3.0 M solution SDSHampton蛋白结晶试剂盒Sodium acetate trihydrate/HR2-543/HR2-763 HR2-763 Sodium acetate trihydrate pH 7.0 4.0 M solution SDS

应用

  • Crystallization grade Sodium acetate trihydrate for formulating screens or for optimization
  • 结晶级三水醋酸钠,用于配制屏幕或优化

特征

  • Sterile filtered solution
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
  • 无菌过滤溶液
    在 1+ 型超纯水中配制:25°C 时电阻率为 18.2 兆欧厘米,总有机碳 < 5 ppb,无细菌(<1 细菌 (CFU/ml)),无热原(<0.03 内毒素 (EU/ml)) , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述Sodium acetate trihydrate

Synonyms: Acetic acid sodium salt trihydrate
C2H3NaO2 • 3H2O
CH3COONa • 3H2O
Mr 136.08
CAS Number [6131-90-4]
EC Number 204-823-8
Beilstein Registry Number 3732037
RTECS AJ4580000
Merck 14,8571
MDL Number MFCD00071557
PubChem Substance ID 24886015
Purity ≥ 99.5%

HR2-543 is formulated from Sodium acetate trihydrate
HR2-543 Measured pH range: 9.0 – 9.7 at 25°C
HR2-543 Measured Conductivity Range: 73.7 – 81.0 mS/cm at 25°C
HR2-543 Measured Refractive Index Range: 1.36393 – 1.36453 at 20°C

HR2-763 is formulated from Sodium acetate trihydrate and titrated to pH 7.0 using HCl
HR2-763 Measured Conductivity Range: 74.3 – 85.3 mS/cm at 25°C
HR2-763 Measured Refractive Index Range: 1.37334 – 1.37475 at 20°C

别名:醋酸钠盐三水合物
C2H3NaO2 • 3H2O
CH3COONa • 3H2O
136.08 先生
CAS 编号 [6131-90-4]
欧盟编号 204-823-8
贝尔斯坦注册号 3732037
RTECS AJ4580000
默克 14,8571
MDL 编号 MFCD00071557
PubChem 物质 ID 24886015
纯度≥99.5%

HR2-543 由三水醋酸钠配制而成
HR2-543 测得的 pH 范围:25°C 时为 9.0 – 9.7
HR2-543 测得的电导率范围:25°C 时 73.7 – 81.0 mS/cm
HR2-543 测量的折射率范围:20°C 时为 1.36393 – 1.36453

HR2-763 由三水乙酸钠配制而成,并使用 HCl 滴定至 pH 7.0
HR2-763 测得的电导率范围:25°C 时 74.3 – 85.3 mS/cm
HR2-763 测量的折射率范围:20°C 时为 1.37334 – 1.37475

Al  ≤0.0005%
As ≤0.00001%
Bi  ≤0.0005%
Ca ≤0.001%
Cd ≤0.0005%
Cl  ≤0.0005%
Co ≤0.0005%
Cr ≤0.0005%
Cu ≤0.0005%
Fe ≤0.0005%
K  ≤0.005%
Li  ≤0.0005%
Mg ≤0.0005%
Mn ≤0.0005%
Mo ≤0.0005%
Ni  ≤0.0005%
Pb ≤0.0005%
PO4 ≤0.0005%
SO4 ≤0.002%
Sr  ≤0.0005%
Zn ≤0.0005%

Sodium acetate trihydrate

Phorbol 12-myristate 13-acetate供应SIGMA代理 P8139

Phorbol 12-myristate 13-acetate供应

  • 产品型号:  SIGMA代理 P8139
  • 简单描述
  • ≥99% (TLC) solubility:DMSO: soluble (Dissolve in water-miscible organic solvent prior to preparing aqueous solutions. DMSO solutions can be stored dark at -20 °C for at least six months.)
详细介绍

别名: 12-O-Tetradecanoylphorbol 13-acetate, 4β,9α,12β,13α,20-Pentahydroxytiglia-1,6-dien-3-one 12-tetradecanoate 13-acetate, PMA, TPA

详细产品信息请咨询

P 物质乙酸盐水合物Substance P acetate salt hydratesigma s6883

P 物质乙酸盐水合物Substance P acetate salt hydrate

  • 产品型号:  sigma s6883
  • 简单描述
  • P 物质乙酸盐水合物Substance P acetate salt hydrate≥95% (HPLC), powder mol wt mol wt 1348 Da by calculation (Average Mass) color white solubility H2O: soluble1 mg/mL
详细介绍

P 物质乙酸盐水合物Substance P acetate salt hydrate

Product Name P 物质 乙酸盐 水合物,
≥95% (HPLC), powder
Product Number S6883
Product Brand SIGMA
CAS Number 137348-11-9 (free base)
Molecular Weight 1347.63 (anhydrous free base basis)
 
TEST SPECIFICATION
Appearance (Color) White
Appearance (Form) Powder
Solubility (Color)

Colorless

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Solubility (Turbidity) Clear
  at 1 mg/ml in 0.1 M HOAc
Water (by Karl Fischer) ≤10 %
% Acetate ≤14.3
% Peptide content ≥75
% Purity (HPLC) ≥95
Note ——————————
 

日本同仁化学Hampton蛋白结晶试剂盒Zinc acetate dihydrate/HR2-563- DOJINDO

上海金畔生物科技有限公司代理日本同仁化学 DOJINDO代理商全线产品,欢迎访问官网了解更多信息

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等,我们将竭诚为您服务,欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。

Products > Optimize Reagents > Optimize – Salts > Zinc acetate dihydrate

Zinc acetate dihydrate

Hampton蛋白结晶试剂盒Zinc acetate dihydrate/HR2-563

CAT NO

HR2-563

NAME

1.0 M Zinc acetate dihydrate

DESCRIPTION

100 mL

PRICE

$102.00

支持材料

Hampton蛋白结晶试剂盒Zinc acetate dihydrate/HR2-563 HR2-563 Zinc acetate dihydrate SDS

应用

  • Crystallization grade Zinc acetate dihydrate for formulating screens or for optimization
  • 结晶级醋酸锌二水合物,用于配制屏幕或优化

特征

  • Sterile filtered solution
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
  • 无菌过滤溶液
    在 1+ 型超纯水中配制:25°C 时电阻率为 18.2 兆欧厘米,总有机碳 < 5 ppb,无细菌(<1 细菌 (CFU/ml)),无热原(<0.03 内毒素 (EU/ml)) , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述Zinc acetate dihydrate

Synonyms: Zinc acetate
Zn(CH3COO)2 • 2H2O
C4H6O4Zn • 2H2O
Mr 219.52
CAS Number [5970-45-6]
EC Number 209-170-2
Beilstein Registry Number 3732513
Merck 14,10128
RTECS ZG8750000
UN Number 3077 9/PG 3
MDL Number MFCD00066961
PubChem Substance ID 24890299
Purity ≥ 99.0%

Measured pH range: 5.7 – 5.8 at 25°C
Measured Conductivity Range: 17.3 – 17.9 mS/cm at 25°C
Measured Refractive Index Range: 1.35728 – 1.35741 at 20°C

别名:醋酸锌
Zn(CH3COO)2 • 2H2O
C4H6O4Zn • 2H2O
219.52 先生
CAS 编号 [5970-45-6]
欧盟编号 209-170-2
贝尔斯坦注册号 3732513
默克 14,10128
RTECS ZG8750000
联合国编号 3077 9/PG 3
MDL 编号 MFCD00066961
PubChem 物质 ID 24890299
纯度≥99.0%

测得的 pH 值范围:25°C 时为 5.7 – 5.8
测得的电导率范围:25°C 时 17.3 – 17.9 mS/cm
测得的折射率范围:20°C 时为 1.35728 – 1.35741

As  ≤0.00001%
Ca ≤0.001%
Cd ≤0.0005%
Cl  ≤0.0005%
Co ≤0.0005%
Cr ≤0.0005%
Cu ≤0.0005%
Fe ≤0.0005%
K   ≤0.005%
Mg ≤0.0005%
Mn ≤0.0005%
N    ≤0.002%
Na  ≤0.005%
Ni   ≤0.0005%
Pb   ≤0.0005%
SO4 ≤0.005%

参考 Zinc acetate dihydrate

1. A new crystal form of carboxypeptidase G from Pseudomonas sp. strain RS-16 which is more amenable to structure determination. Tucker, AD; Rowsell, S; Melton, RG; Pauptit, RA. Acta Crystallogr D 52, 890- 892, 1996.

1. 一种来自假单胞菌属的羧肽酶 G 的新晶型。 应变 RS-16 更适合结构测定。 塔克,广告; 罗塞尔,S; 梅尔顿,RG; 帕普蒂特,RA。 晶体学学报 D 52, 890-892, 1996。

Tris-Acetate-EDTA Buffer (TAE) 50 × Powder, pH8.3酶试剂盒Takara


Tris-Acetate-EDTA Buffer (TAE) 50 × Powder, pH8.3
品牌 Code No. 产品名称 包装量 价格(元) 说明书 数量
Takara T9131 Tris-Acetate-EDTA Buffer (TAE) 50 × Powder, pH8.3 1 pouch ¥2,643 Tris-Acetate-EDTA Buffer (TAE) 50 × Powder, pH8.3 Tris-Acetate-EDTA Buffer (TAE) 50 × Powder, pH8.3 Tris-Acetate-EDTA Buffer (TAE) 50 × Powder, pH8.3
收藏产品 加入购物车
T9131        1 pouch (for 1 L)
■ 规格:
□ 形态: 粉末
□ RNase/DNase activity :
□ 组成(50×): 2.0 M Tris-acetate
0.05 M EDTA
□ 体积: 1,000 ml/pouch
□ pH: 8.3±0.1(25℃)(50×)
 
■ 制品说明
本制品是制备50×Tris-Acetate-EDTA Buffer(TAE)的干粉试剂,用于核酸的琼脂糖或聚丙烯酰胺凝胶电泳。用水溶解本制品,1袋可制备1,000 ml的50×浓度TAE Buffer(pH8.3)。
 
■ 溶解方法
取1袋本制品倒入烧杯或烧瓶中,使用适当量蒸馏水搅拌溶解,使终体积达到1,000 ml。
 
■ 用途
核酸的琼脂糖和聚丙烯酰胺凝胶电泳。
 
■ 保存
室温干燥保存。
 

页面更新:2016-02-16 10:51:24

日本同仁化学Hampton蛋白结晶试剂盒Sodium acetate trihydrate Buffer – 日本同仁化学 DOJINDO代理商日本同仁化学 DOJINDO代理商- DOJINDO

上海金畔生物科技有限公司代理日本同仁化学 DOJINDO代理商全线产品,欢迎访问官网了解更多信息

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等,我们将竭诚为您服务,欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。

Products > Optimize Reagents > Optimize – Buffers > Sodium acetate trihydrate Buffer

Sodium acetate trihydrate Buffer

应用

  • Crystallization grade Sodium acetate trihydrate buffer for formulating screens or for optimization
  • 结晶级三水乙酸钠缓冲液,用于配制筛选或优化

特征

  • Sterile filtered 无菌过滤
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
  • 在 1+ 型超纯水中配制:25°C 时电阻率为 18.2 兆欧厘米,总有机碳 < 5 ppb,无细菌(<1 细菌 (CFU/ml)),无热原(<0.03 内毒素 (EU/ml)) , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述

Formulated from Sodium acetate trihydrate

由醋酸钠三水合物配制而成

Synonyms: Acetic acid sodium salt trihydrate

别名:醋酸钠盐三水合物
C2H3NaO2 • 3H2O
CH3COONa • 3H2O
Mr 136.08
CAS Number [6131-90-4]
EC Number 204-823-8
Merck 14,8571
Beilstein Registry Number 3732037
RTECS AJ4580000
MDL Number MFCD00071557
PubChem Substance ID 24886015
Purity ≥ 99.5%
pKa = 4.8

The useful pH range of a Sodium acetate trihydrate buffer is pH 3.6 – 5.6.

Titrate HR2-569 to the desired pH using HCl
HR2-569 Measured pH Range: 8.7 – 9.3 at 25°C
HR2-569 Measured Conductivity Range: 48.0 – 51.7 mS/cm at 25°C
HR2-569 Measured Refractive Index Range: 1.34395 – 1.34415 at 20°C

HR2-789 has been titrated to pH 4.5 using HCl
HR2-789 Measured Conductivity Range: 60.8 – 67.7 mS/cm at 25°C
HR2-789 Measured Refractive Index Range: 1.34530 – 1.34579 at 20°C

HR2-731 has been titrated to pH 4.6 using HCl
HR2-731 Measured Conductivity Range: 59.3 – 64.5 mS/cm at 25°C
HR2-731 Measured Refractive Index Range: 1.34535 – 1.345745 at 20°C

Al  ≤0.0005%
As ≤0.00001%
Bi  ≤0.0005%
Ca ≤0.001%
Cd ≤0.0005%
Cl  ≤0.0005%
Co ≤0.0005%
Cr  ≤0.0005%
Cu ≤0.0005%
Fe  ≤0.0005%
K   ≤0.005%
Li   ≤0.0005%
Mg ≤0.0005%
Mn ≤0.0005%
Mo ≤0.0005%
Ni  ≤0.0005%
Pb  ≤0.0005%
PO4 ≤0.0005%
SO4 ≤0.002%
Sr  ≤0.0005%
Zn  ≤0.0005%

Sodium acetate trihydrate

Hampton蛋白结晶试剂盒Sodium acetate trihydrate Buffer - 日本同仁化学 DOJINDO代理商日本同仁化学 DOJINDO代理商

CAT NO

HR2-569

NAME

1.0 M Sodium acetate trihydrate

DESCRIPTION

100 mL

PRICE

$41.00

CAT NO

HR2-789

NAME

1.0 M Sodium acetate trihydrate pH 4.5

DESCRIPTION

100 mL

PRICE

$41.00

CAT NO

HR2-731

NAME

1.0 M Sodium acetate trihydrate pH 4.6

DESCRIPTION

100 mL

PRICE

$41.00

相关项目

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参考

1. Crystal structure of thioltransferase at 2.2 A resolution. Katti, SK; Robbins, AH; Yang, Y; Wells, WW. Protein Sci 4, 1998- 2005, 1995.