qiagen质粒大量提取试剂盒12162 12163
- 产品型号：
- 简单描述
- qiagen质粒大量提取试剂盒12162 12163纯化至多10 mg转染级质粒或柯斯质粒DNA纯化得到相当于连续2次CsCl梯度离心得到的DNA纯度高产量质粒DNA经济的制备方法使用LyseBlue，获得的裂解效果和Z大的DNA产量
qiagen质粒大量提取试剂盒12162 12163
QIAGEN Plasmid Kit procedures.
Neutralized bacterial lysates are cleared by centrifugation. The cleared lysate is then loaded onto the anion-exchange tip where plasmid DNA selectively binds under appropriate low-salt and pH conditions. RNA, proteins, metabolites, and other low-molecular-weight impurities are removed by a medium-salt wash, and ultrapure plasmid DNA is eluted in high-salt buffer. The DNA is concentrated and desalted by isopropanol precipitation and collected by centrifugation.
qiagen质粒大量提取试剂盒12162 12163
Performance
QIAGEN Plasmid Kit利用重力流QIAGEN阴离子交换柱技术高效纯化质粒DNA。产量可达10 mg（Giga）、2.5 mg（Mega）、500 µg（Maxi）、或100 µg（Midi）高拷贝质粒DNA（培养体积取决于质粒拷贝数、插入片段大小、宿主菌株和培养基。）QIAGEN Plasmid Kits纯化的质粒DNA适合多种应用
订购信息：
12162质粒大量提取试剂盒QIAGEN Plasmid Maxi Kit (10) 规格：10次
12163质粒大量提取试剂盒QIAGEN Plasmid Maxi Kit (25) 规格：25次
