标签归档：葡聚糖

MP BIO葡聚糖硫酸钠盐DSS型号1601100216011080

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MP BIO葡聚糖硫酸钠盐DSS型号160110

  • 产品型号：  0216011080
  • 简单描述
  • MP BIO葡聚糖硫酸钠盐DSS型号160110用途：提高核酸杂交率 – 溶液中含10%的硫酸葡聚糖，DNA链的再退火率约增加10倍，这一现象进一步扩大了单链或双链的探针与固定在膜上的DNA/RNA的杂交率。不仅如此，添加10%硫酸葡聚糖也许会增加随机切割的双链DNA探针与固定化核酸的杂交率，高达100倍。
详细介绍

MP BIO葡聚糖硫酸钠盐DSS型号160110

应用:

（1）体液免疫的增强和抑制；

（2）B淋巴细胞的多克隆活化作用，甚至可以刺激未成熟的B细胞；

（3）胸腺细胞反应中，促使转化成凝集素；

（4）抑制血液凝固和血小板聚集；

（5）增强血纤维蛋白溶解活性；

（6）增强/抑制细胞介导的免疫应答；

MP BIO葡聚糖硫酸钠盐DSS型号160110

沉淀低密度（LDL）和极低密度（VDLD）脂蛋白 – Mg2+存在的条件下，硫酸葡聚糖沉淀人血清中的低密度脂蛋白，上清液中留下高密度脂蛋白。利用硫酸葡聚糖的沉淀作用去除脂蛋白这一功能同样可以用来纯化其他材料，如β-2-糖蛋白。

作为佐剂发挥作用；αβ

MP BIO葡聚糖硫酸纳盐

溶解度：

溶于水（100mg/ml – 澄清或者轻微模糊的黄色溶液，高分子量的产品在水中的溶解度没有低分子量强）。灭菌前水溶液应配成缓冲体系（如碳酸氢钠），防止降解。 MP Biomedicals  www.mpbio。。ｃｏｍMP Biomedicals生物医学公司是位于美国加利福尼亚州的生命科学和生物技术厂商。2004年底成功收购Qbiogene、ICN，现在有5个大型仓库，能够及时供应各行业需求，亚洲仓库位于新加坡。它致力于生命科学领域的基础研究和探索。目前它提供55000多种产品，是世界上能全面提供生命科学产品和临床诊断产品的*几家厂商之一。

其他相关产品推荐：

MP 216011001 DSS(硫酸钠葡聚糖)(分子量36～50KD)(规格1 GM) MP 216011010 DSS(硫酸钠葡聚糖)(分子量36～50KD)(规格10 GM) MP 216011050 DSS(硫酸钠葡聚糖)(分子量36～50KD)(规格50 GM) MP 216011080 DSS(硫酸钠葡聚糖)(分子量36～50KD)(规格100 GM) MP 216011090 DSS(硫酸钠葡聚糖)(分子量36～50KD)(规格500 GM) MP 215058980  MP 8642852 Freund’s Adjuvant弗氏*佐剂(规格6×10 ml) MP 8642861 Freund’s Adjuvant弗氏不*佐剂(规格50 ml) MP 850494 淋巴细胞分离液Lymphocyte Separation Medium-LSM?(规格5x100ml) MP 850494X 淋巴细胞分离液Lymphocyte Separation Medium-LSM?(规格100ml) Pellet MP 货号 methionine-choline deficient diet(MCD饲料)蛋氨酸/胆碱缺乏饲料(规格10Kg)Powder MP 货号 Choline Deficient Diet with DL-Ethionine无胆碱乙硫氨酸饲料CDE diet(规格10Kg)Powder急性胰腺炎建模 MP 货号 Choline Deficient Diet with DL-Ethionine无胆碱乙硫氨酸饲料CDE diet(规格10Kg)Pellet急性胰腺炎建模 MP 货号 Mineral Mix,AIN-93M(规格2Kg) MP 货号 Mineral Mix,AIN-93M(规格10Kg) MP 货号 Mineral Mix,AIN-93M(规格50Kg) MP 货号 Vitamin Mix,AIN-93(规格1 Kg) MP 货号 Lactalbumin(规格1 lb)   硫酸葡聚糖钠盐 100 g  

MP 93050044 MRA支原体去除试剂Mycoplasma Removal Agent(规格5 ml) MP 91670249 双抗青霉素-链霉素Penicillin-Streptomycin青链霉素混合液(规格100ml) MP 215878291 G418 Sulfate(规格1 G) MP 215878294 G418 Sulfate(规格5 G) MP 2199335 Lipopolysaccharide脂多糖(规格10mg / 100mg / 500mg) MP 215357125 Trypsin胰蛋白酶(规格25 G)

 

 

MP BIO葡聚糖硫酸纳盐0216011080

发表于由

MP BIO葡聚糖硫酸纳盐

  • 产品型号：  0216011080
  • 简单描述
  • MP BIO葡聚糖硫酸纳盐硫酸葡聚糖是葡聚糖的聚阴离子衍生物，由葡聚糖和氯磺酸的酯化反应形成。其中含硫量约为17%，相当于葡聚糖分子的每个葡萄糖残糖中平均含1.9个硫酸基团。
详细介绍

MP BIO葡聚糖硫酸纳盐

重要用途：

·提高核酸杂交率 – 溶液中含10%的硫酸葡聚糖，DNA链的再退火率约增加10倍，这一现象进一步扩大了单链或双链的探针与固定在膜上的DNA/RNA的杂交率。不仅如此，添加10%硫酸葡聚糖也许会增加随机切割的双链DNA探针与固定化核酸的杂交率，高达100倍。

·显示免疫相关的活动：

（1）体液免疫的增强和抑制；

（2）B淋巴细胞的多克隆活化作用，甚至可以刺激未成熟的B细胞；

（3）胸腺细胞反应中，促使转化成凝集素；

（4）抑制血液凝固和血小板聚集；

（5）增强血纤维蛋白溶解活性；

（6）增强/抑制细胞介导的免疫应答；

·沉淀低密度（LDL）和极低密度（VDLD）脂蛋白 – Mg2+存在的条件下，硫酸葡聚糖沉淀人血清中的低密度脂蛋白，上清液中留下高密度脂蛋白。利用硫酸葡聚糖的沉淀作用去除脂蛋白这一功能同样可以用来纯化其他材料，如β-2-糖蛋白。

·作为佐剂发挥作用；αβ

MP BIO葡聚糖硫酸纳盐

溶解度：

溶于水（100mg/ml – 澄清或者轻微模糊的黄色溶液，高分子量的产品在水中的溶解度没有低分子量强）。灭菌前水溶液应配成缓冲体系（如碳酸氢钠），防止降解。

订购信息：

MP BIO葡聚糖硫酸纳盐0216011080MP BIO葡聚糖硫酸纳盐0216011080MP BIO葡聚糖硫酸纳盐0216011080MP BIO葡聚糖硫酸纳盐0216011080

GE amersham SEPHADEX G-50 葡聚糖凝胶G-50填料17-0044-01

发表于由

GE amersham SEPHADEX G-50 葡聚糖凝胶G-50填料

  • 产品型号：  17-0044-01
  • 简单描述
  • GE amersham SEPHADEX G-50 葡聚糖凝胶G-50填料规格：100g现货*
详细介绍

GE amersham SEPHADEX G-50 葡聚糖凝胶G-50填料

简单介绍：

产品：SEPHADEX G-50 COARSE 100G(葡聚糖凝胶G-50)
货号：17-0044-01
包装：100g；分离范围：1000-30000；颗粒大小（微米）：干粉100-300；肽类分离、脱盐、清洗生物提取液、分子量测定
GE Healthcare Life Sciences隶属于通用电气医疗集团，产品和技术主要应用于基因科学、蛋白质科学、药物开发研究、以及生物制药、诊断、法医和环保等行业。

GE amersham SEPHADEX G-50 葡聚糖凝胶G-50填料

Sephadex G-50 Coarse is well established gel filtration medium for desalting and buffer exchange of biomolecules >30 000 molecular weight. The larger bead size of Coarse is suitable for large scale separations .

  • Quickly desalts, removes contaminants and transfers to a new buffer in a single step.
  • Suitable for purification of DNA from small molecules by gel filtration
  • Larger bead size for large scale group separations where high flow rates and low operating pressures are required.
  • Largest bead size in the Sephadex G-50 range.

 

Sephadex is a gel filtration medium prepared by crosslinking dextran with epichlorohydrin. Different types of Sephadex differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex G-50 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex G-50 is available in 4 different particle sizes (Coarse, Medium, Fine & Superfine) and Superfine has the smallest bead size for higher efficiency with shorter diffusion distances. Coarse and Medium are are preferred for large scale group separations where high flow rates and low operating pressures are required.


现货*！

QAE Sephadex葡聚糖凝胶A-25 SP葡聚糖凝胶C-2517-0230-01

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QAE Sephadex葡聚糖凝胶A-25 SP葡聚糖凝胶C-25

  • 产品型号：  17-0230-01
  • 简单描述
  • 离子交换填料GE*QAE Sephadex葡聚糖凝胶A-25 17-0190-01(100G装)SP Sephadex葡聚糖凝胶C-25 17-0230-01(100G装)
详细介绍

QAE Sephadex葡聚糖凝胶A-25 SP葡聚糖凝胶C-25

 

 

产品描述 规格 货号 生产商
产品描述 Sephadex 规格 货号 生产商
Mono P 5/50 GL 1 x 1 ml 17-5170-01 GE Healthcare
Mono P 5/200 GL 1 x 4 ml 17-5171-01 GE Healthcare
PBE 118 200 ml 17-0711-01 GE Healthcare
PBE 94 200 ml 17-0712-01 GE Healthcare
Polybuffer 74 250 ml 17-0713-01 GE Healthcare
Polybuffer 96 250 ml 17-0714-01 GE Healthcare
QAE Sephadex A-25 100 g 17-0190-01 GE Healthcare
QAE Sephadex A-25 500 g 17-0190-02 GE Healthcare
QAE Sephadex A-25 5 kg 17-0190-03 GE Healthcare
QAE Sephadex A-50 100 g 17-0200-01 GE Healthcare
QAE Sephadex A-50 5 kg 17-0200-03 GE Healthcare
SP Sephadex C-25 100 g 17-0230-01 GE Healthcare
SP Sephadex C-25 500 g 17-0230-02 GE Healthcare
SP Sephadex C-25 5 kg 17-0230-03 GE Healthcare
SP Sephadex C-50 100 g 17-0240-01 GE Healthcare
SP Sephadex C-50 500 g 17-0240-02 GE Healthcare
SP Sephadex C-50 5 kg 17-0240-03 GE Healthcare
DEAE Sephadex A-25 100 g 17-0170-01 GE Healthcare
DEAE Sephadex A-25 500 g 17-0170-02 GE Healthcare
DEAE Sephadex A-25 5 kg 17-0170-03 GE Healthcare
DEAE Sephadex A-50 100 g 17-0180-01 GE Healthcare
DEAE Sephadex A-50 500 g 17-0180-02 GE Healthcare
DEAE Sephadex A-50 5 kg 17-0180-03 GE Healthcare
CM Sephadex C-25 100 g 17-0210-01 GE Healthcare
CM Sephadex C-25 500 g 17-0210-02 GE Healthcare
CM Sephadex C-25 5 kg 17-0210-03 GE Healthcare
CM Sephadex C-50 100 g 17-0220-01 GE Healthcare
CM Sephadex C-50 500 g 17-0220-02 GE Healthcare
CM Sephadex C-50 5 kg 17-0220-03 GE Healthcare

GE离子交换填料弱阴离子DEAE葡聚糖凝胶 A-2517-0170-01

发表于由

GE离子交换填料弱阴离子DEAE葡聚糖凝胶 A-25

  • 产品型号：  17-0170-01
  • 简单描述
  • 强阳S、SP、强阴Q、QAE、弱阳CM、弱阴DEAE的区别在于使交换介质守全离子化的PH范围，较宽者为强，较窄者为弱，与结合强度无关。传统的凝胶多数为弱阳CM、弱阴DEAE，同样的纯化工作，可以用强阳S、SP、强阴Q、QAE介质替代。
详细介绍

GE离子交换填料弱阴离子DEAE葡聚糖凝胶 A-25

强阳S、SP、强阴Q、QAE、弱阳CM、弱阴DEAE的区别在于使交换介质守全离子化的PH范围，较宽者为强，较窄者为弱，与结合强度无关。传统的凝胶多数为弱阳CM、弱阴DEAE，同样的纯化工作，可以用强阳S、SP、强阴Q、QAE介质替代。

50较25多孔，孔径较大。对于分子量小于30，000者，25比50的载量高；30,000-100,000者，50则比25的载量高；大于100,000者，结合限于颗粒表面，25的载量更高。

 

 

产品描述 Sephadex 规格 货号 生产商
Mono P 5/50 GL 1 x 1 ml 17-5170-01 GE Healthcare
Mono P 5/200 GL 1 x 4 ml 17-5171-01 GE Healthcare
PBE 118 200 ml 17-0711-01 GE Healthcare
PBE 94 200 ml 17-0712-01 GE Healthcare
Polybuffer 74 250 ml 17-0713-01 GE Healthcare
Polybuffer 96 250 ml 17-0714-01 GE Healthcare
QAE Sephadex A-25 100 g 17-0190-01 GE Healthcare
QAE Sephadex A-25 500 g 17-0190-02 GE Healthcare
QAE Sephadex A-25 5 kg 17-0190-03 GE Healthcare
QAE Sephadex A-50 100 g 17-0200-01 GE Healthcare
QAE Sephadex A-50 5 kg 17-0200-03 GE Healthcare
SP Sephadex C-25 100 g 17-0230-01 GE Healthcare
SP Sephadex C-25 500 g 17-0230-02 GE Healthcare
SP Sephadex C-25 5 kg 17-0230-03 GE Healthcare
SP Sephadex C-50 100 g 17-0240-01 GE Healthcare
SP Sephadex C-50 500 g 17-0240-02 GE Healthcare
SP Sephadex C-50 5 kg 17-0240-03 GE Healthcare
DEAE Sephadex A-25 100 g 17-0170-01 GE Healthcare
DEAE Sephadex A-25 500 g 17-0170-02 GE Healthcare
DEAE Sephadex A-25 5 kg 17-0170-03 GE Healthcare
DEAE Sephadex A-50 100 g 17-0180-01 GE Healthcare
DEAE Sephadex A-50 500 g 17-0180-02 GE Healthcare
DEAE Sephadex A-50 5 kg 17-0180-03 GE Healthcare
CM Sephadex C-25 100 g 17-0210-01 GE Healthcare
CM Sephadex C-25 500 g 17-0210-02 GE Healthcare
CM Sephadex C-25 5 kg 17-0210-03 GE Healthcare
CM Sephadex C-50 100 g 17-0220-01 GE Healthcare
CM Sephadex C-50 500 g 17-0220-02 GE Healthcare
CM Sephadex C-50 5 kg 17-0220-03 GE Healthcare

GE葡聚糖凝胶过滤填料G-25葡聚糖凝胶现货供应 17-0033-0117-0033-02

发表于由

GE葡聚糖凝胶过滤填料G-25葡聚糖凝胶现货供应 17-0033-01

  • 产品型号：  17-0033-02
  • 简单描述
  • 1、Sephadex G-25是脱盐的*――比透析更快、更安全。2、Sephadex G-25Z适合缓冲液置换和去除游离标记，如32P、125I、35S。3、经典的凝胶过滤填料。可提供实验室散装填料和预装柱。所有级别的Sephadex G-25均满足工艺规模的层析需要。
详细介绍

GE葡聚糖凝胶过滤填料G-25葡聚糖凝胶现货供应 17-0033-01

•  Sephadex G-25是脱盐的*――比透析更快、更安全。

 •  Sephadex G-25缓冲液置换和去除游离标记，如32P、125I、35S。

 •  经典的凝胶过滤填料。

可提供实验室散装填料和预装柱。关于高分辨率凝胶过滤，见Superdex填料和层析柱。

所有级别的Sephadex G-25均满足工艺规模的层析需要。

GE葡聚糖凝胶过滤填料G-25葡聚糖凝胶现货供应 17-0033-0117-0033-02

GE葡聚糖凝胶过滤填料G-25葡聚糖凝胶现货供应 17-0033-0117-0033-02

Pharmcia（GE）葡聚糖凝胶G-15Sephadex G-15Pharmcia17-0020-01

发表于由

Pharmcia（GE）葡聚糖凝胶G-15Sephadex G-15

  • 产品型号：  Pharmcia17-0020-01
  • 简单描述
  • Pharmcia（GE)葡聚糖凝胶G-15Sephadex G-15货号：17-0020-02/17-0020-01 品牌：Pharmacia 产品名称：葡聚糖凝胶G-15 17-0020-02/17-0020-01 英文名称：Sephadex G-15 规格：25g/100
详细介绍

Pharmcia（GE)葡聚糖凝胶G-15Sephadex G-15

Sephadex G-15, 100 g

Sephadex G-15 is well established gel filtration medium for desalting and buffer exchange of peptides and small biomolecules >1 500 molecular weight.

  • Quickly desalts, removes contaminants and transfers to a new buffer in a single step.
  • Suitable for buffer exchange and cleanup of biological samples, for example peptides, small proteins, and oligosaccharides larger than 1 500 molecular weight
  • Efficient removal of contaminants such as salts, dyes, and radioactive labels

 

Sephadex is a gel filtration medium prepared by crosslinking dextran with epichlorohydrin. Different types of Sephadex differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex G-15 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex G-50 is available in 4 different particle sizes (Coarse, Medium, Fine & Superfine) and Superfine has the smallest bead size for higher efficiency with shorter diffusion distances. Coarse and Medium are are preferred for large scale group separations at where high flow rates and low operating pressures are required.

 

Pharmcia（GE)葡聚糖凝胶G-15Sephadex G-15

Sephadex G-15, 100 g

Matrix Cross-linked dextran
Particle Size Wet 60 µm-180 µm
Partical Size Dry 40 µm-120 µm
pH Stability Working Range 2-13
Flow Rate < Darcy´s Law1)
Pressure/Flow Specification 20-47 cm/h, pressure drop cm H2O/bed height = 2, bed height 30 cm, 2.6 cm i.d.
Fractionation [Mr] Globular Proteins 1.5 x 103
Fractionation [Mr] Dextrans >< 1.5 x 103
Storage Conditions 4 to 30°C
Autoclavable In wet form (pH 7.0) at 120°C for 30 min
1)The beads behave as rigid spheres obeying Darcy's Law.

Sephadex G-15, 100 g

Handbook
Size Exclusion Chromatography: Principles and Methods Summary

This handbook describes the use of SEC for the purification and separation of biomolecules, with a focus on practical information for obtaining the best results. The media available, selection criteria and examples with detailed instructions for the most common applications are included, as well as the theoretical principles behind the technique. The first step towards a successful separation is to select the correct medium and this handbook focuses on the most up-to-date SEC media and prepacked columns. Close
Magazine
Upstream Downstream issue 2

Close
Material Safety Data Sheet
Sephadex™ G-15, 100 g

Close
Sephadex™ G-15, 100 g

Close
Selection guide
Protein and Peptide Purification: Technique selection guide Summary

This technique selection guide is designed to help you in selecting the technique most suited to your application. It covers ion exchange chromatography (IEX), reversed phase chromatography (RPC), hydrophobic interaction chromatography (HIC), gel filtration (GF), and affinity chromatography (AC). Close

Pharmcia（GE)葡聚糖凝胶G-15Sephadex G-15 详细产品信息可和选购

葡聚糖凝胶G-50Sephadex G-50Pharmcia17-0043-01

发表于由

葡聚糖凝胶G-50Sephadex G-50

  • 产品型号：  Pharmcia17-0043-01
  • 简单描述
  • 葡聚糖凝胶G-50Sephadex G-50肽及球形蛋白质：1500～30000；葡聚糖(线性分子)：500～10000
详细介绍

葡聚糖凝胶G-50Sephadex G-50

CAS号：9048-71-9

 

英文名称：Sephadex G-50
其他名称：交联葡聚糖凝胶G-50
CAS号：9048-71-9
干颗粒直径：40～120um
分子量分级范围：A.肽及球形蛋白质：1500～30000；B. 葡聚糖（线性分子）：500～10000
床体积毫升/克干分子筛：9～11ml/g
得水值：5.0±0.3
溶胀zui少平衡时间（h）：室温6h（20℃）；沸水浴2h（90℃）
PH：2～13
性状：白色珠粒，属亲水性凝胶。性稳定，不溶于水、弱酸和碱溶液，遇强酸能使糖苷键分解

葡聚糖凝胶G-50Sephadex G-50

Sephadex G-50 Medium, 100 g

Sephadex G-50 Medium is well established gel filtration medium for desalting and buffer exchange of biomolecules >30 000 molecular weight. The larger bead size of G-50 Medium is suited for large scale separations.

  • Quickly desalts, removes contaminants and transfers to a new buffer in a single step.
  • Suitable for purification of DNA from small molecules by gel filtration
  • Larger bead size for large scale group separations where high flow rates and low operating pressures are required.
  • Classic gel filtration medium.

 

Sephadex is a gel filtration medium prepared by crosslinking dextran with epichlorohydrin. Different types of Sephadex differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex G-50 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex G-50 is available in 4 different particle sizes (Coarse, Medium, Fine & Superfine) and Superfine has the smallest bead size for higher efficiency with shorter diffusion distances. Coarse and Medium are are preferred for large scale group separations where high flow rates and low operating pressures are required.

 

Pharmcia（GE）葡聚糖凝胶G-75Pharmcia17-0050-01

发表于由

Pharmcia（GE）葡聚糖凝胶G-75

  • 产品型号：  Pharmcia17-0050-01
  • 简单描述
  • Pharmcia（GE)葡聚糖凝胶G-75葡聚糖凝胶G-75，交联葡聚糖凝胶G-75，交联右旋糖酐凝胶G-75，Sephadex G-75 分离:肽及球形蛋白质：3000～80000；葡聚糖(线性分子)：1000～50000
详细介绍

Pharmcia（GE)葡聚糖凝胶G-75

Sephadex G-75, 100 g

Sephadex G-75 is well established gel filtration medium for desalting and buffer exchange of large biomolecules >80 000 molecular weight.

  • Quickly desalts, removes contaminants and transfers to a new buffer in a single step.
  • Suitable for buffer exchange and cleanup of large biomolecules, >80 000 molecular weight
  • Highest exclusion limit, 80 000, in the Sephadex G-range
  • Classic gel filtration medium.

 

Sephadex is a gel filtration medium prepared by crosslinking dextran with epichlorohydrin. Different types of Sephadex differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex G-10 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex G-50 is available in 4 different particle sizes (Coarse, Medium, Fine & Superfine) and Superfine has the smallest bead size for higher efficiency with shorter diffusion distances. Coarse and Medium are are preferred for large scale group separations where high flow rates and low operating pressures are required.

Pharmcia（GE)葡聚糖凝胶G-75

Sephadex G-75, 100 g

Matrix Cross-linked dextran
Particle Size Wet 90 µm-280 µm
Partical Size Dry 40 µm-120 µm
pH Stability Working Range 2-10
Flow Rate < Darcy´s Law1)
Pressure/Flow Specification min 90 cm/h, bed height 10 cm, column 5 cm i.d.
Fractionation [Mr] Globular Proteins 3 x 103 – 8 x 104
Fractionation [Mr] Dextrans 1 x 103-5 x 104
Exclusion Limit > 7 x 104
Storage Conditions 4 to 30°C
Autoclavable In wet form (pH 7.0) at 120°C for 30 min
1)The beads behave as rigid spheres obeying Darcy's Law.

Pharmcia原装葡聚糖凝胶G-100Sephadex G-100Pharmcia17-0060-01

发表于由

Pharmcia原装葡聚糖凝胶G-100Sephadex G-100

  • 产品型号：  Pharmcia17-0060-01
  • 简单描述
  • Pharmcia原装葡聚糖凝胶G-100Sephadex G-100别名：葡聚糖凝胶G-100，规格：分离：肽及球形蛋白质：4000～150000；葡聚糖(线性分子)：1000～100000包装：10克100克CAS号：9050-94-6保存条件：RT
详细介绍

Pharmcia原装葡聚糖凝胶G-100Sephadex G-100

Matrix Cross-linked dextran
Particle Size Wet 100 µm-310 µm
Partical Size Dry 40 µm-120 µm
Pressure/Flow Specification Care must be taken to avoid compressing the gel. Do not increase the pressure beyond 0.096 bar.
Fractionation [Mr] Globular Proteins 4 x 103-1.5 x 105
Fractionation [Mr] Dextrans 1 x 103-1 x 105
Exclusion Limit > 1.5 x 105
Autoclavable In wet form (pH 7.0) at 120°C for 30 min

Sephadex G-100, 100 g

Application note
Purification and renaturation of recombinant proteins produced in Escherichia coli as inclusion bodies Summary

Expression of eukaryotic genes in the cytoplasm of Escherichia coli often leads to the production and accumulation of the recombinant protein (r-protein) to levels of total cell protein that are higher than when secreted, in some instances by as much as 25%. The r-proteins so produced are frequently in the form of insoluble aggregates, so-called inclusion bodies (IBs). The IBs contain reduced and denatured proteins lacking biological activity. Their sizes and densities are also distinct from most of the bacterial proteins, which facilitates their separation from intracellular E. coli proteins and cell debris by slow speed centrifugation. A general protocol for the extraction and purification of IBs from E. coli cells is shown. The purified IBs are usually dissolved in 6 M guanidine hydrochloride or 8 M urea at near neutral pH prior to re-naturation and purification of the r-protein. The most commonly used procedure for refolding of such de-natured r-proteins is slow dialysis, or dilution into a buffer of near neutral pH. This result in a significant dilution of the r-protein, typically to a few mg of r-protein per mL of solution, with the formation of precipitated proteins being often unavoidable. Alternative purification and/or refolding procedures using gel filtration, ion exchange or hydrophobic interaction chromatography (HIC) have been developed to circumvent such problems. These are outlined briefly in this Application Note. Close
Handbook
Size Exclusion Chromatography: Principles and Methods Summary

This handbook describes the use of SEC for the purification and separation of biomolecules, with a focus on practical information for obtaining the best results. The media available, selection criteria and examples with detailed instructions for the most common applications are included, as well as the theoretical principles behind the technique. The first step towards a successful separation is to select the correct medium and this handbook focuses on the most up-to-date SEC media and prepacked columns. Close
Magazine
Upstream Downstream issue 2

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Material Safety Data Sheet
Sephadex™ G-100, 100 g

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Sephadex™ G-100, 100 g

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Pharmcia原装葡聚糖凝胶G-100Sephadex G-100

17-0060-01 葡聚糖凝胶G-100 Sephadex G-100
17-0050-01 葡聚糖凝胶G-75 Sephadex G-75
17-0043-01 葡聚糖凝胶G-50 Sephadex G-50
17-0033-01 葡聚糖凝胶G-25[中] Sephadex G-25
17-0020-01 葡聚糖凝胶G-15 Sephadex G-15
17-0010-01 葡聚糖凝胶G-10 Sephadex G-10
B-2261 荧光染糖[NO.33342] bisBenzimide H 33342 trihydrochloride
17-0170-01 DEAE葡聚糖凝胶A-25 DEAE Sephadex A-25
17-0170-02 DEAE葡聚糖凝胶A-25 DEAE Sephadex A-25
17-0180-01 DEAE葡聚糖凝胶A-50 DEAE Sephadex A-50
17-0180-02 DEAE葡聚糖凝胶A-50 DEAE Sephadex A-50
G-9382 明胶 Gelatin
L-5125 N-十二烷基肌氨酸钠 N-Lauroylsarcosine sodium salt
L-5125 N-十二烷基肌氨酸钠 N-Lauroylsarcosine sodium salt
0719 N-十二烷基肌氨酸钠 N-Lauroylsarcosine sodium salt
0719 N-十二烷基肌氨酸钠 N-Lauroylsarcosine sodium salt
T-8768 3,3'5,5'-四甲基联苯胺二盐酸 TMB 2Hcl
T-8768 3,3'5,5'-四甲基联苯胺二盐酸 TMB 2Hcl
0759 3,3'5,5'-四甲基联苯胺二盐酸 TMB 2Hcl
0759 3,3'5,5'-四甲基联苯胺二盐酸 TMB 2Hcl
E-2129 埃文斯蓝[伊文斯蓝] Evans Blue
E-2129 埃文斯蓝[伊文斯蓝] Evans Blue
19555 埃文斯蓝[伊文斯蓝] Evans Blue
19555 埃文斯蓝[伊文斯蓝] Evans Blue
0335 蔗糖 Sucrose
21938 蔗糖 Sucrose
F-0127 D-果糖 D-(-)Fructose
0226 D-果糖 D-(-)Fructose
0528 结晶紫 crystal violet
0671 D-核糖 D-Ribose
J392 棉子糖 D-Raffinose
P3504 丽春红S Ponceau S
0860 丽春红S Ponceau S
C-2037 细胞色素C[牛心] Cytochromes C(Bovine Heart)
C-7752 细胞色素C[马心] Cytochromes C(Horse Heart)
0322   EDTA
0322   EDTA
D-4551 N,N-二甲基甲酰胺 N,N-Dimethylformamide
0464 N,N-二甲基甲酰胺 N,N-Dimethylformamide
T-9250 胸腺嘧啶核苷 Thymidine
T-9250 胸腺嘧啶核苷 Thymidine
22305 胸腺嘧啶核苷 Thymidine
22305 胸腺嘧啶核苷 Thymidine
P-3250 木瓜蛋白酶 Papain from papaya latex
P-3250 木瓜蛋白酶 Papain from papaya latex
G-3632 硫酸庆大霉素 Gentamicin sulfate salt
G-3632 硫酸庆大霉素 Gentamicin sulfate salt
0304 硫酸庆大霉素 Gentamicin sulfate salt
0304 硫酸庆大霉素 Gentamicin sulfate salt
C-7698 [环已亚胺] Cycloheximide
C-7698 [环已亚胺] Cycloheximide
D-1756 地塞米松（氢化可的松） Dexamethasone
D-1756 地塞米松（氢化可的松） Dexamethasone
A-7506 维生素C[抗球血酸钠] L-Ascorbic acid[Vitamin C]
A-7506 维生素C[抗球血酸钠] L-Ascorbic acid[Vitamin C]
0764 维生素C[抗球血酸钠] L-Ascorbic acid[Vitamin C]
0764 维生素C[抗球血酸钠] L-Ascorbic acid[Vitamin C]
 ******* 维生素C[抗球血酸钠] L-Ascorbic acid[Vitamin C]
 ******* 氯化锂 Lithium chloride
0416 氯化锂 Lithium chloride
22178 纤维素酶 Cellulase from Aspergillus niger
**** 纤维素酶 Cellulase from Aspergillus niger
M-8410 矿物油[石腊油] Mineral oil
J217 矿物油[石腊油] Mineral oil
C-7880 L-半胱氨酸盐酸盐 L-Cysteine hydrochloride monohydrate
C-7880 L-半胱氨酸盐酸盐 L-Cysteine hydrochloride monohydrate
0206 L-半胱氨酸盐酸盐 L-Cysteine hydrochloride monohydrate
0206 L-半胱氨酸盐酸盐 L-Cysteine hydrochloride monohydrate
E-4378 乙二醇-双-(2-氨基乙基)四乙酸 Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid[EGTA]
0732 乙二醇-双-(2-氨基乙基)四乙酸 Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid[EGTA]
T-8877 2,3,5-氯化三苯基四氮唑 2,3,5-Triphenyltetrazolium chloride[TTC]
0765 2,3,5-氯化三苯基四氮唑 2,3,5-Triphenyltetrazolium chloride[TTC]
B-0252 硼酸 Boric acid
0588 硼酸 Boric acid
P-3640 聚乙二醇 3350 PEG 3350
P-3640 聚乙二醇 3350 PEG 3350
817006 聚乙二醇 4000 PEG 4000
817006 聚乙二醇 4000 PEG 4000
807491 聚乙二醇 6000 PEG 6000
807491 聚乙二醇 6000 PEG 6000
0159 聚乙二醇 8000 PEG 8000
0159 聚乙二醇 8000 PEG 8000
81285 聚乙二醇 12000 PEG 12000
81285 聚乙二醇 12000 PEG 12000
818897 聚乙二醇 20000 PEG 20000
818897 聚乙二醇 20000 PEG 20000
K-3378 激动素[6-呋喃氨基嘌呤] 6-Furfuryl Aminopurine(Kinetin)[KT]
K-3378 激动素[6-呋喃氨基嘌呤] 6-Furfuryl Aminopurine(Kinetin)[KT]
17980 激动素[6-呋喃氨基嘌呤] 6-Furfuryl Aminopurine(Kinetin)[KT]
17980 激动素[6-呋喃氨基嘌呤] 6-Furfuryl Aminopurine(Kinetin)[KT]
N-3503 制霉菌素 Nystatin
N-3503 制霉菌素 Nystatin
19770 制霉菌素 Nystatin
19770 制霉菌素 Nystatin
D-5637 3,3'-二氨基联苯胺 3,3′-Diaminobenzidine tetrahydrochloride hydrate[DAB]
D-5637 3,3'-二氨基联苯胺 3,3′-Diaminobenzidine tetrahydrochloride hydrate[DAB]
0430 3,3'-二氨基联苯胺 3,3′-Diaminobenzidine tetrahydrochloride hydrate[DAB]
0430 3,3'-二氨基联苯胺 3,3′-Diaminobenzidine tetrahydrochloride hydrate[DAB]
I-2886 吲哚-3-乙酸 3-Indoleacetic acid[IAA]
I-2886 吲哚-3-乙酸 3-Indoleacetic acid[IAA]
17550 吲哚-3-乙酸 3-Indoleacetic acid[IAA]
17550 吲哚-3-乙酸 3-Indoleacetic acid[IAA]
A-5006 L-精氨酸 L-Arginine
A-5006 L-精氨酸 L-Arginine
11490 L-精氨酸 L-Arginine
11490 L-精氨酸 L-Arginine
M-9625 L-甲硫氨酸[L-蛋氨酸] L-Methionine
M-9625 L-甲硫氨酸[L-蛋氨酸] L-Methionine
18915 L-甲硫氨酸[L-蛋氨酸] L-Methionine
18915 L-甲硫氨酸[L-蛋氨酸] L-Methionine
G-8270 D-葡萄糖[无水] D-(+)-Glucose
0188 D-葡萄糖[无水] D-(+)-Glucose
F-9037 去离子甲酰胺 Formamide,Deionized
0606 去离子甲酰胺 Formamide,Deionized

葡聚糖凝胶G-200Sephadex G-200Pharmacia 17-0081-01

发表于由

葡聚糖凝胶G-200Sephadex G-200

  • 产品型号：  Pharmacia 17-0081-01
  • 简单描述
  • 葡聚糖凝胶G-200Sephadex G-200英文名称：Sephadex G-200；Cross-inked dextran gel G-200其他名称：交联葡聚糖凝胶G-200咨询
详细介绍

葡聚糖凝胶G-200Sephadex G-200

英文名称：Sephadex G-200；Cross-inked dextran gel G-200
其他名称：交联葡聚糖凝胶G-200
干颗粒直径：40～120um（细级）
分子量分级范围：A.肽及球形蛋白质：5000～800000；B. 葡聚糖（线性分子）：1000～200000
床体积毫升/g干分子筛：30～40ml/g
得水值：20.0±2.0
溶胀zui少平衡时间（h）：室温72h（20℃）；沸水浴5h（90℃）
柱头压力（kpa）（2.5cm直径柱）：0.39～1.57
PH：2～13
性状：白色珠粒，属亲水性凝胶。性稳定，不溶于水、弱酸和碱溶液，遇强酸能使糖苷键分解
用途：生化研究。用于凝胶过滤，肽类分离、脱盐，分子量测定
保存：RT

葡聚糖凝胶G-200Sephadex G-200

GE Pharmacia蓝色葡聚糖2000 17-0360-0117-0360-01

发表于由

GE Pharmacia蓝色葡聚糖2000 17-0360-01

  • 产品型号：  17-0360-01
  • 简单描述
  • GE Pharmacia蓝色葡聚糖2000产品名称： 蓝色葡聚糖 2000英文名称： Dextran Blue 2000产品货号： 17-0360-01产品规格： 10g产品商标： Pharmacia
详细介绍
GE Pharmacia蓝色葡聚糖2000 17-0360-0117-0360-01
通用电气生命科学部

GE Pharmacia蓝色葡聚糖2000
产品名称： 蓝色葡聚糖 2000
英文名称： Dextran Blue 2000
产品货号： 17-0360-01
产品规格： 10g
产品商标： Pharmacia
 

GE Pharmacia葡聚糖凝胶G-10 17-0010-0117-0010-01

发表于由

GE Pharmacia葡聚糖凝胶G-10 17-0010-01

  • 产品型号：  17-0010-01
  • 简单描述
  • GE Pharmacia葡聚糖凝胶G-10 17-0010-01 100g Pharmacia葡聚糖凝胶G-10 17-0010-02 500g Pharmacia葡聚糖凝胶G-15 17-0020-01 100g Pharmacia葡聚糖凝胶G-15 17-0020-02 500g
详细介绍

SEPHADEX系列：
GE Pharmacia葡聚糖凝胶SEPHADEX G-10 17-0010-01 100g
   Pharmacia葡聚糖凝胶SEPHADEX G-10 17-0010-02 500g
   Pharmacia葡聚糖凝胶SEPHADEX G-15 17-0020-01 100g
   Pharmacia葡聚糖凝胶SEPHADEX G-15 17-0020-02 500g

   Pharmacia葡聚糖凝胶SEPHADEX G-25 17-0033-01 100g（中等颗粒）
   Pharmacia葡聚糖凝胶SEPHADEX G-25 17-0033-02 500g（中等颗粒）
   Pharmacia葡聚糖凝胶SEPHADEX G-25 17-0034-01 100g（粗颗粒）
   Pharmacia葡聚糖凝胶SEPHADEX G-25 17-0034-02 500g（粗颗粒）

   Pharmacia葡聚糖凝胶SEPHADEX G-50 17-0043-01 100g（中等颗粒）
   Pharmacia葡聚糖凝胶SEPHADEX G-50 17-0043-02 500g（中等颗粒）
   Pharmacia葡聚糖凝胶SEPHADEX G-50 17-0044-01 100g（粗颗粒）
   Pharmacia葡聚糖凝胶SEPHADEX G-50 17-0044-02 500g（粗颗粒）

   Pharmacia葡聚糖凝胶SEPHADEX G-75 17-0050-01 100g
   Pharmacia葡聚糖凝胶SEPHADEX G-75 17-0050-02 100g
   
   Pharmacia葡聚糖凝胶SEPHADEX G-100 17-0061-01 100g
   Pharmacia葡聚糖凝胶SEPHADEX G-100 17-0061-02 500g

   Pharmacia葡聚糖凝胶SEPHADEX LH-20 17-0090-10 25g
   Pharmacia葡聚糖凝胶SEPHADEX LH-20 17-0090-01 100g
   Pharmacia葡聚糖凝胶SEPHADEX LH-20 17-0090-02 500g
   
   Pharmacia DEAE葡聚糖凝胶SEPHADEX A-25   17-0170-01 100g
   Pharmacia DEAE葡聚糖凝胶SEPHADEX A-25   17-0170-02 500g
 
   Pharmacia DEAE葡聚糖凝胶SEPHADEX A-50   17-0180-01 100g
   Pharmacia DEAE葡聚糖凝胶SEPHADEX A-50   17-0180-02 500g

 

GE 葡聚糖凝胶SEPHADEX LH-2017-0090-01

发表于由

GE 葡聚糖凝胶SEPHADEX LH-20

  • 产品型号：  17-0090-01
  • 简单描述
  • GE 葡聚糖凝胶SEPHADEX LH-2025G、100G、500G装*Sephadex™ LH-20优秀的脂类，激素，维生素，和其他生物小分子分馏。专为在极性有机溶剂和水溶剂混合物中使用。
详细介绍

GE 葡聚糖凝胶SEPHADEX LH-20
 

Sephadex™ LH-20

  • Excellent for fractionation of lipids, hormones, vitamins, and other small biomolecules. Designed for use in polar organic solvents and aqueous solvent mixtures.
  • Since Sephadex™ LH-20 has exceptional properties, we recommend that you consult the publications listed under Related Products.

葡聚糖™LH – 20技术资料：
 

Sephadex™ LH-20

Technical Information

TECHNICAL SPECIFICATIONS
矩阵 羟丙基，交联葡聚糖
珠形式 球形，多孔
粒度范围（直径）  
dry  18–111 μm 
在甲醇中 27–163 μm 
pH稳定性 2–13 （working and cleaning） 
化学稳定性 在水相和有机洗脱液系统的稳定。
高压灭菌  在121 ° C 温度下灭菌20分钟

 

TECHNICAL SPECIFICATIONS
Maximum linear flow rate  ~12 cm/min 
Recommended linear flow rate  ~1 cm/min but depends on resolution required 
Operating temperature  4°C to 40°C 
Sample loading volumes   
adsorption mode  determined by desired degree of resolution 
Molecular sizing  ~2% total bed volume 
Partition mode  ~1% total bed volume 
Exclution limit*  peptides ~4 kDa, small organics ~5 kDa 
Shelf-life  5 years 
Recommended storage  Maintain dry at 4°C to 25°C, wet 4°C to +8°C, pH 6–8, with bacteriostat 
* Depends on solvent in which the medium has been equilibrated.