Efficient: no background effects like cell activation or apoptosis
Flexible: tested for variety of tissue types
The MACS® Tissue Storage Solution has been developed to allow an optimized storage of fresh organ and tissue samples without showing background effects like cell activation or apoptosis induction. It has been tested and validated on a variety of human and murine tissues including tumors, skin, heart, spleen, brain, and skeletal muscle.
IL-2 IS stands for interleukin 2 “Improved Sequence”, also termed aldesleukin, and is a variant of IL-2 with a serine to cysteine substitution. Human IL-2 IS is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays, and possesses the same properties as naturally occurring IL-2.
Background information
IL-2, a potent lymphoid cell growth factor, is a typical four α-helix bundle cytokine. It is produced by activated T cells, especially the CD4+ T helper cell population. It plays an important role in both the activation and maintenance of immune responses and in lymphocyte development. IL-2 promotes proliferation and differentiation of T cells, NK cells and B cells and is involved in the elimination of self-reactive T cells. IL-2 signals through a receptor complex consisting of IL-2 receptor α-chain (CD25), β- chain, and common γ-chain. The latter two are also used for IL-15 signaling.
Biological activity
Proliferation of CTLL-2 cells (NIBSC 86/504)
research grade: ≥ 3×106 IU/mg
premium grade: ≥ 5×106 IU/mg
Quality description
Research-grade cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).
miltenyi 美天旎磁珠CD25 MicroBeads II, human – lyophilized
CD25 MicroBeads II were developed for positive selection or depletion of CD25+ cells.
miltenyi 美天旎磁珠CD25 MicroBeads II, human – lyophilized
Background information
CD25, the low-affinity interleukin-2 receptor alpha chain (IL-2Rα), is expressed on activated T and B cells and monocytes. CD25 is also present on a subset of non-activated CD4+ T cells, which has regulatory function.
The CD25 MicroBeads II recognize epitope A of the CD25 molecule.
For control staining of cells separated with CD25 MicroBeads II, it is recommended to use MACS® CD25 Antibodies, which bind to epitope B of the CD25 antigen.
For isolation of CD25+ cell subsets, the CD25 MicroBeads II can be combined with MACS® Cell Isolation Kits or MultiSort Kits. The CD4+CD25+ Regulatory T Cell Isolation Kit is recommended for most efficient separation of CD4+CD25+ regulatory T cells.
Columns
For positive selection: MS, LS, XS, or autoMACS® Columns. For depletion: LD, CS, D, or autoMACS Columns.
miltenyi美天旎human IL-15蛋白,人白介15细胞生长因子IL-15 stands for interleukin 15. Human IL-15 is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.
miltenyi 美天旎 磁珠及其他分选产品 130-094-531 Diamond CD34 Isolation Kit, human
The Diamond CD34 Isolation Kit has been specifically developed for the isolation of highly pure Lin–CD34+ hematopoietic stem cells from peripheral blood1, bone marrow2,3 or cord blood.
MACS® NeuroBrew®-21 (formerly MACS Supplement B27 PLUS) are serum-free neural supplements designed for use in combination with MACS Neuro Medium for the in vitropropagation of mature neural cells of the central and peripheral nervous system, or neural stem cells of primary or ES/iPS cell origin.
MACS® NeuroBrew-21 is a re-optimized formulation of the B27 components designed to improve performance.1 It has been developed for the in vitro propagation of neural cells of the central and peripheral nervous system.
MACS NeuroBrew-21 has been designed for
optimal growth and long term viability of neural cells of the central and peripheral nervous system
the growth of nearly pure populations of neural cells without the need of an astrocyte feeder layer
MACS NeuroBrew-21 w/o Vitamin A is identical to MACS NeuroBrew-21, but without the addition of Vitamin A or other retinoid related components. Vitamin A induces the differentiation of neural stem cells into mature neural cells. MACS NeuroBrew-21 w/o Vitamin A is suitable for use with studies of neural stem cells, neural development and neural cell differentiation.
MACS NeuroBrew-21 w/o Vitamin A has been designed for use with
primary neural stem or progenitor cells
primary neurospheres
ES/iPS cell derived neural stem cells and neurosphere
The Whole Blood Column Kit is suited for the separation of leukocyte subsets directly from anticoagulated human whole blood or bone marrow. The kit contains Whole Blood Columns and Whole Blood Column Elution Buffer for convenient positive selection of cells labeled with Whole Blood MicroBeads.
Whole Blood Columns are designed for use with:
MidiMACS™ Separator
QuadroMACS™ Separator
VarioMACS™ Separator, in combination with an LS Column Adapter
SuperMACS™ II Separator
MultiMACS Cell24 Separator Plus, in combination with the Single Column Adapter
Saves time, as cells are immediay ready for any downstream application
The NK Cell Isolation Kit was developed for the untouched isolation of NK cells from human PBMCs. Non-NK cells, i.e., T cells, B cells, stem cells, dendritic cells, monocytes, granulocytes, and erythroid cells, are magnetically labeled by using a cocktail of biotin-conjugated antibodies and the NK Cell MicroBead Cocktail. Isolation of highly pure NK cells is achieved by depletion of magnetically labeled cells.
130-091-376 MACS BSA Stock Solution 6 x 75 ml MACS BSA原液6×75毫升
MACS BSA Stock Solution is used for the fast and easy preparation of a preservative-free standard MACS Separation Buffer that is suitable for any MACS Cell Separation System.
Background information
The MACS® BSA Stock Solution consists of phosphate-buffered saline supplemented with 10% bovine serum albumin. By simply diluting it 1:20 with the autoMACS® Rinsing Solution an optimal buffer for cell preparation and separation of magnetically labeled cells is obtained.
The MACS BSA Stock Solution is supplied in a 75 mL bottle format. One bottle is sufficient for the preparation of 1.5 L MACS Separation Buffer. This solution is sterile-filtered and preservative-free. It is quality-controlled for cell separation requirements.
CD138 MicroBeads were developed for the positive selection or depletion of plasma cells from PBMCs, bone marrow, leukapheresis harvests, and single-cell suspensions from lymphoid tissues.
A Mini & MidiMACS™ Starting Kit provides indispensable equipment for MACS® Cell Separations. The Mini & MidiMACS Starting Kit includes a MiniMACS Separator, a MidiMACS Separator, a MultiStand to attach the separators, MACS Columns, and a cell separation reagent of choice.
The decision for one or the other Mini & MidiMACS Starting Kit depends on the preference for LS Columns or LD Columns.
The MiniMACS Separator is used for smaller cell samples, which are separated over MS Columns, whereas larger samples are processed using the MidiMACS Separator in combination with LS or LD Columns.
Product
Order no.
Price
Mini & MidiMACS Starting Kit (MS, LS)
Components:
MiniMACS Separator (130-042-102)
MidiMACS Separator (130-042-302)
MACS MultiStand (130-042-303)
MS Columns (130-042-201)
LS Columns (130-042-401)
One MACS Cell Separation Reagent of choice (includes only reagents with a capacity of up to 2×109 total cells)
he autoMACS® Running Buffer is an optimized MACS® Separation Buffer suitable for MACS Cell Separation on the autoMACS Pro Separator and the autoMACS Separator and for manual MACS Cell Separation Systems which are not sensitive to azide.
Background information
The autoMACS Running Buffer is sterile-filtered and ready-to-use. It contains bovine serum albumin (BSA), EDTA, and 0.09% azide. It is quality-controlled for cell separation requirements. One bottle (1.5 L) is sufficient for up to 15 cycles consisting of cell separation and system rinsing using the autoMACS Instruments.
LS Columns are designed for positive selection of cells. They are also suitable for the depletion of strongly magnetically labeled cells.
LS Columns can be used with the following separators:
MidiMACS™ Separator
QuadroMACS™ Separator
VarioMACS™ Separator, in combination with an LS Column Adapter
SuperMACS™ II Separator
MultiMACS Cell24 Separator Plus, in combination with the Single Column Adapter
miltenyi美天旎磁珠分选柱子LS Columns
Column capacity
For lymphoid cells from blood, bone marrow or tissue:
Up to 108 magnetically labeled cells from up to 2×109 total cells.
For the capacity of cells from other tissues, e.g., neural tissues, please refer to the data sheet provided with the particular MACS Cell Separation Reagent.
For use with the MultiMACS Cell24 Separator Plus:
Up to 108 magnetically labeled cells from up to 1×109 total cells.
Miltenyi美天旎 MS Columns柱子MS Separation columns品牌:Miltenyi(美天旎)货号:130-042-201CAS号:详见说明书供应商:Miltenyi(美天旎)保质期:详见说明书
详细介绍
Miltenyi美天旎 MS Columns柱子
详细说明 样品来源: 样品处理方式: 处理量/容量: 25 columns 产物产量: 总用时: 应用: MS Columns have been developed for positive selection of human and animal cells, especially rare cells, out of a heterogeneous cell suspension in combination with a MACS Separator. 客户评价: 商业评价: