miltenyi 美天旎磁珠CD25 MicroBeads II, human – lyophilized
CD25 MicroBeads II were developed for positive selection or depletion of CD25+ cells.
miltenyi 美天旎磁珠CD25 MicroBeads II, human – lyophilized
Background information
CD25, the low-affinity interleukin-2 receptor alpha chain (IL-2Rα), is expressed on activated T and B cells and monocytes. CD25 is also present on a subset of non-activated CD4+ T cells, which has regulatory function.
The CD25 MicroBeads II recognize epitope A of the CD25 molecule.
For control staining of cells separated with CD25 MicroBeads II, it is recommended to use MACS® CD25 Antibodies, which bind to epitope B of the CD25 antigen.
For isolation of CD25+ cell subsets, the CD25 MicroBeads II can be combined with MACS® Cell Isolation Kits or MultiSort Kits. The CD4+CD25+ Regulatory T Cell Isolation Kit is recommended for most efficient separation of CD4+CD25+ regulatory T cells.
Columns
For positive selection: MS, LS, XS, or autoMACS® Columns. For depletion: LD, CS, D, or autoMACS Columns.
miltenyi 美天旎 磁珠及其他分选产品 130-094-531 Diamond CD34 Isolation Kit, human
The Diamond CD34 Isolation Kit has been specifically developed for the isolation of highly pure Lin–CD34+ hematopoietic stem cells from peripheral blood1, bone marrow2,3 or cord blood.
CD138 MicroBeads were developed for the positive selection or depletion of plasma cells from PBMCs, bone marrow, leukapheresis harvests, and single-cell suspensions from lymphoid tissues.
LS Columns are designed for positive selection of cells. They are also suitable for the depletion of strongly magnetically labeled cells.
LS Columns can be used with the following separators:
MidiMACS™ Separator
QuadroMACS™ Separator
VarioMACS™ Separator, in combination with an LS Column Adapter
SuperMACS™ II Separator
MultiMACS Cell24 Separator Plus, in combination with the Single Column Adapter
miltenyi美天旎磁珠分选柱子LS Columns
Column capacity
For lymphoid cells from blood, bone marrow or tissue:
Up to 108 magnetically labeled cells from up to 2×109 total cells.
For the capacity of cells from other tissues, e.g., neural tissues, please refer to the data sheet provided with the particular MACS Cell Separation Reagent.
For use with the MultiMACS Cell24 Separator Plus:
Up to 108 magnetically labeled cells from up to 1×109 total cells.
•高容量— 结合容量几乎是其他供应商的典型磁珠的 4 倍,每次实验的样品用量更少 •低非特异性结合— 稳定的预封闭磁珠可提供洁净的纯化产物(例如,抗体 IP 中洗脱的抗原中不含来自复合物 IP 基质的污染性蛋白) •灵活性— 一个磁珠上同时含有蛋白 A 和蛋白 G 的 IgG 结合结构域,非常便利 •兼容性— 磁珠兼容手动和自动化应用(例如,Thermo Scientific KingFisher 仪器) •检测一致性— 磁珠可消除树脂损耗,与仅使用微量离心管的传统 IP 方法相比,分离效率更高
这些磁珠包被经基因工程改造的 Pierce 蛋白 A/G,这是一种同时含有蛋白 A 及蛋白 G 的 IgG 结合结构域的重组融合蛋白,与单独使用蛋白 A 或者蛋白 G 相比,可从更广泛的物种和同种型范围中捕获抗体。使用我们的化学交联剂,您可以将抗体固定到磁粒子上,避免免疫沉淀样品受到 IgG 污染。这些微珠可与磁力架配合用于手工操作,亦可用于自动化平台(如 Thermo Scientific KingFisher 仪器)。
应用: • IP 和 Co-IP 实验(参见全套试剂盒) •用于非还原条件下分析的免疫沉淀 • 抗体纯化
Pierce 磁珠上固定的重组蛋白 A/G 由蛋白 A 和蛋白 G 的 IgG 结合结构域相融合而成。蛋白 A/G 包含蛋白 A 的四个 Fc 结合结构域及蛋白 G 的两个 Fc 结合结构域,使其成为研究及纯化免疫球蛋白的便利工具。因此,Pierce 磁粒子并不是简单地将蛋白 A 及蛋白 G 多肽进行混合后固定,更不是蛋白 A 磁珠与蛋白 G 磁珠的混合物。
For Research Use Only. Not for use in diagnostic procedures.
LIFE公司(代理)磁珠Dynabeads® His-Tag Isolation and Pulldown货号: 10103D规格:2ml
详细介绍
LIFE公司(代理)磁珠
描述
This product is designed for his-tagged protein isolation. High selectivity for poly-histidine tags results in extremely low background levels.
The new bead surface chemistry is Cobalt-based and offers exceptionally fast binding kinetics and a high yield per microliter of beads. The entire protocol (from cleared lysate to purified protein) takes only 20 minutes! The protocol includes instructions for making the required buffers as well as methods for optimizing buffer composition.
Dynabeads® His-Tag Isolation & Pulldown replaces Dynabeads® TALON™. Dynabeads® His-Tag Isolation & Pulldown has a new optimized binding chemistry that offers significant advantages over Dynabeads® TALON™ which were coated in the TALON™ chemistry. This optimization results in a cleaner prep with higher yield of his-tag protein and extremely low background levels.
Dynabeads His-Tag Isolation & Pulldown is designed for isolating His-tagged proteins from cleared lysates. Isolated his-tagged proteins can either be eluted from the beads or kept on the beads for use in bead-based downstream assays. Bead bound his-tagged proteins can be used in various downstream assays such as bait-prey pulldowns, etc.
Note: This product contains Dynabeads and a protocol only, and does not include buffers.
The technology used for the discontinued Dynabeads® TALON™ was licensed from Clontech. TALON™ is a registered trademark of Clontech Laboratories Inc., USA.