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Biolegend Human CD28单抗（细胞治疗级） 货号302914302913

发表于由

Biolegend Human CD28单抗（细胞治疗级） 货号302914

  • 产品型号：  302913
  • 简单描述
  • Biolegend Human CD28单抗（细胞治疗级） 货号302914规格：302913 规格：50ug302914 规格：500ug302923 规格：1mg现货供应
详细介绍

Biolegend Human CD28单抗（细胞治疗级） 货号302914

通用名称：T淋巴细胞活化用抗人CD28单抗

Biolegend Human CD28单抗（细胞治疗级） 货号302914

CD28分子存在于大多数T细胞表面，被认为是一种T细胞*的表面分子。T细胞对抗原的反应性主要通过CD3/TCR复合物介导，但也有赖于T细胞表面其他分子的协同。ＣＤ２８与其天然配基Ｂ７结合可发挥共刺激作用，作为第二信号介绍Ｔ细胞激活，促进Ｔ细胞增殖及分泌多种细胞因子并介绍导Ｔ，Ｂ细胞粘附。在体外T淋巴细胞激活扩增中抗人CD28单克隆抗体通过第二信号通路发挥共刺激作用。

简单信息：

Clone: CD28.2 (See other available formats)
Isotype: Mouse IgG1, κ
Isotype Control: LEAF™ Purified Mouse IgG1, κ Isotype Ctrl
Workshop
Number:		 V-CD28.05
Reactivity: Human, Cross-Reactivity: Chimpanzee, Baboon, Cynomolgus, Rhesus, Pigtailed Macaque, Squirrel Monkey, Capuchin Monkey, Sooty Mangabey
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.1 EU/µg of the protein (<0.01 ng/µg of the protein) as determined by the LAL test.
Preparation: The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: 1.0 mg/ml

订购信息：

302913 规格：50ug
302914 规格：500ug
302923 规格：1mg

Biolegend LEAF Purified Human CD28单抗302913302914

发表于由

Biolegend LEAF Purified Human CD28单抗302913

  • 产品型号：  302914
  • 简单描述
  • Biolegend LEAF Purified Human CD28单抗302913302913 规格：50ug302914 规格：500ug302923 规格：1mg现货！
详细介绍

Biolegend LEAF Purified Human CD28单抗302913

Clone: CD28.2 (See other available formats)
Isotype: Mouse IgG1, κ
Isotype Control: LEAF™ Purified Mouse IgG1, κ Isotype Ctrl
Workshop
Number:		 V-CD28.05
Reactivity: Human, Cross-Reactivity: Chimpanzee, Baboon, Cynomolgus, Rhesus, Pigtailed Macaque, Squirrel Monkey, Capuchin Monkey, Sooty Mangabey
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.1 EU/µg of the protein (<0.01 ng/µg of the protein) as determined by the LAL test.
Preparation: The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: 1.0 mg/ml

Biolegend LEAF Purified Human CD28单抗302913

Storage & Handling: The CD28 antibody solution should be stored undiluted between 2°C and 8°C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: FC – Quality tested
Costim, IP, IHC – Reported in the literature
Recommended Usage: Each lot of this antibody is quality control tested byimmunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Application
Notes:		 Additional reported applications (for the relevant formats) include: immunoprecipitation, immunohistochemical staining of acetone-fixed frozen tissue sections4, and in vitro T cell costimulation5-8. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The CD28.2 antibody co-stimulates T cell proliferation and cytokine production in the presence of suboptimal amounts of anti-CD3 antibody. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 302914). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 302934) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Application
References:		 1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. 
2. Nunes J, et al. 1993. Biochem. J. 293:835. 
3. Calea-Lauri J, et al. 1999. J. Immunol. 163:62.
4. Tazi A, et al. 1999. J. Immunol. 163:3511. (IHC)
5. Marti F, et al. 2001. J. Immunol. 166:197. (Costim)
6. Jeong SH, et al. 2004. J. Virol. 78:6995. (Costim)
7. Rivollier A, et al. 2004. Blood 104:4029. (Costim)
8. Scharschmidt E, et al. 2004. Mol. Cell Biol. 24:3860. (Costim)
9. Sheng W, et al. 2007.Elsevier 580:6819. PubMed
10. Mitsuhashi M. 2007. Clin Chem.53:148. PubMed
11. Ye Z, et al. 2008. Infect. Immun. 76:2541. PubMed
12. Magatti M, et al. 2008. Stem Cells 26:182. (FA) PubMed
  Publication Library
Biolegend LEAF Purified Human CD28单抗302913302914

Human peripheral blood lymphocytes stained with LEAF™ purified CD28.2, followed by anti-mouse IgGs FITC

Biolegend LEAF Purified Human CD28单抗302913302914

Human peripheral blood mononuclear cells were stained with CFSE on day 0, and then stimulated with (filled histogram) or without (open histogram) immobilized LEAF™ Purified CD3 (clone UCHT1) and LEAF™ purified CD28 (clone CD28.2) for 3 days. On day 4, cells were harvested and stained with CD4 Brilliant Violet 711™. Dot plot (above) was analyzed on live cells. Histogram data (below) was analyzed by gating on CD4 positive cells.

Biolegend LEAF Purified Human CD28单抗302913302914

Compare all formats

 
 
 

Antigen Details
 
Description: CD28 is a 44 kD disulfide-linked homodimeric type I glycoprotein. It is a member of the immunoglobulin superfamily and is also known as T44 or Tp44. CD28 is expressed on most T lineage cells, NK cell subsets, and plasma cells. CD28 binds both CD80 and CD86 using a highly conserved motif MYPPY in the CDR3-like loop. CD28 is considered a major co-stimulatory molecule, inducing T lymphocyte activation and IL-2 synthesis, and preventing cell death. In vitro studies indicate that ligation of CD28 on T cells by CD80 and CD86 on antigen presenting cells provides a costimulatory signal required for T cell activation and proliferation.
Other Names: T44, Tp44
Structure: Ig superfamily, type I transmembrane glycoprotein, homodimer, 44 kD
Distribution: Mature T cells, thymocytes, NK cell subsets, plasma cells, EBV-positive B cells
Function: T cell costimulation
Ligand Receptor: CD80, CD86
Antigen
References:		 1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. 
2. June CH, et al. 1994. Immunol. Today 15:321. 
3. Linskey PS, et al. 1993. Annu. Rev. Immunol. 11:191.
GeneID: 940 Biolegend LEAF Purified Human CD28单抗302913302914
UniProt: View information about CD28 on UniProt.org
Keywords: LEAF™ Purified anti-human CD28, CD28.2, LEAF™, T44, Tp44, Human, Cross-Reactivity: Chimpanzee, Baboon, Cynomolgus, Rhesus, Pigtailed Macaque, Squirrel Monkey, Capuchin Monkey, Sooty Mangabey, Activation, Costimulation, Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Biofunctional Assay, Immunology, Antibodies

订购信息：

302913 规格：50ug
302914 规格：500ug
302923 规格：1mg

现货*！