Invitrogen miRNA 分离试剂盒内容与储存• 30 mL miRNA 洗涤液 I(室温)• 50 mL 洗涤液 2/3(室温)• 80 个收集管(室温)• 40 个滤芯(室温)• 100 mL 裂解/结合缓冲液 (4°C)• 10 mL miRNA 匀浆添加剂 (4°C)• 1.4 mL 凝胶上样缓冲液 II (-20°C)
STEMCELL淋巴细胞分离液LymphoprepLymphoprep分离液Lymphoprep是一种无菌、内毒素含量低的即用型分离液,是目前国内外分离正常人或病人外周血单个核细胞(PBMC:Mononuclear cells of Peripheral blood)(包括单核细胞monocytes和淋巴细胞lymphocytes)的常用方法,具有与昂贵的GE公司的PLUS或PREMIUM分
注意事项: 1、该分离液仅适用于人体的外周血液,不适用于动物。 2、使用该分离液时,需将待分离的血液用生理盐水做等体积的稀释。 3、分离液的保存应置于无菌环境,并避光保存。 4、详细操作步骤见产品说明书。 技术参数 成分:泛影葡胺钠(Sodium diatrizoate):9.1% (w/v);多糖:5.7% (w/v) 密度:1.077±0.001g/ml 渗透性:290±15mOsm 内毒素含量:<1.0EU/ml 分离所得PBMC中红细胞的含量:整个细胞数目的3-10%。 Lymphoprep™ is a density gradient medium recommended for the isolation of mononuclear cells from peripheral blood, cord blood, and bone marrow by exploiting differences in cell density. Granulocytes and erythrocytes have a higher density than mononuclear cells and therefore sediment through the Lymphoprep™ layer during centrifugation. Lymphoprep can be substituted for Ficoll-Paque™ without any need to change your existing protocols and is fully compatible with both Sepmate™ and RosetteSep™.
Product Type:
Cell isolation products
Recommended For:
Isolation of mononuclear cells from peripheral blood, cord blood, and bone marrow
Components:
• Sodium Diatrizoate 9.1% (w/v)
• Polysaccharide 5.7% (w/v)
• Water
• Density of 1.077 g/mL
Accessory Products:
SepMate™-50 (Catalog #15450/15460)
Contains:
• Sodium Diatrizoate (9.1% w/v)
• Polysaccharide (5.7% w/v)
• Density of 1.077 g/mL
Equipment Required:
Centrifuge
Cell Isolation Product Type:
Reagents
Cell Source:
Bone marrow, Cord blood, Whole blood
Legal Statement:
Lymphoprep™ is a trademark of Axis-Shield.
Intended Use Statement:
FOR RESEARCH USE ONLY. NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES.
ISO Statement:
STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485 MEDICAL DEVICE STANDARDS.