激活ASK1检测抗体

产品编号

产品名称【中文名称】

规格

包装

017-22351

Anti Phosphorylated ASK1, Monoclonal   Antibody

【抗磷酸化ASK1，单克隆抗体】

免疫化学用

50μg

抗体信息

抗原名

ASK1　pThr838

适用实验

WB

同种型

IgG

WB图像

（ – ）泳道：对照质粒

TA泳道：过度表达磷酸化抗体识别部位苏氨酸838使Ala丙氨酸变异的人ASK1。

WT泳道：过度表达野生型人ASK1

Stim泳道：过度表达野生型人ASK1，H₂O₂刺激激活。

数据提供：东京大学大学院药学研究科 丸山顺一 野口拓也

抗原信息

第838苏氨酸磷酸化的人ASK 1 835   – 845氨基酸序列肽

物种交叉反应性

人，小鼠

标签

非标签

抗原别名

Apoptosis Signal Regulating Kinase 1
  ※ASK1的别名
  MAP3K5, MEKK5, MAPKKK5

免疫动物

小鼠

克隆号

PA214

详细信息

ASK1是位于MAP激酶通路最上游的细胞内蛋白质磷酸酶。通过应激刺激使活性酶细胞被激活，第838苏氨酸磷酸化而被激活。诱导凋亡和细胞分化。另一方面有报道称，ASK1活化性可诱导阿尔茨海默症和ALS神经细胞凋亡。

本产品是识别第838苏氨酸磷酸化ASK 1的单克隆抗体。

使用文献

1. Ichijo, H., Nishida, E., Irie, K., et   al.: SCIENCE, 275, 90 (1997).

组成

状态

容量

(A) 抗体固相化 96 孔板

洗净后使用

96   wells（8×12）／1 块

(B) GLP-1标准溶液（500 pg/mL）

稀释后使用

200 μＬ／1 瓶

(C) 缓冲液

即用

60 mL／1 瓶

(D)生物素结合抗GLP-1抗体

稀释后使用

100 μL／1 瓶

(E) 过氧化物・抗生物素蛋白结合物

稀释后使用

100 μL／1 瓶

(F) 显色液（TMB）

即用

12 mL／1 瓶

(H) 反应终止液（1M H2SO4）※小心轻放

即用

12 mL／1 瓶

( I ) 浓缩洗净液（10×）

稀释后使用

100 mL／1 瓶

封板膜

4 张

使用说明书

1 份

动物类型

对象物质

反应性和反应率（％）

Mouse/Rat

GLP-1(7-36)amide

100

GLP-1(7-37)

<   0.1

GLP-1(1-37)

―

GLP-1(9-36)amide

―

GLP-2

―

Glucagon(1-29)

―

Insulin

―

Secretin

―

GIP

―

VIP

―

GRF

―

Bovine

Glucagon(1-29)

―

VIP

―

Porcine

Glucagon(1-29)

―

VIP

―

样品

A

B

1

23.7

6.44

2

23.2

5.97

3

23.4

6.39

4

24.0

5.87

5

24.1

6.44

mean

23.7

6.22

SD

0.35

0.28

CV(%)

1.5

4.5

测量日/样品

E

F

第0天

25.1

6.31

第1天

25.1

6.16

第2天

25.0

6.24

第3天

25.0

6.37

mean

25.0

6.27

SD

0.03

0.09

CV(%)

0.13

1.4

添加量

实测值

回收量

回收率(%)

0.00

3.93

–

–

3.26

7.28

3.35

103

6.51

10.3

6.37

97.8

8.14

12.1

8.17

100

添加量

实测值

回收量

回收率(%)

0.00

11.8

–

–

7.16

19.1

7.30

102

14.3

25.5

13.7

95.8

21.5

32.4

20.6

95.8

ELISA试剂盒选择指南①②

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Fermented vegetable and fruit extract (OM-XŪ) stimulates murine gastrointestinal tract cells and RAW264. 7 cells in vitro and regulates liver gene expression in vivo. Wakame K, Nakata A, Sato K, Mihara Y, Takahata M, Miyake Y, Okada M, Shimomiya Y, and Komatsu K. Integr Mol Med, 2017 http://www.omx.co.jp/files/attachments/8e3f7824b69bece18acca14baf314b11.PDF

 6.

Gut commensal Bacteroides acidifaciens prevents obesity and improves insulin sensitivity in mice. Yang JY, Lee YS, Kim Y, Lee SH, Ryu S, Fukuda S, Hase K, Yang CS, Lim HS, Kim MS, Kim HM, Ahn SH, Kwon BE, Ko HJ, Kweon MN. Mucosal Immunol. 2017 Jan;10(1):104-116

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An extract from pork bones containing osteocalcin improves glucose metabolism in mice by oral administration. Mizokami A, Wang D, Tanaka M, Gao J, Takeuchi H, Matsui T, Hirata M. Biosci Biotechnol Biochem. 2016 Jul 27:1-8.

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Catecholamines Facilitate Fuel Expenditure and Protect Against Obesity via a Novel Network of the Gut-Brain Axis in Transcription Factor Skn-1-deficient Mice. Ushiama S, Ishimaru Y, Narukawa M, Yoshioka M, Kozuka C, Watanabe N, Tsunoda M, Osakabe N, Asakura T, Masuzaki H, Abe K. EBioMedicine. 2016 Jun;8:60-71.

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10.

Dipeptidyl peptidase-4 inhibitor, linagliptin, ameliorates endothelial dysfunction and atherogenesis in normoglycemic apolipoprotein-E deficient mice. Salim HM, Fukuda D, Higashikuni Y, Tanaka K, Hirata Y, Yagi S, Soeki T, Shimabukuro M, Sata M. Vascul Pharmacol. 2016 Apr;79:16-23.

11.

Intestinal Bile Acid Composition Modulates Prohormone Convertase 1/3 (PC1/3) Expression and Consequent GLP-1 Production in Male Mice. Morimoto K, Watanabe M, Sugizaki T, Irie J, Itoh H. Endocrinology. 2016 Mar;157(3):1071-81.

12.

Total gastrectomy-induced reductions in food intake and weight are counteracted by rikkunshito by attenuating glucagon-like peptide-1 elevation in rats. Taguchi M, Dezaki K, Koizumi M, Kurashina K, Hosoya Y, Lefor AK, Sata N, Yada T. Surgery. 2016 Jan 13. pii: S0039-6060(15)01029-6

13.

DPP-4 inhibition has beneficial effects on the heart after myocardial infarction. Akihiko Kubota, Hiroyuki Takano, Haixiu Wang, Hiroshi Hasegawa, Hiroyuki Tadokoro, Masanori Hirose, Yuka Kobaraa, Tomoko Yamada-Inagawa, Issei Komuro, Yoshio Kobayashi. Journal of Molecular and Cellular Cardiology, Volume 91, Feb. 2016, Pages 72–80

14.

The dipeptidyl peptidase IV inhibitor vildagliptin suppresses development of neuropathy in diabetic rodents: Effects on peripheral sensory nerve function, structure and molecular changes. Tsuboi K, Mizukami H, Inaba W, Baba M, Yagihashi S. J Neurochem. Volume 136, Issue 4, pages 859–870, Feb. 2016

15.

Intestinal Bile Acid Composition Modulates Prohormone Convertase 1/3 (PC1/3) Expression and Consequent GLP-1 Production in male mice. Morimoto K, Watanabe M, Sugizaki T, Irie JI, Itoh H. Endocrinology. 2016 Jan 20:en20151551.

16.

Hypoxia decreases glucagon-like peptide-1 secretion from the GLUTag cell line. Kihira Y, Burentogtokh A, Itoh M, Izawa-Ishizawa Y, Ishizawa K, Ikeda Y, Tsuchiya K, Tamaki T. Biol Pharm Bull. Vol.38(4), p514-21, 2015.

17.

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18.

Combination of DPP-4 inhibitor and PPARγ agonist exerts protective effects on pancreatic β-cells in diabetic db/db mice through the augmentation of IRS-2 expression. Hirukawa H, Kaneto H, Shimoda M, Kimura T, Okauchi S, Obata A, Kohara K, Hamamoto S, Tawaramoto K, Hashiramoto M, Kaku K. Mol Cell Endocrinol. Jun 2015.

19.

Glucagon-like peptide-1 is specifically involved in sweet taste transmission. Takai S, Yasumatsu K, Inoue M, Iwata S, Yoshida R, Shigemura N, Yanagawa Y, Drucker DJ, Margolskee RF, Ninomiya Y. FASEB J. Vol.29(6), p2268-80, Jun 2015.

20.

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21.

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22.

Duodenal-jejunal bypass improves diabetes and liver steatosis via enhanced glucagon-like peptide-1 elicited by bile acids. Kashihara H, Shimada M, Kurita N, Sato H, Yoshikawa K, Higashijima J, Chikakiyo M, Nishi M, Takasu C. J Gastroenterol Hepatol. Vol.30(2), p308-15, Feb 2015.

23.

Mosapride citrate improves nonalcoholic steatohepatitis with increased fecal lactic acid bacteria and plasma glucagon-like peptide-1 level in a rodent model. Okubo H, Nakatsu Y, Sakoda H, Kushiyama A, Fujishiro M, Fukushima T1, Matsunaga Y, Ohno H, Yoneda M, Kamata H, Shinjo T, Iwashita M, Nishimura F, Asano T. Am J Physiol Gastrointest Liver Physiol. Vol.15;308(2), G151-8, Jan 2015.

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26.

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27.

Dietary sweet potato (Ipomoea batatas L.) leaf extract attenuates hyperglycaemia by enhancing the secretion of glucagon-like peptide-1 (GLP-1) . Nagamine R, Ueno S, Tsubata M, Yamaguchi K, Takagaki K, Hira T, Hara H, Tsuda T. Food Funct, Vol.5(9), p2309-2316, Aug 2014.

28.

Duodenal-jejunal bypass improves diabetes and liver steatosis via enhanced glucagon-like peptide-1 elicited by bile acids. Kashihara H, Shimada M, Kurita N, Sato H, Yoshikawa K, Higashijima J, Chikakiyo M, Nishi M, Takasu C. Journal of Gastroenterology and Hepatology, Aug 2014.

29.

Deletion of Hypoxia-Inducible Factor-1α in Adipocytes Enhances Glucagon-Like Peptide-1 Secretion and Reduces Adipose Tissue Inflammation. Kihira Y, Miyake M, Hirata M, Hoshina Y, Kato K, Shirakawa H, Sakaue H, Yamano N, Izawa-Ishizawa Y, Ishizawa K, Ikeda Y, Tsuchiya K, Tamaki T, Tomita S. PLoS One. 2014 Apr 4;9(4):e93856

30.

Glucagon-like peptide-1 production in the GLUTag cell line is impaired by free fatty acids via endoplasmic reticulum stress. Hayashi H., Yamada R., Shankar Das S., Sato T., Takahashi A., Hiratsuka M., Hirasawa N. Metabolism – Clinical and Experimental, 2014.

31.

Dipeptidyl peptidase-4 inhibitor ameliorates early renal injury through its anti-inflammatory action in a rat model of type 1 diabetes. Kodera AR, Shikata K., Takatsuka T., Oda K., Miyamoto S., Kajitani N., Hirota D., Ono T., Usui HK., Makino H. Biochemical and Biophysical Research Communications, Vol.443(3), p828-833, Jan 2014.

32.

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33.

Cinnamtannin A2, a Tetrameric Procyanidin, Increases GLP-1 and Insulin Secretion in Mice. Yamashita Y, Okabe M, Natsume M, Ashida H. Bioscience, Biotechnology, and Biochemistry , Vol.77(4), 2013

34.

The protective roles of GLP-1R signaling in diabetic nephropathy:possible mechanism and therapeutic potential. H.Fujita, T.Morii, H.Fujishima, T.Sato, T.Shimizu, M.Hosoba, K.Tsukiyama, T.Narita, T.Takahashi, D.J.Drucker, Y.Seino, and Y.Yamada. Kidney International, 2013

35.

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36.

Vildagliptin preserves the mass and function of pancreatic β cells via the developmental regulation and suppression of oxidative and endoplasmic reticulum stress in a mouse model of diabetes. Hamamoto S, Kanda Y, Shimoda M, Tatsumi F, Kohara K, Tawaramoto K, Hashiramoto M and Kaku K. Diabetes, Obesity and Metabolism,Vol.15(2), p153-163, Feb 2013.

37.

Osteocalcin Induces Release of Glucagon-Like Peptide-1 and Thereby Stimulates Insulin Secretion in Mice. Mizokami A, Yasutake Y, Gao J, Matsuda M, Takahashi I, Takeuchi H and Hirata M. PLoS ONE 8(2): e57375. Feb 2013

38.

DPP4 inhibitor vildagliptin preserves β-cell mass through amelioration of endoplasmic reticulum stress in C/EBPB transgenic mice. Shinobu Shimizu, Tetsuya Hosooka, Tomokazu Matsuda, Shun-ichiro Asahara, Maki Koyanagi-Kimura, Ayumi Kanno, Alberto Bartolome, Hiroaki Etoh, Megumi Fuchita, Kyoko Teruyama, Hiroaki Takahashi, Hiroyuki Inoue, Yusuke Mieda, Naoko Hashimoto, Susumu Seino, and Yoshiaki Kido. J Mol Endocrinol, Vol.49, p125-135, Oct 2012.

39.

Effects of long-term treatment with the dipeptidyl peptidase-4 inhibitor vildagliptin on islet endocrine cells in non-obese type 2 diabetic Goto-Kakizaki rats. Inaba W, Mizukami H, Kamata K, Takahashi K, Tsuboi K and Yagihashi S. European Journal of Pharmacology,Vol.691(1-3), p297-306, Sep 2012.

40.

The DPP4 inhibitor linagliptin delays the onset of diabetes and preserves β-cell mass in non-obese diabetic mice. Jacob Jelsing, Niels Vrang, Soren B van Witteloostuijn, Michael Mark and Thomas Klein. J Endocrinol, Vol.214, p381-387, Sep 2012.

41.

Neural and humoral changes associated with the adjustable gastric band: insights from a rodent model. J Kampe, A Stefanidis, S H Lockie, W A Brown, J B Dixon, A Odoi, S J Spencer, J Raven and B J Oldfield. International Journal of Obesity, 27 Mar 2012.

42.

Neural and humoral changes associated with the adjustable gastric band: insights from a rodent model. J Kampe, A Stefanidis, S H Lockie, W A Brown, J B Dixon, A Odoi, S J Spencer, J Raven and B J Oldfield. International Journal of Obesity, 27 Mar 2012.

43.

Mate Tea(Ilex paraguariensis)Promotes Satiety and Body Weight Lowering in Mice:Involvement of Glucagon-Like Peptide-1. G, M, E, Hussein., H, Matsuda., S, Nakamura., M, Hamao., T, Akiyama., K, Tamura., and M, Yoshikawa. Biol.Pharm.Bull. Vol.34(12), p1849-1855, 2011.

44.

5-Hydroxy-eicosapentaenoic acid is an endogenous GPR119 agonist and enhances glucose-dependent insulin secretion. R, Kogure., K, Toyama., S, Hiyamuta., I, Kojima., S, Takeda.  Biochemical and Biophysical Research Communications Vol.416(1-2), p58-63, 2011.

45.

GLP-1 Secretion in Response to Oral and Luminal Palatinose (Isomaltulose) in Rats. T,Hira.,M,Muramatsu.,M,Okuno.and H,Hara. J Nutr Sci Vitaminol, Vol.57, p30-35, 2011.

46.

Trehalose prevents adipocyte hypertrophy and mitigates insulin resistance. C,Arai.,N,Arai.,A,Mizote.,K,Kohno.,K,Iwaki.,T,Hanaya.,S,Arai.,S,Ushio.,S,Fukuda. Nutrition Research, Vol.30(12), p840-848, 2010.

47.

Imaging exocytosis of single glucagon-like peptide-1 containing granules in a murine enteroendocrine cell line with total internal reflection fluorescent microscopy. Ohara-Imaizumi,M.,Aoyagi,K.,Akimoto,Y.,Nakamichi,Y.,Nishiwaki,C.,Kawakami,H.and Nagamatsu,S. Biochemical and Biophysical Reseach Communications, Vol.390, p16-20, 2009.

小胶质细胞标记抗体（免疫染色用）

产品编号

产品名称【中文名称】

规格

包装

019-19741

Anti Iba1, Rabbit (for   Immunocytochemistry)

【抗Iba1，兔（免疫细胞化学）】

免疫化学用

50 μg

抗体信息

抗原名

Iba1

适用

实验

免疫

染色

同种型

IgG

免疫染色图像

抗原信息

Iba1C末端序列肽

物种交叉反应性

人，小鼠，大鼠

标签

非标签

抗原别名

AIF-1, IRT1, Protein G1

免疫

动物

兔

克隆号

–

（多克隆抗体）

详细信息

Iba1是与巨噬细胞/小胶质细胞特异性表达，分子量17000的钙结合蛋白。近年来，小胶质细胞除了对神经营养·保护作用以外，产生的NO、TNF- α、IL-1 β对神经伤害作用很受关注。

本产品是与小胶质细胞特异性反应的兔多克隆抗体，适用于与星形胶质细胞特异性GFAP单克隆抗体的双重染色。

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能并放2块孔板，可在工作台内边保温边作业。

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