Blotting实验专用

制造商：FUJIFILM Wako Pure Chemical Corporation

制造商：（富士胶片和光）

储存条件：室温

低背景！

1.　1 w/v% Skim milk in TBS-T

2.　5 w/v% BSA(Albumin, from Bovine Serum) in TBS-T

上样量：10 ug of cell lysate/Lane

抗体：Anti β-actin antibody

低背景！

1.　5w/v% Skim milk in TBS-T

2.　Company A Protein-free Blocking Buffer

样品：DYKDDDDK-BAP

Lane#1： 10 ng

Lane#2： 5 ng

Lane#3： 2.5 ng

Antibody : Anti DYKDDDDK antiboby

试剂

包装

ProteoStar™ Aggresome Detection Reagent

10 μL

Hoechst 33342 Nuclear Stain

50 μL

Proteasome Inhibitor (MG-132)

120 nM

10X Assay Buffer

25 mL

用0.2% DMSO模拟培育Jurkat细胞或在37℃下用 5 µM的MG-132过夜培育。用荧光素p62 Ab (1:500稀释原液)培育细胞。清洗细胞后，通过流式细胞仪在FL1信道使用488 nm的激光进行分析。在MG132处理的细胞时，荧光素p62 Ab的抗体信号增加约2.5倍。

　　用5 μM MG-132培育带有HeLa细胞的聚集小体12小时后（右图），加入ProteoStat^® 聚集小体染料（红色）和用Hoechst33342复染，然后进行观察。未经培育的聚集小体（左图）。

　　预先用5μMMG-132培育带有HeLa细胞的聚集小体12小时后，用ProteoStat^® 聚集小体染料来染色观察（左上图绿色部分）；与AlexaFluor^® 647微管蛋白抗体（左下图红色部分）共定位；通过荧光显微镜观察的合成图像（右图）。

　　α-syn混合物形式聚集在被内化到M17神经母细胞瘤细胞系的细胞质膜。用α-syn单体处理M17细胞，声波处理α-SIGN PFFs或α-SYN混合物（M + F）。四天后，在固定M17细胞前，将其清洗3次，用 PROTEOSTAT^® 聚合染料（绿色）染色和用抗α-syn（红）和β连环蛋白（灰色）免疫染色。比例尺= 20 µm。作者提到“Fibril生长和播种量α-synuclein蛋白介导的细胞凋亡中发挥重要作用。”此图来自A-L Mahul-Mellier, et al.; Cell Death &

Differentiation (2015). (doi:10.1038/cdd.2015.79).

在PROTEOSTAT^® 聚集小体检测试剂（A）和Hoechst 33342核染色（B）的激发和发射光谱。

　　由内质网应激的自噬细胞产生的聚集小体和聚集小体类的包涵体，epi-荧光显微成像图。未经处理的K562细胞（A）；加入5 mM MG-132溶液培育24小时（B）；加入50 mM CQ培育24小时（C）；加入100 nM毒胡萝卜素（D）。在温室下，用PROTEOSTAT^® 染料(1:10,000稀释)来固定染色细胞30分钟。PROTEOSTAT^® 的着色显示 聚集体 和 ALIS呈红色， DAPI 呈蓝色。（Courtesy of the Flow Cytometry Core Facility, Blizard Institute,

Queen Mary University of London, London, UK）

　　分别25，50，75 mmCQ，100 nM的毒胡萝卜素（TG）或5mM的MG-132处理K562细胞24小时。然后在温室下用PROTEOSTAT^® 染料（1：10,000稀释）固定并透化培育细胞30分钟。接着在BD LSR II的蓝色610/20 nm的信道分析细胞（30,000）。Courtesy of the Flow Cytometry Core Facility, Blizard Institute, Queen Mary

University of London, London, UK.

　　分别使用毒胡萝卜素（Tg，0.1微米），25，50和75微米氯喹（CQ）处理K562 细胞24小时，来诱导内质网应激和不完全自噬细胞。采用阳性对照，加入蛋白酶体抑制剂，镁 132 (5 µ M)培育 24小时。固定并透化沉淀的细胞。接着根据说明书的指示，在室温下用 300 µL PROTEOSTAT^® 聚集小体检测试剂盒 （Enzo Life Sciences）培育细胞30分钟，加入1 微克/毫升 DAPI （用来测定细胞周期）。S值聚集小体小倾向的因子（APF）相对增加，G1和G2m的S值是通过每次细胞处理后检测的结果和对照组相比而得出。S期和G1相比，ER 应激诱导剂、Tg和 50 µM CQ的聚集小体数量呈上升趋势，同时显示蛋白酶体抑制剂，MG-132和50μMCQ下降的聚集小体数量在G2M S期比对照水平更低。Courtesy of the Flow Cytometry Core Facility, Blizard Institute, Queen Mary University of London, London, UK.

【1】 A fast and specific method to screen for intracellular amyloid inhibitors using bacterial model systems: S. Navarro, et al.; Eur. J. Med. Chem. (2015), Application(s): Confocal microscopy, Abstract;

【2】 Amyloidogenic lysozymes accumulate in the endoplasmic reticulum accompanied by the augmentation of ER stress signals: Y. Kamada, et al.; Biochim. Biophys. Acta 1850, 1107 (2015), Application(s): Microscopy, Abstract;

Conophylline protects cells in cellular models of neurodegenerative diseases by inducing mammalian target of rapamycin (mTOR)-independent autophagy: Y. Sasazawa, et al.; J. Biol. Chem. 290, 6168 (2015), Abstract;

【3】 Decreased proteasomal function accelerates cigarette smoke-induced pulmonary emphysema in mice: Y. Yamada, et al.; Lab. Invest. 95, 625 (2015), Application(s):Aggresome detection by fluorescence microscopy in fibroblasts, Abstract;

【4】Defective autophagy is a key feature of cerebral cavernous malformations: S. Marchi, et al.; EMBO Mol. Med. 7, 1403 (2015), Application(s): Aggresome detection in aggregated proteins and aggresome‐like inclusion bodies in fixed and permeabilized samples,Abstract; Full Text

【5】 Fibril growth and seeding capacity play key roles in α-synuclein-mediated apoptotic cell death: A.L. Mahul-Mellier, et al.; Cell Death Differ. 22, 2107 (2015), Abstract;

【6】 In vitro administration of gold nanoparticles functionalized with MUC-1 protein fragment generates anticancer vaccine response via macrophage activation and polarization mechanism: T. Mocan, et al.; J. Cancer 6, 583 (2015), Application(s): Aggresome detection by fluorescence microscopy in peritoneal macrophages, Abstract; Full Text

【7】 Intensified autophagy compromises the efficacy of radiotherapy against prostate cancer: M.I. Koukourakis, et al.; Biochem. Biophys. Res. Commun. 461, 268 (2015),Application(s): Fluorescence microscopy , Abstract;

Mevalonate pathway regulates cell size homeostasis and proteostasis through autophagy: T.P. Miettinen, et al.; Cell Rep. 13, 2610 (2015), Application(s): Flow cytometry analysis of protein aggregation using Jurkat, U2OS, Kc167 and HUVEC cells, Abstract;

【8】 MiR-29b replacement inhibits proteasomes and disrupts aggresome+autophagosome formation to enhance the antimyeloma benefit of bortezomib: S. Jagannathan, et al.; Leukemia 29, 727 (2015), Application(s): Detection of protein aggregates by fluorescence microscopy in multiple myeloma cell lines, Abstract; Full Text

【9】 Molecular chaperone GRP78 enhances aggresome delivery to autophagosomes to promote drug resistance in multiple myeloma: M.A. Abdel Malek, et al.; Oncotarget 6, 3098 (2015), Application(s): Confocal Microscopy, Abstract; Full Text

【10】 Monitoring of dipeptidyl peptidase-IV (DPP-IV) activity in patients with mucopolysaccharidoses types I and II on enzyme replacement therapy – Results of a pilot study: K. Hetmanczyk, et al.; Clin. Biochem. (2015), Application(s): Plasma DPP-IV enzyme assay, Abstract;

【11】 Pressure overload-induced cardiac dysfunction in aged male adiponectin knockout mice is associated with autophagy deficiency: J.W. Jahng, et al.; Endocrinology 156, 1667 (2015),Abstract;

Protein kinase C-dependent growth-associated protein 43 phosphorylation regulates gephyrin aggregation at developing GABAergic synapses: C.Y. Wang, et al.; Mol. Cell. Biol.35, 1712 (2015), Abstract;

Schwann cells contribute to neurodegeneration in transthyretin amyloidosis: T. Murakami, et al.; J. Neurochem. 134, 66 (2015), Abstract;

【12】 Cationic polystyrene nanospheres induce autophagic cell death through the induction of endoplasmic reticulum stress: H.W. Chiu, et al.; Nanoscale 7, 736 (2014), Abstract;

Direct visualization of HIV-enhancing endogenous amyloid fibrils in human semen: S.M. Usmani, et al.; Nat. Commun. 5, 3508 (2014), Application: Amyloid detection in semen,Abstract;

【13】 Distinct patterns of HSP30 and HSP70 degradation in Xenopus laevis A6 cells recovering from thermal stress: S. Khan, et al.; Comp. Biochem. Physiol. A Mol. Integr. Physiol. 168, 1 (2014), Application(s): Detection of aggresomes in Xenopus laevis cells using fluorescence microscopy, Abstract;

Dynein function and protein clearance changes in tumor cells induced by a kunitz-type molecule, amblyomin-x: M.T. Pacheco, et al.; PLoS One 9, e111907 (2014), Application(s):Detection of aggresomes by flow cytometry, Abstract; Full Text

【14】 Higher vulnerability and stress sensitivity of neuronal precursor cells carrying an alpha-synuclein gene triplication: A. Flierl, et al.; PLoS One 9, e112413 (2014), Application(s):Detection of protein aggregates by fluorescence microscopy and flow cytometry in neuronal precursor cells, Abstract; Full Text

【15】Human stefin B role in cell's response to misfolded proteins and autophagy: M. Polajnar, et al.; PLoS One 9, e102500 (2014), Application(s): Detection of protein aggregates in primary astrocytes, Abstract; Full Text

【16】 Novel estradiol analogue induces apoptosis and autophagy in esophageal carcinoma cells: E. Wolmarans, et al.; Cell Mol. Biol. Lett. 19, 98 (2014), Abstract;

【17】 Preconditioning stimulus of proteasome inhibitor enhances aggresome formation and autophagy in differentiated SH-SY5Y cells: Y. Bang, et al.; Neurosci. Lett. 566, 263 (2014),Abstract;

【18】 Protein deubiquitination during oocyte maturation influences sperm function during fertilisation, antipolyspermy defense and embryo development: Y.J. Yi, et al.; Reprod. Fertil. Dev. (2014), Application(s): Detection of protein aggregates in oocytes, Abstract;

【19】 Protein expression pattern of PAWP in bull spermatozoa is associated with sperm quality and fertility following artificial insemination: C.E. Kennedy, et al.; Mol. Reprod. Dev. 81, 436 (2014), Abstract;

Serine/threonine kinase 16 and MAL2 regulate constitutive secretion of soluble cargo in hepatic cells: J.G. In, et al.; Biochem. J. 463, 201 (2014), Abstract;

【20】SGTA regulates the cytosolic quality control of hydrophobic substrates: L. Wunderley, et al.; J. Cell. Sci. 127, 4728 (2014), Application(s): Dual staining with ProteoStat® dye, Abstract;Full Text

【21】 T he small heat shock protein B8 (HSPB8) confers resistance to bortezomib by promoting autophagic removal of misfolded proteins in multiple myeloma cells: M. Hamouda, et al.; Oncotarget 5, 6252 (2014), Application(s): Analysis of velcade resistant multiple myeloma human cells by WB, Assay, Abstract; Full Text

【22】Aldosterone and angiotensin II induce protein aggregation in renal proximal tubules: M.U. Cheema, et al.; Physiol. Rep. 1, e00064 (2013), Application(s): Labeling of kidney homogenates, labeled particles sorted by flow cytometry and identification by LC-MS/MS ,Abstract; Full Text

【23】Covalent and allosteric inhibitors of the ATPase VCP/p97 induce cancer cell death: P. Magnaghi, et al.; Nat. Chem. Biol. 9, 548 (2013), Application(s): Detection of aggresomes in human colon carcinoma HCT116 cells using fluorescence microscopy, Abstract;

【24】 Environmental stresses induce misfolded protein aggregation in plant cells in a microtubule-dependent manner: Y. Nakajima, et al.; Int. J. Mol. Sci. 14, 7771 (2013),Application(s): Detection of aggresomes using fluorescence microscopy, Abstract; Full Text

【25】 In vitro changes in mitochondrial potential, aggresome formation and caspase activity by a novel 17-β-estradiol analogue in breast adenocarcinoma cells: D.S. Nkandeu, et al.; Cell. Biochem. Funct. 31, 566 (2013), Abstract;

Increased generation of cyclopentenone prostaglandins after brain ischemia and their role in aggregation of ubiquitinated proteins in neurons: H. Liu, et al.; Neurotox. Res. 24, 191 (2013), Abstract;

【26】 Macrolide antibiotics block autophagy flux and sensitize to bortezomib via endoplasmic reticulum stress-mediated CHOP induction in myeloma cells: S. Moriya, et al.; Int. J. Oncol.42, 1541 (2013), Application(s): Detection of aggresomes using flow cytometry, Abstract;Full Text

【27】Mst1 inhibits autophagy by promoting the interaction between Beclin1 and Bcl-2: Y. Maejima, et al.; Nat. Med. 19, 1478 (2013), Application(s): Detection of aggresomes in mouse heart sections using fluorescence microscopy, Abstract; Full Text

【28】 N-terminally truncated forms of human cathepsin F accumulate in aggresome-like inclusions: B. Jeric, et al.; Biochim. Biophys. Acta 1833, 2254 (2013), Application(s):Detection of aggresomes using fluorescence microscopy, Abstract;

【29】 The ubiquitin proteasome system regulates the stability and activity of the glucose sensor glucokinase in pancreatic beta cells: A. Hofmeister-Brix, et al.; Biochem. J. 456, 173 (2013),Abstract; Full Text

【30】 VCP Phosphorylation-Dependent Interaction Partners Prevent Apoptosis in Helicobacter pylori-Infected Gastric Epithelial Cells: C.C. Yu, et al.; PLoS One 8, e55724 (2013),Application(s): Aggresome detection in AGS human gastric epithelial cells, Abstract; Full Text

【31】 Zerumbone, an electrophilic sesquiterpene, induces cellular proteo-stress leading to activation of ubiquitin-proteasome system and autophagy: K. Ohnishi, et al.; BBRC 430, 616 (2013), Application(s): Aggresome detection in mouse hepatocytes, Abstract;

【32】Autophagy in idiopathic pulmonary fibrosis: A.S. Patel, et al.; PLoS One 7, e41394 (2012),Application(s): Detection of aggresomes in lung tissue sections using fluorescence microscopy, Abstract; Full Text

【33】 Decreased proteasomal activity causes age-related phenotypes and promotes the development of metabolic abnormalities: U. Tomaru, et al.; Am. J. Pathol. 180, 963 (2012),Abstract;

【34】 Mutations in the area composita protein αT-catenin are associated with arrhythmogenic right ventricular cardiomyopathy: J. van Hengel, et al.; Eur. Heart J. 34, 201 (2012),Abstract;

【35】 Quantitative analysis of α-synuclein solubility in living cells using split GFP complementation: A. Kothawala, et al.; PLoS One 7, e43505 (2012), Application(s):Aggresome detection in HeLa cells, Abstract; Full Text

【36】 Multiple aggregates and aggresomes of C-terminal truncated human αA-crystallins in mammalian cells and protection by αB-crystallin: I. Raju, et al.; PLoS One 6, e19876 (2011), Application(s): Aggresome detection in HeLa cells, Abstract; Full Text

【37】 Novel Cell- and Tissue-Based Assays for Detecting Misfolded and Aggregated Protein Accumulation Within Aggresomes and Inclusion Bodies: D. Shen, et al.; Cell Biochem. Biophys. 60, 173 (2011), Abstract; Full Text

【38】Inhibitors of protein aggregation and toxicity: H. Amijee, et al.; Biochem. Soc. Trans. 37, 692 (2009), Abstract; Full Text

【39】Autophagy-mediated clearance of aggresomes is not a universal phenomenon: E. Wong, et al.; Hum. Mol. Genet. 17, 2570 (2008), Abstract; Full Text

^{【40】Chemical and biological approaches synergize to ameliorate protein-folding diseases: T.W. Mu, et al. ; Cell 134, 769 (2008), Abstract; Full Text}

【41】Inhibitors of the proteasome suppress homologous DNA recombination in mammalian cells: Y. Murakawa, et al.; Cancer Res. 67, 8536 (2007), Abstract;

【42】 p62SQSTM1 forms protein aggregates degraded by autophagy and has a protective effect on huntingtin-induced cell death: G. Bjørkøy, et al.; J.Cell Biol. 171, 603 (2005), Full Text

【43】Therapeutic effects of cystamine in a murine model of Huntington's disease: A. Dedeoglu, et al.; J. Neurosci. 22, 8942 (2002), Full Text

组成品

状态

包装

（A）抗体包被96孔板

清洗后使用

96 wells（8×12）／1个

（B）标准溶液（100 ng/mL）

稀释后使用

200 μL/1瓶

（C）测定用缓冲液

直接使用

60 mL/1瓶

（D）生物素结合抗GH抗体

稀释后使用

100 μL/1瓶

（E）过氧化物酶·抗生素结合物

稀释后使用

100 μL/1瓶

（F）显色液（TMB）

直接使用

12 mL/1瓶

（G）样本稀释用缓冲液

直接使用

12 mL/1瓶

（H）终止液（1M H₂SO₄）

※小心轻放

直接使用

12 mL/1瓶

（I）浓缩清洗液（10×）

稀释后使用

100 mL/1瓶

孔板密封膜

4个

产品说明书

1本

样本

A

B

1

8.74

1.15

2

8.78

1.03

3

8.79

1.07

4

8.68

1.06

5

8.03

1.03

6

8.27

1.13

7

7.94

1.07

8

7.97

1.05

Mean

8.40

1.07

SD

0.39

0.04

CV（%）

4.6

3.9

测定日/样本

E

F

G

0天

5.01

1.25

0.328

1天

5.01

1.24

0.329

2天

5.00

1.27

0.347

3天

5.00

1.28

0.337

Mean

5.00

1.26

0.335

SD

0.0019

0.016

0.0088

CV（%）

0.038

1.3

2.6

添加量

实测值

回收量

回收率（%）

0.00

1.29

—

—

0.584

1.89

0.601

103

1.17

2.48

1.19

102

1.75

3.01

1.73

98.5

添加量

实测值

回收量

回收率（%）

0.00

2.23

—

—

1.36

3.59

1.35

100

3.39

5.60

3.36

99.2

4.07

6.21

3.97

97.7

 1.

Easy detection of hormone secretion from LβT2 cells by using Gaussia luciferase.
Satou K, Mochimaru Y, Nakakura T, Kusada T, Negishi J, Musha S, Yoshimura N, Kato Y, Tomura H.
J Reprod Dev. 2017 Apr 21;63(2):199-204.

 2.

α-Lipoic acid potentially targets AMP-activated protein kinase and energy production in the fetal brain to ameliorate dioxin-produced attenuation in fetal steroidogenesis.
Takeda T, Matsuo Y, Nishida K, Fujiki A, Hattori Y, Koga T, Ishii Y, Yamada H.
J Toxicol Sci. 2017;42(1):13-23.

 3.

Kisspeptin expression is decreased in the arcuate nucleus of hypothyroid female rats with irregular estrus cycles.
Tomori Y, Takumi K, Iijima N, Takai S, Ozawa H.
Neurosci Res. 2016 Nov 27.

 4.

Increase of kisspeptin-positive cells in the hypothalamus of a rat model of polycystic ovary syndrome. Kondo M, Osuka S, Iwase A, Nakahara T, Saito A, Bayasula, Nakamura T, Goto M, Kotani T, Kikkawa F

Metab Brain Dis. 2016 Jun;31(3):673-81.

 5.

The combined fixed-dose antituberculous drugs alter some reproductive functions with oxidative stress involvement in wistar rats O. Awodele, B.Pharm M.Sc MPH PhD D.Sc FPCPharm FASIa, A.A. Momoha, N.A. Awololac, O.E. Kalea, b, W.O. Okunowod

Toxicology Reports,Vol.3, 2016, Pages 620–627

 6.

Hormone Inhibition During Mini-Puberty and Testicular Function in Male Rats.
Chen L, Wang R, Wang W, Lu W, Xiao Y, Wang D, Dong Z.
Int J Endocrinol Metab. 2015 Oct 3;13(4):e25465

 7.

Increase of kisspeptin-positive cells in the hypothalamus of a rat model of polycystic ovary syndrome.
Kondo M, Osuka S, Iwase A, Nakahara T, Saito A, Bayasula, Nakamura T, Goto M, Kotani T, Kikkawa F
Metab Brain Dis. 2016 Feb 11. [Epub ahead of print

 8.

Oleuropein supplementation increases urinary noradrenaline and testicular testosterone levels and decreases plasma corticosterone level in rats fed high-protein diet
Oi-Kano Y., Kawada T., Watanabe T., Koyama F., Watanabe K., Senbongi R., Iwai K.
The Journal of Nutritional Biochemistry, Vol.24(5), p887-893, May 2013.

 9.

Multiple Cholinergic Signaling Pathways in Pituitary Gonadotrophs

Zemkova H., Kucka M., Bjelobaba IV., Tomi M. and Stojilkovic SS.
Endocrinology, Vol.154(1), p421-433, Jan 2013.

10.

ERα/E2 signaling suppresses the expression of steroidogenic enzyme genes via cross-talk with orphan nuclear receptor Nur77 in the testes
S.-Y. Lee., E. Park., S.-C. Kim., R.-S. Ahn., C. Ko., K. Lee.
Molecular and Cellular Endocrinology, 2012.

11.

Gonadotropin-Inhibitory Hormone Inhibits GnRH-Induced Gonadotropin Subunit Gene Transcriptions by Inhibiting AC/cAMP/PKA-Dependent ERK Pathway in LβT2 Cells
Son YL., Ubuka T., Millar RP., Kanasaki H. and Tsutsui K.
Endocrinology, Vol.153(5), p2332-2343, May 2012.

12.

Anti-androgenic effects of S-40542, a novel non-steroidal selective androgen receptor modulator (SARM) for the treatment of benign prostatic hyperplasia
H. Nejishima., N. Yamamoto., M. Suzuki., K. Furuya., N. Nagata., S. Yamada.
The Prostate published online:16 MAR 2012

13.

Gonadotropin-Inhibitory Hormone Inhibits GnRH-Induced Gonadotropin Subunit Gene Transcriptions by Inhibiting AC/cAMP/PKA-Dependent ERK Pathway in LβT2 Cells
Y. L. Son., T. Ubuka., R. P. Millar., H. Kanasaki., and K. Tsutsui.
Endocrinology, Feb 28, 2012

14.

Neonatal immune challenge alters reproductive development in the female rat
L. Sominsky., C.L. Meehan., A.K. Walker., L. Bobrovskaya., E. A. McLaughlin., D. M. Hodgson.
Hormones and Behavior, 2012.

孤儿配体Apelin水平中和抗体

产品编号

产品名称【中文名称】

规格

包装

013-25871 

Anti Apelin, Monoclonal Antibody (4G5)

【抗Apelin，单克隆抗体（4G5）】

免疫化学用

100μL

抗体信息

抗原名

Apelin

适用实验

中和实验，免疫染色、ELISA

同种型

IgG₁

—

抗原信息

pGlu65-Apelin13
  (QRPRLSHKGPMPF)

物种交叉反应性

人，小鼠，大鼠

标签

非标签

抗原别名

APLN, APEL, XNPEP2, AGTRL1 Ligand

免疫动物

小鼠

克隆号

4G5

详细信息

Apelin是由36个氨基酸组成的生理活性肽配体。与APJ受体结合从而激活APJ受体。显示血压下降作用，血管重生作用，动脉硬化作用。Apelin也存在于神经系统。近年来有报道称用于ALS小鼠模型神经保护作用。

本产品是针对Apelin相关的抗体。结合Apelin的活性部位表现中和活性。

使用文献

1. Kidoya, H. et al.: EMBO. J.,   27, 522 (2008).
  2. Kawamata, Y. et al.: Biochimica. et. Biophysica. Acta.,1538, 162 (2001).

包装

可使用次数（Wells）

6 wells

24 wells

48 wells

96 wells

1 set

16

65

130

325

4 set

65

260

520

1,300

16 set

260

1,040

2,080

5,200

产品编号

厂商编号

产品名称

包装

384-15261

GG001

GenomONE^®–GE

1 set

380-15263

GG004

4 set

388-15264

GG016

16 set

产品编号

产品名称

制造商

包装

319-08641

Cas9 Nuclease protein NLS（3 μg/μL）

NIPPON GENE

75 μg

316-08651

Cas9 Nuclease protein NLS（15 μg/μL）

NIPPON GENE

300 μg

产品编号

产品名称

制造商

包装

314-08691

CUGA7 gRNA Synthesis Kit
CUGA7 gRNA合成试剂盒

NIPPON GENE

50次用

产品编号

产品名称

制造商

包装

313-08801

T7 Endonuclease I reaction Mix

NIPPON GENE

50 μL

产品编号

厂商编号

产品名称

用途

包装

388-15281

GS001

GenomONE^®–Si

in vitro中siRNA/miRNA
转染试剂盒

1 set

384-15283

GS004

4 set

382-15284

GS016

16 set

388-15286

GS040

40 set

385-15291

GX001

GenomONE^®–GX

in vitro中质粒DNA转染试剂盒

1 set

381-15293

GX004

4 set

389-15294

GX016

16 set

385-15296

GX040

40 set

381-15271

GN01F

GenomONE^®–Neo（FD）

in vivo 中质粒DNA、

siRNA/miRNA 、蛋白转染试剂盒

1 set

387-15273

GN04F

4 set

385-15274

GN16F

16 set

381-15276

GN40F

40 set

387-15251

CF001

GenomONE^®–CF

细胞融合试剂盒（杂交瘤的制备，核转移至脱核未受精卵）

1 set

383-15253

CF004

4 set

381-15254

CF016

16 set

产品编号

产品名称

目数

规格

厚度（μm）

纤维直径（μmΦ）

WEB26453

PFA网片

25

25目（1 m×10 m)

500

250

产品编号

产品名称

目数

规格

厚度（μm）

纤维直径（μmΦ）

WEB26454

PFA网片

80

80目（1 m×10 m)

160

80

WEB26455

PFA网片

100

100目（1 m×10 m)

100

50

中文名

英文名

缩写

符号

等电点

精氨酸

Arginine

Arg

R

10.76

赖氨酸

Lysine

Lys

K

9.74

组氨酸

Histidine

His

H

7.59

Peak#

R.T.

M.W.

Sequence

Peak 1

7.06 min

950.15

YGKRLWK

Peak 2

8.27 min

821.98

YGKRLW

产品编号

产品名称

规格

比活

RCPB0302

RCPB

10 mg

150 U/mg

RCPB0304

RCPB

10 μg

150 U/mg

产品编号

产品名称

规格

储存条件

其他信息

MDRK0301

RCPB ELISA Kit
重组羧肽酶B检测试剂盒

96 孔

2～8 ℃

检测范围：
15.6～1000 ng/mL