确认材料的耐药性 >> 耐药性检索
日度归档:2024年3月9日
Millipore分析型不锈钢换膜过滤器XX3001240
Millipore分析型不锈钢换膜过滤器XX3001240
说明:分析型换膜过滤器 13 mm,不锈钢
数量/包装:1
进口接头:漏斗
直径,cm (in):3.8
高度,cm (in):8.9
出口接头:阳 luer 滑动接头
结构材质:不锈钢
滤膜直径,mm:13
密封材质:PTFE
产品名称:不锈钢换膜过滤器
过滤面积,cm2:0.7
漏斗容量:40ml
Whatman1093-935Grade 93湿强定性滤纸 GR 93 61x61CM 500/PK
【简单介绍】
【简单介绍】
【详细说明】
上海金畔生物科技有限公司
文章号20478286-20478286
PFA圆棒 外径(φmm):30 长度(mm):300日本三博特sanplatec
确认材料的耐药性 >> 耐药性检索
PALL尼龙膜NHG047100孔径0.8um
PALL尼龙膜NHG047100孔径0.8um
PALL ULTIPORN66,Part No:NHG047100,Removal Rating:0.8um
PALL尼龙膜清洁度检测滤膜0.8um孔径NHG047100
NHG047100:47mm直径,0.8um孔径,100PK
PALL尼龙膜汽车零配件清洁度检测滤膜0.8um孔径NHG025100
NHG025100:25mm直径,0.8um孔径,100PK
Whatman10463877无粘合剂玻璃微纤维滤纸UNIFLO 13/0.2 NYL 500/PK
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上海金畔生物科技有限公司
文章号:20823840-20823840
日本同仁化学Hampton蛋白结晶试剂盒Trimethylamine N-oxide dihydrate/HR2-777- DOJINDO
上海金畔生物科技有限公司代理日本同仁化学 DOJINDO代理商全线产品,欢迎访问官网了解更多信息
上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等,我们将竭诚为您服务,欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。
Products > Optimize Reagents > Optimize – Salts > Trimethylamine N-oxide dihydrate
Trimethylamine N-oxide dihydrate
CAT NO
HR2-777
NAME
DESCRIPTION
100 mL
PRICE
$130.00
支持材料
应用
- Crystallization grade Trimethylamine N-oxide dihydrate for formulating screens or for optimization
- 结晶级三甲胺 N-氧化物二水合物,用于配制筛选或优化
特征
- Sterile filtered solution
- Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
- 无菌过滤溶液
在 1+ 型超纯水中配制:25°C 时电阻率为 18.2 兆欧厘米,总有机碳 < 5 ppb,无细菌(<1 细菌 (CFU/ml)),无热原(<0.03 内毒素 (EU/ml)) , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)
描述Trimethylamine N-oxide dihydrate
Synonym: TMANO
(CH3)3NO • 2H2O
C3H9NO • 2H2O
Mr 111.14
CAS Number [62637-93-8]
EC Number 214-675-6
Beilstein Registry Number 4(4)144
Merck 14,9711
RTECS YH2850000
MDL Number MFCD00002048
Purity > 98.0%
Maximum solubility is 6.0 M at 25°C (tested by Hampton Research)
Measured pH Range: 8.0 – 9.3 at 25°C
Measured Conductivity Range: 10.9 – 67.1 µS/cm at 25°C
Measured Refractive Index Range: 1.34320 – 1.34348 at 20°C
同义词: TMANO
(CH3)3NO • 2H2O
C3H9NO • 2H2O
111.14 先生
CAS 编号 [62637-93-8]
欧盟编号 214-675-6
贝尔斯坦注册号 4(4)144
默克 14,9711
RTECS YH2850000
MDL 编号 MFCD00002048
纯度 > 98.0%
25°C 时最大溶解度为 6.0 M(由 Hampton Research 测试)
测得的 pH 范围:25°C 时 8.0 – 9.3
测得的电导率范围:25°C 时为 10.9 – 67.1 µS/cm
测得的折射率范围:20°C 时为 1.34320 – 1.34348
Trimethylamine N-oxide dihydrate
参考
1. Use of organic cosmotropic solutes to crystallize flexible proteins: Application to T7 RNA polymerase and its complex with the inhibitor T7 lysozyme. Jeruzalmi and Steitz, J. Mol. Bio. (1997) 274, 748-756.
2. Trimethylamine N-oxide as a versatile cryoprotective agent in macromolecular crystallography. Mueller-Dieckmann, C., Kauffmann, B., and Weiss, M. J. Appl. Cryst. (2011). 44, 433–436.
3. Crystallization of a functionally intact Hsc70 chaperone. J. Jiang, E. M. Lafer and R. Sousa. Acta Cryst. (2006). F62, 39-43.
4. Doolittle, R. F. (2003). Biophys. Chem. 100, 307–313.
5. Hill, M. C., Bates, I. R., White, G. F., Hallett, F. R. & Harauz, G. (2002). J.
6. Auton, M. & Bolen, D. W. (2005). Proc. Natl Acad. Sci. USA, 102, 15065–15068.
7. Bolen, D. W. (2004). Methods, 34, 312–322.
异径管弯头(30系列)(简称;30-13RUE6)日本三博特sanplatec
确认材料的耐药性 >> 耐药性检索
A |
B |
C |
D |
Cx |
Dx |
E |
F |
Fx |
W |
T (mm) |
适用管外径(inch) |
Tx (mm) |
适用管外径(inch) |
产品编号 |
简称 |
30 |
25 |
25 |
18 |
14 |
10 |
4 |
26 |
12 |
22 |
13 |
– |
6 |
1/4 |
12276 |
30-13RUE6 |
单位:mm
主体:PTFE
螺帽:ECTFE
W是对角线的尺寸。
特点
毫米、英寸规格的管均可通用。
最大使用压力请咨询。
Whatman10300114Grade 589/2定量滤纸 589/2 WHTRIB 185MM 100/PK
【简单介绍】
【简单介绍】
【详细说明】
上海金畔生物科技有限公司
文章号:20656245-20656245
日本JaICA 代理Hexanoyl-Lysine adduct(HEL) ELISA Kit
日本JaICA 代理Hexanoyl-Lysine adduct(HEL) ELISA Kit
Suitable for assessment of oxidative stress using urine, serum and cultured cells. For research use only.
已酰-赖氨酸(HEL)ELISA试剂盒,适用于尿液,血清和细胞培养样品氧化应激的评估。
仅供研究使用。
什么是己酰-赖氨酸?
Biomarker for early stage of lipid oxidation
Oxidative damage of lipids caused by reactive oxygen species (ROS) play an important role in some diseases, lesion of cell functions and aging. Aldehydes such as malondi-aldehyde (MDA) and 4-hydroxy-2-nonenal (4-HNE) have been reported as one of the advanced lipid peroxidation products. But recently in the earlier stage of lipid peroxidation, 13-hydroperoxyoctadecanoic acid (13-HPODE) is found to be covalently bound to proteins1). Hexanoyl-Lysine adduct (HEL) is a novel lipid hydroperoxide-modified lysine residues. HEL is formed by oxidative modification by oxidized omega-6 fatty acids such as linoleic acid or arachidonic acid. HEL may be a useful biomarker for initial stage of lipid peroxidation.Monoclonal antibodies and ELISA kit have been developped, and HEL can be detected in oxidatively modified LDL, in human atherosclerotic lesions, human urine and serum. It is also reported that HEL is formed in rat muscle during exercise, and the formation is prohibited by antioxidants such as flavonoids.
由活性氧(ROS)引起的脂质氧化损伤在一些疾病、细胞功能损害和衰老中起重要作用。醛类如丙二醛(MDA)和4-羟基-2-壬烯醛(4-HNE)已被报道为先进的脂质过氧化产物之一。但最近在脂质过氧化的早期阶段,发现13-氢过氧十八烷酸(13-HPODE)与蛋白质共价结合。己酰-赖氨酸加合物(HEL)是一种新的脂质氢过氧化物修饰的赖氨酸残基。HEL是通过氧化的omega-6脂肪酸(例如亚油酸或花生四烯酸)的氧化修饰形成。HEL可能是脂质过氧化初始阶段有用的生物标志物。现在我们已经开发了HEL单克隆抗体和ELISA试剂盒,并且可以在氧化修饰的低密度脂蛋白(LDL)、人动脉粥样硬化病变、人尿液和血清中检测HEL。另据报道,大鼠肌肉在运动期间形成HEL,并且通过抗氧化剂(如类黄酮)是禁止形成的。
己酰-赖氨酸(HEL)ELISA试剂盒
JaICA have developed HEL ELISA kit in collaboration with Dr. Toshihiko Osawa (Nagoya University) and Dr. Yoji Kato (Univerisity of Hyogo). This ELISA kit can be applied to urine, serum and cultured cells form human and animal.
JaICA公司与Toshihiko Osawa博士(名古屋大学)和Yoji Kato博士(兵库大学)合作开发了Hexanoyl-Lys(HEL)ELISA试剂盒。
该ELISA试剂盒可应用于人和动物的尿液、血清和细胞培养。
Assay principle: | Competitive ELISA (detection: 450 nm) |
Specifity: | Specific to N-epsilon-Hexanoyl-Lysine adduct |
Measuring range: | 2 – 700 nmol/L |
Time to answer: | Over night and 2 hours. |
Format: | 96 wells (54 samples in single assay) |
Applications: | Urine, serum and cultured cells from human and animals. |
Storage: | Store at 2 – 8掳C (don’t freeze). |
Expiry: | 2 years after the day of manufacturing. |
Required but not provided: | 50 碌L micropipettor and pipette tips 8-channel (50-200 碌L) micropipettor and tips 8 or 12-syncronous multichannel pipet and reagent tray for multichannel pipet. 4-7 掳C incubator Microtiter plate reader (measuring wavelength 450 nm) |
技术参数
检测原理:竞争法ELISA (检测: 450 nm处);
特异性:特异于N-epsilon-己酰-赖氨酸加合物;
测定范围:2 – 700 nmol/L;
反应速度:过夜+2小时;
规格:96孔(54 samples in single assay);
应用:人和动物的尿液、血清和细胞培养物;
储存:2-8℃(避免冷冻);
保质期:2年。
Content of this kit
HEL-coated Microtiter Plate: | 1 plate (96 wells) | |
Primary Antibody (ready to use): | 1 vial | |
Secondary Antibody: | 1 vial | |
Secondary Antibody Buffer: | 1 vial | |
Chromogen (TMBZ solution): | 1 vial | |
Chromogen Buffer: | 1 vial | |
Washing Buffer (5X): | 1 vial | |
Stop Solution: | 1 vial | |
Standard solution (6 levels): | 1 vial each | |
Plate seal: | 2 sheets |
试剂盒组成:
HEL-包被微孔板: 1 块板(96孔)
一抗 (即用型): 1 瓶
二抗: 1 瓶
二抗缓冲液: 1 瓶
底物溶液 (TMBZ溶液): 1 瓶
底物缓冲液: 1 瓶
洗涤液 (5X): 1 瓶
终止液: 1 瓶
标准液 (6 标准): 各1 瓶
封板膜: 2 片
Product name | Code | Assay range | Application |
Hexanoyl-Lys (HEL) ELISA | KHL-700E | 2-700 nmol/L | Urine, serum, cultured cells and other biological materials |
参考文献:
1)
Yoji Kato, Yoko Mori, Yuko Makino, Yasujiro Morimitsu, Sadayuki Hiroi, Toshitsugu Ishikawa, Toshihiko Osawa, Formation of N epsilon-(hexanonyl) lysine in protein exposed to lipid hydroperoxide. J Biol Chem 274(29), p20406-20414 (1999)
Identification of HEL, which is lysine adduct of 13-HPODE.
2)
Yoji Kato, Toshihiko Osawa: Detection of lipid hydroperoxide-derived protein modification with polyclonal antibodies. Methods in Molecular Biology, vol. 186, Oxidative stress biomarkers and antioxidant protocols, D Armstrong, Ed., Human Press Inc., NJ, USA, p37-44
Immunohistochemical detection of hexanoyl-lysine adduct.
3)
Kato Y, Miyake Y, Yamamoto K, Shimomura Y, Ochi H, Mori Y, Osawa T, Preparation of a monoclonal antibody to N(epsilon)-(Hexanonyl)lysine: application to the evaluation of protective effects of flavonoid supplementation against exercise-induced oxidative stress in rat skeletal muscle. Biochem Biophys Res Commun 274(2),p389-393(2000)
Development and characterization of anti HEL monoclonal antibody.
4)
Ryo K, Yamada H, Nakagawa Y, Tai Y, Obara K, Inoue H, Mishima K, Saito I, Possible involvement of oxidative stress in salivary gland of patients with Sjogren’s syndrome. Pathobiology 73(5), p252-260 (2006)
HEL can be detected in saliva samples from patients with Sjogren’s syndrome.
5)
Suzuki T, Kazui T, Yamamoto S, Washiyama N, Ohkura K, Ohishi K, Bashar AH, Yamashita K, Terada H, Suzuki K, Akuzawa S, Fujie M, Effect of prophylactically administered edaravone during antegrade cerebral perfusion in a canine model of old cerebral infarction. J Thorac Cardiovasc Surg 133(3),p710-716 (2007)
Application to canine serum samples.
6)
Shimizu K, Ogawa F, Akiyama Y, Muroi E, Yoshizaki A, Iwata Y, Komura K, Bae S, Sato S, Increased Serum Levels of N(epsilon)-(hexanoyl)lysine, A New Marker of Oxidative Stress, in Systemic Sclerosis. J Rheumatol. 2008 Sep 1.
Serum HEL concentration from systemic sclerosis is higher than that from healthy subjects.
7)
Tamura H, Takasaki A, Miwa I, Taniguchi K, Maekawa R, Asada H, Taketani T, Matsuoka A, Yamagata Y, Shimamura K, Morioka H, Ishikawa H, Reiter RJ, Sugino N, Oxidative stress impairs oocyte quality and melatonin protects oocytes from free radical damage and improves fertilization rate. J Pineal Res 44(3),p280-287(2008)
Intrafollicular concentration of HEL was significantly reduced by these antioxidant treatment.
8)
Sakamoto Y, Ishikawa T, Kondo Y, Yamaguchi K, Fujisawa M, The assessment of oxidative stress in infertile patients with varicocele. BJU Int 101(12), p1547-1552 (2008)
Azoospermic and oligospermic patients had a significantly higher HEL concentration in seminal plasma.
9)
Kageyama Y, Takahashi M, Nagafusa T, Torikai E, Nagano A, Etanercept reduces the oxidative stress marker levels in patients with rheumatoid arthritis. Rheumatol Int 28(3),pp245-251(2008)
Urinary HEL level was reduced at 3 and 6 months after the initial treatment with etanercept.
10)
Rummenie VT, Matsumoto Y, Dogru M, Wang Y, Hu Y, Ward SK, Igarashi A, Wakamatsu T, Ibrahim O, Goto E, Luyten G, Inoue H, Saito I, Shimazaki J, Tsubota K, Tear cytokine and ocular surface alterations following brief passive cigarette smoke exposure. Cytokine 43(2),p200-208(2008)
HEL concentration in tear was increased by brief passive exposure to cigarette smoke.
11)
K Sakai, S Kino, A Masuda, M Takeuchi, T Ochi, J Osredkar, B Rejc, K Gersak, N Ramarathnam, Y Kato;
Determination of HEL (Hexanoyl-Lysine Adduct): A Novel Biomarker for Omega-6 PUFA Oxidation.
Lipid Hydroperoxide-Derived Modification of Biomolecules: Subcellular Biochemistry 77,p61-72(2014)
An original paper for HEL ELISA development.
- 尿8-异前列腺素(15-异前列腺素F2t)ELISA试剂盒 NWLSS™ Urinary 8-Isoprostane
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- 己酰赖氨酸ELISA试剂盒 NWLSS™ Hexanoyl Lysine ELISA Kit
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Instruction manual | Quality control | Technical information |
Whatman10443004玻璃真空过滤装置GV100/0/05 CENTRING F/FRT 1/PK
【简单介绍】
【简单介绍】
【详细说明】
上海金畔生物科技有限公司
文章号19845089-19845089
thermo改良的Lowry蛋白定量分析试剂 货号:2324023240-赛默飞中国代理商
产品名称:
|
thermo改良的Lowry蛋白定量分析试剂 货号:23240 |
产品型号:
|
23240 |
产品特点 |
Z广泛引用的比色方法;在750纳米处读数。对于经典的Lowry方法使用者来说为即用型试剂使用更加方便。预先配制好的硫酸化酮酒石酸盐试剂室温下稳定一年。BSA的线性范围为10-1,500 微克/毫升。方便的微孔板和试管形式。比染料结合方法的蛋白间差异小。 |
产品详细信息: |
|
Omnipore表面滤膜JHWP04700,PTFE滤膜0.45um孔径JHWP04700
Omnipore表面滤膜JHWP04700,PTFE滤膜0.45um孔径JHWP04700
说明:Omnipore Membrane, PTFE, hydrophilic, 0.45 µm, 47 mm, white, plain
商标名:Omnipore
数量/包装:100
滤膜材质:Hydrophilic PTFE
zui高操作温度,°C:130
滤膜类型:表面滤膜
滤膜孔径,µm:0.45
可润湿性:亲水
滤膜直径,mm:47
滤膜代码:JHWP
滤膜颜色:白色
产品名称:Omnipore Membrane Filter
滤膜表面:光面
孔隙率 %:80
Whatman1882-047硼硅酸纤维滤纸 EPM-2000 4.7CM 100/PK
【简单介绍】
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【详细说明】
上海金畔生物科技有限公司
文章号:20623048-20623048
Whatman10400914混合纤维素酯膜 ME27 0.8um 50MM 100/PK
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【简单介绍】
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上海金畔生物科技有限公司
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【简单介绍】
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上海金畔生物科技有限公司
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Whatman1950-209三件套过滤漏斗 3 PIECE 9CM RESERVOIR 1/PK
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上海金畔生物科技有限公司
文章号19831152-19831152
WhatmanWB100078FTA微型卡HARRIS 25XUNI-CORE 3.00MM
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上海金畔生物科技有限公司
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Whatman1444-150Grade 44 定量滤纸 GR 44 15CM 100/PK
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上海金畔生物科技有限公司
文章号20337814-20337814
NS神经细胞基础培养基
NS神经细胞基础培养基
NS Supplement
- 产品特性
- 相关资料
- Q&A
- 参考文献
NS基础培养基/NS Supplement
用于神经细胞/神经干细胞培养
NS基础培养基是、最适合用于神经细胞培养的基础培养基。NS Supplement是用于神经细胞培养的无血清Supplement(含有维生素A乙酸酯Retinyl Acetate)。适用于从大鼠海马分离出的神经细胞培养、神经干细胞等的培养。请将NS基础培养基和NS Supplement混合后使用。
◆来自大鼠海马的初代神经细胞培养
●在Poly-L-lysine涂层板上培养了从大鼠胎儿(E19)海马分离出来的神经细胞。
●观察到培养6天后的细胞形态、培养21天后的神经细胞标记(Map2(a+b))以及胶质细胞标记(GFAP)。
培养6天后
NS基础培养基/NSSupplement 同等同行培养基/Supplement
( 培养基组成 )
NS基础培养基 + 2% NS Supplement + 0.5mmol/l L-谷氨酰胺
( 细胞播种数 )
13,000cells/well(96孔细胞培养板)
培养21天后
◆来自大鼠大脑皮质的初代神经细胞培养
●在Poly-L-lysine涂层板上培养了从大鼠胎儿(E17)大脑皮质分离出来的神经细胞。
●观察到培养8天后的细胞形态、培养14天后的神经细胞标记(Map2(a+b))以及胶质细胞标记(GFAP)。
培养8天后
( 培养基组成 )
NS基础培养基 + 2% NS Supplement + 0.5mmol/l L-谷氨酰胺
( 细胞播种数)
60,000cells/well(96孔细胞培养板)
培养14天后
产品编号 | 产品名称 | 产品规格 | 产品等级 | 产品价格 |
148-09615 | NS Basal Medium | 500ml | 用于细胞培养 | – |
146-09351 | NS Supplement(×50) | 10ml | 用于细胞培养 | – |
073-05391 | 200mmol/l L-Glutamin Solution(×100) | 100ml | 用于细胞培养 | – |
141-08941 | N2 Supplement with Transferrin(Holo) | 5ml | 用于细胞培养 | – |
141-09041 | N2 Supplement with Transferrin(Apo) | 5ml | 用于细胞培养 | – |