日本同仁化学Hampton蛋白结晶试剂盒4 Inch Soft Rubber Brayer- DOJINDO

上海金畔生物科技有限公司代理日本同仁化学 DOJINDO代理商全线产品,欢迎访问官网了解更多信息

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等,我们将竭诚为您服务,欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。Products > Crystallization Plates, Hardware & Accessories > Sealing Films, Tapes, Mats & Covers > 4 Inch Soft Rubber Brayer

4 Inch Soft Rubber Brayer

Applications

  • Even and secure application of sealing films to crystallization plates

Features

  • 4 inch wide soft roller with metal handle

Description

Soft rubber roller ideal for use with self adhesive sealing films and foils. The brayer allows for even and secure application of sealing films to 24, 48, 96, 384 and 1536 micrplate format crystallization plates. The four inch wide soft roller applies even pressure to help ensure a uniform and complete seal without the risk of the film touching sitting drops. The brayer is also useful for sealing the LCP Sandwich Set glass plates (HR3-151).

Hampton蛋白结晶试剂盒4 Inch Soft Rubber Brayer

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Hampton蛋白结晶试剂盒4 Inch Soft Rubber Brayer
Hampton蛋白结晶试剂盒4 Inch Soft Rubber Brayer

CAT NO

HR4-470

NAME

4 Inch Soft Rubber Brayer

DESCRIPTION

each

PRICE

$32.00

cart quote

UTP酶试剂盒Takara


UTP
品牌 Code No. 产品名称 包装量 价格(元) 说明书 数量
Takara 4044 UTP 25 μmol ¥426 UTP UTP UTP
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□ 浓度 100 mM
□ 贮存溶液 水溶液 (Na盐),pH7.0±0.1
 
■ 保存
-20℃。
 
■ 纯度
≥95%
 
■ 用途
本制品溶于含Na盐的水溶液中,使用时请选择合适的Buffer稀释。
 
 

页面更新:2017-08-10 15:57:35

日本同仁化学Hampton蛋白结晶试剂盒Film Sealing Paddle- DOJINDO

上海金畔生物科技有限公司代理日本同仁化学 DOJINDO代理商全线产品,欢迎访问官网了解更多信息

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等,我们将竭诚为您服务,欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。Products > Crystallization Plates, Hardware & Accessories > Sealing Films, Tapes, Mats & Covers > Film Sealing Paddle

Film Sealing Paddle

Applications

  • Even and secure application of sealing films to crystallization plates

Features

  • White, polyethyelene plastic
    • 5 paddles per pack
    • 2.958″ short side width, 3.250″ long side width, 0.125″ thick
    • Less firm than gold paddle
  • Gold, plastic, nylon reinforced edge
    • 1 paddle per pack
    • 3 7/8″ wide x 2 15/16″ tall
    • More firm than white paddle

Description

An alternative to the plate roller or brayer for pressing films to assure a secure uniform seal around all wells on 96-well, 384-well, and 1536-well plates. These paddles are especially recommended for sealing films on raised-rim plates because they fit within the rim of the plate.

White, film sealing paddle, polyethylene, less firm than gold paddle. 5 paddles per pack. 

Gold, film sealing paddle, plastic, nylon reinforced edge, more firm than white paddle. 1 paddle per pack. 

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Hampton蛋白结晶试剂盒Film Sealing Paddle

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Hampton蛋白结晶试剂盒Film Sealing Paddle
Hampton蛋白结晶试剂盒Film Sealing Paddle
Hampton蛋白结晶试剂盒Film Sealing Paddle
Hampton蛋白结晶试剂盒Film Sealing Paddle
Hampton蛋白结晶试剂盒Film Sealing Paddle
Hampton蛋白结晶试剂盒Film Sealing Paddle

CAT NO

HR4-413

NAME

Film Sealing Paddle – White

DESCRIPTION

5 pack

PRICE

$9.00

cart quote

CAT NO

HR4-525

NAME

3M Sealing Paddle – Gold

DESCRIPTION

Each

PRICE

$8.00

cart quote

Related Item(S)

  • Crystal Clear Sealing Tape
  • Crystal Clear Sealing Film
  • AlumaSeal™ II Sealing Film and Applicator
  • Film Sealing Paddle

References

 

IPTG酶试剂盒Takara


IPTG
品牌 Code No. 产品名称 包装量 价格(元) 说明书 数量
Takara 9030 IPTG (Dioxane free) 5 g ¥481 IPTG IPTG IPTG
收藏产品 加入购物车
 
□ 形态 白色粉末
□ 规格 分子量238.31
 
制品说明
IPTG是β-半乳糖苷酶的活性诱导物,基于这个特性,当pUC系列的载体以lacZ缺欠细胞作为宿主进行转化时、或用M13噬菌体载体DNA进行转染时,如果在培养基中加入X-Gal 和IPTG,由于β-半乳糖苷酶的α-互补性,可以根据是否呈现白色而方便地挑选择出基因重组体。另外,它还可以作为具有lactac等启动子的表达载体的表达诱导物使用。
 
保存
-20℃。
 
用途
IPTG是细菌中β-半乳糖苷酶的活性诱导物质。基于这个特性,当使用pUC系列的载体转化或使用M13噬菌体的载体转导lacZ宿主细胞时,通过在培养基中添加X-Gal (Code No.: 9031) 和IPTG,由于β-半乳糖苷酶的α-互补性,可以根据是否呈现白色菌落 (或噬菌斑) 而方便地挑选出基因重组体。此外,它还可以作为具有lactac等启动子的表达载体的表达诱导物使用。
 
使用方法
溶于灭菌水配制成100 mM水溶液 (每10 ml灭菌水加0.238 g IPTG)。
过滤灭菌后,将溶液按1ml分装,-20℃保存。 (含有IPTG的培养基4℃保存,避光,配制后一周内使用。)
 
工作浓度
1) 质粒
·Agar medium; 100 μl/ 100 ml agar medium
·Plate (直径9 cm ); 25 μl/ plate
2) 噬菌体
·Top agar; 25 μl/ 3 ml top agar
 
 

页面更新:2019-08-09 09:58:24

日本同仁化学Hampton蛋白结晶试剂盒Dow Corning® Vacuum Grease- DOJINDO

上海金畔生物科技有限公司代理日本同仁化学 DOJINDO代理商全线产品,欢迎访问官网了解更多信息

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等,我们将竭诚为您服务,欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。Products > Crystallization Plates, Hardware & Accessories > Sealants, Sealing Grease & Oils > Dow Corning® Vacuum Grease

Dow Corning® Vacuum Grease

Applications

  • Standard sealant for hanging, sitting, and sandwich drop vapor diffusion set ups.

Description

Dow Corning® Vacuum Grease. The standard sealant for hanging, sitting, and sandwich drop vapor diffusion setups. Stiff, non-melting, non-drying silicone sealant maintains its consistency from -40° to 260°C. Chemical-resistant and non-carbonaceous. Squeeze the grease into a 10 cc syringe fitted with a 200 µl pipet tip trimmed to desired diameter and you are ready to grease.

Hampton蛋白结晶试剂盒Dow Corning® Vacuum Grease

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Hampton蛋白结晶试剂盒Dow Corning® Vacuum Grease

CAT NO

HR3-510

NAME

Dow Corning Vacuum Grease

DESCRIPTION

150 g tube

PRICE

$44.00

cart quote

Support Material(s)

Hampton蛋白结晶试剂盒Dow Corning® Vacuum Grease HR3-510 Dow Corning Vacuum Grease SDS

X-Gal酶试剂盒Takara


X-Gal
品牌 Code No. 产品名称 包装量 价格(元) 说明书 数量
Takara 9031 X-Gal 1 g ¥481 X-Gal X-Gal X-Gal
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□ 形态 白色粉末
□ 规格 分子量 408.6
 
制品说明
X-Gal是β-半乳糖苷酶的底物,水解后呈蓝色。基于这个特点,当pUC系列载体DNA以lacZ缺欠细胞作为宿主进行转化时,或M13噬菌体载体DNA进行转染时,如果在培养基中加入X-Gal和IPTG,由于β-半乳糖苷酶的α-互补性,可以根据是否呈现白色而方便地挑选择出基因重组体。
 
用途
X-Gal是β-半乳糖苷酶 (β-galactosidase) 的底物,水解后呈蓝色。基于这个特点,pUC系列载体DNA以lacZ 缺失细胞为宿主进行转化时、或用M13噬菌体载体DNA进行转染时,如果在平板培养基中加入X-Gal和IPTG (Code No.: 9030),由于β-半乳糖苷酶的α-互补性,可以根据是否呈现白色菌落 (或噬菌斑) 而方便地挑选出基因重组体。
 
使用方法
首先把X-Gal配制成20 mg/ml的二甲基甲酰胺 (DMF) 溶液,将溶液-20℃避光保存。
(含有X-Gal的培养基4℃避光保存,须在1周内使用。)
 
工作浓度
1) 质粒
· Agar medium; 200 μl/ 100 ml agar medium
· Plate (直径9 cm ); 50 μl/ plate
2) 噬菌体
· Top agar; 50 μl/ 3 ml top agar
 
 

页面更新:2019-08-09 13:53:27

日本同仁化学Hampton蛋白结晶试剂盒Dow Corning® 7 Release Compound Grease- DOJINDO

上海金畔生物科技有限公司代理日本同仁化学 DOJINDO代理商全线产品,欢迎访问官网了解更多信息

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等,我们将竭诚为您服务,欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。Products > Crystallization Plates, Hardware & Accessories > Sealants, Sealing Grease & Oils > Dow Corning® 7 Release Compound Grease

Dow Corning® 7 Release Compound Grease

Applications

  • Grease sealant for hanging, sitting, and sandwich drop vapor diffusion set ups.

Description

The Dow Corning® 7 Release Compound Grease sealant is similar to vacuum grease but is less viscous. Being less viscous, it is easier to push through a syringe when applying the sealant manually. Squeeze the grease into a 10 cc syringe fitted with a 200 µl pipet tip trimmed to desired diameter and you are ready to grease.

Hampton蛋白结晶试剂盒Dow Corning® 7 Release Compound Grease

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Hampton蛋白结晶试剂盒Dow Corning® 7 Release Compound Grease

CAT NO

HR3-508

NAME

Dow Corning 7 Release Compound Grease

DESCRIPTION

150 g tube

PRICE

$35.00

cart quote

Support Material(s)

Hampton蛋白结晶试剂盒Dow Corning® 7 Release Compound Grease HR3-508 Dow Corning 7 Release Compound Grease SDS

Bovine Serum Albumin (BSA)酶试剂盒Takara


Bovine Serum Albumin (BSA)
品牌 Code No. 产品名称 包装量 价格(元) 说明书 数量
Takara 2320 Bovine Serum Albumin (BSA) 1 ml ¥601 Bovine Serum Albumin (BSA) Bovine Serum Albumin (BSA) Bovine Serum Albumin (BSA)
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□ 浓度 20 mg/ml
□ 贮存溶液  
     Tris-HCl (pH 7.5) 10 mM
     EDTA 1 mM
     甘油 50 %
 
■ 制品说明
本制品是由小牛血清经过高度精制而成的,用于基因工程研究。
 
■ 保存
-20℃。
 
■ 来源
Bovine serum
 
■ 应用
本制品作为稳定剂被用于Takara Bio限制酶和修饰酶的保存溶液和反应液中。
 
 

页面更新:2019-03-13 15:06:44

日本同仁化学Hampton蛋白结晶试剂盒Replacement Grease Cartridges- DOJINDO

上海金畔生物科技有限公司代理日本同仁化学 DOJINDO代理商全线产品,欢迎访问官网了解更多信息

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等,我们将竭诚为您服务,欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。Products > Crystallization Plates, Hardware & Accessories > Sealants, Sealing Grease & Oils > Replacement Grease Cartridges

Replacement Grease Cartridges

Applications

  • For the Grease Machine L-100

Description

Replacement grease cartridges for the Grease Machine L-100.

Hampton蛋白结晶试剂盒Replacement Grease Cartridges

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Hampton蛋白结晶试剂盒Replacement Grease Cartridges

CAT NO

HR3-202

NAME

Grease Cartridge

DESCRIPTION

5 pack

PRICE

$73.00

cart quote

Genetic research kit酶试剂盒Takara


Genetic research kit
品牌 Code No. 产品名称 包装量 价格(元) 说明书 数量
Takara 6999Q-3386 Genetic research kit 1 ml 询价 Genetic research kit Genetic research kit Genetic research kit
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无标题文档

 
□ 浓度 50 mg/ml
□ 贮存溶液  
     Tris-HCl (pH 7.5) 10 mM
     EDTA 1 mM
     甘油 50 %
 
■ 制品说明
本制品是由小牛血清经过高度精制而成的,用于基因工程研究。
 
■ 保存
-20℃。
 
■ 来源
Bovine serum
 
■ 纯度
1. 200 μg 的本制品和1 μg 的λ DNA-Hind III 分解物在37℃、pH7.5 的条件下反应16小时, DNA片段的电泳图像不起变化。
2. 200 μg 的本制品和1 μg 的pBR322 超螺旋DNA在37℃、pH7.5 的条件下反应4小时,不发生DNA的解螺旋现象。
3. 200 μg 的本制品和1 μg 的16S,23S rRNA在37℃、pH7.5的条件下反应1小时,RNA片段的电泳图像不起变化。
 
■ 应用
本制品作为稳定剂被用于Takara Bio限制酶和修饰酶的保存溶液和反应液中。
 
 

页面更新:2019-11-05 15:17:15

日本同仁化学Hampton蛋白结晶试剂盒12 mm Siliconized Glass Cover Slides- DOJINDO

上海金畔生物科技有限公司代理日本同仁化学 DOJINDO代理商全线产品,欢迎访问官网了解更多信息

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等,我们将竭诚为您服务,欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。Products > Crystallization Plates, Hardware & Accessories > Cover Slides & Related Tools > 12 mm Siliconized Glass Cover Slides

12 mm Siliconized Glass Cover Slides

Applications

  • Siliconized glass cover slides for hanging & sitting drop crystallization
  • Hanging drop crystallization
  • Sitting drop crystallization
  • Sandwich drop crystallization

Features

  • High purity of glass
  • Siliconized, hydrophobic glass surface
  • 0.22 mm & 0.96 mm glass thickness
  • 12 mm glass diameter
  • Circle
  • 0.22 mm glass thickness is UV Compatible (>85% transmission)

Description

Siliconized glass cover slides allow a droplet to be suspended in a position which provides near optimal conditions for vapor diffusion with the surrounding reservoir solution.

These siliconized glass cover slides provide a consistent, high quality finish for crystallization experiments. The hydrophobic surface produces a drop which “stands well” and does not flatten on the glass. The siliconized surface prevents the adhesion of crystals and precipitate onto the glass surface. Use Dow Corning Vacuum Grease (HR3-510) or Dow Corning 7 Release Compound (HR3-508) to seal cover slide to plate. Available in 12, 18 or 22 mm diameter circles and 22 mm diameter squares. Available in 0.22 mm or 0.96 mm glass thickness. The 0.96 mm thick slides are virtually unbreakable during crystallization handling.

Critical surface tension Untreated glass 78 dynes/cm Siliconized glass 31 dynes/cm Surface resistivity Untreated glass 1×1012 ohms Siliconized glass 1.2 x 1013 ohms Coefficient of friction static, glass slide on glass slide Untreated 0.9-1.0 Siliconized 0.2-0.3 Protein adsorption (100 hours) Untreated glass 0.13 mg/mm2 Siliconized glass 0.01-0.02 mg/mm2

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Hampton蛋白结晶试剂盒12 mm Siliconized Glass Cover Slides

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Hampton蛋白结晶试剂盒12 mm Siliconized Glass Cover Slides
Hampton蛋白结晶试剂盒12 mm Siliconized Glass Cover Slides

CAT NO

HR3-278T

NAME

Siliconized circle cover slides for Tecan robot

DESCRIPTION

0.5 oz pack (~240 slides)

PRICE

$54.00

cart quote

CAT NO

HR3-277

NAME

12 mm x 0.22 mm Siliconized circle cover slides

DESCRIPTION

0.5 ounce pack (~240 slides)

PRICE

$54.00

cart quote

CAT NO

HR3-279

NAME

12 mm x 0.22 mm Siliconized circle cover slides

DESCRIPTION

5.0 ounce case (~2,400 slides)

PRICE

$519.00

cart quote

CAT NO

HR8-088

NAME

12mmx0.96mmThick Siliconized circle cover slides

DESCRIPTION

1.0 ounce pack (~106 slides)

PRICE

$32.00

cart quote

CAT NO

HR8-090

NAME

12mmx0.96mm Thick Siliconized circle cover slides

DESCRIPTION

10.0 ounce case (~1,060 slides)

PRICE

$313.00

cart quote

Related Item(S)

  • VDX48 Plate with sealant

References

 

Proteinase K酶试剂盒Takara


Proteinase K
品牌 Code No. 产品名称 包装量 价格(元) 说明书 数量
Takara 9034 Proteinase K 5 ml ¥450 Proteinase K Proteinase K Proteinase K
收藏产品 加入购物车

无标题文档

 
■ 制品说明
本制品是以丝氨酸为活性中心的蛋白分解酶,分解蛋白质的活性较强。本制品在含Ca2+盐的溶液中活性稳定,并且在SDS或尿素等蛋白质变性剂存在下活性升高。具有广泛的底物特异性,特别优先分解与疏水性氨基酸、含硫氨基酸、芳香族氨基酸的C末端邻接的酯键和肽键。
 
■ 活性定义
在37℃ pH7.5的条件下,1分钟内生成相当于1.0 μmol酪氨酸的folin positive amino acids所需的酶量定义为1个活性单位(U)。
 
■ 用途
1. DNA、RNA和Phage的提取。
2. In situ hybridization、Finger printing、Colony hybridization、plaque hybridization、调制脉冲电场凝胶电泳用的染色体DNA。
 
■ 保存
4℃
 

页面更新:2022-05-11 16:21:03

日本同仁化学Hampton蛋白结晶试剂盒18 mm Siliconized Glass Cover Slides- DOJINDO

上海金畔生物科技有限公司代理日本同仁化学 DOJINDO代理商全线产品,欢迎访问官网了解更多信息

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等,我们将竭诚为您服务,欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。Products > Crystallization Plates, Hardware & Accessories > Cover Slides & Related Tools > 18 mm Siliconized Glass Cover Slides

18 mm Siliconized Glass Cover Slides

Applications

  • Siliconized glass cover slides for hanging & sitting drop crystallization
  • Hanging drop crystallization
  • Sitting drop crystallization
  • Sandwich drop crystallization

Features

  • High purity of glass
  • Siliconized, hydrophobic glass surface
  • 0.22 mm & 0.96 mm glass thickness
  • 18 mm glass diameter
  • Circle
  • 0.22 mm glass thickness is UV Compatible (>85% transmission)

Description

Siliconized glass cover slides allow a droplet to be suspended in a position which provides near optimal conditions for vapor diffusion with the surrounding reservoir solution.

These siliconized glass cover slides provide a consistent, high quality finish for crystallization experiments. The hydrophobic surface produces a drop which “stands well” and does not flatten on the glass. The siliconized surface prevents the adhesion of crystals and precipitate onto the glass surface. Use Dow Corning Vacuum Grease (HR3-510) or Dow Corning 7 Release Compound (HR3-508) to seal cover slide to plate. Available in 12, 18 or 22 mm diameter circles and 22 mm diameter squares. Available in 0.22 mm or 0.96 mm glass thickness. The 0.96 mm thick slides are virtually unbreakable during crystallization handling.

Critical surface tension Untreated glass 78 dynes/cm Siliconized glass 31 dynes/cm Surface resistivity Untreated glass 1×1012 ohms Siliconized glass 1.2 x 1013 ohms Coefficient of friction static, glass slide on glass slide Untreated 0.9-1.0 Siliconized 0.2-0.3 Protein adsorption (100 hours) Untreated glass 0.13 mg/mm2 Siliconized glass 0.01-0.02 mg/mm2

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Hampton蛋白结晶试剂盒18 mm Siliconized Glass Cover Slides

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Hampton蛋白结晶试剂盒18 mm Siliconized Glass Cover Slides
Hampton蛋白结晶试剂盒18 mm Siliconized Glass Cover Slides

CAT NO

HR3-239

NAME

18 mm x 0.22 mm Siliconized circle cover slides

DESCRIPTION

0.5 ounce pack (~100 slides)

PRICE

$29.00

cart quote

CAT NO

HR3-241

NAME

18 mm x 0.22 mm Siliconized circle cover slides

DESCRIPTION

5.0 ounce case (~1,000 slides)

PRICE

$276.00

cart quote

CAT NO

HR3-515

NAME

18mmx0.96mm Thick Siliconized circle cover slides

DESCRIPTION

1 oz pack (~45 slides)

PRICE

$27.00

cart quote

CAT NO

HR3-517

NAME

18mmx0.96mm Thick Siliconized circle cover slides

DESCRIPTION

10 oz case (~450 slides)

PRICE

$249.00

cart quote

References

 

Yatalase-Plus-酶试剂盒Takara


Yatalase-Plus-
品牌 Code No. 产品名称 包装量 价格(元) 说明书 数量
OZK T030 Yatalase-Plus- 2 g ¥5,612 Yatalase-Plus- Yatalase-Plus- Yatalase-Plus-
OZK T030B (T030 × 2) Yatalase-Plus- 4 g ¥10,102 Yatalase-Plus- Yatalase-Plus- Yatalase-Plus-
收藏产品 加入购物车
 
■ 制品说明
本制品是以Chitinase,Chitobiase和α-1,3-Glucanase为主要成分的复合酶制剂。
◇ 具有Chitinase,Chitobiase和α-1,3-Glucanase的活性。
◇ 单独使用即可制备丝状真菌的原生质体。对于使用Yatalase(Code No. T017)难以制备原生质体的黑曲霉等丝状真菌,也能有效使其原生质体化。
 
■ 保存
4℃,干燥条件。
 
■ 形态
干粉(含乳糖作为辅料)
 
■ 酶活性
Chitinase活性: 50 U/g powder 以上
Chitobiase活性:500 U/g powder 以上
α-1,3-Glucanase活性: 20 U/g powder 以上
 
■ 活性定义
[Chitinase活性]
1 U定义:37℃条件下,1分钟内从pH6.0的0.5%甲壳素(chitin powder)溶液中释放1 μmol的游离N–Acetylglucosamine所需要的酶量。
 
[Chitobiase活性]
1 U的定义:37℃条件下,1分钟内从pH6.0的5 mM p-Nitrophenyl-N-acetyl-β-D-glucosaminide溶液中释放1 μmol的游离p-Nitrophenol所需要的酶量。
 
[α-1,3-Glucanase活性]
1 U的定义:30℃条件下,1分钟内从pH6.0的2% α-1,3-glucan悬浊液中释放1 μmol游离葡萄糖所需要的酶量。
 
■ 一般性质
最适pH : pH6.0
最适温度 : 30℃
 
Yatalase-Plus-
 
■ 针对各种曲霉属的原生质体形成能力
菌株 原生质体形成
Yatalase Yatalase-Plus-
Aspergillus oryzae
Aspergillus niger ×
Aspergillus luchuensis ×
Aspergillus sojae
Aspergillus nidulans
Aspergillus kawachii ×
Aspergillus aculeatus ×
○:很好 △:一般 ×:较差
Yatalase -Plus-对于使用Yatalase(Code No. T017)难以制备原生质体的黑曲霉等丝状真菌效果更好。
 
本制品由Ozeki corporation制造。
 
 

页面更新:2020-10-20 16:34:25

日本同仁化学Hampton蛋白结晶试剂盒22 mm Siliconized Glass Cover Slides- DOJINDO

上海金畔生物科技有限公司代理日本同仁化学 DOJINDO代理商全线产品,欢迎访问官网了解更多信息

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等,我们将竭诚为您服务,欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。Products > Crystallization Plates, Hardware & Accessories > Cover Slides & Related Tools > 22 mm Siliconized Glass Cover Slides

22 mm Siliconized Glass Cover Slides

Applications

  • Siliconized glass cover slides for hanging & sitting drop crystallization.
  • Hanging drop crystallization
  • Sitting drop crystallization
  • Sandwich drop crystallization

Features

  • High purity of glass
  • Siliconized, hydrophobic glass surface.
  • 0.22 mm & 0.96 mm glass thickness
  • 22 mm glass diameter
  • Circle & square
  • 0.22 mm glass thickness is UV Compatible (>85% transmission)

Description

Siliconized glass cover slides allow a droplet to be suspended in a position which provides near optimal conditions for vapor diffusion with the surrounding reservoir solution.

These siliconized glass cover slides provide a consistent, high quality finish for crystallization experiments. The hydrophobic surface produces a drop which “stands well” and does not flatten on the glass. The siliconized surface prevents the adhesion of crystals and precipitate onto the glass surface. Use Dow Corning Vacuum Grease (HR3-510) or Dow Corning 7 Release Compound (HR3-508) to seal cover slide to plate. Available in 12, 18 or 22 mm diameter circles and 22 mm diameter squares. Available in 0.22 mm or 0.96 mm glass thickness. The 0.96 mm thick slides are virtually unbreakable during crystallization handling.

Critical surface tension Untreated glass 78 dynes/cm Siliconized glass 31 dynes/cm Surface resistivity Untreated glass 1×1012 ohms Siliconized glass 1.2 x 1013 ohms Coefficient of friction static, glass slide on glass slide Untreated 0.9-1.0 Siliconized 0.2-0.3 Protein adsorption (100 hours) Untreated glass 0.13 mg/mm2 Siliconized glass 0.01-0.02 mg/mm2

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Hampton蛋白结晶试剂盒22 mm Siliconized Glass Cover Slides

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Hampton蛋白结晶试剂盒22 mm Siliconized Glass Cover Slides
Hampton蛋白结晶试剂盒22 mm Siliconized Glass Cover Slides

CAT NO

HR3-231

NAME

22 mm x 0.22 mm Siliconized circle cover slides

DESCRIPTION

1.0 ounce pack (~120 slides)

PRICE

$32.00

cart quote

CAT NO

HR3-233

NAME

22 mm x 0.22 mm Siliconized circle cover slides

DESCRIPTION

10.0 ounce case (~1,200 slides)

PRICE

$313.00

cart quote

CAT NO

HR3-247

NAME

22 mm x 0.96 mm Thick Siliconized circle cover slides

DESCRIPTION

3 ounce pack (~75 slides)

PRICE

$35.00

cart quote

CAT NO

HR3-249

NAME

22mmx 0.96mm Thick Siliconized circle cover slides

DESCRIPTION

30.0 ounce case (~750 slides)

PRICE

$339.00

cart quote

CAT NO

HR3-215

NAME

22 mm x 0.22 mm Siliconized square cover slides

DESCRIPTION

1.0 ounce pack (~100 slides)

PRICE

$29.00

cart quote

CAT NO

HR3-217

NAME

22 mm x 0.22 mm Siliconized square cover slides

DESCRIPTION

10.0 ounce case (~1,000 slides)

PRICE

$276.00

cart quote

CAT NO

HR3-223

NAME

22mmx0.96mm Thick Siliconized square cover slides

DESCRIPTION

4.0 ounce pack (~75 slides)

PRICE

$32.00

cart quote

CAT NO

HR3-225

NAME

22mmx0.96mm Thick Siliconized square cover slides

DESCRIPTION

40.0 ounce case (~750 slides)

PRICE

$313.00

cart quote

References

 

日本同仁化学Hampton蛋白结晶试剂盒Glass Cover Slide Gizmo Dispenser- DOJINDO

上海金畔生物科技有限公司代理日本同仁化学 DOJINDO代理商全线产品,欢迎访问官网了解更多信息

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等,我们将竭诚为您服务,欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。Products > Crystallization Plates, Hardware & Accessories > Cover Slides & Related Tools > Glass Cover Slide Gizmo Dispenser

Glass Cover Slide Gizmo Dispenser

Applications

  • Dispense circle or square cover slides for hanging drop crystallization

Features

  • Dispense siliconized or plain cover slides
  • Platform displays one to six cover slides at a time for 18 and 22 mm slides
  • Black platform for slides allows one to see if drops are clear or precipitated
  • Available for 12, 18 or 22 mm diameter cover slides for either original (0.22 mm) and thick (0.96 mm)
  • Platform displays one to twelve cover slides at a time for 12 mm slides

Description

The Cover Slide Gizmo dispenses siliconized or plain slides for convenient drop building during hanging drop vapor diffusion crystallization. Load the cover slides into the dispenser. A clear plastic cover or sealed dispenser keeps slides clean. Slide the loaded dispenser along the platform and six cover slides are dispensed onto the platform. A black background on the platform makes for easy drop visualization as one builds the drop(s). Use finger or forceps to place the completed cover slides onto the crystallization plate.

Each Cover Slide Gizmo includes a single cover. Manual operation, no power required. See the Glass Cover Slide Gizmo Dispenser at work at minute 1:06.

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Hampton蛋白结晶试剂盒Glass Cover Slide Gizmo Dispenser

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Hampton蛋白结晶试剂盒Glass Cover Slide Gizmo Dispenser
Hampton蛋白结晶试剂盒Glass Cover Slide Gizmo Dispenser
Hampton蛋白结晶试剂盒Glass Cover Slide Gizmo Dispenser
Hampton蛋白结晶试剂盒Glass Cover Slide Gizmo Dispenser
Hampton蛋白结晶试剂盒Glass Cover Slide Gizmo Dispenser

CAT NO

HR8-167

NAME

Cover Slide Gizmo

DESCRIPTION

Dispenser for 12 x 0.22 mm circle cover slides

PRICE

$170.00

cart quote

CAT NO

HR8-168

NAME

Cover Slide Gizmo

DESCRIPTION

Dispenser for 12 x 0.96 mm circle cover slides

PRICE

$170.00

cart quote

CAT NO

HR8-169

NAME

Cover Slide Gizmo

DESCRIPTION

Dispenser for 18 x 0.22 mm circle cover slides

PRICE

$170.00

cart quote

CAT NO

HR8-170

NAME

Cover Slide Gizmo

DESCRIPTION

Dispenser for 18 x 0.96 mm circle cover slides

PRICE

$170.00

cart quote

CAT NO

HR8-162

NAME

Cover Slide Gizmo

DESCRIPTION

Dispenser for 22 x 0.22 mm circle cover slides

PRICE

$170.00

cart quote

CAT NO

HR8-163

NAME

Cover Slide Gizmo

DESCRIPTION

Dispenser for 22 x 0.96 mm Circle Cover Slides

PRICE

$170.00

Temporarily Unavailable
More Available Soon!

CAT NO

HR8-164

NAME

Cover Slide Gizmo

DESCRIPTION

Dispenser for 22 x 0.22 mm square cover slides

PRICE

$170.00

Temporarily Unavailable
More Available Soon!

CAT NO

HR8-165

NAME

Cover Slide Gizmo

DESCRIPTION

Dispenser for 22 x 0.96 mm square cover slides

PRICE

$170.00

cart quote

Support Material(s)

Hampton蛋白结晶试剂盒Glass Cover Slide Gizmo Dispenser HR8-167 12×0.22mm Circle Dispenser User GuideHampton蛋白结晶试剂盒Glass Cover Slide Gizmo Dispenser HR8-168 12x1mm Circle Dispenser User GuideHampton蛋白结晶试剂盒Glass Cover Slide Gizmo Dispenser HR8-169 18×0.22mm Circle Dispenser User GuideHampton蛋白结晶试剂盒Glass Cover Slide Gizmo Dispenser HR8-170 18x1mm Circle Dispenser User GuideHampton蛋白结晶试剂盒Glass Cover Slide Gizmo Dispenser HR8-162 22×0.22nn Circle Dispenser User GuideHampton蛋白结晶试剂盒Glass Cover Slide Gizmo Dispenser HR8-163 22x1mm Circle Dispenser User GuideHampton蛋白结晶试剂盒Glass Cover Slide Gizmo Dispenser HR8-164 22×0.22mm Square Dispenser User GuideHampton蛋白结晶试剂盒Glass Cover Slide Gizmo Dispenser HR8-165 22x1mm Square Dispenser User Guide

Related Item(S)

  • 22 mm Siliconized Glass Cover Slides
  • 18 mm Siliconized Glass Cover Slides
  • 12 mm Siliconized Glass Cover Slides

References

 

Yatalase酶试剂盒Takara


Yatalase
品牌 Code No. 产品名称 包装量 价格(元) 说明书 数量
OZK T017 Yatalase 2 g ¥2,535 Yatalase Yatalase Yatalase
收藏产品 加入购物车
 
■ 制品说明
本制品是从Corynebacterium sp. OZ-21培养上清液中制备而来的复合酶。
◇ 具有良好的热稳定性
◇ 具有Chitinase、Chitobiase酶活性
◇ 可制备丝状真菌的原生质体
对于一些难处理的黑曲霉等丝状真菌,建议使用Yatalase -Plus-(Code No. T030)
 
■ 保存
4℃,干燥条件。
 
■ 形态
干粉(含乳糖作为辅料)
 
■ 酶活性
Chitinase活性: 50 U/g powder 以上
Chitobiase活性:500 U/g powder 以上
细胞壁溶解活性: 约10,000 U/g powder
 
■ 起源
Corynebacterium sp. OZ-21
 
■ Chitinase活性测定
活性定义:在下述条件下,1分钟释放1 μmol的游离N –Acetylglucosamine所需要的酶量定义为1 U。
测定方法:
试剂A:醋酸缓冲液
  在90 ml 蒸馏水中溶解0.82 g的醋酸钠。在溶液中逐次少量加入醋酸调节pH至6.0。定容至100 ml,该溶液即为0.1 M醋酸缓冲液。
试剂B:基质液
  在A液中加入0.5%的Chitin制成基质液。
试剂C:酶液
  加蒸馏水溶解使其终浓度为0.8 mg/ml。
操作步骤
1. 在L型实验管中加入1 ml 试剂B,37℃孵育5~10分钟。
2. 添加0.2 ml试剂C后,37℃轻柔振荡反应1小时。
3. 加热失活(煮沸5分钟)后,3,000 rpm离心10分钟,通过Reissig法测定上清中的N-Acetylglucosamine的量。
4. 通过标准曲线计算出酶反应释放的游离N-Acetylglucosamine量。
 
■ Chitobiase活性测定
活性定义:在下述条件下,1分钟释放1 μmol游离的p-Nitrophenol所需的酶活性定义为1 U。
测定方法:
试剂A:MclLvaine缓冲液
  0.1 M柠檬酸(柠檬酸2.1 g溶解于蒸馏水中定容至100 ml)中加入0.2 M磷酸氢二钠溶液(十二水合磷酸氢二钠10.74 g溶解于蒸馏水中定容至150 ml)并调整pH为6.0。
试剂B:基质液
  将8.56 mg p-Nitrophenyl-N-acetyl-β-D-glucosaminide 添加到5.0 ml A液中使其溶解。
试剂C:酶液
  加蒸馏水溶解使其终浓度为0.8 mg/ml。
试剂D:反应停止液
  碳酸钠(无水)2.65 g 加蒸馏水溶解后定容至100 ml。
 
操作步骤
1. L型反应管中添加0.75 ml试剂A和0.2 ml试剂B,37℃孵育5~10分钟。
2. 加入50 μl试剂C,迅速将反应管混匀后开始反应。
3. 10分钟轻柔振荡后,添加2.0 ml试剂D,迅速混匀后,在405 nm测定吸光度。
4. 通过标准曲线计算酶反应释放出的游离p-Nitrophenol的量。
 
■ 细胞壁溶解活性测定
活性定义:在下述条件下,1小时内引起溶液于660 nm处吸光度减少1%时所需的酶量定义为1 U。
测定方法:
试剂A:基质液
  收集Aspergillus oryzae菌体(Dextrin-Peptone培养基,25~30℃培养1-2天)并使用Waring搅拌器匀浆(16,000 rpm,3 min)。之后使用French press(弗氏压碎器)将菌体压成碎片,在滤纸上充分水洗后,再使用丙酮和乙醚洗涤。风干沉淀后添加0.1 M醋酸缓冲液(pH6.0)将其溶解成终浓度为0.125%(w/v)的悬浊液,此为基质液。
试剂B:酶液
  加蒸馏水溶解使其终浓度为0.8 mg/ml。
 
操作步骤
1. 反应管中加入4 ml试剂A。
2. 添加1 ml 试剂B后,迅速混匀,于37℃轻柔振荡培养1小时。
3. 迅速检测660 nm波长下溶液的吸光度。
 
■ 一般性质
最适pH : pH7.2
最适温度 : 37℃
 
Yatalase
 
表1:各种酶活性
活性(U/g powder)
Chitinase >50
Chitobiase >500
Chitosanase* 19
β-1,3-Glucanase* 300
Protease* 31
细胞壁溶解活性 10,000
* Chitosanase、β-1,3-Glucanase、Protease的值为代表性的样品值,不是标准值。
 
本制品由Ozeki corporation制造。
 
 

页面更新:2020-10-20 16:34:19

日本同仁化学Hampton蛋白结晶试剂盒Siliconized Glass Coverslips (Greiner)- DOJINDO

上海金畔生物科技有限公司代理日本同仁化学 DOJINDO代理商全线产品,欢迎访问官网了解更多信息

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等,我们将竭诚为您服务,欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。Products > Crystallization Plates, Hardware & Accessories > Cover Slides & Related Tools > Siliconized Glass Coverslips (Greiner)

Siliconized Glass Coverslips (Greiner)

Applications

  • Hanging or sitting drop vapor diffusion crystallization

Features

  • High purity of glass
  • Siliconized, hydrophobic glass surface
  • 0.19-0.22mm glass thickness
  • 18 & 22 mm glass diameter
  • Circle glass cover slip
  • UV imaging compatible (>85% transmission)

Description

Siliconized glass coverslips allow a droplet to be suspended in a position which provides near optimal conditions for vapor diffusion with the surrounding reservoir solution. These siliconized glass cover slides provide a consistent, high quality finish for crystallization experiments. The hydrophobic surface produces a drop which “stands well” and does not flatten on the glass. The siliconized surface prevents the adhesion of crystals and precipitate onto the glass surface. Use vacuum grease or oil to seal cover slide to plate. Available in 18 or 22 mm diameter circles.

HR3-254 is Greiner Bio-One 503870 siliconized glass coverslip, round, 22mm diameter, 0.19-0.22mm thick. Non-sterile. 100 pieces per inner pack. Ten inner packs, totaling 1,000 pieces per packing unit. Fit VDX Plate.

HR3-255 is Greiner Bio-One 501870 siliconized glass coverslip, round, 18mm diameter, 0.19-0.22mm thick. Non-sterile. 100 pieces per inner pack. Ten inner packs, totaling 1,000 pieces per packing unit. Fits VDXm Plate and ComboPlate (Greiner 662050 and 662150).

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Hampton蛋白结晶试剂盒Siliconized Glass Coverslips (Greiner)

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Hampton蛋白结晶试剂盒Siliconized Glass Coverslips (Greiner)
Hampton蛋白结晶试剂盒Siliconized Glass Coverslips (Greiner)

CAT NO

HR3-254

NAME

22mm Siliconized Circle Coverslips

DESCRIPTION

Pack (10×100 = 1,000)

PRICE

$426.00

cart quote

CAT NO

HR3-255

NAME

18mm Siliconized Circle Coverslips

DESCRIPTION

Pack (10×100 = 1,000)

PRICE

$393.00

cart quote

Westase™酶试剂盒Takara


Westase
品牌 Code No. 产品名称 包装量 价格(元) 说明书 数量
Takara 9005 Westase 1 g ¥689 Westase™ Westase™ Westase™
收藏产品 加入购物车

无标题文档

 
■ 制品形态 Westase™
干粉 (含硅藻土作为辅料) 。
 
■ 制品说明
本制品由Streptomyces rochei DB-34培养上清液中制备而来。本制品主要包含了β-1,6葡聚糖酶和β-1,3葡聚糖酶,是用于溶解酵母细胞壁的混合酶。
 
■ 保存
4℃,干燥条件。
 
■ 起源
Streptomyces rochei DB-34
 
■ 酶溶液的制备
1. 溶解于Mcllvain Buffer*
* Mcllvain Buffer由0.1 M柠檬酸和0.2 M磷酸氢二钠以36.8 : 63.2体积比配制而成,调整pH为6.0。
2. 使用醋酸纤维素滤膜对溶液进行过滤。
 
■ 注意事项
1. 使用Westase制备原生质体时,一定要使用酒石酸钠 (0.3-0.5 M) 作为渗透压稳定剂。使用山梨糖醇作为稳定剂会明显降低原生质体形成的效率。
2. 使用Westase制备原生质体时,选择指数生长期的酵母细胞比较适合。在大多数酵母菌株中,当酵母细胞处于稳定期时,原生质体形成效率会显著降低。
3. 使原生质体细胞壁再生时,可使用1.0-1.5 M山梨醇作为渗透压稳定剂。
 
 

页面更新:2019-11-26 11:35:31

RNase-free Water酶试剂盒Takara


RNase-free Water
品牌 Code No. 产品名称 包装量 价格(元) 说明书 数量
Takara 9012 RNase-free Water 1 ml × 10 ¥65
¥55
RNase-free Water RNase-free Water RNase-free Water

*红字为促销价格,促销时间: 2022年9月1日-2022年10月31日

收藏产品 加入购物车
 
■ 制品内容
RNase-free Water 1 ml × 10 支
 
■ 制品说明
本制品是非焦碳酸二乙酯 (Diethylpyrocarbonate;DEPC) 处理的RNase Free水。因此,不必担心有DEPC残留对RT-PCR反应的阻害作用。适用于RNA样品的制备、反应液配制等。
经检测可确认本产品不含DNase。
 
■ 保存
-20℃
 
 

页面更新:2019-06-26 09:17:15