iMatrix-511

iMatrix-511 silk

生产系统

转基因CHO-S细胞

转基因蚕生产系统

提纯材料

CHO-S细胞培养上清

蚕蛹蛋白

产品等级

实验研究用* *有临床用级别

实验研究用

导入基因

人层粘连蛋白511-E8片段

纯度

95%以上

浓度

0.5mg/mL

解离常数

10nM以下

使用期限

生产后2年内

iPS细胞培养能力

添加0.5μg/cm²到培养容器中，可用于iPS细胞的维持培养

产品编号

生产商编号

产品名称

包装

385-07361

892011

iMatrix-511 solution(0.5mg/mL)

层粘连蛋白511-E8片段，

溶液（0.5mg/mL）

175µg×2（350µL×2)

381-07363

892012

175µg×6（350µL×6)

产品

保质期

iMatrix-511

自生产后两年内

iMatrix-511silk

自生产后两年内

iMatrix-511MG

自生产后两年内

分类

文献信息

主题

人多能干细胞（hPSC）的确立

Miyazaki et al. Nat. Commun.3:1236, (2012)

证实用作hPSC的培养基质的有效性

Nakagawa et al. Sci. Rep. 4:3594, (2014)

确立医疗等级的hPSC

Takashima et al. Cell.158(6):1254-69, (2014)

促进向hPSC的基质状态的转移

Miyazaki et al. Sci. Rep.7:41165, (2017)

采用无需涂层操作的添加法培养hPSC

Sekine et al. Stem Cell Res.24:40-43, (2017)

确立疾病特异性的hPSC

Tan et al. Stem Cell Res. 24:12-15, (2017)

Ishida et al. Sci. Rep. 8(1), 310, (2018)

利用hPSC的基因编辑建立遗传性疾病模型

Kim et al. Nature Communications, 9(1), 939, (2018)

Sakai-Takemura et al. Sci. Rep, 8, 6555, (2018)

悬浮培养由hPSC分化的肌肉前体细胞

由hPSC分化衍生的细胞

Doi et al. Stem Cell Reports. 2(3):337-50, (2014)

多巴胺产生神经元

Ishikawa et al. Hum. Mol. Genet.25(23):

5188-5197, (2016)

Nishimura et al. Stem Cell Reports.6(4):

511-524, (2016)

Samata et al. Nat. Commun. 7:13097, (2016)

Kikuchi et al. Nature. 548(7669):592-596, 　　(2017)

Morizane et al. Nat. Commun.8(1):385, (2017)

Kikuchi et al. J. Neurosci. Res.95(9):1829-37, (2017)

Goparaju et al. Sci. Rep. 7:42367, (2017)

运动神经元

Burridge et al. Nat. Methods.11(8):855-60, 　  (2014)

心肌细胞

Sougawa et al. Sci. Rep,8(1), 3726, (2018)

Yamauchi et al. BBRC, 495(1), 1278-1284, 　    (2018)

心室肌细胞

Akiyama et al. Sci. Rep, 8(1), 1189, (2018)

骨骼肌细胞

Saito et al. Stem Cell Res Ther, 9(1), 12, (2018)

成骨细胞

Uchimura et al. Stem cell research, 25, 98-106, (2017)

成肌细胞

Hayashi et al. Nature.531(7594):376-80, (2016)

视觉细胞

Hayashi et al. Nat. Protoc.12(4):683-696, (2017)

角膜上皮细胞

Takayama et al. BBRC. 474(1):91-96, (2016)

胆管上皮细胞

Takayama et al. Hepatol Commun,1(10), 1058-1069, (2017)

肝实质细胞

Takayama et al. Biomaterials, (2018)

Takebe et al. Cell Reports, 21(10), 2661-2670, (2017)

肝细胞

Tan et al. Stem Cell Reports, 11:1-11, (2018)

Camp et al. Nature. 546(7659):533-38, (2017)

定形内胚层细胞

Zhang et al. Stem Cell Reports, 10(2), 1–14, (2018)

后内胚层前体细胞

Tanigawa et al. Cell reports, 15(4), 801-813, (2016)

肾单位前体细胞（胎肾细胞）

Musah et al. Nat.Biomed.Eng.1:0069, (2017)

肾小球上皮细胞

Musah et al. Nature protocols,13(7):1662, 　  (2018)

Mae et al. BBRC, 495(1), 954-961, (2018)

输尿管芽组织

Oshima et al. BBRC, 497(2), 719-725, (2018)

血细胞・血管内皮常见前体细胞

Taguchi et al. Cell Stem Cell, 21, (2017) 

*培养hPSC用于分化肾单位前体细胞（胎肾细胞）

Kawamura et al. Stem Cell Reports.6(3):312-20,(2016)

*培养hPSC用于分化心肌细胞

Sasaki et al. Cell Stem Cell.17(2):178-94, (2015)

*培养hPSC用于分化生殖细胞

Kojima et al. Cell Stem Cell.21(4):517-532, 　  (2017)

Furuta et al. PLoS One. 9(12):e112291, (2014)

*培养hPSC用于分化间充质细胞

人原代细胞的培养

Okumura et al. Invest. Ophth. Vis. Sci.56(5):2933-42, (2015)

人角膜内皮细胞

Hongo et al. Invest. Ophth. Vis. Sci.58(9):3325-34, (2017)

Polisetti et al. Sci. Rep.7(1):5152, (2017)

人角膜边缘上皮前体细胞

Ishii et al. Stem Cell Reports, 10, 1-15, (2018)

卫星细胞

层粘连蛋白-整合素相互作用的分子机制

Ido et al. J. Biol. Chem. 282(15): 11144-54, 　  (2007)

Ido et al. J. Biol. Chem.283(42): 28149-57, 　  (2008)

Taniguchi et al. J. Biol. Chem. 284(12): 7820-31, (2009)

Taniguchi et al. BBRC.487(3): 525-531, (2017)

Takizawa et al. Sci Adv.3(9) :e1701497, (2017)

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【起源】
　Clostridium histolyticum
【规格】
　外观：褐色、结晶性粉末~粉末
　内毒素：实际值报告
　活菌数测试：1cfu/ml 以下
　活性：125units/mg以上
　酪蛋白酶活性：200units/mg以上
　梭菌蛋白酶活性 :4.0units/mg以下
　胰蛋白酶活性：0.5units/mg 以下

【起源】
　Clostridium histolyticum
【规格】
　外观：褐色、结晶性粉末～粉末
　内毒素：实际值报告
　活菌数测试：1cfu/ml 以下
　活性：125units/mg以上
　酪蛋白酶活性：200units/mg以上
　梭菌蛋白酶活性：3.5units/mg以上
　胰蛋白酶活性：0.1units/mg 以上

产品编号

品名

规格

容量

起源

胶原酶活性

酪蛋白酶活性

梭菌蛋白酶活性

胰蛋白酶活性

蛋白质浓度

活菌数测试

内毒素

038-22361

Collagenase

胶原酶

细胞分散用

100mg

Clostridium

histolyticum

实测值

标签显示

–

–

–

–

–

–

034-22363

1g

032-22364

5g

031-17601

Collagenase   Type I

胶原酶 I型

细胞分散用

100mg

Clostridium

histolyticum

实测值

标签显示

–

–

–

–

–

–

037-17603

500mg

035-17604

1g

038-17851

Collagenase   Type V

胶原酶V型

细胞分散用

100mg

Clostridium

histolyticum

实测值

标签显示

–

–

–

–

–

–

032-17854

1g

035-17861

Collagenase   Type X

胶原酶X型

细胞分散用

100mg

Clostridium

histolyticum

实测值

标签显示

–

–

–

–

–

–

031-17863

500mg

039-17864

1g

034-13291

Collagenase

胶原酶

胶原分析用

25mg

Clostridium

histolyticum

1,000units
  /mg以上
  （曼德尔法）

–

–

–

–

–

–

031-22591

Collagenase   Type I, Filtered

胶原酶 I型，已过滤

细胞分散用

50mg

Clostridium

histolyticum

125units
  /mg以上
  (实测值

标签显示)

200units
  /mg以上

4units
  /mg以下

0.5units
  /mg以下

–

1cfu
  /ml以下

–

038-23961

Collagenase   Type X, Filtered

胶原酶 X型，已过滤

细胞分散用

50mg

Clostridium

histolyticum

125units
  /mg以上
  (实测值

标签显示)

200units
  /mg以上

3.5units
  /mg以上

0.1units
  /mg以上

–

1cfu
  /ml以下

–

036-23141

Collagenase,   recombinant, Animal-derived-free

胶原酶，重组，无动物源性成分

细胞分散用

240,000
  units

Brevibacillus

choshinensis   expressed Grimontiahollisae collagenase

6,000units
  /mg以上
  (实测值

标签显示)

–

–

–

85%
  以上

(已消毒)

10EU
  /mg以下