Phos-tag™ SDS-PAGE相关产品
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Phos-tag™ SDS-PAGE相关产品
◆Reagents for Phos-tag™ SDS-PAGE gel preparation
Product Name |
Pkg. Size |
Wako Cat. No. |
Use Application |
30 w/v% Acrylamide/Bis Mixed solution(29:1) |
500 mL |
015-25635 |
Ready-to-use“Solution A”.30%T, 3.3%C |
Agarose H(High-strength type) |
1 g |
315-01203 |
High-strength Agarose has high strength even in a low-agarose environment and is suitable for electrophoretic migration of large nucleic acid fragments. It can be used in a concentration range of 0.2 – 1% and a separation range of 1 – 200 kbp. |
10 g |
319-01201 |
||
25 g |
317-01202 |
||
10% SDS Solution |
100 mL |
311-90271 |
Ready-to-Use“Solution D” |
500 mL |
313-90275 |
||
Manganese(Ⅱ) Chloride Tetrahydrate, |
25 g |
134-15302 |
for Molecular Biology Please use for preparation of“Solution F” |
100 g |
136-15301 |
||
Zinc Chloride |
25 g |
268-01902 |
for Molecular Biology. Please use for preparation of“Solution M” |
Bis-Tris |
100 g |
345-04741 |
Please use for preparation of“Solution N” |
Sodium Hydrogensulfite〈JIS Special Grade〉 |
25 g |
196-01372 |
Please use for preparation of“Solution O” |
100 g |
198-01371 |
||
Sodium Hydrogensulfite |
100 g |
190-16461 |
|
MOPS |
100 g |
345-01804 |
Please use for preparation of“Solution P” |
250 g |
341-01801 |
||
MOPS〈For Molecular Biology〉 |
100 g |
341-08241 |
|
500 g |
343-08245 |
||
Separating Gel Buffer Solution (x4) |
250 mL |
192-11041 |
Ready-to-Use mixed solution of“Sol. B”and“Sol. D”for preparation of Resolving Gel. Contains SDS. |
Stacking Gel Buffer Solution (x4) |
250 mL |
199-11051 |
Ready-to-Use mixed solution of“Sol.C”and“Sol. D”for preparation of Stacking Gel. Contains SDS. |
N,N,N',N'-Tetramethylethylenediamine (TEMED) |
25 mL |
205-06313 |
for Electrophoresis |
10 w/v% Ammonium Peroxodisulfate Solution |
25 mL |
019-15922 |
Ready-to-Use“Solution G” |
◆Premixed Buffers
Product Name |
Pkg. Size |
Wako Cat. No. |
Use Application |
Running Buffer Solution (x10) |
1 L |
184-01291 |
Ready-to-Use concentrated“Solution H” |
SDS-PAGE 10x Running Buffer |
1 L |
312-90321 |
Ready-to-Use concentrated“Solution H” |
5 L |
318-90323 |
||
SDS-PAGE Buffer, pH 8.5 |
5 L |
192-16801 |
Ready-to-Use“Solution H”, 1×buffer. |
Tricine Running Buffer Solution (×10) |
1 L |
200-17071 |
Composition: 0.5 M Tris / 0.5 M Tricine/1% SDS |
Sample Buffer Solution (2ME+) (×2) |
25 mL |
196-11022 |
Sample buffer for Laemmli SDS-PAGE containing 2-mercaptoethanol |
Sample Buffer Solution |
25 mL |
199-16132 |
Laemmli Sample Buffer containing 3-mercapto-1,2-propanediol (non-hazardous chemical) as substitute for 2-ME |
Sample Buffer Solution |
25 mL |
196-16142 |
|
AquaBlot™ 10× Tris-Glycine-SDS Transfer |
1 L |
019-25111 |
Please use it to transfer to a membrane. Containing 0.05% SDS |
◆Enzyme for Dephosphorylation
Product Name |
Pkg. Size |
Wako Cat. No. |
Use Application |
Alkaline Phosphatase(for Biochemistry) |
50 U |
018-10693 |
Applicable to dephosphorylation of proteins |
100 U |
012-10691 |
◆Reagents for Staining
Product Name |
Pkg. Size |
Wako Cat. No. |
Use Application |
Quick CBB Plus |
250 mL |
174-00553 |
Ready-to-Use Sol. K. |
1 L |
178-00551 |
||
Quick-CBB |
2 L |
299-50101 |
By mixing staing solution A and B, ready-to-Use Sol. K |
Silver Stain MS Kit |
20 tests |
299-58901 |
Modified proteins by glycosilation and / or phosphorylation can be detected sub-nanogram level on the electrophoretic gel. |
Silver Stain Kit Wako |
for10 gels |
299-13841 |
50~100 times more sensitive than CBB method. |
Silver Stain Ⅱ Kit Wako |
for10 gels |
291-50301 |
This kit contains Stopper, which can be adjusted the staining strength. |
Negative Gel Stain MS Kit |
20 tests |
293-57701 |
Applicable to mass analysis and Western blot |
◆Protein Size Marker
Product Name |
Pkg. Size |
Wako Cat. No. |
Use Application |
WIDE-VIEW™ Prestained Protein Size Marker Ⅲ |
25 μL |
236-02463 |
A recommendable prestained marker used with Phos-tag™ SDS-PAGE because obtained bands are less distorted. |
500 μL |
230-02461 |
||
500 μL x 3 |
234-02464 |
◆Positive Control(for confirmation of separation capacity of Phos-tag™ SDS-PAGE)
Product Name |
Pkg. Size |
Wako Cat. No. |
Use Application |
α-Casein, from BovineMilk, Dephosphorylated |
1 mg |
038-23221 |
Mixture of phosphylated and dephosphorylated α-casein |
10 mg |
034-23223 |
◆Electrophoresis Apparatus ・ Precast Gels
Product Name |
Pkg. Size |
Wako Cat. No. |
Use Application |
EasySeparator |
1 unit |
058-07681 |
An electrophoresis tank for SuperSep precast polyacrylamide gels. |
SuperSep Ace, 6%, 13 wells |
10 gels |
195-15171 |
Prior to use of SuperSep Phos-tag™ PAGE, please use these as sample confirmation. |
SuperSep Ace, 7.5%, 13 wells |
10 gels |
198-14941 |
|
SuperSep Ace, 7.5%, 17 wells |
10 gels |
191-14931 |
|
SuperSep Ace, 10%, 13 wells |
10 gels |
195-14951 |
|
SuperSep Ace, 10%, 17 wells |
10 gels |
192-14961 |
|
SuperSep Ace, 12.5%, 13 wells |
10 gels |
199-14971 |
|
SuperSep Ace, 12.5%, 17 wells |
10 gels |
196-14981 |
|
SuperSep Ace, 15%, 13 wells |
10 gels |
193-14991 |
|
SuperSep Ace, 15%, 17 wells |
10 gels |
190-15001 |
◆Reagents for Western Blotting
Product Name |
Pkg. Size |
Wako Cat. No. |
Use Application |
ImmunoStar LD* |
200 cm2 |
296-69901 |
Highly sensitive (femto gram level) immunoblotting, utilizing detection by enhanced chemiluminescence using a unique luminol derivative L-012 as a substrate. |
1,000 cm2 |
292-69903 |
||
2,000 cm2 |
290-69904 |
||
ImmunoStar Zeta* |
200 cm2 |
291-72401 |
Use for detection of proteins between the middle and low femto gram levels. Has stable, long-lasting luminescence signal. |
1,000 cm2 |
297-72403 |
||
2,000 cm2 |
295-72404 |
||
ImmunoStar Basic* |
200 cm2 |
295-75101 |
A cost-effective, stable and long-lasting product. |
2,000 cm2 |
291-75103 |
||
5,000 cm2 |
299-75104 |
||
Immuno Enhancer |
2 assays |
294-68601 |
Ready-to-Use Immunoreaction Enhancer for Western blotting and ELISA |
10 assays |
290-68603 |
||
40 assays |
298-68604 |
* : Not available for sales in the US and Europe.