日本同仁化学Hampton蛋白结晶试剂盒NDSB-256-4T/HR2-794- DOJINDO

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Products > Optimize Reagents > Optimize – Solubilizing Agents (NDSB) > NDSB-256-4T

NDSB-256-4T

Hampton蛋白结晶试剂盒NDSB-256-4T/HR2-794

CAT NO

HR2-794

NAME

NDSB-256-4T

DESCRIPTION

5 grams

PRICE

$172.00

支持材料

Hampton蛋白结晶试剂盒NDSB-256-4T/HR2-794 HR2-794 NDSB-256-4T User GuideHampton蛋白结晶试剂盒NDSB-256-4T/HR2-794 HR2-794 NDSB-256-4T SDS

应用

  • Crystallization grade NDSB-256-4T for formulating screens or for optimization
  • 结晶级 NDSB-256-4T 用于配制筛选或优化

特征

  • Efficiently solubilizes proteins at non-denaturing concentrations
  • Useful in preventing protein aggregation
  • Non detergent sulfobetaines (NDSB) are used for protein folding, renaturation and crystallization
  • Protein specific additive for solubility and stability
  • NDSB 256 4T is a non-detergent sulfobetaine (NDSB) that prevents protein aggregation and facilitates protein folding by interacting with the early folding intermediates. Reported to improve the in vitro renaturation efficiency of reduced hen egg lysozyme (60% enzymatic activity at 600 mM NDSB-256-4T) and chemically unfolded tryptophan synthase ß2 subunit (100% enzymatic activity at 1 M NDSB-256-4T).
  • 在非变性浓度下有效溶解蛋白质
    可用于防止蛋白质聚集
    非洗涤剂磺基甜菜碱 (NDSB) 用于蛋白质折叠、复性和结晶
    用于溶解性和稳定性的蛋白质特定添加剂
    NDSB 256 4T 是一种非去污剂磺基甜菜碱 (NDSB)，可通过与早期折叠中间体相互作用来防止蛋白质聚集并促进蛋白质折叠。 据报道可提高还原鸡蛋溶菌酶（在 600 mM NDSB-256-4T 下的酶活性为 60%）和化学展开的色氨酸合酶 ß2 亚基（在 1 M NDSB-256-4T 下的酶活性为 100%）的体外复性效率。

描述

The NDSB are a group of zwitterionic compounds that can reduce aggregation and aid in refolding proteins found in inclusion bodies and bacterial expression systems.

Non detergent sulfobetaines have a sulfobetaine hydrophilic group and a short hydrophobic group that cannot aggregate to form micelles, therefore NDSB’s are not considered detergents. NDSB increase the extraction yield (up to 30%) of membrane, nuclear and cyto-skeletal associated proteins. The short hydrophobic groups combined with the charge neutralization of the sulfobetaine group results in higher yields of membrane proteins. NDSB have been used in refolding and renaturation of chemically and thermally denatured proteins.

NDSB 是一组两性离子化合物，可以减少聚集并帮助重新折叠包涵体和细菌表达系统中发现的蛋白质。

非洗涤剂磺基甜菜碱有一个磺基甜菜碱亲水基团和一个不能聚集形成胶束的短疏水基团，因此 NDSB 不被视为洗涤剂。 NDSB 可提高膜、核和细胞骨架相关蛋白的提取率（高达 30%）。 短疏水基团与磺基甜菜碱基团的电荷中和相结合，导致膜蛋白产量更高。 NDSB 已用于化学和热变性蛋白质的重折叠和复性。

Typical useful NDSB concentration in protein sample is 0.5-1.0 M.

NDSB-256-4T
Synonym: 3-(4-tert-Butyl-1-pyridino)-1-propane sulfonate, SB256-4T, SN2564T
C12H19NO3S
CAS Number [570412-84-9]
Mr 257.35
Purity = 98% by HPLC
Hygroscopic
Store at room temperature

参考NDSB-256-4T

1. Expert-Bezancon, N., et. al., Biophys Chem, 100, 469 (2003).