日本同仁化学BAPTA-AM试剂货号:B018 CAS号:126150-97-8- DOJINDO

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BAPTA-AM试剂货号:B018
O,O’-Bis(2-aminophenyl)ethyleneglycol-N,N,N’,N’-tetraacetic acid, tetraacetoxymethyl ester
CAS号:126150-97-8
商品信息
储存条件:-20度保存
运输条件:室温
分子式:

C34H40N2O18

分子量:

764.68

特点:

 

● 螯合率高于EGTA

● 可螯合细胞内的钙离子

SDS下载

选择规格:
25mg

现货 

 
荧光探针检测方案

性质
参考文献
规格性状

性质

BAPTA-AM是BAPTA羧基的乙酰氧基甲基酯化产物,可以很容易地吸收到细胞中。 该酯被细胞内酯酶分解,显示出主体在细胞中作为BAPTA的功能。 生成的BAPTA保留在细胞内部。

参考文献

1) R. Y. Tsien, “New Calcium Indicators and Buffers with High Selectivity against Magnesium and Protons: Design, Synthesis, and Properties of Prototype Structures”, Biochemistry198019 (11), 2396.
2) R. Y. Tsien, “A Non-disruptive Technique for Loading Calcium Buffers and Indicators into Cells”, Nature1981290, 527.
3) J. I. Korenbrot, D. L. Ochs, J. A. Williams, D. L. Miller and J. E. Brown, “The Use of Tetracarboxylate Fluorescent Indicators in the Measurement and Control of Intracellular Free Calcium Ions”, Soc. Gen. Physiol. Ser.198640, 347.
4) S. M. Harrison and D. M. Bers, “The Effect of Temperature and Ionic Strength on the Apparent Ca-affinity of EGTA and the Analogous Ca-chelators BAPTA and Dibromo-BAPTA”, Biochim. Biophys. Acta1987925, 133.
5) E. W. Gelfand and R. K. Cheung, “Dissociation of Unidirectional Influx of External Ca2+ and Release from Internal Stores in Activated Human T Lymphocytes”, Eur. J. Immunol.199020, 1237.
6) J. P. Kao, J. M. Alderton, R. Y. Tsien and R. A. Steinhardt, “Active Involvement of Ca2+ in Mitotic Progression of Swiss 3T3 Fibroblasts”, J. Cell Biol.1990111, 183.
7) M. L. Schubert and J. Hightower, “Functionally Distinct Muscarinic Receptors on Gastric Somatostatin Cells”, Am. J. Physiol.1990258, G982.
8) Y. Tojyo and Y. Matsumoto, “Inhibitory Effects of Loading with the Calcium-chelator 1, 2-Bis(o-aminophenoxy)ethane-N,N,N’,N’-tetraacetic acid (BAPTA) on Amylase Release and Cellular ATP Level in Rat Parotid Cells”, Biochem. Pharmacol.199039(11), 1775.

规格性状

规格性状:

该产品为无色液体。

纯度(HPLC):98.0%以上

处理条件

1.危险物第4类,第3石油类,危险等级Ⅲ 2.无火 3.储存方法:冷冻,避光