STEMCELL淋巴细胞分离液Lymphoprep
- 产品型号: 07851
- 简单描述
- STEMCELL淋巴细胞分离液LymphoprepLymphoprep分离液Lymphoprep是一种无菌、内毒素含量低的即用型分离液,是目前国内外分离正常人或病人外周血单个核细胞(PBMC:Mononuclear cells of Peripheral blood)(包括单核细胞monocytes和淋巴细胞lymphocytes)的常用方法,具有与昂贵的GE公司的PLUS或PREMIUM分
STEMCELL淋巴细胞分离液Lymphoprep
*替代Ficoll-Paque
工作原理
PBMC与血液中的其他成分存在密度差异,利用密度在1.077±0.001g/ml之间而且近于等渗的LymphoprepTM溶液作密度梯度离心时,红细胞和粒细胞由于密度大,故沉于分离液底部。PBMC密度稍低于分离液,故位于分离液界面上,获得PBMC。LymphoprepTM分离液含有多糖(polysacccharide),多糖能粘附在细胞表面,使红细胞和粒细胞聚集下沉,但它对淋巴细胞的活性和红细胞和粒细胞进一步分离有负面影响。
STEMCELL淋巴细胞分离液Lymphoprep
注意事项:
1、该分离液仅适用于人体的外周血液,不适用于动物。
2、使用该分离液时,需将待分离的血液用生理盐水做等体积的稀释。
3、分离液的保存应置于无菌环境,并避光保存。
4、详细操作步骤见产品说明书。
技术参数
成分:泛影葡胺钠(Sodium diatrizoate):9.1% (w/v);多糖:5.7% (w/v)
密度:1.077±0.001g/ml
渗透性:290±15mOsm
内毒素含量:<1.0EU/ml
分离所得PBMC中红细胞的含量:整个细胞数目的3-10%。
Lymphoprep™ is a density gradient medium recommended for the isolation of mononuclear cells from peripheral blood, cord blood, and bone marrow by exploiting differences in cell density. Granulocytes and erythrocytes have a higher density than mononuclear cells and therefore sediment through the Lymphoprep™ layer during centrifugation. Lymphoprep can be substituted for Ficoll-Paque™ without any need to change your existing protocols and is fully compatible with both Sepmate™ and RosetteSep™.
Product Type: | Cell isolation products |
Recommended For: |
Isolation of mononuclear cells from peripheral blood, cord blood, and bone marrow |
Components: |
• Sodium Diatrizoate 9.1% (w/v) • Polysaccharide 5.7% (w/v) • Water • Density of 1.077 g/mL |
Accessory Products: |
SepMate™-50 (Catalog #15450/15460) |
Contains: |
• Sodium Diatrizoate (9.1% w/v) • Polysaccharide (5.7% w/v) • Density of 1.077 g/mL |
Equipment Required: |
Centrifuge |
Cell Isolation Product Type: | Reagents |
Cell Source: | Bone marrow, Cord blood, Whole blood |
Legal Statement: | Lymphoprep™ is a trademark of Axis-Shield. |
Intended Use Statement: | FOR RESEARCH USE ONLY. NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES. |
ISO Statement: | STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485 MEDICAL DEVICE STANDARDS. |
订购信息:
07851 500ml
07861 6*500ml
现货*!