双染蛋白MARKERPrecision Plus Protein™ Dual Color Stand
- 产品型号: BIO-RAD代理 1610374
- 简单描述
- 双染蛋白MARKERPrecision Plus Protein™ Dual Color Stand500 μl, mixture of 10 recombinant proteins (10–250 kD), 8 blue-stained bands, and 2 pink reference bands (25 and 75 kD), 50 applications
详细介绍
双染蛋白MARKERPrecision Plus Protein™ Dual Color Stand
Precision Plus Protein™ Dual Color Standards #161-0374
500 μl, mixture of 10 recombinant proteins (10–250 kD), 8 blue-stained bands, and 2 pink reference bands (25 and 75 kD), 50 applications
- Description
- 技术指标
MW size range, kD | 10–250 |
Stain type | Dual Color |
Volume, µl | 500 |
Total number of applications | 50 |
Shelf life at –20°C, months | 12 |
Loading volume on mini gels, µl | for Coomassie staining, 10 |
Loading volume on mini gels, µl | for fluorescent blot detection, 5 of 1:10 dilution |
Application Type | |
Monitoring electrophoretic separation for SDS-PAGE gels | Yes |
Molecular weight sizing on SDS-PAGE gels | Yes |
Monitoring blot transfer efficiency | Yes |
Fluorescence blots | Yes |
Molecular weight sizing on | N/A |
Loading Volume on Mini Gels | |
For Coomassie staining, µl | N/A |
For stain-free gels, µl | N/A |
For silver staining, µl | N/A |
For SYPRO Ruby staining, µl | N/A |
For blot development, µl | N/A |
StrepTactin conjugate working dilutions for colorimetric blot development | N/A |
StrepTactin conjugate working dilutions for chemiluminescence blot development | N/A |
The Precision Plus Protein™ Dual Color standards are a mixture of ten recombinant proteins (10–250 kD), including eight blue-stained bands and two pink reference bands (25 and 75 kD).
双染蛋白MARKERPrecision Plus Protein™ Dual Color Stand
Applications and Uses
Choose Precision Plus Protein Dual Color standards for SDS-PAGE:
- Monitoring electrophoretic separation
- Molecular weight sizing
- Monitoring blot transfer efficiency with easy orientation via multicolor standards
- Fluorescent blot detection, with blue bands visualized in the red wavelength range and pink bands in the green wavelength (prepare a 1:10 dilution of the Dual Color standard and load 5 µl per well)
More Information
Loading buffer:
30% (w/v) glycerol, 2% SDS, 62.5 mM Tris, pH 6.8, 50 mM DTT, 5 mM EDTA, 0.02% NaN3, 0.01% bromophenol blue