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FeBABE
FeBABE
- 分子生物学関連試薬
- 二価性試薬
生体高分子機能解析用試薬
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製品コードF279 FeBABE
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CAS番号186136-50-5
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化学名1-(p-Bromoacetamidobenzyl)ethylenediamine-N,N,N’,N’-tetraacetic acid, iron(III)
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分子式・分子量C19H21BrFeN3O9=571.13
| 容 量 | メーカー希望 小売価格 |
富士フイルム 和光純薬 |
|---|---|---|
| 1 mg | ￥18,300 | 345-08641 |
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技術情報
溶解例
緩衝溶液、DMSOに可溶
参考文献
1) L. H. DeRiemer, C. F. Meares, D. A. Goodwin and C. I. Diamanti, "BLEDTA II: Synthesis of a New Tumer-Visualizing Derivative of Co(III)-bleomycin", J. Labelled Compd. Radiopharm., 1981, 18, 1517.
2) T. M. Rana and C. F. Meares, "Specific Cleavage of a Protein by an Attached Iron Chelate", J. Am. Chem. Soc., 1990, 112, 2457.
3) T. M. Rana and C. F. Meares, "Transfer of Oxigen from an artificial protease to peptide carbon during proteolysis", Proc. Natl. Acad. Sci. USA, 1991, 88, 10578.
4) D. P. Greiner, R. Miyake, J. K. Moran, A. D. Jones, T. Negishi, A. Ishihama and C. F. Meares, "Synthesis of the Protein Cutting Reagent Iron (S)-1-(p-Bromoacetamidobenzyl)ethylebediaminetetraacetate and Conjuation to cysteine Sie Cahins", Bioconjugate Chem., 1997, 8, 44.
5) E. Platis, M. R. Ermacora and R. O. Fox, "Oxidative Polypeptide Cleavage Mediated by EDTA-Fe Covalently Linked to Cysteine Residue", Biochemistry, 1993, 32, 12761.
6) S. L. Traviglia, S. A. Datwyler, D. Yan, A. Ishihama and C. F. Meares, "Targeted Protein Footprinting: Where Different Transcription Factors bind to RNA Polymerase", Biochemistry, 1999, 38, 4259.
7) J. B. Ghaim, D. P. Greiner, C. F. Meares and R. B. Gennis, "Proximity Mapping the suface of Membrane Protein Using an Artificial Protease: Demonstration That the Quinone-Binding Domain of Subunit I Is near the N-Terminal Region of Subunit II of Cytochrome bd", Biochemistry, 1995, 34, 11311.
8) R. Miyake, K. Murakami, J. T. Owens, D. P. Greiner, O. N. Ozoline, A. Ishihama and C. F. Meares, "Dimeric Association of Escherichia coli RNA Polymerase alfa subunits, studied by Cleavage of Single-Cysteine alfa Sununits Conjugated to Iron-(S)-1-(p-(Bromoacetamido)benzyl)ethylenediaminetetraacetate", Biochemistry, 1998, 37, 1344.
9) J. T. Owens, R. Miyake, K. Murakami, A. J. Chmura, N. Fujita, A. Ishihama and C. F. Meares, "Mapping the sigma70 subunits contact sites on Escherichia coli RNA polymerase with a sigma70-conjugated chemical protease", Proc. Natl. Acad. Sci. USA, 1998, 95, 6021.
10) J. A. Bown, J. T. Owens, C. F. Meares, N. Fujita, A. Ishihama, S. J. Busby and S. D. Minchin, "Organization of open complexes at Escherichia coli promoters. Location of promoter DNA sites close to region 2.5 of the sigma70 subunit of RNA polymerase", J. Biol. Chem., 1999, 274, 2263.
11) F. Colland, N. Fujita, D. Kotlarz, J. A. Bown, C. F. Meares, A. Ishihama and A. Kolb, "Positioning of sigma(S), the stationary phase sigma factor, in Escherichia coli RNA polymerase-promoter open complexes", EMBO J., 1999, 18, 4049.
12) G. M. Heilek, R. Marusak, C. F. Meares and H. F. Noller, "Directed hydroxyl radical probing of 16S rRNA using Fe(II) tethered to ribosomal protein S4", Proc. Natl. Acad. Sci. USA, 1995, 92, 1113.
13) G. M. Heilek and H. F. Noller, "Site-directed hydroxyl radical probing of the rRNA neighborhood of ribosomal protein S5", Science, 1996, 272, 1659.
14) K. R. Lieberman and H. F. Noller, "Ribosomal protein L15 as a probe of 50 S ribosomal subunit structure.", J. Mol. Biol., 1998, 284, 1367.
取扱条件
| 性状： | 本品は、黄褐色粉末である。 |
|---|---|
| 純度(HPLC)： | 95.0％ 以上 |
| 水溶状： | 試験適合 |
| 保存条件: 冷凍 , 取扱条件: 吸湿注意 |
