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NEBNext Ultra II RNA 定向第二链合成模块 收藏
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概述
The NEBNext® Ultra™ II Directional RNA Second Strand Synthesis Module has been optimized to
generate double-stranded cDNA from first-strand cDNA, as part of the NEBNext Directional RNA library
preparation workflow. This workflow uses the “dUTP” method for strand-specific library construction, which
depends on incorporation of uracil into the second strand cDNA, enabled by the NEBNext Second Strand
Synthesis Reaction Buffer with dUTP Mix, included in this module.
The NEBNext Ultra II Directional RNA Second Strand Synthesis Module is Designed for Use with the
Following:
– NEBNext® Ultra™ II RNA First Strand Synthesis Module (NEB #E7771)
– NEBNext® Ultra™ II End Repair/dA-Tailing Module (NEB #E7546)
– NEBNext® Ultra™ II Ligation Module (NEB #E7595)
– NEBNext® Ultra™ II Q5® Master Mix (NEB #M0544)
– NEBNext® Oligos for Illumina®
#E7535, #E7350)
随酶提供的试剂
NEBNext Second Strand Synthesis Enzyme Mix
NEBNext Second Strand Synthesis Reaction Buffer with dUTP Mix
贮存温度
-20°C
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