iPS Cell to Endothelial Cell Differentiation System酶试剂盒Takara


iPS Cell to Endothelial Cell Differentiation System
品牌 Code No. 产品名称 包装量 价格(元) 说明书 数量
Cellartis Y50300 MiraCell® iPS Cell to Endothelial Cell Differentiation Kit 1 Kit ¥13,273 iPS Cell to Endothelial Cell Differentiation System iPS Cell to Endothelial Cell Differentiation System iPS Cell to Endothelial Cell Differentiation System
Cellartis Y50301 MiraCell® iPS Cell to Endothelial Cell Differentiation Kit with DEF-CS 500 Culture System 1 Kit ¥18,627 iPS Cell to Endothelial Cell Differentiation System iPS Cell to Endothelial Cell Differentiation System iPS Cell to Endothelial Cell Differentiation System
Cellartis Y50053 MiraCell® EC Culture Medium 500 ml ¥4,148 iPS Cell to Endothelial Cell Differentiation System iPS Cell to Endothelial Cell Differentiation System iPS Cell to Endothelial Cell Differentiation System
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iPS向血管内皮细胞定向分化系统
(iPS Cell to Endothelial Cell Differentiation System)
MiraCell iPS Cell to Endothelial Cell Differentiation Kit用于人iPS细胞向血管内皮细胞定向分化诱导,试剂盒中包含2种包被剂、2种分化用基础培养基和6种培养基添加剂,足够支持1个T25培养瓶 (1.5×105 Cells)或3个6孔板单孔(1.8×105 cells)的iPS细胞进行内皮方向分化诱导,分化周期为12天(Day0至Day11)。建议分化起始的iPS细胞先使用Cellartis® DEF-CS 500 Culture System(Y30010)驯化3-5代,以确保iPS细胞处于良好的多能性状态。
诱导分化获得的高纯度(CD31阳性率>80%*)血管内皮细胞,经鉴定表达CD31、CD144、Tie2、VEGFR2等血管内皮标志物和具备小管(Tube)形成能力。分化好的血管内皮细胞(Day12以后)可以使用MiraCell EC Culture Medium(Y50053)长期扩大培养,也可以冷冻保存备用。(*起始的人iPS细胞株不同,得到的血管内皮细胞性能可能不同。)
 
本产品使用京都大学iPS细胞研究所开发的人血管内皮细胞的制备技术,由Takara和iHeart Japan公司共同研发,由Takara制造并销售。
 
■ 产品特点
· 完整的试剂盒,支持人iPS细胞-中胚层细胞-血管内皮细胞定向分化
· 多个iPS细胞株测试验证,支持高效分化诱导(>80%)
· 无需基因修饰和药物筛选过程,操作简便
· 获得的血管内皮细胞,可冷冻保存和扩大培养
 
■ 产品组成
MiraCell® iPS Cell to Endothelial Cell Differentiation Kit,Y50300
· Coating Reagent 1 (Day0 & 2);Coating Reagent 2 (Day11)
· EC Differentiation Basal Medium 1 (Day3~9);EC Differentiation Basal Medium 2 (Day11)
· Supplement 1 (Day3);Supplement 2 (Day4);Supplement 3 (Day7);Supplement 4 (Day8);Supplement 5 (Day9);Supplement 6 (Day11)
 
iPS Cell to Endothelial Cell Differentiation System
 
MiraCell® iPS Cell to Endothelial Cell Differentiation Kit with DEF-CS 500 Culture System,Y50301
· MiraCell® iPS Cell to Endothelial Cell Differentiation Kit,Y50300
· Cellartis® DEF-CS 500 Culture System,Y30010
 
iPS Cell to Endothelial Cell Differentiation System
 
■ 实验例
iPS Cell to Endothelial Cell Differentiation System
图1. 3种人iPS细胞株使用MiraCell iPS Cell to Endothelial Cell Differentiation Kit分化诱导,获得的血管内皮细胞的形态比较(A)和标志蛋白质(B)比较。
 
iPS Cell to Endothelial Cell Differentiation System
图2. 3种人iPS细胞株使用MiraCell iPS Cell to Endothelial Cell Differentiation Kit分化诱导,获得的血管内皮细胞继续使用MiraCell EC Culture Medium维持培养,可长时间维持高纯度。()内的数值表示Day32的细胞扩大倍数,以Day8的细胞数为1。
 
iPS Cell to Endothelial Cell Differentiation System
图3. 冷冻保存的、使用MiraCell iPS Cell to Endothelial Cell Differentiation Kit分化诱导的血管内皮细胞(Day16),在解冻复苏培养Day8后的小管形成实验结果。
 
 
 
产品详情请点击:http://catalog.takara-bio.co.jp/product/basic_info.php?unitid=U100009383
 
 

页面更新:2020-02-13 14:58:53