CycleavePCR Reaction Mix (2×Conc.)*1 |
1 ml |
ROX Reference Dye (50×Conc.)*2 |
200 μl |
ROX Reference Dye II (50×Conc.)*2 |
200 μl |
Positive Control*3 |
10 μl |
Positive Control Primer Mix (10 μM each)*3 |
10 μl |
Positive Control Probe (FAM/TAMRA) (25×conc.)*3*4 |
10 μl |
dH2O |
1 ml |
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*1 含有TaKaRa Ex Taq HS,dNTP Mixture,Mg2+,Tli RNaseH II。 *2 使用在Applied Biosystems的Real Time PCR扩增仪上,用以校正孔与孔之间的荧光信号误差。 ◆ 需要添加ROX Reference Dye的仪器 ·StepOnePlus Real-Time PCR System(Thermo Fisher Scientific) ◆ 需要添加ROX Reference Dye II的仪器 ·Applied Biosystems 7500/7500 Fast Real-Time PCR System(Thermo Fisher Scientific) ◆ 不需要添加ROX Reference Dye或ROX Reference Dye II的仪器 ·Thermal Cycler Dice Real Time System系列(Code No. TP950等) ·Smart Cycler System/Smart Cycler II System(Cepheid) ·LightCycler(Roche Diagnostics) *3 用于对照实验,确认试剂反应性能。 *4 荧光标记探针应避光保存。 |
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